Activity of the enzymes involved in the synthesis of exopolysaccharide precursors in an overproducing mutant ropy strain of Streptococcus thermophilus.


:The activities of some enzymes belonging to the Leloir pathway, phosphoglucomutase, UDP-glucose pyrophosphorylase, UDP-galactose 4-epimerase and galactose 1-P uridyl transferase, were studied in a wild ropy, a non-ropy and an overproducing mutant ropy strain of Streptococcus thermophilus. These activities were assayed over successive culture transfers along with exocellular polysaccharide (EPS) production. The overproducing mutant ropy strain showed increments in polysaccharide production over successive culture transfers, as opposed to reductions in production by the wild ropy strain. The observed variations among strains in the enzyme activities that were analysed in relation to EPS production suggest their involvement in the synthesis of sugar-nucleotide EPS precursors.


FEMS Microbiol Lett


Escalante A,Villegas J,Wacher C,García-Garibay M,Farrés A





Has Abstract


2002-04-09 00:00:00














  • Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes.

    abstract::In this study, we were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis with subpopulations of porcine white blood cells (WBC). The mutants included those with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and a mutant with five major pathogenicity islands remove...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Stepanova H,Volf J,Malcova M,Matiasovic J,Faldyna M,Rychlik I

    更新日期:2011-08-01 00:00:00

  • Expression of the ace operon in Escherichia coli is triggered in response to growth rate-dependent flux-signal of ATP.

    abstract::The signal that triggers the expression of the ace operon and, in turn, the transition of central metabolism's architecture from acetogenic to gluconeogenic in Escherichia coli remains elusive despite extensive research both in vivo and in vitro. Here, with the aid of flux analysis together with measurements of the en...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: El-Mansi M,Phue JN,Shiloach J

    更新日期:2021-01-08 00:00:00

  • Transposition of Tn4560 of Streptomyces fradiae in Mycobacterium smegmatis.

    abstract::Tn4560 (8.6 kb) was derived from Tn4556, a Tn3-like element from Streptomyces fradiae. It contains a viomycin resistance gene that has not been used previously for selection in mycobacteria. Tn4560, cloned in a Streptomyces plasmid, was introduced by electroporation into Mycobacterium smegmatis mc(2)155. Tn4560 transp...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bhatt A,Stewart GR,Kieser T

    更新日期:2002-01-10 00:00:00

  • Plasmid pC present in Salmonella enterica serovar Enteritidis PT14b strains encodes a restriction modification system.

    abstract::Salmonella enterica serovar Enteritidis (S. Enteritidis) possesses plasmids of different sizes and roles. Besides the serovar-specific virulence plasmid present in most field strains, S. Enteritidis can harbour plasmids of low molecular mass whose biological role is poorly understood. We therefore sequenced plasmid pC...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gregorova D,Pravcova M,Karpiskova R,Rychlik I

    更新日期:2002-09-10 00:00:00

  • Differential expression of putative drug resistance genes in Mycobacterium tuberculosis clinical isolates.

    abstract::Understanding drug resistance in Mycobacterium tuberculosis requires an integrated analysis of strain lineages, mutations and gene expression. Previously, we reported the differential expression of esxG, esxH, infA, groES, rpmI, rpsA and lipF genes in a sensitive M. tuberculosis strain and in a multidrug-resistant cli...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: González-Escalante L,Peñuelas-Urquides K,Said-Fernández S,Silva-Ramírez B,Bermúdez de León M

    更新日期:2015-12-01 00:00:00

  • Aberrant gene for E1 Tor hemolysin from Vibrio cholerae non-O1, N037.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Honma Y,Yamamoto K,Iwanaga M

    更新日期:1995-11-01 00:00:00

  • Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals.

    abstract::Nine clinical isolates of Enterobacteriaceae (six Escherichia coli and three Proteus mirabilis) isolated in three Parisian hospitals between 1989 and 2000 showed a particular extended-spectrum cephalosporin-resistance profile characterized by resistance to cefotaxime and aztreonam but not to ceftazidime. CTX-M-1, CTX-...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Saladin M,Cao VT,Lambert T,Donay JL,Herrmann JL,Ould-Hocine Z,Verdet C,Delisle F,Philippon A,Arlet G

    更新日期:2002-04-09 00:00:00

  • Differentiation of enterococci from other group D streptococci by means of a specific monoclonal antibody.

    abstract::We report on the specificity of a monoclonal antibody which reacts with autoclaved extracts of four species of enterococci but does not react to the same extent with similar extracts from two non-enterococcal group D streptococci. The monoclonal antibody also reacts specifically with purified lipoteichoic acid from St...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hammonds SJ,Easterbrook TJ,McIllmurray MB

    更新日期:1991-07-15 00:00:00

  • Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker.

    abstract::The sacB gene of Bacillus subtilis was successfully applied in various Arthrobacter, Brevibacterium, Corynebacterium and Rhodococcus strains for the isolation of transposable elements. Three different insertion sequence (IS) elements entrapped in sacB were isolated. The IS elements IS-Bl and IS-Cg isolated from Brevib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jäger W,Schäfer A,Kalinowski J,Pühler A

