Conserved motifs in the CRISPR leader sequence control spacer acquisition levels in Type I-D CRISPR-Cas systems.

abstract：:Porphyromonas gingivalis produces a trypsin-like enzyme, Protease I, which is thought to be an important virulence determinant of the organism in adult periodontal disease. Protease I is transiently inhibited by physiological inhibitors of human thrombin. The aim of the present work was to establish whether Protease I...

journal_title：FEMS microbiology letters

authors： Curtis MA,Macey M,Slaney JM,Howells GL

更新日期：1993-06-15 00:00:00

abstract：:A multiplex-polymerase chain reaction (PCR) assay based on one-step amplification and detection of two different mycobacterial genomic fragments was designed for differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from a 500-bp genomic fragment which is well c...

journal_title：FEMS microbiology letters

authors： Shah DH,Verma R,Bakshi CS,Singh RK

更新日期：2002-08-27 00:00:00

abstract：:Wood-rotting fungi possess remarkably diverse extracellular oxidation mechanisms, including enzymes, such as laccase and peroxidases, and Fenton chemistry. The ability to biologically drive Fenton chemistry by the redox cycling of quinones has previously been reported to be present in both ecologically diverging main ...

journal_title：FEMS microbiology letters

authors： Krueger MC,Bergmann M,Schlosser D

更新日期：2016-06-01 00:00:00

abstract：:Members of the Streptococcus bovis group are frequent colonizers of the intestinal tract, which can also cause endocarditis. However, their ability to adhere to and colonize host tissues and the factors associated with pathogenicity are largely unknown. Here, we assessed 17 endocarditis-derived human isolates [identif...

journal_title：FEMS microbiology letters

authors： Sillanpää J,Nallapareddy SR,Singh KV,Ferraro MJ,Murray BE

更新日期：2008-12-01 00:00:00

abstract：:A 1.8-kb cryptic plasmid pFR18 was isolated from Leuconostoc mesenteroides ssp. mesenteroides FR52 and characterized. The identification of single-stranded DNA intermediate (ssDNA) in Leuconostoc demonstrated that the replication of pFR18 is directed by a rolling-circle mechanism (RCR). Sequence analysis revealed a si...

journal_title：FEMS microbiology letters

authors： Biet F,Cenatiempo Y,Fremaux C

更新日期：1999-10-15 00:00:00

abstract：:A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain B...

journal_title：FEMS microbiology letters

authors： Xue J,Liang G,Crickmore N,Li H,He K,Song F,Feng X,Huang D,Zhang J

更新日期：2008-03-01 00:00:00

abstract：:The multiple antibiotic resistance operon (marRAB) is a member of the multidrug resistance (mdr) systems. Similar to other mdr systems, this operon when induced encodes resistance to structurally and functionally unrelated antibiotics. marRAB has been shown to be conserved in the family Enterobacteriaceae, but within ...

journal_title：FEMS microbiology letters

authors： Kunonga NI,Sobieski RJ,Crupper SS

更新日期：2000-06-15 00:00:00

abstract：:For the establishment of a diagnostic tool for mycobacterial species, a part of the dnaA gene was amplified and sequenced from clinically relevant 27 mycobacterial species as well as 49 clinical isolates. Sequence variability in the amplified segment of the dnaA gene allowed the differentiation of all species except f...

journal_title：FEMS microbiology letters

authors： Mukai T,Miyamoto Y,Yamazaki T,Makino M

更新日期：2006-01-01 00:00:00

abstract：:The gene encoding the alpha-subunit of methanol dehydrogenase (mxaF) and its flanking region was isolated from a methylotrophic bacterium, Hyphomicrobium methylovorum GM2. The deduced amino acid sequence of MxaF showed 80, 80, 74 and 66% identity with those of Methylobacterium extorquens AM1, M. organophilum XX, Parac...

journal_title：FEMS microbiology letters

authors： Tanaka Y,Yoshida T,Watanabe K,Izumi Y,Mitsunaga T

更新日期：1997-09-15 00:00:00

abstract：:Cronobacter sakazakii is associated with severe and often fatal cases of infant meningitis and necrotizing enterocolitis. The form of meningitis differs from that due to Neisseria meningitidis and Streptococcus spp., in that it is highly invasive and destructive towards human brain cells. However, there is relatively ...

