Evaluating efficacy of bacteriophage therapy against Staphylococcus aureus infections using a silkworm larval infection model.

abstract：:During our screening of amylolytic microorganisms from Brazilian fruits, we isolated a yeast strain classified as Cryptococcus flavus. When grown on starch-containing medium this strain exhibited the highest amylase production after 24 h of cultivation. The extracellular amylase from C. flavus was purified from the cu...

journal_title：FEMS microbiology letters

authors： Wanderley KJ,Torres FA,Moraes LM,Ulhoa CJ

更新日期：2004-02-16 00:00:00

abstract：:The Gram-positive bacterium Streptococcus gordonii has been genetically engineered to allow the simultaneous expression of two heterologous proteins at the cell surface. A family of recombinant streptococci displaying two different antigens was constructed. All the strains were genetically stable and expressed both pr...

journal_title：FEMS microbiology letters

authors： Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR

更新日期：2002-04-23 00:00:00

abstract：:In sausage, Staphylococcus carnosus increases the levels of methyl ketones which could arise from incomplete beta-oxidation of fatty acids followed by a decarboxylation. The objective of this work was to characterize the beta-decarboxylase activities in cell-free extract. By using different substrates, at least two ki...

journal_title：FEMS microbiology letters

authors： Fadda S,Leroy-Sétrin S,Talon R

更新日期：2003-11-07 00:00:00

abstract：:A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 other bacterial species. A detection limit of 1 cell equivalent wa...

journal_title：FEMS microbiology letters

authors： O'Grady J,Lacey K,Glynn B,Smith TJ,Barry T,Maher M

更新日期：2009-12-01 00:00:00

abstract：:A novel 36-kDa endochitinase named chit36 has been isolated and characterized from Trichoderma harzianum Rifai TM. Partial amino acid sequences from the purified protein were used to clone the fungal cDNA, based on polymerase chain reaction with degenerate primers. The complete open reading frame encodes a 344-amino a...

journal_title：FEMS microbiology letters

authors： Viterbo A,Haran S,Friesem D,Ramot O,Chet I

更新日期：2001-06-25 00:00:00

abstract：:The FIS protein (factor for inversion stimulation) is known to activate the transcription of rRNA and tRNA operons in Escherichia coli. In this report the growth behaviour of a fis bacterium was studied under steady-state conditions and in competition experiments with a wild-type bacterium in a chemostat. The growth r...

journal_title：FEMS microbiology letters

authors： Nilsson L,Verbeek H,Hoffmann U,Haupt M,Bosch L

更新日期：1992-11-15 00:00:00

abstract：:Bacteriophages co-exist and co-evolve with their hosts in natural environments. Virulent phages lyse infected cells through lytic cycles, whereas temperate phages often remain dormant and can undergo lysogenic or lytic cycles. In their lysogenic state, prophages are actually part of the host genome and replicate passi...

journal_title：FEMS microbiology letters

authors： Menouni R,Hutinet G,Petit MA,Ansaldi M

更新日期：2015-01-01 00:00:00

abstract：:Fire blight, caused by plant pathogenic bacterium Erwinia amylovora, is one of the most important diseases of Rosaceae plants. Due to the lack of effective control measures, fire blight infections pose a recurrent threat on agricultural production worldwide. Recently, bacterial viruses, or bacteriophages, have been pr...

journal_title：FEMS microbiology letters

authors： Besarab NV,Akhremchuk AE,Zlatohurska MA,Romaniuk LV,Valentovich LN,Tovkach FI,Lagonenko AL,Evtushenkov AN

更新日期：2020-05-01 00:00:00

abstract：:To elucidate the molecular mechanism by which the MexR repressor regulates expression of the MexAB-OprM efflux pump, we investigated MexR and the mexR-mexA intergenic DNA (mexOP) interaction, and transcription of the mexA-lacZ reporter gene containing different lengths of mexOP. Homogeneously purified MexR bound speci...

journal_title：FEMS microbiology letters

authors： Saito K,Eda S,Maseda H,Nakae T

更新日期：2001-02-05 00:00:00

abstract：:Xanthomonas oryzae pv. oryzae is an important plant pathogen which causes bacterial blight of rice. To facilitate genome studies of this bacterium, we have constructed a bacterial artificial chromosome (BAC) library of strain MAFF 311018. It consisted of 750 clones representing 16 genome equivalents, and had an insert...

journal_title：FEMS microbiology letters

authors： Ochiai H,Inoue Y,Hasebe A,Kaku H

更新日期：2001-06-12 00:00:00

abstract：:Marine ammonia oxidising bacteria were enriched by incubation of sea water, amended with ammonium sulphate, and subsequent subculture in liquid inorganic medium. PCR primers were designed to be specific for rDNA sequences from ammonia oxidisers belonging to the beta-sub-group of the proteobacteria. These primers were ...

