Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli.

abstract：:PM3 is an Aeromonas-specific bacteriophage which was isolated and characterized on A. hydrophila strain TF7. Spontaneous mutants resistant to PM3 were non-motile having lost their characteristic monopolar flagellum. In addition, purified flagella inactivated PM3. PM3 is the first filamentous bacteriophage isolated on ...

journal_title：FEMS microbiology letters

authors： Merino S,Camprubi S,Tomás JM

更新日期：1990-06-01 00:00:00

abstract：:Symbioses between phototrophs and heterotrophs (a.k.a 'photosymbioses') are extremely common, and range from loose and temporary associations to obligate and highly specialized forms. In the history of life, the most transformative was the 'primary endosymbiosis,' wherein a cyanobacterium was engulfed by a eukaryote a...

journal_title：FEMS microbiology letters

authors： Gavelis GS,Gile GH

更新日期：2018-10-01 00:00:00

abstract：:Staphylococcus carnosus 833, inoculated into sausage meat, increased the level of methyl ketones, which contributed to the cured aroma. These ketones can arise from incomplete beta-oxidation followed by two enzymatic activities: a thioesterase and a decarboxylase. In this study we identified the beta-oxidative pathway...

journal_title：FEMS microbiology letters

authors： Engelvin G,Feron G,Perrin C,Mollé D,Talon R

更新日期：2000-09-01 00:00:00

abstract：:The study compared images of mature Streptococcus mutans biofilms captured at increasing magnification to determine which microscopy method is most acceptable for imaging the biofilm topography and the extracellular polymeric substance (EPS). In vitro S. mutans biofilms were imaged using (1) scanning electron microsco...

journal_title：FEMS microbiology letters

authors： Weber K,Delben J,Bromage TG,Duarte S

更新日期：2014-01-01 00:00:00

abstract：:Changes in gene expression play a critical role in enhancing the ability of cyanobacteria to survive under cold conditions. In the present study, Spirulina platensis cultures were grown at the optimal growth temperature, in the light, before being transferred to dark conditions at 22 degrees C. Two dimensional-differe...

journal_title：FEMS microbiology letters

authors： Jeamton W,Mungpakdee S,Sirijuntarut M,Prommeenate P,Cheevadhanarak S,Tanticharoen M,Hongsthong A

更新日期：2008-04-01 00:00:00

abstract：:The fungal inhibitory effects of strain Lactobacillus plantarum MiLAB 393, producing broad-spectrum antifungal compounds, were evaluated. A co-cultivation method was set up to monitor effects on fungal growth and protein expression of growing Aspergillus nidulans with L. plantarum MiLAB 393. The effects of inhibitory ...

journal_title：FEMS microbiology letters

authors： Ström K,Schnürer J,Melin P

更新日期：2005-05-01 00:00:00

abstract：:Mapping of the plasmid-encoded RNA of the intracellular parasite, Chlamydia trachomatis revealed that the upstream control elements are different from those of other Gram-negative bacteria. A tetranucleotide, AYAA was found near the -10 position, in 5 out of 8 upstream sequences described so far. The plasmid also has ...

journal_title：FEMS microbiology letters

authors： Sriprakash KS,Pearce BJ

更新日期：1990-09-15 00:00:00

abstract：:D-(-)-3-Hydroxybutyrate (3HB) oligomer hydrolase was purified from Paracoccus denitrificans. The enzyme was a monomeric protein with an approximate molecular mass of 31 kDa. The isoelectric point of the enzyme was 5.2. Optimum temperature and pH were 35-40 degrees C and 8.0, respectively. The enzyme activity was not a...

journal_title：FEMS microbiology letters

authors： Ueda S,Sano K,Gao D,Tomihari N,Yamane T,Endo I

更新日期：2002-01-10 00:00:00

abstract：:Riemerella anatipestifer is the causative agent of polyserositis of ducks and geese. We have previously reported that a 3.9-kb plasmid, pCFC1, carries protein genes (vapD1 and vapD2) that are similar to virulence-associated genes of other bacteria. In the present study, we report the complete sequence of a second plas...

journal_title：FEMS microbiology letters

authors： Weng S,Lin W,Chang Y,Chang C

更新日期：1999-10-01 00:00:00

abstract：:Listeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters (prfAP1, prfAP2, and PplcA). The prfAP2 promoter was previously proposed to be sigma B (sigma(B))-dependent. This hypothesis was tested by creating prfA promoter-gus transcriptional fusions in both L. monocytogenes ...

