Mechanisms of action of ostrich beta-defensins against Escherichia coli.

abstract：:The adhesion-promoting proteins (APP) (molecular mass approx. 300 kDa), which promote adhesion of Streptococcus mutans JBP (serotype c) to hydroxyapatite, were isolated from human submandibular-sublingual (SMSL) saliva by gel filtration on a Trisacryl GP2000 M column. The effects of hexoses, pentoses, methyl-pentoses,...

journal_title：FEMS microbiology letters

authors： Kishimoto E,Hay DI,Gibbons RJ

更新日期：1991-12-15 00:00:00

abstract：:In spite of being dispensable, O-antigens are believed to facilitate various cellular processes and alter antibiotic sensitivities. Escherichia coli K-12 (CS109) strains are lacking in O-antigens and are reported to be sensitive to antibiotics. To our surprise, E. coli 2443 (expressing O8-antigen) manifested two- to f...

journal_title：FEMS microbiology letters

authors： Sarkar SK,Ghosh AS

更新日期：2008-05-01 00:00:00

abstract：:An analysis of the 2 kb nucleotide sequence including the 5'-flanking region of a cell-adhesion protein-encoding gene (mfbA) isolated from the basidiomycete Lentinus edodes revealed that the promoter region contains a TATA box, a GC box, a CAAT box, a CT-rich sequence element, a TATA box, two CT-rich sequences, and a ...

journal_title：FEMS microbiology letters

authors： Kondoh O,Shishido K

更新日期：1995-08-01 00:00:00

abstract：:Acetone-butanol-ethanol (ABE) fermentation is a metabolic process of clostridia that produces bio-based solvents including butanol. It is enabled by an underlying metabolic reaction network and modulated by cellular gene regulation and environmental cues. Mathematical modeling has served as a valuable strategy to faci...

journal_title：FEMS microbiology letters

authors： Liao C,Seo SO,Lu T

更新日期：2016-05-01 00:00:00

abstract：:The chimeric plasmid pMY10 containing the origin of replication of pAL5000, the origin of replication of pBR322, the origin of transfer of pRK2 and a kanamycin resistance gene was constructed and successfully transferred by conjugation from Escherichia coli harbouring the helper plasmid pRK4.24 into Mycobacterium smeg...

journal_title：FEMS microbiology letters

authors： Lazraq R,Clavel-Sérès S,David HL,Roulland-Dussoix D

更新日期：1990-05-01 00:00:00

abstract：:We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from...

journal_title：FEMS microbiology letters

authors： Nübel U,Schmidt PM,Reiss E,Bier F,Beyer W,Naumann D

更新日期：2004-11-15 00:00:00

abstract：:Marine viruses are the most abundant biological entity in the oceans, the majority of which infect bacteria and are known as bacteriophages. Yet, the bulk of bacteriophages form part of the vast uncultured dark matter of the microbial biosphere. In spite of the paucity of cultured marine bacteriophages, it is known th...

journal_title：FEMS microbiology letters

authors： Perez Sepulveda B,Redgwell T,Rihtman B,Pitt F,Scanlan DJ,Millard A

更新日期：2016-08-01 00:00:00

abstract：:The Mycobacterium tuberculosis murG gene, Rv2153, was expressed in Escherichia coli murG(Ts) strain OV58 on a plasmid under the control of the arabinose-inducible araBAD promoter. Mycobacterium tuberculosis murG rescued the growth of E. coli murG(Ts) at the nonpermissive temperature: transformants were only obtained i...

journal_title：FEMS microbiology letters

authors： Jha RK,Katagihallimath N,Hota SK,Das KS,de Sousa SM

更新日期：2012-01-01 00:00:00

abstract：:The purpose of this study was to generate highly specific serological reagents for the quantitative identification of Actinomyces naeslundii in clinical samples, in particular dental plaque. Balb/c mice were immunized with pasteurized human A. naeslundii strains representing different genospecies and serotypes. Ten hy...

journal_title：FEMS microbiology letters

authors： Thurnheer T,Guggenheim B,Gmür R

更新日期：1997-05-15 00:00:00

abstract：:Biotype, genome, protein and plasmid profile diversity amongst 40 epidemiologically unrelated strains of Helicobacter pylori was studied. Strains were API Zym biotypes II, III and IV but most (87%) were biotype II. Four subsets of strains were defined on a combination of motility (56% positive) and cytotoxin productio...

journal_title：FEMS microbiology letters

authors： Owen RJ,Bickley J,Moreno M,Costas M,Morgan DR

更新日期：1991-04-15 00:00:00

abstract：:The genus Phytomonas is responsible for many diseases in different crop plant species. The finding that chitin is an exposed cell surface polysaccharide in Phytomonas françai and the observation that chitinases can inhibit fungal growth raises expectations about the potential effect of plant chitinases on the P. franç...

