Metagenome microarray for screening of fosmid clones containing specific genes.

abstract：:A class 1 integron was detected in a single multidrug-resistant strain of enterotoxigenice Escherichia coli (ETEC) O159 after examination of 23 clinical E. coli isolates. This isolate was resistant to streptomycin, kanamycin, gentamicin, chloramphenicol and ampicillin. Sequencing of the class 1 integron identified thr...

journal_title：FEMS microbiology letters

authors： Ahmed AM,Shimamoto T

更新日期：2004-06-15 00:00:00

abstract：:During protein synthesis, elongation factor G (EFG) participates at the steps of translocation and ribosome recycling. Fusidic acid (FA) is a bacteriostatic antibiotic, which traps EFG on ribosomes, stalling them on mRNAs. How the bacterial susceptibility to FA is determined, and which of the two functions of EFG (tra...

journal_title：FEMS microbiology letters

authors： Ayyub SA,Lahry K,Dobriyal D,Mondal S,Varshney U

更新日期：2018-07-01 00:00:00

abstract：:Spores of Bacillus species have novel properties, which allow them to lie dormant for years and then germinate under favourable conditions. In the current work, the role of a key metabolic integrator, coenzyme A (CoA), in redox regulation of growing cells and during spore formation in Bacillus megaterium and Bacillus ...

journal_title：FEMS microbiology letters

authors： Zhyvoloup A,Yu BYK,Baković J,Davis-Lunn M,Tossounian MA,Thomas N,Tsuchiya Y,Peak-Chew SY,Wigneshweraraj S,Filonenko V,Skehel M,Setlow P,Gout I

更新日期：2020-12-22 00:00:00

abstract：:Dextranase specifically hydrolyzes dextran and is used to produce functional isomalto-saccharide prebiotics. Moreover, dextranase is used as an additive in mouthwash to remove dental plaque. We cloned and expressed the dextranase gene of the marine bacterium Bacillus aquimaris S5. The length of the BaDex gene was 1788...

journal_title：FEMS microbiology letters

authors： Dong D,Wang X,Deng T,Ning Z,Tian X,Zu H,Ding Y,Wang C,Wang S,Lyu M

更新日期：2021-01-20 00:00:00

abstract：:The gene (open reading frame (ORF) Tm1469, glk) encoding ATP-dependent ROK (repressors, ORFs, sugar kinases) glucokinase (ATP-GLK, EC 2.7.1.2) of the hyperthermophilic bacterium Thermotoga maritima was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparen...

journal_title：FEMS microbiology letters

authors： Hansen T,Schönheit P

更新日期：2003-09-26 00:00:00

abstract：:To test the hypothesis that actA allelic variation contributes to virulence differences among Listeria monocytogenes strains, cell-to-cell spread and intracellular ActA phosphorylation patterns were characterized for 14 wild-type isolates and selected isogenic mutants. Our data show that (i) while actA allelic variati...

journal_title：FEMS microbiology letters

authors： Roberts AJ,Wiedmann M

更新日期：2006-01-01 00:00:00

abstract：:The phyCg gene encoding a new phytase from Citrobacter gillenii was optimized, synthesized, cloned and expressed in Pichia pastoris. Analysis of the amino acid sequence of the enzyme showed that it belongs to the histidine acid phosphatase family. The amino acid sequence of the PhyCg phytase has the highest homology (...

journal_title：FEMS microbiology letters

authors： Tkachenko AA,Kalinina AN,Borshchevskaya LN,Sineoky SP,Gordeeva TL

更新日期：2020-12-21 00:00:00

abstract：:An investigation of mortality of piglets through clinical signs, post-mortem, histopathology and bacteriological analyses revealed the causal organism to be Escherichia coli, mainly O149:K91:K88 which belongs to the enterotoxigenic biotypes. Molecular characterization and epidemiologic analysis elucidated it as shiga-...

journal_title：FEMS microbiology letters

authors： Kanengoni AT,Thomas R,Gelaw AK,Madoroba E

更新日期：2017-02-01 00:00:00

abstract：:During the establishment of Escherichia coli O157:H7 infection, its capacity to adhere to host intestinal epithelial cells is the critical first step in pathogenesis. It also has the capability to form biofilms, and because both are surface activities, we sought to gain insight into a potential linkage between biofilm...

journal_title：FEMS microbiology letters

authors： Puttamreddy S,Minion FC

更新日期：2011-02-01 00:00:00

abstract：:The distribution of the alcohol dehydrogenase gene (adh) among different Archaea was investigated by Southern blot analysis revealing the potentiality of the adh gene as a specific marker for the genus Sulfolobus. Moreover, the in vivo expression of the adh gene from a new isolate of Sulfolobus solfataricus, G theta, ...

