Different in vivo localization of the Escherichia coli proteins CspD and CspA.

Abstract:

:Two Csp proteins (CspA and CspD) were fused to the green fluorescent protein GFP and expressed from their natural promoters or from an inducible promoter. Fluorescence microscopy and computerized image analysis indicate that in Escherichia coli growing at 37 degrees C CspD localizes in the nucleoid like the control H-NS while CspA occupies a polar position away from the nucleoid. Following cold shock CspA maintains its location, while CspD is not sufficiently expressed to permit its localization. The different localization of CspA and CspD indicates that these proteins play different roles in the cell in spite of their extensive structural similarity.

journal_name

FEMS Microbiol Lett

authors

Giangrossi M,Exley RM,Le Hegarat F,Pon CL

doi

10.1111/j.1574-6968.2001.tb10799.x

keywords:

subject

Has Abstract

pub_date

2001-08-21 00:00:00

pages

171-6

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109701002919

journal_volume

202

pub_type

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