Regulation of beta-glucosidase biosynthesis in Aspergillus nidulans.

abstract：:We have constructed a very efficient synthesis and secretion system for cholera toxin B subunit (CTB) of Vibrio cholerae 569B using Bacillus-brevis. The constructed expression-secretion vector has the multiple promoters and the signal peptide coding region of the mwp gene, a structural gene for one of the major cell w...

journal_title：FEMS microbiology letters

authors： Ichikawa Y,Yamagata H,Tochikubo K,Udaka S

更新日期：1993-08-01 00:00:00

abstract：:Legionella pneumophila, the aetiologic agent of legionnaires' disease, contains a single, monopolar flagellum which is composed of one major subunit, the FlaA protein. Expression studies using a reporter gene fusion of the flaA promoter with the luxAB gene revealed that the flaA expression is not only temperature regu...

journal_title：FEMS microbiology letters

authors： Heuner K,Brand BC,Hacker J

更新日期：1999-06-01 00:00:00

abstract：:Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall-degra...

journal_title：FEMS microbiology letters

authors： Debeire P,Delalande F,Habrylo O,Jeltsch JM,Van Dorsselaer A,Phalip V

更新日期：2014-06-01 00:00:00

abstract：:The ADP-ribosylation factor (ARF) family of GTPases are highly conserved from yeast to human and regulate vesicle budding. Sec7 domain containing proteins stimulate the guanine nucleotide exchange on Arf proteins, while ARF-GTPase activating proteins stimulate the hydrolysis of GTP. Since vesicle trafficking is import...

journal_title：FEMS microbiology letters

authors： Oscarsson T,Walther A,Lengeler KB,Wendland J

更新日期：2017-12-29 00:00:00

abstract：:The genes for fructosyltransferase (ftf) and glucan-binding protein (gbp) of Streptococcus mutans strain Ingbritt have been cloned in E. coli using bacteriophage and plasmid vectors. A single ftf gene appears to be responsible for the appearance of several FTF of different electrophoretic mobilities. Despite the fact ...

journal_title：FEMS microbiology letters

authors： Aduse-Opoku J,Gilpin ML,Russell RR

更新日期：1989-06-01 00:00:00

abstract：:Attachment of soil bacteria to plant cells is supposedly the very early step required in plant-microbe interactions. Attachment also is an initial step for the formation of microbial biofilms on plant roots. For the rhizobia-legume symbiosis, various mechanisms and diverse surface molecules of both partners have been ...

journal_title：FEMS microbiology letters

authors： Rodríguez-Navarro DN,Dardanelli MS,Ruíz-Saínz JE

更新日期：2007-07-01 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) is a key enzyme in cellular one-carbon pathway and has been studied in many living organisms from bacteria to higher plants and mammals. However, biochemical and molecular characterization of SHMT from photoautotrophic microorganisms remains a challenge. Here, we isolated the SHM...

journal_title：FEMS microbiology letters

authors： Waditee-Sirisattha R,Sittipol D,Tanaka Y,Takabe T

更新日期：2012-08-01 00:00:00

abstract：:Prevotella ruminicola B1(4) is a strictly anaerobic, Gram-negative, polysaccharide-degrading rumen bacterium. Xylanase activity in this strain was found to be inducible, the specific activity of cells grown on xylan being increased at least 20-fold by comparison with cells grown on glucose. Ten bacteriophage clones ex...

journal_title：FEMS microbiology letters

authors： Gasparic A,Marinsek-Logar R,Martin J,Wallace RJ,Nekrep FV,Flint HJ

更新日期：1995-01-15 00:00:00

abstract：:In this study, internal size variations in the VanA gene cluster Tn1546, encoding resistance to glycopeptides, is described. Studies of previously uncharacterized size variations of an internal region, encoding the vanX and vanY genes of Tn1546, revealed that these variations were due to the presence of the IS sequenc...

journal_title：FEMS microbiology letters

authors： Jensen LB

更新日期：1998-12-15 00:00:00

abstract：:The large intestine of dogs contains a complex microbial ecosystem with predominance of streptococci, bifidobacteria, lactobacilli, Bacteroides and Clostridium. Generally, this predominant microbiota in dogs is relatively stable in time but much less is known about its taxonomic composition. Moreover, almost no studie...

journal_title：FEMS microbiology letters

authors： Vanhoutte T,Huys G,De Brandt E,Fahey GC Jr,Swings J

更新日期：2005-08-01 00:00:00

abstract：:The fatty acid composition during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes (metacyclogenesis) was analysed by gas-liquid chromatography and mass spectrometry. Significant qualitative and quantitative changes in the fatty acid composition occurred during incubation of epimas...