journal_title：FEMS microbiology letters

authors： Cannio R,Fiorentino G,Rossi M,Bartolucci S

更新日期：1999-01-01 00:00:00

abstract：:The molecular genetics of indole-3-acetic (IAA) synthesis and regulation in Azospirillum brasilense was investigated in this study. Tn5 mutagenesis was performed and five mutants with decreased IAA production were isolated. Five Tn5-inserted genes from these mutants were cloned and sequenced. Four genes were reported ...

journal_title：FEMS microbiology letters

authors： Xie B,Xu K,Zhao HX,Chen SF

更新日期：2005-07-01 00:00:00

abstract：:Insertion sequences (ISs) are mobile genetic elements widely distributed among bacteria. Their impact on the bacterial genome is multifold, including transfer of genetic information, shuttle of adaptive traits and influence on the genomic content. As a result, ISs play an important role in the organization, plasticity...

journal_title：FEMS microbiology letters

authors： Lysnyansky I,Calcutt MJ,Ben-Barak I,Ron Y,Levisohn S,Methé BA,Yogev D

更新日期：2009-05-01 00:00:00

abstract：:The ecology of aerobic microorganisms is never explored in marine oxygen minimum zone (OMZ) sediments. Here we reveal aerobic bacterial communities along ∼3 m sediment-horizons of the eastern Arabian Sea OMZ. Sulfide-containing sediment-cores retrieved from 530 mbsl (meters beneath the sea-level) and 580 mbsl were exp...

journal_title：FEMS microbiology letters

authors： Bhattacharya S,Roy C,Mandal S,Sarkar J,Rameez MJ,Mondal N,Mapder T,Chatterjee S,Pyne P,Alam M,Haldar PK,Roy R,Fernandes S,Peketi A,Chakraborty R,Mazumdar A,Ghosh W

更新日期：2020-10-16 00:00:00

abstract：:The RcsCDB (Rcs) phosphorelay system regulates capsule synthesis, flagella production and other cellular activities in several enteric bacteria. This system consists of three proteins: the sensor RcsC, the cognate response regulator RcsB and the histidine-containing phosphotransfer protein RcsD (YojN), which is hypoth...

journal_title：FEMS microbiology letters

authors： Pescaretti Mde L,Morero R,Delgado MA

更新日期：2009-11-01 00:00:00

abstract：:A novel bacterial strain, PM2, capable of growing on methanol, was isolated in alkaline conditions from a soil inoculum. This bacterium was characterized at the physiological, biochemical and molecular level. Based on biochemical and molecular data strain PM2 was classified as a novel member of the group of fluorescen...

journal_title：FEMS microbiology letters

authors： Pacheco CC,Passos JF,Moradas-Ferreira P,De Marco P

更新日期：2003-10-24 00:00:00

abstract：:We have performed amplified-fragment length polymorphism (AFLP) fingerprinting on a collection of Salmonella enterica subsp. enterica serovar typhimurium strains with a restriction endonuclease combination (BglII and MfeI) that has previously been used successfully for typing Campylobacter jejuni isolates with high re...

journal_title：FEMS microbiology letters

authors： Lindstedt BA,Heir E,Vardund T,Kapperud G

更新日期：2000-08-01 00:00:00

abstract：:Two thermostable phytases were identified from Thai isolates of Aspergillus japonicus BCC18313 (TR86) and Aspergillus niger BCC18081 (TR170). Both genes of 1404 bp length, coding for putative phytases of 468 amino acid residues, were cloned and transferred into Pichia pastoris. The recombinant phytases, r-PhyA86 and r...

journal_title：FEMS microbiology letters

authors： Promdonkoy P,Tang K,Sornlake W,Harnpicharnchai P,Kobayashi RS,Ruanglek V,Upathanpreecha T,Vesaratchavest M,Eurwilaichitr L,Tanapongpipat S

更新日期：2009-01-01 00:00:00

abstract：:Salmonella enteritidis isolated from poultry infections generated a convoluted colonial morphology after 48 h growth on colonisation factor antigen (CFA) agar at 25 degrees C. A mutant S. enteritidis defective for the elaboration of the SEF17 fimbrial antigen, in which the agf gene cluster was inactivated by insertion...

journal_title：FEMS microbiology letters

authors： Allen-Vercoe E,Dibb-Fuller M,Thorns CJ,Woodward MJ

更新日期：1997-08-01 00:00:00

abstract：:Pseudomonas chlororaphis PA23 is a biocontrol agent that, in addition to producing antifungal compounds, synthesizes polyhydroxyalkanoate (PHA) polymers as a carbon and energy sink. Quorum sensing (QS) and the anaerobic regulator (ANR) are required for PA23-mediated fungal suppression; however, the role of these regul...

