Cytochrome c maturation proteins are critical for in vivo growth of Legionella pneumophila.

Abstract:

:Legionella pneumophila, an intracellular parasite of macrophages and protozoa, requires iron for extra- and intracellular growth. In a new screen of a mutant library of L. pneumophila for strains defective for growth on agar media lacking supplemental iron, seven mutants were obtained. All of the mutants had a disruption in the cytochrome c maturation (ccm) locus; two had insertions in ccmB, two in ccmC, and three in ccmF. The ccm mutants were unable to multiply within macrophage-like cells (i.e., U937 and THP-1 cells) and Hartmannella vermiformis amoebae. A competition assay in A/J mice revealed that ccm mutants are severely defective for growth within the lung. Taken together, these data confirm that ccm and cytochrome c maturation proteins are required for L. pneumophila growth in low iron, intracellular infection, and virulence.

journal_name

FEMS Microbiol Lett

authors

Naylor J,Cianciotto NP

doi

10.1016/j.femsle.2004.10.028

keywords:

subject

Has Abstract

pub_date

2004-12-15 00:00:00

pages

249-56

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(04)00774-8

journal_volume

241

pub_type

杂志文章
  • Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge.

    abstract::The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by an emergent, high virulent Aeromonas hydrophila (vAh) in channel catfish, Ictalurus punctatus Adipose fin clipped catfish were challenged with vAh using a waterborne challenge method, and the distribution of vAh over a ti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw080

    authors: Zhang D,Moreira GS,Shoemaker C,Newton JC,Xu DH

    更新日期:2016-05-01 00:00:00

  • Alkali production by oral bacteria and protection against dental caries.

    abstract::pH is a key environmental factor affecting the physiology, ecology and pathogenicity of the oral biofilms colonizing the hard tissues of the human mouth. Much attention has been focused on the production of organic acids through the metabolism of carbohydrates by pathogenic oral bacteria. Now, evidence is emerging tha...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2000.tb09393.x

    authors: Burne RA,Marquis RE

    更新日期:2000-12-01 00:00:00

  • Distribution of the antiseptic-resistance genes qacE and qacE delta 1 in gram-negative bacteria.

    abstract::The distribution of the antiseptic-resistance genes qacE and qacE delta 1 was studied in a large number of Gram-negative bacteria by a method that included the polymerase chain reaction (PCR). A total of 117 strains of Gram-negative bacteria, isolated from clinical or environmental sources, was used in this analysis. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12857.x

    authors: Kazama H,Hamashima H,Sasatsu M,Arai T

    更新日期:1998-02-15 00:00:00

  • Isolation and characterization of Escherichia coli O157 from retail beef and bovine feces in Thailand.

    abstract::Antibody to Escherichia coli O157 lipopolysaccharide was detected in the sera of healthy individuals more frequently in Southern Thailand than in Japan. The result suggested possible exposure of Thai people to E. coli O157. E. coli O157:H7 or O157:H(-) was isolated from four of 95 retail beef and one of 55 bovine fece...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08919.x

    authors: Vuddhakul V,Patararungrong N,Pungrasamee P,Jitsurong S,Morigaki T,Asai N,Nishibuchi M

    更新日期:2000-01-15 00:00:00

  • Prevotella intermedia induces matrix metalloproteinase-9 expression in human periodontal ligament cells.

    abstract::Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia, a major periodontal pathogen, on MMP-9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP-9 mRNA expression was measured...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01140.x

    authors: Guan SM,Shu L,Fu SM,Liu B,Xu XL,Wu JZ

    更新日期:2008-06-01 00:00:00

  • Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs.

    abstract::Eight-day-old embryonated hen's eggs were used as a model to study Mycobacterium avium virulence. Strains isolated from human patients caused 20-90% mortality when eggs were infected by injection of bacterial suspensions into the amniotic sac. Virulence of examined strains subsequently decreased with passage through e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09297.x

    authors: Long EG,Ewing EP Jr,Bartlett JH,Horsburgh CR Jr,Birkness KA,Yakrus MA,Newman GW,Quinn FD

    更新日期:2000-09-15 00:00:00

  • Production of acylated homoserine lactone by gram-positive bacteria isolated from marine water.

