Inhibition of Neurospora crassa cytosolic chitinase by allosamidin.

Abstract:

:A cytosolic chitinase (20 kDa by SDS-PAGE) was partially purified from Neurospora crassa. Linear kinetics for enzyme activity were obtained using the substrate [3H]-labelled regenerated chitin, the preparation yielding an apparent Km of 0.965 mg ml-1 and a Vmax of 3.83 micrograms GlcNAc min-1 (mg protein)-1. The enzyme was highly sensitive to allosamidin, an inhibitor of insect chitinase, exhibiting an IC50 of 1.6 microM. Unlike other chitinases that are inhibited by allosamidin, the mode of inhibition of the N. crassa enzyme was shown to be non-competitive.

journal_name

FEMS Microbiol Lett

authors

McNab R,Glover LA

doi

10.1016/0378-1097(91)90424-9

subject

Has Abstract

pub_date

1991-07-15 00:00:00

pages

79-82

issue

1

eissn

0378-1097

issn

1574-6968

journal_volume

66

pub_type

杂志文章
  • Infection by the 'photosynthetic' phage S-PM2 induces increased synthesis of phycoerythrin in Synechococcus sp. WH7803.

    abstract::Phycoerythrin-containing Synechococcus strains are unicellular cyanobacteria that are of great ecological importance in the marine environment. These organisms are known to be susceptible to infection by cyanophages (viruses that infect cyanobacteria). The infection cycle takes several hours and during this time the c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01148.x

    authors: Shan J,Jia Y,Clokie MR,Mann NH

    更新日期:2008-06-01 00:00:00

  • A novel colorimetric method for the detection of Escherichia coli using cytochrome c peroxidase-encoding bacteriophage.

    abstract::A new rapid and simple method was developed for the detection of Escherichia coli by constructing a recombinant T4 phage carrying the cytochrome c peroxidase gene derived from Saccharomyces cerevisiae (T4ccp) using which, the colorimetric detection of E. coli K12 was examined. The oxidation activity toward the chromog...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12382

    authors: Hoang HA,Abe M,Nakasaki K

    更新日期:2014-03-01 00:00:00

  • Construction of a fusion protein carrying antigenic determinants of enteric clostridial toxins.

    abstract::Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00560-3

    authors: Belyi IF,Varfolomeeva NA

    更新日期:2003-08-29 00:00:00

  • Allelic divergence at B alpha 1 pheromone receptor genes of Schizophyllum commune.

    abstract::The multiallelic mating type locus B alpha 1 of Schizophyllum commune encodes a pheromone receptor and putative pheromone genes. A comparison of two alleles encoding receptors that share the same specificity B alpha 1 was performed using strains of different geographic origin. The amino acid sequence alignment reveale...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08615.x

    authors: Wendland J,Kothe E

    更新日期:1996-12-15 00:00:00

  • A surface polysaccharide forms when gonococci are converted to serum resistance by cytidine 5'-monophospho-N-acetyl neuraminic acid.

    abstract::A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90261-n

    authors: Fox AJ,Curry A,Rowland PL,Lancaster S,Jones DM,Parsons NJ,Cole JA,Smith H

    更新日期:1990-01-01 00:00:00

  • Induction of a viable but non-culturable state in Salmonella Typhimurium by thermosonication and factors affecting resuscitation.

    abstract::The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx249

    authors: Liao H,Jiang L,Zhang R

    更新日期:2018-02-01 00:00:00

  • Quantification of Agrobacterium tumefaciens C58 attachment to Arabidopsis thaliana roots.

    abstract::Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony coun...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx158

    authors: Petrovicheva A,Joyner J,Muth TR

    更新日期:2017-10-02 00:00:00

  • Non-radioactive restriction fragment length polymorphism (RFLP) typing of Clostridium difficile.

    abstract::A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemilumine...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90097-t

    authors: Bowman RA,O'Neill GL,Riley TV

    更新日期:1991-04-15 00:00:00

  • A species of magnetotactic deltaproteobacterium was detected at the highest abundance during an algal bloom.