journal_title：FEMS microbiology letters

authors： Mohanan N,Gislason A,Sharma PK,Ghergab A,Plouffe J,Levin DB,de Kievit T

更新日期：2019-09-01 00:00:00

abstract：:A different mutation (SMR1B) to SMR1-410 (ilv2), which determines resistance to sulfometuron methyl in Saccharomyces cerevisiae, was cloned by PCR. Sequence analysis indicated a C to T change at position 575 of the ILV2 coding sequence, which results in a proline transition to leucine at position 192. Similarly to SMR...

journal_title：FEMS microbiology letters

authors： Xie Q,Jiménez A

更新日期：1996-04-01 00:00:00

abstract：:A DNA segment located immediately upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7 strain HA1 was cloned and sequenced. This segment contained an open reading frame encoding a predicted protein of 156 amino acids. A database search identified similar open reading frames upstream of the eaeA gene...

journal_title：FEMS microbiology letters

authors： Zhao S,Mitchell SE,Meng J,Doyle MP,Kresovich S

更新日期：1995-11-01 00:00:00

abstract：:Dimethylphthalate (DMP), one of the phthalate esters, is used in the manufacture of plasticizers, insect repellents, and synthetic fibers, and contributes to environmental pollution. In the present study, we report a novel bacterium belonging to the Bacillus sp., which has the ability to utilize DMP as the sole source...

journal_title：FEMS microbiology letters

authors： Niazi JH,Prasad DT,Karegoudar TB

更新日期：2001-03-15 00:00:00

abstract：:Pseudomonas aeruginosa strains exhibiting a heterogeneous mode of growth against carbapenems have been described recently. This study investigated the underlying molecular mechanisms in four genetically unrelated P. aeruginosa clinical isolates that were previously characterized by population analyses as heterogeneous...

journal_title：FEMS microbiology letters

authors： Ikonomidis A,Tsakris A,Kantzanou M,Spanakis N,Maniatis AN,Pournaras S

更新日期：2008-02-01 00:00:00

abstract：:We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi strain Ty2. The gene showed a high degree of similarity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rfaH mutant was constructed by site-directed mutagenesis. This mutant produced a rough lipopolysa...

journal_title：FEMS microbiology letters

authors： Rojas G,Saldías S,Bittner M,Zaldívar M,Contreras I

更新日期：2001-10-16 00:00:00

abstract：:Enterococcal surface protein (Esp) is a cell wall-associated protein of Enterococcus faecalis that has been identified as a potential virulence factor. We used a mouse model to examine whether Esp facilitates intestinal colonization or translocation of E. faecalis to mesenteric lymph nodes. After clindamycin treatment...

journal_title：FEMS microbiology letters

authors： Pultz NJ,Shankar N,Baghdayan AS,Donskey CJ

更新日期：2005-01-15 00:00:00

abstract：:One of the commercial strains of Flammulina velutipes was analyzed for the presence of double-stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration. As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown-colored fruit body. The...

journal_title：FEMS microbiology letters

authors： Magae Y,Hayashi N

更新日期：1999-11-15 00:00:00

abstract：:The fungal inhibitory effects of strain Lactobacillus plantarum MiLAB 393, producing broad-spectrum antifungal compounds, were evaluated. A co-cultivation method was set up to monitor effects on fungal growth and protein expression of growing Aspergillus nidulans with L. plantarum MiLAB 393. The effects of inhibitory ...

journal_title：FEMS microbiology letters

authors： Ström K,Schnürer J,Melin P

更新日期：2005-05-01 00:00:00

abstract：:A succinate semialdehyde dehydrogenase gene (gabD) was identified to be disrupted in a transposon-induced mutant of Ralstonia eutropha exhibiting the phenotype 4-hydroxybutyric acid-leaky. The native gabD gene was cloned by colony hybridization using a homologous gabD-specific DNA probe. DNA sequencing revealed an 145...

journal_title：FEMS microbiology letters

authors： Lütke-Eversloh T,Steinbüchel A

更新日期：1999-12-01 00:00:00

abstract：:Mycoplasma salivarium cells bound the Fc fragment of human immunoglobulin G. The activity was remarkably enhanced by Mn2+, but not by Mg2+ and Ca2+; significantly inhibited by D-mannose; and reduced by pronase treatment of the cells. About 90% of the cells treated with pronase were not disrupted. Sodium dodecyl sulfat...

journal_title：FEMS microbiology letters

authors： Shibata K,Sawa Y,Inoue S,Noda M,Watanabe T

更新日期：1994-11-01 00:00:00

abstract：:The bovine enteric calici-like virus, Newbury agent 1 (NA1) was characterised to determine if it is a member of the Caliciviridae and to establish its antigenic relationship to the established bovine enteric calicivirus Newbury agent 2 (NA2). Solid phase immune electron microscopy (SPIEM) allowed quantification of NA1...

journal_title：FEMS microbiology letters

authors： Dastjerdi AM,Snodgrass DR,Bridger JC

更新日期：2000-11-01 00:00:00