    abstract::Acylated homoserine lactone (AHL)-based quorum sensing (QS) has been reported to be present only in Gram-negative microorganisms. Isolation of a novel Gram-positive microorganism from sea water, capable of producing AHL, is reported here. The isolate (GenBank: JF915892, designated as MPO) belonging to the Exiguobacter...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12123

    authors: Biswa P,Doble M

    更新日期:2013-06-01 00:00:00

  • Purification and characterization from human parotid secretion of a peptide which inhibits hemagglutination of Bacteroides gingivalis 381.

    abstract::A peptide from human parotid secretion which inhibited hemagglutination of Bacteroides gingivalis 381 was purified by ultrafiltration followed by DEAE-Sephadex A-25 column chromatography and by gel filtration on Sephadex G-25, and then by reversed-phase HPLC. The complete amino acid sequence of the peptide, determined...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90316-i

    authors: Murakami Y,Amano A,Takagaki M,Shizukuishi S,Tsunemitsu A,Aimoto S

    更新日期:1990-11-01 00:00:00

  • Direct inhibition of cytochrome c-induced caspase activation in vitro by Toxoplasma gondii reveals novel mechanisms of interference with host cell apoptosis.

    abstract::The intracellular parasite Toxoplasma gondii is known to inhibit apoptosis of its host cell. The molecular mechanisms of this interference are, however, not yet completely understood. We show here that viable parasites prominently inhibited the activation of caspase 3/7 induced by cytochrome c, dATP and dithiothreitol...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00241.x

    authors: Keller P,Schaumburg F,Fischer SF,Häcker G,Gross U,Lüder CG

    更新日期:2006-05-01 00:00:00

  • Multiplicity and expression of xylanases in the rumen fungus Neocallimastix frontalis.

    abstract::The time course production of xylanolytic enzymes by the rumen anaerobic fungus Neocallimastix frontalis was studied during growth on different carbon sources and revealed using isoelectric focusing and immunoblotting. A constant low level of endoxylanase expression was observed in glucose medium. A high level of xyla...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13066.x

    authors: Gomez de Segura B,Durand R,Fèvre M

    更新日期:1998-07-01 00:00:00

  • Antibacterial action of acriflavine hydrochloride for eradication of the gastric pathogen Helicobacter pylori.

    abstract::Helicobacter pylori, a type 1 carcinogen, accounts for numerous gastric cancer-related deaths worldwide. Repurposing existing drugs or developing new ones for a combinatorial approach against increasing antimicrobial resistance is the need of the hour. This study highlights the efficacy of acriflavine hydrochloride (A...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa178

    authors: Tehlan A,Karmakar BC,Paul S,Kumar R,Kaur I,Ghosh A,Mukhopadhyay AK,Dhar SK

    更新日期:2020-11-23 00:00:00

  • Phylogeny of the genus Azospirillum based on 16S rDNA sequence.

    abstract::The 16S rDNA of 17 strains of Azospirillum, 14 assigned to one of the known species A. amazonense, A. brasilense, A. halopraeferens, A. irakense and A. lipoferum, and the other three of uncertain taxonomic position, was sequenced after polymerase chain reaction amplification and analysed in order to investigate the ph...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07579.x

    authors: Fani R,Bandi C,Bazzicalupo M,Ceccherini MT,Fancelli S,Gallori E,Gerace L,Grifoni A,Miclaus N,Damiani G

    更新日期:1995-06-15 00:00:00

  • Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7.

    abstract::The molecular mechanisms controlling expression of the long polar fimbriae 2 (Lpf2) of enterohemorrhagic Escherichia coli (EHEC) O157:H7 were evaluated. Primer extension was used to locate the lpfA2 transcriptional start site in EHEC strain EDL933 at 171 bp upstream of the lpfA2 start codon. Semi-quantitative RT-PCR d...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12513

    authors: Arenas-Hernández MM,Rojas-López M,Medrano-López A,Nuñez-Reza KJ,Puente JL,Martínez-Laguna Y,Torres AG

    更新日期:2014-08-01 00:00:00

  • The immunosuppressive drug leflunomide affects mating-pheromone response and sporulation by different mechanisms in Saccharomyces cerevisiae.

