Evidence for 1,8-dihydroxynaphthalene melanin in three halophilic black yeasts grown under saline and non-saline conditions.

abstract：:Microcystins produced by cyanobacteria are potent and specific hepatotoxins; however, the mechanisms of microcystin-induced hepatotoxicity have not been fully elucidated. The induction of free radical formation and mitochondrial alterations are two major events found in microcystin-treated cultured rat hepatocytes. Th...

journal_title：FEMS microbiology letters

authors： Ding WX,Nam Ong C

更新日期：2003-03-14 00:00:00

abstract：:In addition to core Cas proteins, CRISPR-Cas loci often encode ancillary proteins that modulate the activity of the respective effectors in interference. Subtype VI-B1 CRISPR-Cas systems encode the Csx27 protein that down-regulates the activity of Cas13b when the type VI-B locus is expressed in Escherichia coli. We sh...

journal_title：FEMS microbiology letters

authors： Makarova KS,Gao L,Zhang F,Koonin EV

更新日期：2019-04-01 00:00:00

abstract：:The NH(2)-terminal amino acid sequence of L-threo-3-hydroxyaspartate dehydratase from Pseudomonas sp. T62 showed significant similarity to that of the SRY1 gene product of Saccharomyces cerevisiae (serine racemase in yeast). SRY1 was cloned and expressed in Escherichia coli, and the gene product was purified and parti...

journal_title：FEMS microbiology letters

authors： Wada M,Nakamori S,Takagi H

更新日期：2003-08-29 00:00:00

abstract：:Molecular information suggests that there is a broad diversity of acetogens in the rumen, distinct from any currently isolated acetogens. We combined molecular analysis with enrichment culture techniques to investigate this diversity further. Methane-inhibited, hydrogenotrophic enrichment cultures produced acetate as ...

journal_title：FEMS microbiology letters

authors： Gagen EJ,Padmanabha J,Denman SE,McSweeney CS

更新日期：2015-07-01 00:00:00

abstract：:Three (0140J, C197C and EF20) out of four strains of Streptococcus uberis exhibited high levels of bound plasmin activity following growth in the presence of bovine plasminogen. The remaining strain (C197) bound considerably less plasmin following growth in the same medium. In contrast to the others, this strain was u...

journal_title：FEMS microbiology letters

authors： Leigh JA,Lincoln RA

更新日期：1997-09-01 00:00:00

abstract：:This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

journal_title：FEMS microbiology letters

authors： Nastos S,Rangachari PK

更新日期：2016-08-01 00:00:00

abstract：:The c-type cytochrome (OmcB) and the multicopper protein (OmpB) required for Fe(III) oxide reduction by Geobacter sulfurreducens were predicted previously to be outer membrane proteins, but it is not clear whether they are positioned in a manner that permits the interaction with Fe(III). Treatment of whole cells with ...

journal_title：FEMS microbiology letters

authors： Qian X,Reguera G,Mester T,Lovley DR

更新日期：2007-12-01 00:00:00

abstract：:Legionella pneumophila is known as a facultative intracellular parasite of free-living soil and freshwater amoebae, of which several species have been shown to support the growth of the pathogenic bacteria. We report for the first time the behaviour of two strains (c2c and Z503) of the amoeba Willaertia magna towards ...

journal_title：FEMS microbiology letters

authors： Dey R,Bodennec J,Mameri MO,Pernin P

更新日期：2009-01-01 00:00:00

abstract：:The replication region of plasmid pRA2 from Pseudomonas alcaligenes NCIB 9867 (strain P25X) was localized within a 5.9-kbp DNA fragment and its sequence was determined. An interesting feature of the sequence is the presence of a 1.3-kbp region containing seven, highly conserved, direct repeats of 72 bp in length. The ...

journal_title：FEMS microbiology letters

authors： Kwong SM,Yeo CC,Chuah D,Poh CL

更新日期：1998-01-15 00:00:00

abstract：:Strains of Salmonella enteritidis expressed a novel porin with a subunit size of 35.5 kDa as shown by SDS-PAGE. This protein was expressed as a major outer membrane protein (MOMP) when grown on nutrient agar, McConkey agar or blood agar, or in Tris-succinate medium; but was only produced in trace amounts when strains ...

journal_title：FEMS microbiology letters

authors： Chart H,Frost JA,Rowe B

更新日期：1993-05-15 00:00:00

abstract：:DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indicated that Pab PCR perf...

