Abstract：
:The molecular mechanisms controlling expression of the long polar fimbriae 2 (Lpf2) of enterohemorrhagic Escherichia coli (EHEC) O157:H7 were evaluated. Primer extension was used to locate the lpfA2 transcriptional start site in EHEC strain EDL933 at 171 bp upstream of the lpfA2 start codon. Semi-quantitative RT-PCR demonstrated that the highest lpfA2 expression occurs between an OD600 of 1.0 and 1.2 in DMEM at pH 6.5 and 37 °C. The level of lpfA2 transcription at OD600 1.2 and pH 6.5 was four times greater than that at pH 7.2. Although lpfA2 expression was decreased under iron-depleted conditions, its expression was increased in a ferric-uptake-regulator (Fur) mutant strain. The lpfA2 transcript was 0.7 and 2 times more abundant in wt EHEC grown in DMEM pH 6.5 plus iron and MacConkey broth at 25 °C, respectively, than in DMEM at pH 6.5. The lpf2 expression in DMEM pH 6.5 plus iron and bile salts was 2.7 times more abundant than baseline conditions. Further, transcription in the EDL933∆fur was 0.6 and 0.8 times higher as compared with the wt strain grown in DMEM pH 6.5 plus iron and MacConkey broth, respectively. Electrophoretic mobility shift assays showed that purified Fur interacts with the lpf2 regulatory region, indicating that Fur repression is exerted by direct binding to the promoter region. In summary, we demonstrated that the EHEC lpf2 operon is regulated in response to temperature, pH, bile salts and iron, during the exponential phase of growth, and is controlled by Fur.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Arenas-Hernández MM,Rojas-López M,Medrano-López A,Nuñez-Reza KJ,Puente JL,Martínez-Laguna Y,Torres AGdoi
10.1111/1574-6968.12513subject
Has Abstractpub_date
2014-08-01 00:00:00pages
105-14issue
2eissn
0378-1097issn
1574-6968journal_volume
357pub_type
信件abstract：:Lipoic acid is an essential cofactor for a variety of mitochondrial enzymes. We have characterised a gene from Saccharomyces cerevisiae which appears to encode a protein involved in the attachment of lipoic acid groups to the pyruvate dehydrogenase and glycine decarboxylase complexes. The predicted protein product of ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb10663.x
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abstract：:We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pair...
journal_title：FEMS microbiology letters
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1995.tb07531.x
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(91)90086-p
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1993.tb06140.x
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journal_title：FEMS microbiology letters
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journal_title：FEMS microbiology letters
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fnz094
更新日期：2019-05-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2002.tb11471.x
更新日期：2002-12-17 00:00:00
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journal_title：FEMS microbiology letters
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journal_title：FEMS microbiology letters
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
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journal_title：FEMS microbiology letters
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journal_title：FEMS microbiology letters
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journal_title：FEMS microbiology letters
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
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journal_title：FEMS microbiology letters
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(91)90224-x
更新日期：1991-07-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb10809.x
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1996.tb08134.x
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abstract：:The glt operon of Escherichia coli comprises the structural genes for the glutamate synthase subunits (gltB and gltD) and gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between gltD and gltF contains a weak promoter and a translation initiation site for gltF. The GltF pr...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1999.tb08711.x
更新日期：1999-10-01 00:00:00
