Activity of the enzymes involved in the synthesis of exopolysaccharide precursors in an overproducing mutant ropy strain of Streptococcus thermophilus.

Abstract:

:The activities of some enzymes belonging to the Leloir pathway, phosphoglucomutase, UDP-glucose pyrophosphorylase, UDP-galactose 4-epimerase and galactose 1-P uridyl transferase, were studied in a wild ropy, a non-ropy and an overproducing mutant ropy strain of Streptococcus thermophilus. These activities were assayed over successive culture transfers along with exocellular polysaccharide (EPS) production. The overproducing mutant ropy strain showed increments in polysaccharide production over successive culture transfers, as opposed to reductions in production by the wild ropy strain. The observed variations among strains in the enzyme activities that were analysed in relation to EPS production suggest their involvement in the synthesis of sugar-nucleotide EPS precursors.

journal_name

FEMS Microbiol Lett

authors

Escalante A,Villegas J,Wacher C,García-Garibay M,Farrés A

doi

10.1111/j.1574-6968.2002.tb11146.x

keywords:

subject

Has Abstract

pub_date

2002-04-09 00:00:00

pages

289-93

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109702005621

journal_volume

209

pub_type

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