Induction of L-phenylalanine ammonia-lyase and suppression of veratryl alcohol biosynthesis by exogenously added L-phenylalanine in a white-rot fungus phanerochaete chrysosporium

Abstract:

:The effects of exogenously added L-phenylalanine (L-Phe) on the activities of L-phenylalanine ammonia-lyase (PAL) and 3, 4-dimethoxybenzyl alcohol (veratryl alcohol, VA) biosynthesis in ligninolytic cultures of Phanerochaete chrysosporium were investigated. Increasing PAL activity was detected in low nitrogen (LN) culture but not in high nitrogen (HN) culture. The addition of L-Phe into the LN culture caused a 25-fold increase in enzyme activity, which clearly shows that L-Phe, a substrate of the enzyme, served as an inducer of PAL. The increase in activity of PAL triggered by nitrogen starvation was correlated with biosynthesis of VA. However, PAL induced by the added L-Phe did not promote VA biosynthesis but suppressed the biosynthesis probably due to NH(4)(+) released from L-Phe.

journal_name

FEMS Microbiol Lett

authors

Hattori T,Nishiyama A,Shimada M

doi

10.1111/j.1574-6968.1999.tb08742.x

keywords:

subject

Has Abstract

pub_date

1999-10-15 00:00:00

pages

305-9

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109799004279

journal_volume

179

pub_type

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