    abstract::Leflunomide (LFM) is a novel anti-inflammatory and immunosuppressive drug, and inhibits the growth of cytokine-stimulated lymphoid cells in vitro. The effect of LFM on haploid and diploid cells of Saccharomyces cerevisiae was investigated to elucidate the molecular mechanism of action of the drug. Using a halo assay, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09319.x

    authors: Fujimura H

    更新日期:2000-10-01 00:00:00

  • Assessing the diversity of the g23 gene of T4-like bacteriophages from Lake Baikal with high-throughput sequencing.

    abstract::Based on second generation sequencing (MiSeq platform, Illumina), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obt...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx264

    authors: Potapov S,Belykh O,Krasnopeev A,Gladkikh A,Kabilov M,Tupikin A,Butina T

    更新日期:2018-02-01 00:00:00

  • Expression and purification of Clostridium perfringens beta-toxin glutathione S-transferase fusion protein.

    abstract::The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07731.x

    authors: Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

    更新日期:1995-08-01 00:00:00

  • High-quality mutant libraries of Xanthomonas oryzae pv. oryzae and X. campestris pv. campestris generated by an efficient transposon mutagenesis system.

    abstract::A novel transposon mutagenesis system for the phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X. campestris pv. campestris (Xcc) was developed using a Tn5-based transposome. A highly efficient transformation up to 10(6) transformants per microg transposon DNA was obtained. Southern blot and thermal as...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00583-4

    authors: Sun Q,Wu W,Qian W,Hu J,Fang R,He C

    更新日期:2003-09-12 00:00:00

  • From bioremediation to biowarfare: on the impact and mechanism of type IV secretion systems.

    abstract::Type IV secretion systems are employed by a wide variety of Gram-negative microorganisms for the translocation of macromolecules across the cell envelope. The translocated substrates (proteins, protein-DNA complexes and DNA) are as diverse as the organisms on the donor and recipient side of the translocation process. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.femsle.2005.09.030

    authors: Baron C

    更新日期:2005-12-15 00:00:00

  • The heme-copper oxidase family consists of three distinct types of terminal oxidases and is related to nitric oxide reductase.

    abstract::Among aerobic prokaryotes, many different terminal oxidase complexes have been described. Sequence comparison has revealed that the aa3-type cytochrome c oxidase and the bo3-type quinol oxidase are variations on the same theme: the heme-copper oxidase. A third member of this family has recently been recognized: the cb...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.1994.tb07067.x

    authors: van der Oost J,de Boer AP,de Gier JW,Zumft WG,Stouthamer AH,van Spanning RJ

    更新日期:1994-08-01 00:00:00

  • Molecular characterization of beta-lactamase genes blaA and blaB of Yersinia enterocolitica biovar 1A.

    abstract::The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00191.x

    authors: Sharma S,Mittal S,Mallik S,Virdi JS

    更新日期:2006-04-01 00:00:00

  • Photoinduced bactericidal activity against Pseudomonas aeruginosa by TiO(2) based thin films.

    abstract::The photoinduced bactericidal capacity of TiO(2) based films was evaluated, using as model organism Pseudomonas aeruginosa. Thin films were obtained by spray pyrolysis; they included undoped, Cu doped, and Al doped TiO(2). Scanning electron microscopy was used to observe the final effect of the irradiated films upon t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11222.x

    authors: Amézaga-Madrid P,Nevárez-Moorillón GV,Orrantia-Borunda E,Miki-Yoshida M

    更新日期:2002-06-04 00:00:00

  • The Pseudomonas aeruginosa phaG gene product is involved in the synthesis of polyhydroxyalkanoic acid consisting of medium-chain-length constituents from non-related carbon sources.

    abstract::We recently identified the phaG(Pp) gene encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase in Pseudomonas putida, which directly links the fatty acid de novo biosynthesis and polyhydroxyalkanoate (PHA) biosynthesis. An open reading frame (ORF) of which the deduced amino acid sequence shared about 57% identity with P...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09023.x

    authors: Hoffmann N,Steinbüchel A,Rehm BH

    更新日期:2000-03-15 00:00:00

  • Molecular biology of Bacillus subtilis cytochromes.

