Abstract:
:During protein synthesis, elongation factor G (EFG) participates at the steps of translocation and ribosome recycling. Fusidic acid (FA) is a bacteriostatic antibiotic, which traps EFG on ribosomes, stalling them on mRNAs. How the bacterial susceptibility to FA is determined, and which of the two functions of EFG (translocation or ribosome recycling) is more vulnerable, has remained debatable. The in vivo studies addressing these aspects of FA mediated inhibition of protein synthesis are lacking. Here, we used a system of Escherichia coli strains and their complementation/supplementation with the plasmid borne copies of the inducible versions of EFG and ribosome recycling factor (RRF) genes. Additionally, we investigated FA sensitivity in a strain with increased proportion of stalled ribosomes. We show that the cells with high EFG/RRF (or low RRF/EFG) ratios are more susceptible to FA than those with low EFG/RRF (or high RRF/EFG) ratios. Our in vivo observations are consistent with the recent in vitro reports of dependence of FA susceptibility on EFG/RRF ratios, and the notion that an overriding target of FA is the translocation function of EFG. An applied outcome of our in vivo study is that FA mediated growth inhibition could be facilitated by depletion or inactivation of cellular RRF.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Ayyub SA,Lahry K,Dobriyal D,Mondal S,Varshney Udoi
10.1093/femsle/fny133subject
Has Abstractpub_date
2018-07-01 00:00:00issue
13eissn
0378-1097issn
1574-6968pii
5004850journal_volume
365pub_type
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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更新日期:1998-11-01 00:00:00
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
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更新日期:1994-08-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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pub_type: 信件
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更新日期:2013-09-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2001.tb10519.x
更新日期:2001-02-20 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/j.1574-6968.2008.01135.x
更新日期:2008-05-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/1574-6968.12224
更新日期:2013-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(91)90335-8
更新日期:1991-08-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
doi:10.1093/femsle/fnv156
更新日期:2015-10-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1998.tb13339.x
更新日期:1998-12-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1994.tb06817.x
更新日期:1994-05-01 00:00:00
abstract::Airborne bacteria isolated from a tannery air environment were screened for the property of solubilization of insoluble zinc oxide and zinc phosphate. Out of 10 strains tested, a strain of Pseudomonas aeruginosa (CMG 823) showed the best solubilization and solubilized both zinc oxide and zinc phosphate. Colonies of th...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2002.tb11277.x
更新日期:2002-07-16 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1994.tb06631.x
更新日期:1994-01-15 00:00:00