Abstract:
:Expression of AT-rich genes from microorganisms such as archaea is often inefficient in Escherichia coli. The trpA and trpB genes encoding the tryptophan synthase subunits were cloned from the hyperthermophilic archaeon Pyrococcus furiosus. No apparent difference in codon bias was found between the genes. However, using a conventional cloning vector having the lac promoter, the trpB gene was expressed poorly in E. coli, whereas the trpA gene was overexpressed. The expression of the trpB gene was remarkably enhanced (>12-fold) by the introduction of an overlapping leader open reading frame. The expression of the trpA gene was also improved ( approximately 1.5-fold). This approach may be useful for overexpressing various kinds of AT-rich genes.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Ishida M,Oshima T,Yutani Kdoi
10.1111/j.1574-6968.2002.tb11433.xkeywords:
subject
Has Abstractpub_date
2002-11-05 00:00:00pages
179-83issue
2eissn
0378-1097issn
1574-6968pii
S0378109702009977journal_volume
216pub_type
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journal_title:FEMS microbiology letters
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abstract::The benzimidazole fungicide thiophanate-methyl is commonly applied to control leaf mould of tomato caused by Cladosporium fulvum in China. In this study, 32 isolates of C. fulvum were examined for their sensitivities to thiophanate-methyl, and two benzimidazole-resistant (BenR) phenotypes BenR1 and BenR2 were identifi...
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