journal_title：FEMS microbiology letters

authors： Dietrich J,Schmitt P,Zieger M,Preve B,Rolland JL,Chaabihi H,Gueguen Y

更新日期：2002-11-19 00:00:00

abstract：:Comparisons of growth and surface colonisation of Enterococcus faecium L-forms and their cell-walled forms were undertaken to produce information about their ability to form sessile cells. The growth of L-forms in liquid culture was slower than that of the parent. This was reflected in their longer lag phase and slowe...

journal_title：FEMS microbiology letters

authors： Jass J,Phillips LE,Allan EJ,Costerton JW,Lappin-Scott HM

更新日期：1994-01-15 00:00:00

abstract：:The outer membrane proteins of several prominent bacterial pathogens demonstrate substantial variation in their surface antigenic epitopes. To determine if this was also true for Pseudomonas aeruginosa outer membrane protein OprF, gene sequencing of a serotype 5 isolate was performed to permit comparison with the publ...

journal_title：FEMS microbiology letters

authors： Martin NL,Rawling EG,Wong RS,Rosok M,Hancock RE

更新日期：1993-11-01 00:00:00

abstract：:Peroxidase-conjugated transferrin was used to detect transferrin receptors both in intact outer membrane vesicles (OMVs) from Neisseria species in a dot blot assay, and in SDS-PAGE-separated OMV proteins after transferring to nitrocellulose membranes. All N. meningitidis strains produced transferrin receptors after cu...

journal_title：FEMS microbiology letters

authors： Ferreirós CM,Criado MT,Pintor M,Ferrón L

更新日期：1991-10-15 00:00:00

abstract：:In Corynebacterium glutamicum the promoter of the araBAD Escherichia coli gene is positively regulated by both arabinose and the araC gene product, as it is the case in the natural host. If the L-arabinose inducer and an active araC gene are present, significant amounts of araBAD promoter expression take place in the ...

journal_title：FEMS microbiology letters

authors： Ben-Samoun K,Leblon G,Reyes O

更新日期：1999-05-01 00:00:00

abstract：:Little is known about the diversity and roles of Gram-positive and pigmented bacteria in Antarctic environments, especially those associated with marine macroorganisms. This work is the first study about the diversity and antimicrobial activity of culturable pigmented Gram-positive bacteria associated with marine Anta...

journal_title：FEMS microbiology letters

authors： Leiva S,Alvarado P,Huang Y,Wang J,Garrido I

更新日期：2015-12-01 00:00:00

abstract：:Helicobacter pylori genomes contain about 30 hop genes that encode outer membrane proteins. Helicobacter pylori hopQ alleles exhibit a high level of genetic diversity, and two families of hopQ alleles have been described. Type I hopQ alleles are found more commonly in cag-positive H. pylori strains from patients with ...

journal_title：FEMS microbiology letters

authors： Loh JT,Torres VJ,Algood HM,McClain MS,Cover TL

更新日期：2008-12-01 00:00:00

abstract：:The bovine enteric calici-like virus, Newbury agent 1 (NA1) was characterised to determine if it is a member of the Caliciviridae and to establish its antigenic relationship to the established bovine enteric calicivirus Newbury agent 2 (NA2). Solid phase immune electron microscopy (SPIEM) allowed quantification of NA1...

journal_title：FEMS microbiology letters

authors： Dastjerdi AM,Snodgrass DR,Bridger JC

更新日期：2000-11-01 00:00:00

abstract：:Escherichia coli has been used widely in laboratory and the biotech industry. However, the genetic and metabolic characteristics remain inadequately studied, particularly for those strains with extensive genetic manipulations that might have resulted in unknown mutations. Here, we demonstrate a comparative proteomics ...

journal_title：FEMS microbiology letters

authors： Xia XX,Qian ZG,Lee SY

更新日期：2011-01-01 00:00:00

abstract：:Phytochromes are red/far-red light photoreceptors found in plants, cyanobacteria and heterotrophic bacteria. Biochemical analyses have established that the genes bphO and bphP (PA4116 and PA4117) of Pseudomonas aeruginosa encode both phytochrome components: BphO, a heme oxygenase that produces the linear tetrapyrrole ...

journal_title：FEMS microbiology letters

authors： Barkovits K,Harms A,Benkartek C,Smart JL,Frankenberg-Dinkel N

更新日期：2008-03-01 00:00:00

abstract：:We have identified phosphatidylethanolamine as one of the major phospholipids of Clostridium perfringens by two dimensional thin layer chromatography of the intact lipids and of their deacylation products and by liquid chromatography followed by mass spectrometry of the intact neutral phospholipid fraction. The princi...

