The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus.

Abstract:

:The effects of specific inhibitors of glycoprotein trimming reactions on Junin virus (JV) replication were investigated. Bromoconduritol, an inhibitor of glucosidase II, significantly reduced infective virus production (DE50: 1.1 mM) and viral protein expression. Neither 1-deoxynojirimycin, an inhibitor of both glucosidases I and II, nor 1-deoxymannojirimycin and swainsonine, inhibitors of mannosidase I and II, respectively, showed any activity against JV multiplication. These results are the first evidence that the acquisition of a complex form of the envelope glycoprotein oligosaccharide chains is not essential for JV infectivity. The effect of bromoconduritol was reversible and probably due to the formation of an unstable intermediate oligosaccharide structure which may be more sensitive to degradative proteolysis.

journal_name

FEMS Microbiol Lett

authors

Silber AM,Candurra NA,Damonte EB

doi

10.1111/j.1574-6968.1993.tb06140.x

subject

Has Abstract

pub_date

1993-05-01 00:00:00

pages

39-43

issue

1

eissn

0378-1097

issn

1574-6968

journal_volume

109

pub_type

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