    abstract::Magnetotactic bacteria (MTB) are a group of microorganisms that have the ability to synthesize intracellular magnetic crystals (magnetosomes). They prefer microaerobic or anaerobic aquatic sediments. Thus, there is growing interest in their ecological roles in various habitats. In this study we found co-occurrence of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz253

    authors: Pan H,Dong Y,Teng Z,Li J,Zhang W,Xiao T,Wu LF

    更新日期:2019-11-01 00:00:00

  • Phylogenetic analysis of polycyclic aromatic hydrocarbon degrading mycobacteria by 16S rRNA sequencing.

    abstract::Mycobacterium sp. PYR-1 was previously isolated in our laboratory and was shown to be able to mineralize high molecular mass polycyclic aromatic hydrocarbons (PAHs) [Heitkamp and Cerniglia, (1988) Appl. Environ. Microbiol. 54, 1612-1614]. In this research, the 16S rRNA gene (rDNA) of this strain was amplified by polym...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(95)00186-9

    authors: Wang RF,Cao WW,Cerniglia CE

    更新日期:1995-07-15 00:00:00

  • Degradation of homovanillate by a strain of Variovorax paradoxus via ring hydroxylation.

    abstract::A newly isolated strain of Variovorax paradoxus could grow on homovanillate and several monohydroxylated phenylacetic acids. During growth on homovanillate, the organism formed separate NAD(P)H-dependent hydroxylases with activity towards 4-hydroxyphenylacetic acid and homovanillate. Homovanillate hydroxylase catalyse...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07940.x

    authors: Allison N,Turner JE,Wait R

    更新日期:1995-12-15 00:00:00

  • Envelope ultrastructure of uncultured naturally occurring magnetotactic cocci.

    abstract::Magnetotactic bacteria are microorganisms that respond to magnetic fields. We studied the surface ultrastructure of uncultured magnetotactic cocci collected from a marine environment by transmission electron microscopy using freeze-fracture and freeze-etching. All bacteria revealed a Gram-negative cell wall. Many bact...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(02)01187-4

    authors: Freitas F,Keim CN,Kachar B,Farina M,Lins U

    更新日期:2003-02-14 00:00:00

  • Inhibition of adhesion-promoting activity of a human salivary protein which promotes adhesion of Streptococcus mutans JBP to hydroxyapatite.

    abstract::The adhesion-promoting proteins (APP) (molecular mass approx. 300 kDa), which promote adhesion of Streptococcus mutans JBP (serotype c) to hydroxyapatite, were isolated from human submandibular-sublingual (SMSL) saliva by gel filtration on a Trisacryl GP2000 M column. The effects of hexoses, pentoses, methyl-pentoses,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90639-r

    authors: Kishimoto E,Hay DI,Gibbons RJ

    更新日期:1991-12-15 00:00:00

  • A new pullulan and a branched (1-->3)-, (1-->6)-linked beta-glucan from the lichenised ascomycete Teloschistes flavicans.

    abstract::The polysaccharides formed on hot alkaline extraction of the ascomycetous lichen Teloschistes flavicans were fractionated to give two glucans, which were characterised by methylation analysis and 1D and 2D NMR spectroscopy. One was a branched beta-glucan containing (1-->3) and (1-->6) linkages, a structure which is mo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11152.x

    authors: Reis RA,Tischer CA,Gorin PA,Iacomini M

    更新日期:2002-04-23 00:00:00

  • Taxonomic revision of the cellulose-degrading fungus Acremonium cellulolyticus nomen nudum to Talaromyces based on phylogenetic analysis.

    abstract::The cellulase-producing fungal strain Y-94, isolated in Japan and invalidly described as Acremonium cellulolyticus nom. nud. strain Y-94, seldom forms enteroarthric conidia under nutrient starvation conditions. Phylogenetic analysis using ITS1-5.8S-ITS2 and RNA polymerase II large subunit gene sequences revealed that ...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12352

    authors: Fujii T,Hoshino T,Inoue H,Yano S

    更新日期:2014-02-01 00:00:00

  • Characterization of extended-spectrum-beta-lactamase (CTX-M-15)-producing strains of Salmonella enterica isolated in France and Senegal.