journal_title：FEMS microbiology letters

authors： Johnston NC,Baker JK,Goldfine H

更新日期：2004-04-01 00:00:00

abstract：:The major heat shock proteins from Thiobacillus ferrooxidans were identified as DnaK and GroEL equivalents by Western blotting and analysis of the N-terminal amino acid sequence of spots isolated from dried 2-D polyacrylamide electrophoresis gels. The T. ferrooxidans chaperonins showed 70% and 80% identity with the Es...

journal_title：FEMS microbiology letters

authors： Varela P,Jerez CA

更新日期：1992-11-01 00:00:00

abstract：:The flaA gene, which codes for the Spirochaeta aurantia flagellar filament outer layer polypeptide, FlaA, was cloned, sequenced and analysed. The gene appears to be transcribed into a monocistronic mRNA from a sigma 70-like promoter. The translational start is 31 base pairs after the start of the transcript, the open ...

journal_title：FEMS microbiology letters

authors： Parales J Jr,Greenberg EP

更新日期：1993-02-01 00:00:00

abstract：:To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More i...

journal_title：FEMS microbiology letters

authors： Majcherczyk PA,McKenna T,Moreillon P,Vaudaux P

更新日期：2006-02-01 00:00:00

abstract：:The natural life cycle of Vibrio cholerae involves the transitioning of cells between different environmental surfaces such as the chitinous shell of Crustaceae and the epithelial layer of the human intestine. Previous studies using static biofilm systems showed a strict dependence of biofilm formation on the vps and ...

journal_title：FEMS microbiology letters

authors： Müller J,Miller MC,Nielsen AT,Schoolnik GK,Spormann AM

更新日期：2007-10-01 00:00:00

abstract：:Twitching motility is a form of bacterial translocation over solid or semi-solid surfaces mediated by the extension, tethering, and subsequent retraction of type IV pili. These pili are also known to be involved in virulence, biofilm formation, formation of fruiting bodies, horizontal gene transfer, and protein secret...

journal_title：FEMS microbiology letters

authors： Coil DA,Anné J

更新日期：2009-04-01 00:00:00

abstract：:Two Pseudomonas aeruginosa genes, termed phaJ1(Pa) and phaJ2(Pa), homologous to the Aeromonas caviae (R)-specific enoyl-CoA hydratase gene (phaJ(Ac)) were cloned using a PCR technique to investigate the monomer-supplying ability for polyhydroxyalkanoate (PHA) synthesis from beta-oxidation cycle. Two expression plasmid...

journal_title：FEMS microbiology letters

authors： Tsuge T,Fukui T,Matsusaki H,Taguchi S,Kobayashi G,Ishizaki A,Doi Y

更新日期：2000-03-15 00:00:00

abstract：:The 42- and 51-kDa protein genes of Bacillus sphaericus 1593 have been subcloned independently downstream from the cytA gene promoter of Bacillus thuringiensis serovar israelensis and introduced into a non-mosquitocidal strain of Bacillus thuringiensis. Consequently, each protein was overproduced and accumulated as in...

journal_title：FEMS microbiology letters

authors： Nicolas L,Nielsen-Leroux C,Charles JF,Delécluse A

更新日期：1993-02-01 00:00:00

abstract：:The structural gene, adhA, for a thermostable primary alcohol dehydrogenase was cloned from Thermoanaerobacter ethanolicus JW200. Constitutive expression from its own promoter was observed in Escherichia coli. The nucleotide sequence of adhA corresponded to an open reading frame of 1197 bp, encoding a polypeptide of 3...

journal_title：FEMS microbiology letters

authors： Holt PJ,Williams RE,Jordan KN,Lowe CR,Bruce NC

更新日期：2000-09-01 00:00:00

abstract：:Blocking glycolytic breakdown of glucose by inactivation of phosphoglucose isomerase (Pgi) in Escherichia coli led to a greatly reduced maximum specific growth rate. Examination of the operational catabolic pathways and their flux ratios using [U-(13)C(6)]glucose-labeling experiments and metabolic flux ratio analysis ...

journal_title：FEMS microbiology letters

authors： Canonaco F,Hess TA,Heri S,Wang T,Szyperski T,Sauer U

更新日期：2001-11-13 00:00:00