Identification of novel immunogenic Mycobacterium tuberculosis peptides that stimulate mononuclear cells from immune donors.

abstract：:We developed a multiplex PCR to detect the presence of methicillin- (mecA), cadmium/zinc-(czrC) and antiseptic-resistant (qacA/B) staphylococci and to identify Panton-Valentine leukocidin (PVL)-positive and -negative Staphylococcus aureus and coagulase-negative staphylococci (CoNS) from infected and healthy eyes. The ...

journal_title：FEMS microbiology letters

authors： Panda S,Kar S,Choudhury R,Sharma S,Singh DV

更新日期：2014-03-01 00:00:00

abstract：:In a previous study, we isolated the spaA gene encoding the surface protective antigen A, SpaA, of Erysipelothrix rhusiopathiae, and found that the N-terminal region of SpaA was responsible for protective immunity against erysipelas and that the C-terminal region contained eight repeat units consisting of 20 amino aci...

journal_title：FEMS microbiology letters

authors： Makino SI,Yamamoto K,Asakura H,Shirahata T

更新日期：2000-05-15 00:00:00

abstract：:Many bacteria are capable of accumulating intracellular granules of polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis of bacterial cells to study changes in the diameters of cells as well as PHA granules during growth and PHA accumulation in the bacterium Cupriavidus necator H16 (for...

journal_title：FEMS microbiology letters

authors： Mravec F,Obruca S,Krzyzanek V,Sedlacek P,Hrubanova K,Samek O,Kucera D,Benesova P,Nebesarova J

更新日期：2016-05-01 00:00:00

abstract：:The purpose of this study was to develop a novel strategy for the isolation and identification of Burkholderia cepacia complex bacteria from the home environment of cystic fibrosis (CF) patients. Water and soil samples were enriched in a broth containing 0.1% l-arabinose, 0.1% l-threonine, and a mixture of selective a...

journal_title：FEMS microbiology letters

authors： Vanlaere E,Coenye T,Samyn E,Van den Plas C,Govan J,De Baets F,De Boeck K,Knoop C,Vandamme P

更新日期：2005-08-15 00:00:00

abstract：:The detection of ancient microbial DNA offers a new approach for the study of infectious diseases, their occurrence, frequency and host-pathogen interaction in historic times and populations. Moreover, data obtained from skeletal and mummified tissue may represent an important completion of contemporary phylogenetic a...

journal_title：FEMS microbiology letters

authors： Zink AR,Reischl U,Wolf H,Nerlich AG

更新日期：2002-08-06 00:00:00

abstract：:Salmonella uses type III secretion systems (TTSS) to deliver pathogenic proteins into the host cells. These translocated effectors induce bacterial internalization and intracellular proliferation by targeting important cellular processes that are conserved among eukaryotes. Here, we assessed the feasibility of perform...

journal_title：FEMS microbiology letters

authors： Alemán A,Fernández-Piñar P,Pérez-Núñez D,Rotger R,Martín H,Molina M

更新日期：2009-06-01 00:00:00

abstract：:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...

journal_title：FEMS microbiology letters

authors： Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

更新日期：1997-01-15 00:00:00

abstract：:The genes for fructosyltransferase (ftf) and glucan-binding protein (gbp) of Streptococcus mutans strain Ingbritt have been cloned in E. coli using bacteriophage and plasmid vectors. A single ftf gene appears to be responsible for the appearance of several FTF of different electrophoretic mobilities. Despite the fact ...

journal_title：FEMS microbiology letters

authors： Aduse-Opoku J,Gilpin ML,Russell RR

更新日期：1989-06-01 00:00:00

abstract：:Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L(-1)) inhibits this lipase activity on a Mueller-Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophen...

journal_title：FEMS microbiology letters

authors： Kuroda M,Nagasaki S,Ito R,Ohta T

更新日期：2007-08-01 00:00:00

abstract：:A newly isolated strain of Variovorax paradoxus could grow on homovanillate and several monohydroxylated phenylacetic acids. During growth on homovanillate, the organism formed separate NAD(P)H-dependent hydroxylases with activity towards 4-hydroxyphenylacetic acid and homovanillate. Homovanillate hydroxylase catalyse...

