Abstract:
:Growth of Prevotella ruminicola strains B(1)4 (subsp. brevis) and D31d (subsp. ruminicola), was inhibited by protamine, a polycationic, low molecular mass protein. Results showed that protamine has a bacteriocidal effect when present in concentrations exceeding 30 micrograms ml-1. Protamine exerted its toxic effects by disrupting the outer membrane, which was demonstrated by: (i) an increased sensitivity to hydrophobic antibiotics (novobiocin and monensin) and (ii) release of the periplasmic enzyme alkaline phosphatase following short-term exposure to protamine. Although the concentrations of protamine inhibitory to P. ruminicola are relatively low, the effects of such a compound are probably too broad to permit its successful use in terms of manipulating ruminal proteolysis.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Madeira HM,Morrison Mdoi
10.1111/j.1574-6968.1997.tb10353.xsubject
Has Abstractpub_date
1997-05-01 00:00:00pages
81-8issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(97)00100-6journal_volume
150pub_type
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