journal_title：FEMS microbiology letters

authors： McCaig AE,Embley TM,Prosser JI

更新日期：1994-07-15 00:00:00

abstract：:Control of Helminthosporium solani, the cause of silver scurf in potato tubers, has been impaired by selection of benzimidazole-resistant strains as a result of repeated use of the fungicide thiabendazole. Identification of thiabendazole-resistant strains of H. solani by conventional techniques takes several weeks. Pr...

journal_title：FEMS microbiology letters

authors： McKay GJ,Cooke LR

更新日期：1997-07-15 00:00:00

abstract：:A Na(+)/H(+) antiporter gene was isolated from alkaliphilic Bacillus sp. G1. The full-length sequence of the Na(+)/H(+) antiporter gene was obtained using a genome walking method, and designated as g1-nhaC. An ORF preceded by a promoter-like sequence and a Shine-Dalgarno sequence, and followed by a terminator-like seq...

journal_title：FEMS microbiology letters

authors： Liew CW,Illias RM,Mahadi NM,Najimudin N

更新日期：2007-11-01 00:00:00

abstract：:Competition assays measure differences between populations of bacteria after stress adaptation, populations of different bacteria and mutations in antibiotic resistance genes. We have developed a competition-based assay to evaluate if genes upregulated under starvation are important for bacterial survival. Stress resp...

journal_title：FEMS microbiology letters

authors： Havis S,Rangel J,Mali S,Bodunrin A,Housammy Z,Zimmerer R,Murphy J,Widger WR,Bark SJ

更新日期：2019-03-01 00:00:00

abstract：:Proteins which are secreted or associated with the cell envelope of Mycobacterium tuberculosis may contain protective T-cell epitopes. Prior to this study, a recombinant clone bank of enzymatically active M. tuberculosis-alkaline phosphatase fusions, were screened for immunogenicity in a murine T-cell model. Five of t...

journal_title：FEMS microbiology letters

authors： Moran AJ,Doran JL,Wu J,Treit JD,Ekpo P,Kerr VJ,Roberts AD,Orme IM,Galant S,Ress SR,Nano FE

更新日期：1999-08-01 00:00:00

abstract：:Pseudomonas fragi is a meat and milk spoilage bacterium with high iron requirements; however, mechanisms of iron acquisition remain largely unknown. The aim of this work was to investigate siderophore production as an iron acquisition system for P. fragi. A vibrioferrin siderophore gene cluster was identified in 13 P....

journal_title：FEMS microbiology letters

authors： Stanborough T,Fegan N,Powell SM,Tamplin M,Chandry PS

更新日期：2018-03-01 00:00:00

abstract：:The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) ...

journal_title：FEMS microbiology letters

authors： Edwards K,Linetsky I,Hueser C,Eisenstark A

更新日期：2001-05-30 00:00:00

abstract：:The Chaetomium globosum strain F0142, which was isolated from barnyard grass, showed potent disease control efficacy against rice blast (Magnaporthe grisea) and wheat leaf rust (Puccinia recondita). Two antifungal substances were purified from broth from this organism and identified as chaetoviridins A and B. Chaetovi...

journal_title：FEMS microbiology letters

authors： Park JH,Choi GJ,Jang KS,Lim HK,Kim HT,Cho KY,Kim JC

更新日期：2005-11-15 00:00:00

abstract：:A Tn5 transposon mutant was isolated of the alkylphenol degrader Pseudomonas sp. KL28 that showed increased growth at higher levels of m-cresol on solid and in liquid cultures. The transposon was inserted at the 5'-terminus of rpoS, which encodes a stationary-phase sigma factor. When grown on agar plates, the rpoS mut...

journal_title：FEMS microbiology letters

authors： Yun JI,Cho KM,Kim JK,Lee SO,Cho K,Lee K

更新日期：2007-04-01 00:00:00

abstract：:Pythium drechsleri Paul was described as a new species from soil samples taken in a salt-marsh of Arzew, Algeria [Paul, B. (1988) Une nouvelle espèce de Pythium isolée d'une saline de l'ouest Algérien. Cryptogam. Mycol. 9, 325-333]. The name of the fungus, P. drechsleri, is a nomen invalidum, as it is a later homonym ...

journal_title：FEMS microbiology letters

authors： Paul B

更新日期：2000-02-01 00:00:00

abstract：:The hepA gene in Anabaena sp. strain PCC 7120 is required for normal formation of the polysaccharide layer of the heterocyst envelope. A plasmid bearing hepA, interrupted by a neomycin-resistance cassette, was transferred by conjugation to wild type Anabaena variabilis ATCC 29413, so that the interrupted hepA gene rep...