    abstract::Three clinical isolates of Salmonella enterica recovered between 2000 and 2003 in France and Senegal were found to produce extended-spectrum beta-lactamase CTX-M-15. The two isolates from Senegal were recovered from stool of a hospitalized patient with gastroenteritis in 2000 and from an urine specimen of an out-patie...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.07.058

    authors: Weill FX,Perrier-Gros-Claude JD,Demartin M,Coignard S,Grimont PA

    更新日期:2004-09-15 00:00:00

  • Rapid identification and quantification of Collinsella aerofaciens using PCR.

    abstract::The number and incidence of Collinsella aerofaciens in the human intestine are the highest among Gram-positive non-spore-forming bacilli. Identification of this species is very difficult and requires considerable time. A PCR-based identification system using C. aerofaciens-specific primers is described. Using this PCR...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08931.x

    authors: Kageyama A,Sakamoto M,Benno Y

    更新日期:2000-02-01 00:00:00

  • Genetic evidence for a novel thymidylate synthase in the halophilic archaeon Halobacterium salinarum and in Campylobacter jejuni.

    abstract::A search of the complete genome sequence of the halophilic archaeon Halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thyA) gene present in the closely related Haloferax volcanii. To understand the source of thymidine synthesis in Hbt. salinarum, a genomic library of Hbt. salina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11422.x

    authors: Giladi M,Bitan-Banin G,Mevarech M,Ortenberg R

    更新日期:2002-10-29 00:00:00

  • Denitrifying haloarchaea: sources and sinks of nitrogenous gases.

    abstract::Haloarchaea thrive under saline and hypersaline conditions and often dominate microbial communities in saltmarshes, salted lakes/soils and some oceanic areas. Some of the predominant species show denitrifying capabilities, although it remains unclear whether they are complete or partial denitrifiers. As complete denit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnx270

    authors: Torregrosa-Crespo J,Bergaust L,Pire C,Martínez-Espinosa RM

    更新日期:2018-02-01 00:00:00

  • An endophytic/pathogenic Phoma sp. from creosote bush producing biologically active volatile compounds having fuel potential.

    abstract::A Phoma sp. was isolated and characterized as endophytic and as a pathogen of Larrea tridentata (creosote bush) growing in the desert region of southern Utah, USA. This fungus produces a unique mixture of volatile organic compounds (VOCs), including a series of sesquiterpenoids, some alcohols and several reduced napht...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02297.x

    authors: Strobel G,Singh SK,Riyaz-Ul-Hassan S,Mitchell AM,Geary B,Sears J

    更新日期:2011-07-01 00:00:00

  • bkaR is a TetR-type repressor that controls an operon associated with branched-chain keto-acid metabolism in Mycobacteria.

    abstract::This study describes how bkaR, a highly conserved mycobacterial TetR-like transcriptional repressor, regulates a number of nearby genes that have associations with branched-chain keto-acid metabolism. bkaR (MSMEG_4718) was deleted from the nonpathogenic species Mycobacterium smegmatis, and changes in global gene expre...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12196

    authors: Balhana RJ,Swanston SN,Coade S,Withers M,Sikder MH,Stoker NG,Kendall SL

    更新日期:2013-08-01 00:00:00

  • tmRNA--a novel high-copy-number RNA diagnostic target--its application for Staphylococcus aureus detection using real-time NASBA.

    abstract::A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 other bacterial species. A detection limit of 1 cell equivalent wa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01822.x

    authors: O'Grady J,Lacey K,Glynn B,Smith TJ,Barry T,Maher M

    更新日期:2009-12-01 00:00:00

  • Growth and morphogenesis in Streptomyces.