    更新日期:1995-02-01 00:00:00

  • Siro(haem)amide in Allochromatium vinosum and relevance of DsrL and DsrN, a homolog of cobyrinic acid a,c-diamide synthase, for sulphur oxidation.

    abstract::In the purple sulphur bacterium Allochromatium vinosum, the prosthetic group of dissimilatory sulphite reductase (DsrAB) was identified as siroamide, an amidated form of the classical sirohaem. The genes dsrAB are the first two of a large cluster of genes necessary for the oxidation of sulphur globules stored intracel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lübbe YJ,Youn HS,Timkovich R,Dahl C

    更新日期:2006-08-01 00:00:00

  • Molecular characterization of the erythromycin resistance plasmid pPV142 from Staphylococcus simulans.

    abstract::The 2.5-kb erythromycin resistance (EmR) plasmid pPV142 of Staphylococcus simulans 13044 was isolated and characterized. Sequence analysis identified ORF1 and ORF2 encoding a 158-residue replication protein (Rep142) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransferase), respective...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Somkuti GA,Solaiman DK,Steinberg DH

    更新日期:1998-08-15 00:00:00

  • ABC transporters in antibiotic-producing actinomycetes.

    abstract::Many antibiotic-producing actinomycetes possess at least one ABC (ATP-binding cassette) transporter which forms part of the antibiotic biosynthetic pathway and in most cases confers resistance to the drug in an heterologous host. Three types of antibiotic ABC transporters have been so far described in producer organis...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Méndez C,Salas JA

    更新日期:1998-01-01 00:00:00

  • Molecular cloning of a light-inducible gene (lipA) encoding a novel pilin from Arthrobacter photogonimos.

    abstract::Development of pili on cells of Arthrobacter photogonimos is induced by photo-oxidative conditions. The nucleotide sequence was determined of a light-inducible gene (lipA) that encodes the precursor of a light-inducible pilin (designated LIP), a polypeptide of 212 amino acids. The N-terminal leader peptide includes a ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yang HS,Hoober JK

    更新日期:1998-06-01 00:00:00

  • Attempts to revive Mycobacterium tuberculosis from 300-year-old human mummies.

    abstract::Environmental persistence of Mycobacterium tuberculosis is subject to speculation. However, the reality that infected postmortem tissues can be a danger to pathologists and embalmers has worrisome implications. A few experimental studies have demonstrated the organism's ability to withstand exposure to embalming fluid...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lemma E,Zimhony O,Greenblatt CL,Koltunov V,Zylber MI,Vernon K,Spigelman M

    更新日期:2008-06-01 00:00:00

  • Isolation and characterization of bacteriophages infecting Salmonella spp.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Carey-Smith GV,Billington C,Cornelius AJ,Hudson JA,Heinemann JA

    更新日期:2006-05-01 00:00:00

  • Biodegradation of phenanthrene by Alcaligenes sp. strain PPH: partial purification and characterization of 1-hydroxy-2-naphthoic acid hydroxylase.

    abstract::Alcaligenes sp. strain PPH degrades phenanthrene via 1-hydroxy-2-naphthoic acid (1-H2NA), 1,2-dihydroxynaphthalene (1,2-DHN), salicylic acid and catechol. Enzyme activity versus growth profile and heat stability studies suggested the presence of two distinct hydroxylases, namely 1-hydroxy-2-naphthoic acid hydroxylase ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Deveryshetty J,Phale PS

    更新日期:2010-10-01 00:00:00

  • Actinobacillus pleuropneumoniae does not require urease activity to produce acute swine pleuropneumonia.

    abstract::The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease-negative mutant was isolated following transposon mutagenesis with a mini-Tn10 derivative. Both the parent strain and the urease-negative mutant exhibited identical LD50 values in a murine infection model. Pig challenge...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tascón Cabrero RI,Vázquez-Boland JA,Gutiérrez CB,Rodríguez-Barbosa JI,Rodríguez-Ferri EF

    更新日期:1997-03-01 00:00:00

  • Correlation between cell-associated mannose-sensitive hemagglutination by Vibrio parahaemolyticus and adherence to a human colonic cell line Caco-2.

    abstract::Cell-associated hemagglutination (cHA) activity with human erythrocytes was examined for 468 clinical and 71 environmental strains of Vibrio parahaemolyticus. Approximately 95% of the strains tested were cHA positive irrespective of source or Kanagawa phenomenon. 75% of clinical strains showed relatively strong mannos...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nagayama K,Oguchi T,Arita M,Honda T

    更新日期:1994-07-01 00:00:00

  • Biotransformation of trinitrotoluene by Citrobacter sp. YC4 and evaluation of its cyto-toxicological effects.

    abstract::Trinitrotoluene (TNT) is an explosive chemical generally used for military, civil and industrial purposes. Therefore, TNT residue can be found in soil and ground water as an environmental pollutant. The environmental control of TNT pollution has become a critical issue because of its potential toxicity and carcinogeni...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kao CM,Wei SF,Chen SC,Yao CL,Ma C,Chien CC