journal_title：FEMS microbiology letters

authors： Alzahrani H,Winter J,Boocock D,De Girolamo L,Forsythe SJ

更新日期：2015-06-01 00:00:00

abstract：:Chronological analysis of 125 Vibrio cholerae O139 strains isolated during 1993-2005 in Kolkata revealed the prevalence of two new genotypes of cholera toxin (CT) and novel combinations of ctxB and rstR alleles resulting in variant CTX prophages. One of the new genotypes of ctxB, which first appeared in 1996 with the ...

journal_title：FEMS microbiology letters

authors： Raychoudhuri A,Mukherjee P,Ramamurthy T,Nandy RK,Takeda Y,Nair GB,Mukhopadhyay AK

更新日期：2010-02-01 00:00:00

abstract：:The gene (open reading frame (ORF) Tm1469, glk) encoding ATP-dependent ROK (repressors, ORFs, sugar kinases) glucokinase (ATP-GLK, EC 2.7.1.2) of the hyperthermophilic bacterium Thermotoga maritima was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparen...

journal_title：FEMS microbiology letters

authors： Hansen T,Schönheit P

更新日期：2003-09-26 00:00:00

abstract：:Chemical and molecular taxonomic studies were performed on a representative strain of some lactic acid bacteria of unknown taxonomic position isolated from salmonid fish. The results demonstrate that the fish bacterium represents a new species of the genus Lactococcus for which the name Lactococcus piscium sp. nov. is...

journal_title：FEMS microbiology letters

authors： Williams AM,Fryer JL,Collins MD

更新日期：1990-03-01 00:00:00

abstract：:The cellulase-producing fungal strain Y-94, isolated in Japan and invalidly described as Acremonium cellulolyticus nom. nud. strain Y-94, seldom forms enteroarthric conidia under nutrient starvation conditions. Phylogenetic analysis using ITS1-5.8S-ITS2 and RNA polymerase II large subunit gene sequences revealed that ...

journal_title：FEMS microbiology letters

authors： Fujii T,Hoshino T,Inoue H,Yano S

更新日期：2014-02-01 00:00:00

abstract：:Transcription of the sigA and sigB genes of Brevibacterium lactofermentum, encoding the principal sigma factors of RNA polymerase, has been investigated by Northern blot and primer extension analysis. Northern hybridizations revealed that sigA is transcribed as a monocistronic mRNA of 1.7 kb and sigB gives two transcr...

journal_title：FEMS microbiology letters

authors： Oguiza JA,Marcos AT,Martín JF

更新日期：1997-08-01 00:00:00

abstract：:Expression of the dimethylsulfoxide respiratory (dor) operon of Rhodobacter is regulated by oxygen, light intensity and availability of substrate. Since dimethylsulfoxide reductase contains a pterin molybdenum cofactor, the role of molybdate in the regulation of dor operon expression was investigated. In this report w...

journal_title：FEMS microbiology letters

authors： Solomon PS,Shaw AL,Young MD,Leimkuhler S,Hanson GR,Klipp W,McEwan AG

更新日期：2000-09-15 00:00:00

abstract：:Acinetobacter baumannii strain A148, a clinical isolate resistant to imipenem (MIC = 32 mg l-1), synthesized two beta-lactamases with pIs 6.3 and > 9.2. The pI 6.3 enzyme hydrolyzed the penicillins, including isoxazoylpenicillins, first-, second- and, to a lesser extent, third-generation cephalosporins. It was inhibit...

journal_title：FEMS microbiology letters

authors： Hornstein M,Sautjeau-Rostoker C,Péduzzi J,Vessières A,Hong LT,Barthélémy M,Scavizzi M,Labia R

更新日期：1997-08-15 00:00:00

abstract：:Molecular microbial ecology studies are heavily reliant on 'Universal' 16S rRNA gene primers for elucidating microbial community structure and composition, and yet primer design and optimization is often overlooked. Primers that exhibit minor biases due to primer-template mismatches can substantially alter the pool of...

journal_title：FEMS microbiology letters

authors： Thomas MC,Thomas DK,Selinger LB,Inglis GD

更新日期：2011-07-01 00:00:00

abstract：:Falcipain-2A, the cysteine protease of Plasmodium falciparum has been proposed as a good drug target. This study evaluated the suitability of Plasmodium berghei as the animal model and reports the first functional expression and characterization of the falcipain-2A orthologue, berghepain-2. Comparative studies reveale...

journal_title：FEMS microbiology letters

authors： Chan C,Goh LL,Sim TS

更新日期：2005-08-15 00:00:00

abstract：:The genome sequence of Roseovarius sp. strain 217 indicated that many pathway enzymes found in other organisms for the degradation of taurine are represented, but that a novel, apparently energy-dependent pathway is involved in the conversion of acetyl phosphate to acetyl CoA. Thus, an ABC transporter for taurine coul...