    abstract::In this report we propose a model of apical growth for streptomycetes. The apical tip is considered as a multilayered wall that expands by an inside-to-outside mechanism of growth. It is also assumed that each layer is made up of peptidoglycan blocks, each of them being the result of the biosynthetic activity of a wal...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1992.tb14063.x

    authors: Miguélez EM,Martín C,Manzanal MB,Hardisson C

    更新日期:1992-12-15 00:00:00

  • Rapid accumulation of intracellular 2-keto-3-deoxy-6-phosphogluconate in an Entner-Doudoroff aldolase mutant results in bacteriostasis.

    abstract::The accumulation of 2-keto-3-deoxy-6-phosphogluconate, the key intermediate of the Entner-Doudoroff pathway, has long been thought to inhibit growth of bacteria, but careful measurements of 2-keto-3-deoxy-6-phosphogluconate accumulation by growing cells and the correlation of intracellular 2-keto-3-deoxy-6-phosphogluc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12870.x

    authors: Fuhrman LK,Wanken A,Nickerson KW,Conway T

    更新日期:1998-02-15 00:00:00

  • Purification and characterization of a membrane-associated ATPase from Natronococcus occultus, a haloalkaliphilic archaeon.

    abstract::Isolated membranes of the extreme haloalkaliphilic archaeon Natronococcus occultus were able to hydrolyze ATP via an ATPase, which required the presence of Mg(2+), high concentrations of NaCl, and a pH value of 9. The native molecular mass of the purified ATPase was 130 kDa and was composed of 74- and 61-kDa subunits....

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09232.x

    authors: Eddy ML,Jablonski PE

    更新日期:2000-08-15 00:00:00

  • H4 histone in the macronucleus of Blepharisma japonicum (Protozoa, Ciliophora, Heterotrichida).

    abstract::Two clones, obtained by polymerase chain reaction from macronuclear DNA of the unicellular ciliated protist Blepharisma japonicum, were isolated and sequenced. They correspond to fragments of two different putative H4 histone genes. The existence of multiple H4 histone genes was also suggested by Southern blot hybridi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb10314.x

    authors: Salvini M,Bini E,Santucci A,Batistoni R

    更新日期:1997-04-01 00:00:00

  • Towards biobased industry: acetate as a promising feedstock to enhance the potential of microbial cell factories.

    abstract::A broad range of different chemical and pharmaceutical compounds have been produced in microbial cell factories. To compete with traditional crude oil based production processes, the use of complex alternative raw materials such as lignocellulosic biomass, waste streams and utilization of CO2 in gas fermentations has ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny226

    authors: Novak K,Pflügl S

    更新日期:2018-10-01 00:00:00

  • Diversity of chromosomal AmpC beta-lactamases from Enterobacter cloacae isolates in a Portuguese hospital.

    abstract::Six clinical isolates of Enterobacter cloacae isolated in a Portuguese hospital, between April 1999 and November 2000, demonstrated resistance to almost all broad-spectrum cephalosporins, except to cefepime. These isolates were susceptible to quinolones and to aminoglycosides. Isoelectric focusing demonstrated product...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00891-7

    authors: Conceição T,Faria N,Lito L,Melo Cristino J,Salgado MJ,Duarte A

    更新日期:2004-01-30 00:00:00

  • Conformational plasticity of molecular chaperones involved in periplasmic and outer membrane protein folding.

    abstract::How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fny121

    authors: Mas G,Hiller S

    更新日期:2018-07-01 00:00:00

  • Water table related variations in the abundance of intact archaeal membrane lipids in a Swedish peat bog.

    abstract::The presence and distribution of isoprenoid glycerol dialkyl glycerol tetraethers (GDGTs), lipids that constitute the membranes of Archaea, have been investigated in a 50-cm long core from a Swedish peat bog. In the acrotelm, the periodically water saturated and thus oxic upper layer of the peat bog, only minor amount...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.08.012

    authors: Weijers JW,Schouten S,van der Linden M,van Geel B,Damsté JS

    更新日期:2004-10-01 00:00:00