journal_title：FEMS microbiology letters

authors： Allison N,Turner JE,Wait R

更新日期：1995-12-15 00:00:00

abstract：:The genes encoding the desulforubidin type of dissimilatory sulfite reductase (Dsr) from the sulfate-reducing bacteria Desulfobacter vibrioformis and Desulfobulbus rhabdoformis were cloned and sequenced. Similar to the genes for dissimilatory sulfite reductase from the genera Archaeoglobus, Desulfovibrio and Desulfoto...

journal_title：FEMS microbiology letters

authors： Larsen,Lien T,Birkeland NK

更新日期：2000-05-01 00:00:00

abstract：:An accurate monitoring of disease progression is important to evaluate disease susceptibility phenotypes. Over the years, Arabidopsis thaliana has become the model species to serve as a host in plant-pathogen interactions. Despite the efforts to study genetic mechanisms of host defense, little efforts are made for a t...

journal_title：FEMS microbiology letters

authors： Brouwer M,Lievens B,Van Hemelrijck W,Van den Ackerveken G,Cammue BP,Thomma BP

更新日期：2003-11-21 00:00:00

abstract：:Expression of AT-rich genes from microorganisms such as archaea is often inefficient in Escherichia coli. The trpA and trpB genes encoding the tryptophan synthase subunits were cloned from the hyperthermophilic archaeon Pyrococcus furiosus. No apparent difference in codon bias was found between the genes. However, usi...

journal_title：FEMS microbiology letters

authors： Ishida M,Oshima T,Yutani K

更新日期：2002-11-05 00:00:00

abstract：:The porin-associated protein of Rhodospirillum rubrum FR1 was found to contain a peptidoglycan binding motif. A partial fragment of 179 amino acids, obtained by cleavage of PAP with trypsin, Asp-N protease, and CNBr, was sequenced. Substantial sequence homology was found of the C-terminal part (residues 126-179) of po...

journal_title：FEMS microbiology letters

authors： Neumann U,Schiltz E,Stahl B,Hillenkamp F,Weckesser J

更新日期：1996-04-15 00:00:00

abstract：:An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD g...

journal_title：FEMS microbiology letters

authors： de Queiroz MV,Daboussi MJ

更新日期：2003-01-28 00:00:00

abstract：:The mol% G + C of DNA extracted from seven different isolates of Renibacterium salmoninarum was determined. Organisms studied were from selected geographical areas (U.S.A., Canada, England and France) and were isolated from five different species of salmonid fish. The mol% G + C was determined to be 55.5, higher than ...

journal_title：FEMS microbiology letters

authors： Banner CR,Rohovec JS,Fryer JL

更新日期：1991-03-15 00:00:00

abstract：:Microcystins produced by cyanobacteria are potent and specific hepatotoxins; however, the mechanisms of microcystin-induced hepatotoxicity have not been fully elucidated. The induction of free radical formation and mitochondrial alterations are two major events found in microcystin-treated cultured rat hepatocytes. Th...

journal_title：FEMS microbiology letters

authors： Ding WX,Nam Ong C

更新日期：2003-03-14 00:00:00

abstract：:A broad distribution of the lactococcal IS elements ISS1 [1] and IS904 [2] in several lactococcal plasmids and chromosomal DNA was observed. Hybridization of the ISS1 and IS904 oligonucleotide gene probes with DNA of lactococcal phages showed that none of these tested bacteriophages contained one of the IS elements. O...

journal_title：FEMS microbiology letters

authors： Schäfer A,Jahns A,Geis A,Teuber M

更新日期：1991-05-15 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) is a key enzyme in cellular one-carbon pathway and has been studied in many living organisms from bacteria to higher plants and mammals. However, biochemical and molecular characterization of SHMT from photoautotrophic microorganisms remains a challenge. Here, we isolated the SHM...

journal_title：FEMS microbiology letters

authors： Waditee-Sirisattha R,Sittipol D,Tanaka Y,Takabe T

更新日期：2012-08-01 00:00:00

abstract：:The photoinduced bactericidal capacity of TiO(2) based films was evaluated, using as model organism Pseudomonas aeruginosa. Thin films were obtained by spray pyrolysis; they included undoped, Cu doped, and Al doped TiO(2). Scanning electron microscopy was used to observe the final effect of the irradiated films upon t...

journal_title：FEMS microbiology letters

authors： Amézaga-Madrid P,Nevárez-Moorillón GV,Orrantia-Borunda E,Miki-Yoshida M

更新日期：2002-06-04 00:00:00

abstract：:We report for the first time the production of acyl homoserine lactones (AHLs) by Erwina amylovora, an important quarantine bacterial pathogen that causes fire blight in plants. E. amylovora produces one N-acyl homoserine lactone [a N-(3-oxo-hexanoyl)-homoserine lactone or a N-(3-hydroxy-hexanoyl)-homoserine lactone] ...