journal_title：FEMS microbiology letters

authors： Baldock MI,Denger K,Smits TH,Cook AM

更新日期：2007-06-01 00:00:00

abstract：:Because probiotic effects are strain dependent, genomic explanations of these differences will contribute to understanding their mechanisms of action. The genomic sequence of the Bifidobacterium longum probiotic strain NCC2705 was determined, but little is known about the genetic diversity between strains of this spec...

journal_title：FEMS microbiology letters

authors： Delcenserie V,Lessard MH,LaPointe G,Roy D

更新日期：2008-03-01 00:00:00

abstract：:Nocardia asteroides complex organisms derived from human specimens between 1979 and 1992 were identified to the species level. Of 117 N. asteroides complex organisms, 34 (29%) were N. farcinica, 28 (24%) were N. nova, and 55 (47%) were N. asteroides sensu stricto. An analysis of the specimen sites from which the organ...

journal_title：FEMS microbiology letters

authors： Desmond EP,Flores M

更新日期：1993-07-01 00:00:00

abstract：:Cell differentiation is one of the marks of multicellular organisms. Terminally specialised nitrogen-fixing cells, termed heterocysts, evolved in filamentous cyanobacteria more than 2 Gya. The development of their spacing pattern has been thoroughly investigated in model organisms such as Anabaena sp. PCC 7120. This p...

journal_title：FEMS microbiology letters

authors： Antonaru LA,Nürnberg DJ

更新日期：2017-08-15 00:00:00

abstract：:Methanogenic archaea conserve energy for growth by reducing some one- and two-carbon compounds to methane and concomitantly generating an ion motive force. Growth of Methanosarcina acetivorans on carbon monoxide (CO) is peculiar as it involves formation of, besides methane, formate, acetate and methylated thiols. It h...

journal_title：FEMS microbiology letters

authors： Oelgeschläger E,Rother M

更新日期：2009-03-01 00:00:00

abstract：:Synthesis of nitrate reductase in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942 took place at a slow rate when the cells were incubated without a supply of inorganic carbon, but addition to these cells of CO(2)/bicarbonate or, in a Synechococcus strain transformed with a gene encoding a 2-oxoglutara...

journal_title：FEMS microbiology letters

authors： Vázquez-Bermúdez MF,Herrero A,Flores E

更新日期：2003-04-25 00:00:00

abstract：:The development of natural antibodies to Lactococcus lactis in three inbred strains of mice and the effect of inoculating L. lactis into these mice has been investigated. All animals developed detectable levels of natural (systemic and secretory) anti-lactococcal antibodies. Systemic anti-lactococcal antibodies were p...

journal_title：FEMS microbiology letters

authors： Norton PM,Brown HW,Le Page RW

更新日期：1994-07-15 00:00:00

abstract：:Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (...

journal_title：FEMS microbiology letters

authors： Liu X,Feng Z,Harris NB,Cirillo JD,Bercovier H,Barletta RG

更新日期：2001-08-21 00:00:00

abstract：:Infections caused by Streptococcus suis, a major swine pathogen, include meningitis, arthritis, pneumonia and septicaemia. In this study, we investigated interactions that may occur between human brain microvascular endothelial cells (HBMEC), the main constituent of the blood-brain barrier, and S. suis. We show that S...

journal_title：FEMS microbiology letters

authors： Jobin MC,Fortin J,Willson PJ,Gottschalk M,Grenier D

更新日期：2005-11-01 00:00:00

abstract：:A peptide from human parotid secretion which inhibited hemagglutination of Bacteroides gingivalis 381 was purified by ultrafiltration followed by DEAE-Sephadex A-25 column chromatography and by gel filtration on Sephadex G-25, and then by reversed-phase HPLC. The complete amino acid sequence of the peptide, determined...

journal_title：FEMS microbiology letters

authors： Murakami Y,Amano A,Takagaki M,Shizukuishi S,Tsunemitsu A,Aimoto S

更新日期：1990-11-01 00:00:00

abstract：:The genus Burkholderia comprises over 28 species and species-specific, recA-based polymerase chain reaction (PCR) tests are available for several species, but not for some soil-inhabiting species including B. fungorum. Previous analysis of several novel rhizospheric, environmental isolates belonging to the B. cepacia ...

journal_title：FEMS microbiology letters

authors： Chan CH,Stead DE,Coutts RH

更新日期：2003-07-15 00:00:00