    更新日期:2018-01-01 00:00:00

  • The Moraxella catarrhalis outer membrane protein CD contains two distinct domains specifying adherence to human lung cells.

    abstract::Most Moraxella catarrhalis isolates express a highly-conserved outer membrane protein of 453 residues designated OMPCD, which has been previously shown to mediate binding to A549 human lung cells. Here, it is reported that two distinct domains of the M. catarrhalis strain O35E OMPCD protein specify adherence. Truncate...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Akimana C,Lafontaine ER

    更新日期:2007-06-01 00:00:00

  • The expression of the flagellum of Legionella pneumophila is modulated by different environmental factors.

    abstract::Legionella pneumophila, the aetiologic agent of legionnaires' disease, contains a single, monopolar flagellum which is composed of one major subunit, the FlaA protein. Expression studies using a reporter gene fusion of the flaA promoter with the luxAB gene revealed that the flaA expression is not only temperature regu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Heuner K,Brand BC,Hacker J

    更新日期:1999-06-01 00:00:00

  • Characterization of the eaeA gene from rabbit enteropathogenic Escherichia coli strain RDEC-1 and comparison to other eaeA genes from bacteria that cause attaching-effacing lesions.

    abstract::A number of enteric pathogens, including enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli, Hafnia alvei, a strain of Citrobacter freundii, and rabbit EPEC strain RDEC-1 cause attaching-effacing (AE) lesions in the gut mucosa. These bacteria have a pathogenicity cassette (locus of enterocyte efface...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Agin TS,Cantey JR,Boedeker EC,Wolf MK

    更新日期:1996-11-01 00:00:00

  • Inactivation of the petE gene encoding plastocyanin causes different photosynthetic responses in cyanobacterium Synechocystis PCC 6803 under light-dark photoperiod and continuous light conditions.

    abstract::Plastocyanin, encoded by the petE gene, can transfer electrons to photosystem I (PSI) and cytochrome c oxidase during photosynthetic and respiratory metabolism in cyanobacteria. We constructed a petE mutant of Synechocystis sp. strain PCC 6803 and investigated its phenotypic properties under different light conditions...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Wang XQ,Jiang HB,Zhang R,Qiu BS

    更新日期:2013-04-01 00:00:00

  • Fluorescence in situ hybridisation (FISH)--the next generation.

    abstract::Fluorescence in situ hybridisation (FISH) has become one of the major techniques in environmental microbiology. The original version of this technique often suffered from limited sensitivity due to low target copy number or target inaccessibility. In recent years there have been several developments to amend this prob...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Zwirglmaier K

    更新日期:2005-05-15 00:00:00

  • Isolation and identification of nematode-antagonistic compounds from the fungus Aspergillus candidus.

    abstract::Culture medium from an isolate of the fungus Aspergillus candidus was extracted, fractionated and examined to discover compounds antagonistic to plant-parasitic nematodes that are important pathogens of agricultural crops. Column, thin layer and preparative chromatographies and spectral and elemental analyses, were us...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shemshura ON,Bekmakhanova NE,Mazunina MN,Meyer SL,Rice CP,Masler EP

    更新日期:2016-03-01 00:00:00

  • Environmental pH affects transcriptional responses to cadmium toxicity in Escherichia coli K-12 (MG1655).

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Worden CR,Kovac WK,Dorn LA,Sandrin TR

    更新日期:2009-04-01 00:00:00

  • Giardia lamblia -- a model organism for eukaryotic cell differentiation.

    abstract::Giardia lamblia is a binucleated, flagellated protozoan parasite that inhabits the upper small intestine of its vertebrate hosts. The entire life cycle, which can be completed in vitro, is simple with cycling between a vegetative trophozoite and a highly resistant cystic form. The parasite is one of the earliest diver...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Svärd SG,Hagblom P,Palm JE

    更新日期:2003-01-21 00:00:00

  • Flow cytometric analysis of prolonged stress-dependent heterogeneity in bacterial cells.

    abstract::When bacterial cells are subjected to stress, cellular and population characteristics can change significantly. Rapid acquisition of these properties of cells in their native physiological state is necessary in order to precisely interpret their survival modes. The multiparametric analytical capability of flow cytomet...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sachidanandham R,Gin KY

    更新日期:2009-01-01 00:00:00

  • Lactobacillus reuteri A9 and Lactobacillus mucosae A13 isolated from Chinese superlongevity people modulate lipid metabolism in a hypercholesterolemia rat model.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jiang J,Feng N,Zhang C,Liu F,Zhao J,Zhang H,Zhai Q,Chen W

    更新日期:2019-12-01 00:00:00

  • Extracellular polymeric substances responsible for bacterial adhesion onto solid surface.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tsuneda S,Aikawa H,Hayashi H,Yuasa A,Hirata A

    更新日期:2003-06-27 00:00:00