Abstract:
:Two Csp proteins (CspA and CspD) were fused to the green fluorescent protein GFP and expressed from their natural promoters or from an inducible promoter. Fluorescence microscopy and computerized image analysis indicate that in Escherichia coli growing at 37 degrees C CspD localizes in the nucleoid like the control H-NS while CspA occupies a polar position away from the nucleoid. Following cold shock CspA maintains its location, while CspD is not sufficiently expressed to permit its localization. The different localization of CspA and CspD indicates that these proteins play different roles in the cell in spite of their extensive structural similarity.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Giangrossi M,Exley RM,Le Hegarat F,Pon CLdoi
10.1111/j.1574-6968.2001.tb10799.xkeywords:
subject
Has Abstractpub_date
2001-08-21 00:00:00pages
171-6issue
2eissn
0378-1097issn
1574-6968pii
S0378109701002919journal_volume
202pub_type
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