    abstract::Bacillus subtilis cells must have cytochromes for growth and can synthesize cytochromes of a-, b-, c-, d-, and o-types. After a long lag, our knowledge of the structure, genetics and specific role for these cytochromes is now growing exponentially as the result of recent research. This progress is reviewed here and in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.1992.tb14025.x

    authors: von Wachenfeldt C,Hederstedt L

    更新日期:1992-12-15 00:00:00

  • Hydrocarbon utilization within a diesel-degrading bacterial consortium.

    abstract::Diesel fuel is a common environmental pollutant comprised of a large number of both aromatic and aliphatic hydrocarbons. The microbial degradation of individual hydrocarbons has been well characterized, however, the community dynamics within a system degrading a complex pollutant such as diesel fuel are still poorly u...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01871.x

    authors: Ciric L,Philp JC,Whiteley AS

    更新日期:2010-02-01 00:00:00

  • The activity and stability of cell-associated activity of bovicin HC5, a bacteriocin from Streptococcus bovis HC5.

    abstract::Streptococcus bovis HC5 cultures released a broad spectrum lantibiotic, bovicin HC5, into the cell-fee culture supernatant after they reached stationary phase, but most of the antibacterial activity remained cell-associated. Cell-associated bovicin HC5 was more resistant to degradation by Pronase E than the cell-free ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01155.x

    authors: Xavier BM,Houlihan AJ,Russell JB

    更新日期:2008-06-01 00:00:00

  • Characterization of Bacillus strains from apple and pear trees in South Africa antagonistic to Erwinia amylovora.

    abstract::In order to find reasons for the absence of fire blight in most countries of the Southern hemisphere, bark samples from apple and pear trees in orchards of the Western Cape region in South Africa were extracted for bacteria which could be antagonistic to Erwinia amylovora. Screening was done in the late growth season ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11232.x

    authors: Jock S,Völksch B,Mansvelt L,Geider K

    更新日期:2002-06-04 00:00:00

  • Anaerobic degradation of protocatechuate (3,4-dihydroxybenzoate) by Thauera aromatica strain AR-1.

    abstract::The denitrifying bacterium Thauera aromatica strain AR-1 grows anaerobically with protocatechuate (3,4-dihydroxybenzoate (DHB)) as sole energy and carbon source. This bacterium harbors two distinct pathways for degradation of aromatic compounds, the benzoyl-coenzyme A (CoA) pathway for benzoate degradation and the hyd...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11257.x

    authors: Philipp B,Kemmler D,Hellstern J,Gorny N,Caballero A,Schink B

    更新日期:2002-06-18 00:00:00

  • Metabolomics approach used for understanding temperature-related pectinase activity in Bacillus licheniformis DY2.

    abstract::Pectinases are enzymes that catalyze pectin degradation. There is a global demand for pectinases because of their wide utility and catalytic efficiency. Optimization of the fermentation process to increase the pectolytic enzyme activity is generally practiced to lower process costs, but whether temperature influences ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny255

    authors: Guan Y,Yin D,Du X,Ye X

    更新日期:2018-12-01 00:00:00

  • Conserved motifs in the CRISPR leader sequence control spacer acquisition levels in Type I-D CRISPR-Cas systems.

    abstract::Integrating short DNA fragments at the correct leader-repeat junction is key to successful CRISPR-Cas memory formation. The Cas1-2 proteins are responsible to carry out this process. However, the CRISPR adaptation process additionally requires a DNA element adjacent to the CRISPR array, called leader, to facilitate ef...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz129

    authors: Kieper SN,Almendros C,Brouns SJJ

    更新日期:2019-06-01 00:00:00

  • Has the prehistoric ice-man contributed to the preservation of living fungal spores?

    abstract::There are arguments against the conclusion drawn by Haselwandter and Ebner that fungal spores have survived for some 5300 years on hay padding in the leather boots of a frozen body discovered in the Austrian Alps. According to cryobiological experience, long-term survival of fungal spores is very unlikely at temperatu...

    journal_title:FEMS microbiology letters

    pub_type: 评论,杂志文章

    doi:10.1111/j.1574-6968.1994.tb06998.x

    authors: Gams W,Stalpers JA

    更新日期:1994-07-01 00:00:00