journal_title：FEMS microbiology letters

authors： Schwab U,Bowen B,Nadon C,Wiedmann M,Boor KJ

更新日期：2005-04-15 00:00:00

abstract：:In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb...

journal_title：FEMS microbiology letters

authors： Yang Y,Li C

更新日期：2009-01-01 00:00:00

abstract：:A Ca(2+)/calmodulin-dependent protein kinase (CaMK) gene was cloned and characterized from Arthrobotrys dactyloides, a nematode-trapping fungus. The resulting 373-amino-acid protein, FCaMK, has significant homology to mammalian CaMKs. FCaMK contains a serine/threonine kinase domain followed by a calmodulin-binding dom...

journal_title：FEMS microbiology letters

authors： Tsai PJ,Tu J,Chen TH

更新日期：2002-06-18 00:00:00

abstract：:Legionella pneumophila is a pathogenic bacterium found in freshwater environments that is responsible for pneumonia. People become infected through inhalation of contaminated droplets from water devices, such as cooling towers and showers. It is important to find new treatments that decrease the development of Legione...

journal_title：FEMS microbiology letters

authors： Héchard Y,Ferraz S,Bruneteau E,Steinert M,Berjeaud JM

更新日期：2005-11-01 00:00:00

abstract：:Specific non-typhoid Salmonella serovars carry large virulence plasmids that promote sustained extra-intestinal infections. These plasmids all share a highly conserved 8-kb region containing the spv operon, consisting of the regulatory spvR locus and the four structural spvABCD genes. The SpvR protein belongs to the L...

journal_title：FEMS microbiology letters

authors： Guiney DG,Fang FC,Krause M,Libby S

更新日期：1994-11-15 00:00:00

abstract：:The genus Burkholderia comprises over 28 species and species-specific, recA-based polymerase chain reaction (PCR) tests are available for several species, but not for some soil-inhabiting species including B. fungorum. Previous analysis of several novel rhizospheric, environmental isolates belonging to the B. cepacia ...

journal_title：FEMS microbiology letters

authors： Chan CH,Stead DE,Coutts RH

更新日期：2003-07-15 00:00:00

abstract：:The gene encoding the alpha-subunit of methanol dehydrogenase (mxaF) and its flanking region was isolated from a methylotrophic bacterium, Hyphomicrobium methylovorum GM2. The deduced amino acid sequence of MxaF showed 80, 80, 74 and 66% identity with those of Methylobacterium extorquens AM1, M. organophilum XX, Parac...

journal_title：FEMS microbiology letters

authors： Tanaka Y,Yoshida T,Watanabe K,Izumi Y,Mitsunaga T

更新日期：1997-09-15 00:00:00

abstract：:Oidium sp. has been recovered as an endophyte in Terminalia catappa (tropical chestnut) in Costa Rica. The volatile organic compounds (VOCs) of this organism uniquely and primarily consist of esters of propanoic acid, 2-methyl-, butanoic acid, 2-methyl-, and butanoic acid, 3-methyl-. The VOCs of Oidium sp. are slightl...

journal_title：FEMS microbiology letters

authors： Strobel GA,Spang S,Kluck K,Hess WM,Sears J,Livinghouse T

更新日期：2008-06-01 00:00:00

abstract：:Occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in Japan and Korea. The tet(M) gene was detected in 34 Japanese and Korean isolates, which included Vibrio sp., Lactococcu...

journal_title：FEMS microbiology letters

authors： Kim SR,Nonaka L,Suzuki S

更新日期：2004-08-01 00:00:00

abstract：:Legionella pneumophila, an intracellular parasite of macrophages and protozoa, requires iron for extra- and intracellular growth. In a new screen of a mutant library of L. pneumophila for strains defective for growth on agar media lacking supplemental iron, seven mutants were obtained. All of the mutants had a disrupt...

journal_title：FEMS microbiology letters

authors： Naylor J,Cianciotto NP

更新日期：2004-12-15 00:00:00

abstract：:The effect of light on adenyl cyclase (E.C. 4.6.1.1) and 3':5'-cyclic-AMP-phosphodiesterase (E.C. 3.1.4.17) activity of Trichoderma viride was investigated. Adenyl cyclase proved to be a membrane-associated enzyme, requiring Mn2+ and was activated by light. In contrast, 3':5'-cyclic-AMP-phosphodiesterase showed no lig...