journal_title：FEMS microbiology letters

authors： Esteves MG,Gonzales-Perdomo M,Alviano CS,Angluster J,Goldenberg S

更新日期：1989-05-01 00:00:00

abstract：:Yeast two-hybrid and Far Western analyses were used to detect interactions between Bacillus subtilis spores' nutrient germinant receptor proteins and proteins encoded by the spoVA operon, all of which are involved in spore germination and located in the spores' inner membrane. These analyses indicated that two subunit...

journal_title：FEMS microbiology letters

authors： Vepachedu VR,Setlow P

更新日期：2007-09-01 00:00:00

abstract：:Growth of Prevotella ruminicola strains B(1)4 (subsp. brevis) and D31d (subsp. ruminicola), was inhibited by protamine, a polycationic, low molecular mass protein. Results showed that protamine has a bacteriocidal effect when present in concentrations exceeding 30 micrograms ml-1. Protamine exerted its toxic effects b...

journal_title：FEMS microbiology letters

authors： Madeira HM,Morrison M

更新日期：1997-05-01 00:00:00

abstract：:Inoculation of soil with bacteria (a Gram-negative rod [PD2] and a 4-membered consortium [DC1]) accelerated mineralisation of phenanthrene and pyrene (but not naphthalene) added individually to a pristine sand and a pristine organic soil. The half-life of naphthalene was 3.5 days in both soils whether inoculated or no...

journal_title：FEMS microbiology letters

authors： Smith MJ,Lethbridge G,Burns RG

更新日期：1997-07-01 00:00:00

abstract：:Two clones, obtained by polymerase chain reaction from macronuclear DNA of the unicellular ciliated protist Blepharisma japonicum, were isolated and sequenced. They correspond to fragments of two different putative H4 histone genes. The existence of multiple H4 histone genes was also suggested by Southern blot hybridi...

journal_title：FEMS microbiology letters

authors： Salvini M,Bini E,Santucci A,Batistoni R

更新日期：1997-04-01 00:00:00

abstract：:Volvariella volvacea, strain V14, produces multiple forms of extracellular laccase when grown in submerged culture in a defined medium with glucose as sole carbon source, and on cotton waste 'compost' representative of the conditions used for industrial-scale mushroom cultivation. In liquid culture, enzyme synthesis i...

journal_title：FEMS microbiology letters

authors： Chen S,Ma D,Ge W,Buswell JA

更新日期：2003-01-21 00:00:00

abstract：:Escherichia coli heat-labile enterotoxin B subunit (EtxB) has been proposed as a potential protein carrier for the delivery of heterologous peptides to target cells, particularly for the oral delivery of epitopes to the mucosal immune system. In this study, two extensions to the C-terminus of EtxB were genetically eng...

journal_title：FEMS microbiology letters

authors： Marcello A,Loregian A,Palù G,Hirst TR

更新日期：1994-03-15 00:00:00

abstract：:There are no published methods on plasmid isolation from Peptostreptococcus spp., therefore two methods of plasmid isolation from this genera were analysed: the boiling and alkaline-SDS methods. Plasmid DNA was not recovered by the boiling method, however, with the alkaline-SDS method, cryptic plasmid DNA was detected...

journal_title：FEMS microbiology letters

authors： Ng LK,Dillon JA

更新日期：1989-10-01 00:00:00

abstract：:Bacteriophages infecting Salmonella spp. were isolated from sewage using soft agar overlays containing three Salmonella serovars and assessed with regard to their potential to control food-borne salmonellae. Two distinct phages, as defined by plaque morphology, structure and host range, were obtained from a single sam...

journal_title：FEMS microbiology letters

authors： Carey-Smith GV,Billington C,Cornelius AJ,Hudson JA,Heinemann JA

更新日期：2006-05-01 00:00:00

abstract：:In artificial engineered systems, nitrification is a key reaction that accounts for the removal of biological nitrogen. Recently, a single microbe capable of oxidizing ammonia to nitrate, known as a complete ammonia oxidizer (comammox), has been discovered. Although the abundance and diversity of comammox Nitrospira i...