journal_title：FEMS microbiology letters

authors： Leganés F

更新日期：1994-10-15 00:00:00

abstract：:A two-step enzymatic synthesis process of 4-hydroxyisoleucine is suggested. In the first step, the aldol condensation of acetaldehyde and alpha-ketobutyrate catalyzed by specific aldolase results in the formation of 4-hydroxy-3-methyl-2-keto-pentanoate (HMKP). In the second step, amination of HMKP by the branched-chai...

journal_title：FEMS microbiology letters

authors： Smirnov SV,Samsonova NN,Novikova AE,Matrosov NG,Rushkevich NY,Kodera T,Ogawa J,Yamanaka H,Shimizu S

更新日期：2007-08-01 00:00:00

abstract：:Bacillus subtilis possesses two isogenes encoding glutamate racemases, the poly-gamma-glutamate synthesis-linking Glr enzyme and the YrpC isozyme, and produces abundant amounts of the Glr enzyme. The YrpC isozyme, but not the Glr enzyme, was found to influence the activity of DNA gyrase, as did the MurI-type glutamate...

journal_title：FEMS microbiology letters

authors： Ashiuchi M,Kuwana E,Komatsu K,Soda K,Misono H

更新日期：2003-06-27 00:00:00

abstract：:Bacteriophage M102 is a virulent siphophage that propagates in some serotype c Streptococcus mutans strains, but not in S. mutans of serotype e, f or k. The serotype of S. mutans is determined by the glucose side chain of rhamnose-glucose polysaccharide (RGP). Because the first step in the bacteriophage infection proc...

journal_title：FEMS microbiology letters

authors： Shibata Y,Yamashita Y,van der Ploeg JR

更新日期：2009-05-01 00:00:00

abstract：:We have constructed a fungal subtractive suppressive library enriched in genes expressed during appressorium maturation in Magnaporthe grisea. Sequencing of 250 clones from the subtracted appressorium cDNA library revealed 142 unique genes, represented by 155 non-redundant ESTs (expressed sequence tags). Of these ESTs...

journal_title：FEMS microbiology letters

authors： Lu JP,Liu TB,Lin FC

更新日期：2005-04-01 00:00:00

abstract：:Effects of the two-component sensor-regulator pairs DctBD and NtrBC upon the expression of a dctA::phoA fusion from Rhizobium meliloti were determined under excess and limiting nitrogen concentrations in Escherichia coli. Results indicated that NtrBC affected transcription from the dctA promoter on a number of regulat...

journal_title：FEMS microbiology letters

authors： Allaway D,Boesten B,O'Gara F

更新日期：1995-05-15 00:00:00

abstract：:Digital game-based learning (DGBL) is being used increasingly as an alternative learning tool to teach science in further and higher education. A variety of digital game formats currently exist for science learning, alongside diverse methods for their implementation and evaluation. This paper aims to provide a broad s...

journal_title：FEMS microbiology letters

authors： Brown CL,Comunale MA,Wigdahl B,Urdaneta-Hartmann S

更新日期：2018-11-01 00:00:00

abstract：:Multidrug resistance plasmids carrying the bla(CMY-2) gene have been identified in Salmonella enterica serovars Typhimurium and Newport from the United States. This gene confers decreased susceptibility to ceftriaxone, and is most often found in strains with concomitant resistance to ampicillin, chloramphenicol, strep...

journal_title：FEMS microbiology letters

authors： Doublet B,Carattoli A,Whichard JM,White DG,Baucheron S,Chaslus-Dancla E,Cloeckaert A

更新日期：2004-04-15 00:00:00

abstract：:Fusobacterium nucleatum is a common oral anaerobe associated with gingivitis, periodontal disease and preterm deliveries. Coaggregation among oral bacteria is considered to be a significant factor in dental plaque development. Adhesion to host cells was suggested to be important for the F. nucleatum virulence associat...

journal_title：FEMS microbiology letters

authors： Bachrach G,Ianculovici C,Naor R,Weiss EI

更新日期：2005-07-15 00:00:00

abstract：:Ninety-three Salmonella isolates recovered from commercial foods and exotic animals in Colombia were studied. The serotypes, resistance profiles and where applicable the quinolone resistance genes were determined. Salmonella Anatum (n=14), Uganda (19), Braenderup (10) and Newport (10) were the most prevalent serovars,...

journal_title：FEMS microbiology letters

authors： Karczmarczyk M,Martins M,McCusker M,Mattar S,Amaral L,Leonard N,Aarestrup FM,Fanning S

更新日期：2010-12-01 00:00:00