journal_title：FEMS microbiology letters

authors： Venturi V,Venuti C,Devescovi G,Lucchese C,Friscina A,Degrassi G,Aguilar C,Mazzucchi U

更新日期：2004-12-15 00:00:00

abstract：:The study was made of the antibacterial effects of three terpene alcohols on Staphylococcus aureus, focusing on the leakage of K+ ions and toxicity over time. The leakage of K+ ions was monitored continuously with a K+-electrode. Our results suggested that the terpene alcohols, namely, farnesol, nerolidol and plaunoto...

journal_title：FEMS microbiology letters

authors： Inoue Y,Shiraishi A,Hada T,Hirose K,Hamashima H,Shimada J

更新日期：2004-08-15 00:00:00

abstract：:Neisseria meningitidis is able to chelate iron from human transferrin (HTF), the main sequestrator of extracellular iron in vivo. Previous workers have reported that a ca. 70 kilodalton (kDa) iron regulated outer membrane protein (FeRP-70) is a highly specific receptor for HTF. We have examined the interaction between...

journal_title：FEMS microbiology letters

authors： Ala'Aldeen DA,Davies HA,Wall RA,Borriello SP

更新日期：1990-05-01 00:00:00

abstract：:Probing protein extracts from exponentially growing and stationary phase cultures of Mycobacterium bovis BCG with anti-phospho amino acid antibodies revealed a 31-kDa anti-phospho threonine antibody-reactive protein specific to growing culture. The corresponding protein was purified via two-dimensional gel electrophor...

journal_title：FEMS microbiology letters

authors： Sinha I,Boon C,Dick T

更新日期：2003-10-10 00:00:00

abstract：:The polysaccharides formed on hot alkaline extraction of the ascomycetous lichen Teloschistes flavicans were fractionated to give two glucans, which were characterised by methylation analysis and 1D and 2D NMR spectroscopy. One was a branched beta-glucan containing (1-->3) and (1-->6) linkages, a structure which is mo...

journal_title：FEMS microbiology letters

authors： Reis RA,Tischer CA,Gorin PA,Iacomini M

更新日期：2002-04-23 00:00:00

abstract：:An obligately anaerobic spirochete designated strain SEBR 4228T (T = type strain) was isolated from an oil field of Congo, Central Africa. The strain grew optimally with a sodium chloride concentration of 5% (sodium chloride concentration) growth range 1.0-10%) at 37 degrees C (growth temperature range 20-40 degrees C...

journal_title：FEMS microbiology letters

authors： Magot M,Fardeau ML,Arnauld O,Lanau C,Ollivier B,Thomas P,Patel BK

更新日期：1997-10-15 00:00:00

abstract：:The prodrug activation system formed by the E. coli codA gene encoding cytosine deaminase (CD) and 5-fluorocytosine (5-FC) developed for selective cancer chemotherapy suffers from a sensitivity limitation in many tumour cells. In an attempt to improve the CD/5-FC suicide association, we combined the E. coli upp gene e...

journal_title：FEMS microbiology letters

authors： Tiraby M,Cazaux C,Baron M,Drocourt D,Reynes JP,Tiraby G

更新日期：1998-10-01 00:00:00

abstract：:DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by us...

journal_title：FEMS microbiology letters

authors： Gowher H,Ehrlich KC,Jeltsch A

更新日期：2001-11-27 00:00:00

abstract：:In the present study, strains of 17 reference Acinetobacter genospecies were investigated by using the polymerase chain reaction (PCR). We used primers to amplify spacer regions between the 16S and 23S genes in the prokaryotic rRNA genetic loci. When the spacer amplification products were resolved by electrophoresis, ...

journal_title：FEMS microbiology letters

authors： Nowak A,Burkiewicz A,Kur J

更新日期：1995-02-15 00:00:00

abstract：:Chondroitin 6-sulfate depolymerizing activity was examined in the culture supernatant of Streptococcus intermedius ATCC 27335. 2-Acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose was split from the substrate. The enzyme(s) was not active upon chondroitin 4-sulfate or dermatan sulfate...

journal_title：FEMS microbiology letters

authors： Hibi E,Osano E,Fujii Y,Moriyama T

更新日期：1989-01-15 00:00:00