journal_title：FEMS microbiology letters

authors： Fujitani H,Nomachi M,Takahashi Y,Hasebe Y,Eguchi M,Tsuneda S

更新日期：2020-01-01 00:00:00

abstract：:In this multidisciplinary study, we combined morphological, physiological, and phylogenetic approaches to identify three dominant water bloom-forming Cyanobacteria in a tropical marine mangrove in Guadeloupe (French West Indies). Phylogenetic analysis based on 16S rRNA gene sequences place these marine Cyanobacteria i...

journal_title：FEMS microbiology letters

authors： Guidi-Rontani C,Jean MR,Gonzalez-Rizzo S,Bolte-Kluge S,Gros O

更新日期：2014-10-01 00:00:00

abstract：:The synthesis of inducible biodegradative threonine dehydratase of Escherichia coli increased several-fold in the presence of the DNA gyrase inhibitors, nalidixic acid and coumermycin. Temperature-sensitive gyrB mutants expressed higher levels of dehydratase as compared to an isogenic gyrB+ strain. Immunoblotting expe...

journal_title：FEMS microbiology letters

authors： Sumantran VN,Tranguch AJ,Datta P

更新日期：1989-11-01 00:00:00

abstract：:When the amino acid-fermenting bacterium Clostridium aminophilum F was inoculated into media containing 1 microM monensin or a bacteriocin-like inhibitory substance (BLIS) from Butyrivibrio fibrisolvens JL5, the cultures lagged and growth was not observed for more than 12 h. The monensin- and BLIS-treated cultures eve...

journal_title：FEMS microbiology letters

authors： Rychlik JL,Russell JB

更新日期：2002-03-19 00:00:00

abstract：:A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained m...

journal_title：FEMS microbiology letters

authors： Law C,Kavanagh K,Whittaker P

更新日期：1994-01-01 00:00:00

abstract：:Multiple restriction fragments, homologous to the previously described Mycoplasma bovis vspA gene, were identified in the chromosome of Mycoplasma agalactiae. The vspA, a representative variable surface lipoprotein gene of the vsp gene family, and four synthetic oligonucleotides, representing sequences complementary t...

journal_title：FEMS microbiology letters

authors： Flitman-Tene R,Levisohn S,Rosenbusch R,Rapoport E,Yogev D

更新日期：1997-11-01 00:00:00

abstract：:In this study, 52 Streptococcus suis isolates from pigs in southern China were divided into four known sequence types (STs) and six new STs, using multilocus sequence typing. Ten representative isolates were selected from 10 STs for the analysis of whole genome sequences. Virulence was assessed in 10 isolates, which w...

journal_title：FEMS microbiology letters

authors： Peng L,Lin M,Huang Z,Guo S,Sun H,Yang X

更新日期：2020-03-01 00:00:00

abstract：:Expression of the dimethylsulfoxide respiratory (dor) operon of Rhodobacter is regulated by oxygen, light intensity and availability of substrate. Since dimethylsulfoxide reductase contains a pterin molybdenum cofactor, the role of molybdate in the regulation of dor operon expression was investigated. In this report w...

journal_title：FEMS microbiology letters

authors： Solomon PS,Shaw AL,Young MD,Leimkuhler S,Hanson GR,Klipp W,McEwan AG

更新日期：2000-09-15 00:00:00

abstract：:Brucella abortus B19, an avirulent strain obtained by spontaneous mutation, is used worldwide as a vaccine for the control of bovine brucellosis. B19 differs from other B. abortus strains in its sensitivity to erythritol. We took advantage of a previously obtained erythritol sensitive Tn5 insertion mutant of B. abortu...

journal_title：FEMS microbiology letters

authors： Sangari FJ,García-Lobo JM,Agüero J

更新日期：1994-09-01 00:00:00

abstract：:The hepA gene in Anabaena sp. strain PCC 7120 is required for normal formation of the polysaccharide layer of the heterocyst envelope. A plasmid bearing hepA, interrupted by a neomycin-resistance cassette, was transferred by conjugation to wild type Anabaena variabilis ATCC 29413, so that the interrupted hepA gene rep...

journal_title：FEMS microbiology letters

authors： Leganés F

更新日期：1994-10-15 00:00:00

abstract：:The best yield of lipopolysaccharide (LPS) of Pseudomonas pseudomallei GIFU 12046 was obtained by extraction of defatted cells by phenol/chloroform/petroleum ether. The LPS showed a smooth character on SDS-polyacrylamide gel electrophoresis and contained D-glucose, L-glycero-D-manno-heptose, and D-glucosamine as the m...

journal_title：FEMS microbiology letters

authors： Kawahara K,Dejsirilert S,Danbara H,Ezaki T

更新日期：1992-09-15 00:00:00