Abstract:
:A gene encoding a phosphomannose isomerase from Streptococcus mutans GS-5 was identified immediately downstream from the fructokinase gene, scrK. Nucleotide sequence analysis of this region revealed an open reading frame (ORF) specifying a putative protein of 316 amino acids. The gene cloned in Escherichia coli expressed strong phosphomannose isomerase activity. The deduced amino acid sequence of the pmi gene has no significant similarity with any of the previously reported phosphomannose isomerase enzymes. Insertional inactivation of the upstream gene, scrK, in S. mutans also drastically reduced phosphomannose isomerase activity and the ability of the organism to utilize mannose as a sole carbon source. These results suggest that the S. mutans pmi gene constitutes an operon with the scrK gene.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Sato Y,Yamamoto Y,Kizaki H,Kuramitsu HKdoi
10.1111/j.1574-6968.1993.tb06551.xsubject
Has Abstractpub_date
1993-11-15 00:00:00pages
61-6issue
1eissn
0378-1097issn
1574-6968pii
0378-1097(93)90142-Ojournal_volume
114pub_type
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