journal_title：FEMS microbiology letters

authors： Kolarova N,Haplová J,Gresík M

更新日期：1992-06-15 00:00:00

abstract：:Mycobacterium leprae incorporated exogenously supplied pyrimidines as bases or nucleosides, but not as a nucleotide, into its nucleic acids. Notably, thymine was incorporated approximately 5 times more rapidly than thymidine by both suspensions of, or intracellular M. leprae. Thymine incorporation was significantly in...

journal_title：FEMS microbiology letters

authors： Wheeler PR

更新日期：1989-01-15 00:00:00

abstract：:Control of Helminthosporium solani, the cause of silver scurf in potato tubers, has been impaired by selection of benzimidazole-resistant strains as a result of repeated use of the fungicide thiabendazole. Identification of thiabendazole-resistant strains of H. solani by conventional techniques takes several weeks. Pr...

journal_title：FEMS microbiology letters

authors： McKay GJ,Cooke LR

更新日期：1997-07-15 00:00:00

abstract：:Characterization of genomic variation among different microbial species, or different strains of the same species, is a field of significant interest with a wide range of potential applications. We have investigated the genomic variation in mycorrhizal fungal genomes through genomic suppressive subtractive hybridizati...

journal_title：FEMS microbiology letters

authors： Murat C,Zampieri E,Vallino M,Daghino S,Perotto S,Bonfante P

更新日期：2011-05-01 00:00:00

abstract：:Hydrogenotrophic methanogens live in a synthrophic relationship with the human gut microbiota as the terminal part of the anaerobic food chain. Methanobrevibacter smithii of the Methanobacteriales is the prevailing archaeal species. Recently, methylotrophic archaea of the novel order Methanomassiliicoccales were isola...

journal_title：FEMS microbiology letters

authors： Vanderhaeghen S,Lacroix C,Schwab C

更新日期：2015-07-01 00:00:00

abstract：:DNA microarray technology was used to survey changes in gene expression in Pseudomonas fluorescens after mitomycin C treatment. As expected, genes associated with the SOS response were upregulated, such as those encoding the recombination protein RecA, DNA repair protein RecN, excinuclease ABC subunit A UvrA, and the ...

journal_title：FEMS microbiology letters

authors： Jin H,Retallack DM,Stelman SJ,Hershberger CD,Ramseier T

更新日期：2007-04-01 00:00:00

abstract：:Gene disruption experiments play an important role in the functional characterization of genes in mycobacteria and rely mostly on the use of one or two antibiotic resistance markers. We have developed a system for mycobacteria which features both the advantages of the use of antibiotic resistance markers for gene disr...

journal_title：FEMS microbiology letters

authors： Malaga W,Perez E,Guilhot C

更新日期：2003-02-28 00:00:00

abstract：:The locus of enterocyte effacement pathogenicity island confers the attaching and effacing histopathology on epithelial cells infected with enteropathogenic and enterohemorrhagic Escherichia coli. We investigated the site of insertion of the locus of enterocyte effacement in E. coli strains in relation to their evolut...

journal_title：FEMS microbiology letters

authors： Wieler LH,McDaniel TK,Whittam TS,Kaper JB

更新日期：1997-11-01 00:00:00

abstract：:A lipopolysaccharide mutant of Leptospira interrogans (LaiMut) was obtained by growth in the presence of an agglutinating monoclonal antibody (mAb) against lipopolysaccharide. Agglutination reactions with anti-lipopolysaccharide mAbs and polyclonal antibodies showed that LaiMut had lost some serogroup Icterohaemorrhag...

journal_title：FEMS microbiology letters

authors： Zapata S,Trueba G,Bulach DM,Boucher D,Adler B,Hartskeerl R

更新日期：2010-08-01 00:00:00

abstract：:Haemin (iron protoporphyrin IX) is an essential growth factor for the periodontal pathogen. Porphyromonas gingivalis. Iron protoporphyrin IX (IPP IX) binding to the avirulent P. gingivalis beige variant (W50/BE1) and the black-pigmenting parent wild-type strain W50 was quantified. W50/BE1 grown in a chemostat under ha...

journal_title：FEMS microbiology letters

authors： Smalley JW,Birss AJ,McKee AS,Marsh PD

更新日期：1996-07-15 00:00:00

abstract：:mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These st...

journal_title：FEMS microbiology letters

authors： Saito T,Okano K,Park HD,Itayama T,Inamori Y,Neilan BA,Burns BP,Sugiura N

更新日期：2003-12-12 00:00:00