Location of catalase in crystalline peroxisomes of methanol-grown Hansenula polymorpha.

Abstract:

:We have studied the intraperoxisomal location of catalase in peroxisomes of methanol-grown Hansenula polymorpha by (immuno)cytochemical means. In completely crystalline peroxisomes, in which the crystalline matrix is composed of octameric alcohol oxidase (AO) molecules, most of the catalase protein is located in a narrow zone between the crystalloid and the peroxisomal membrane. In non-crystalline organelles the enzyme was present throughout the peroxisomal matrix. Other peroxisomal matrix enzymes studied for comparison, namely dihydroxyacetone synthase, amine oxidase and malate synthase, all were present throughout the AO crystalloid. The advantage of location of catalase at the edges of the AO crystalloids for growth of the organism on methanol is discussed.

journal_name

FEMS Microbiol Lett

authors

Keizer I,Roggenkamp R,Harder W,Veenhuis M

doi

10.1016/0378-1097(92)90481-3

keywords:

subject

Has Abstract

pub_date

1992-05-15 00:00:00

pages

7-11

issue

1

eissn

0378-1097

issn

1574-6968

journal_volume

72

pub_type

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