journal_title：FEMS microbiology letters

authors： Gomes Rocha GC,Nicolich R,Romeiro A,Margis-Pinheiro M,Attias M,Alves-Ferreira M

更新日期：2003-09-12 00:00:00

abstract：:Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y. pestis genome databases revealed the presence of n...

journal_title：FEMS microbiology letters

authors： Bobrov AG,Perry RD

更新日期：2006-02-01 00:00:00

abstract：:Most Moraxella catarrhalis isolates express a highly-conserved outer membrane protein of 453 residues designated OMPCD, which has been previously shown to mediate binding to A549 human lung cells. Here, it is reported that two distinct domains of the M. catarrhalis strain O35E OMPCD protein specify adherence. Truncate...

journal_title：FEMS microbiology letters

authors： Akimana C,Lafontaine ER

更新日期：2007-06-01 00:00:00

abstract：:Legionella pneumophila, an intracellular parasite of macrophages and protozoa, requires iron for extra- and intracellular growth. In a new screen of a mutant library of L. pneumophila for strains defective for growth on agar media lacking supplemental iron, seven mutants were obtained. All of the mutants had a disrupt...

journal_title：FEMS microbiology letters

authors： Naylor J,Cianciotto NP

更新日期：2004-12-15 00:00:00

abstract：:The flagellins of Methanospirillum hungatei strains JF1 and GP1, Methanococcus deltae, and Methanothermus fervidus are glycosylated. Isolated flagellar filaments from these organisms are dissociated by low concentrations (0.5% (v/v)) of Triton X-100. Flagellar filaments from other methanogens (Methanococcus voltae, Me...

journal_title：FEMS microbiology letters

authors： Faguy DM,Koval SF,Jarrell KF

更新日期：1992-01-01 00:00:00

abstract：:Enterococcal surface protein (Esp) is a cell wall-associated protein of Enterococcus faecalis that has been identified as a potential virulence factor. We used a mouse model to examine whether Esp facilitates intestinal colonization or translocation of E. faecalis to mesenteric lymph nodes. After clindamycin treatment...

journal_title：FEMS microbiology letters

authors： Pultz NJ,Shankar N,Baghdayan AS,Donskey CJ

更新日期：2005-01-15 00:00:00

abstract：:A Xoo recA insertion inactivation mutant was constructed. The mutant, lacking RecA, showed increased sensitivity towards mutagen killing. This phenotype could be complemented by a cloned, functional recA. Unlike other bacteria, both the recA mutant and the parental strain had similar level of resistance to H2O2 killin...

journal_title：FEMS microbiology letters

authors： Mongkolsuk S,Rabibhadana S,Sukchavalit R,Vaughn G

更新日期：1998-12-15 00:00:00

abstract：:Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall-degra...

journal_title：FEMS microbiology letters

authors： Debeire P,Delalande F,Habrylo O,Jeltsch JM,Van Dorsselaer A,Phalip V

更新日期：2014-06-01 00:00:00

abstract：:The effectiveness of maintaining prokaryotic RNA in Synechococcus and Pseudomonas cells, fixed in 96% ethanol, 4% paraformaldehyde, or suspended in RNAlater, and held in cold storage for 3 months was compared. Fluorometric determination of the RNA extracted from Synechococcus and Pseudomonas cells indicated that the c...

journal_title：FEMS microbiology letters

authors： Bachoon DS,Chen F,Hodson RE

更新日期：2001-07-24 00:00:00

abstract：:Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus. We performed Southern blot hybridization analysis to examine the distribution of Vf33-related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1...

journal_title：FEMS microbiology letters

authors： Chang B,Yoshida S,Miyamoto H,Ogawa M,Horikawa K,Ogata K,Nishibuchi M,Taniguchi H

更新日期：2000-11-15 00:00:00

abstract：:Roundup Ready (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of ...

journal_title：FEMS microbiology letters

authors： Levy-Booth DJ,Gulden RH,Campbell RG,Powell JR,Klironomos JN,Pauls KP,Swanton CJ,Trevors JT,Dunfield KE

更新日期：2009-02-01 00:00:00

abstract：:The replication region of plasmid pRA2 from Pseudomonas alcaligenes NCIB 9867 (strain P25X) was localized within a 5.9-kbp DNA fragment and its sequence was determined. An interesting feature of the sequence is the presence of a 1.3-kbp region containing seven, highly conserved, direct repeats of 72 bp in length. The ...

journal_title：FEMS microbiology letters

authors： Kwong SM,Yeo CC,Chuah D,Poh CL

更新日期：1998-01-15 00:00:00

abstract：:Interest in endophytes as natural sources for new medicines was inspired by the discovery of paclitaxel-producing endophytic fungi. This study investigated the anti-cancer activity of extracts of endophytes isolated from two Australian plants, Eremophila longifolia (EL) and Eremophila maculata (EM). Endophytes were is...

journal_title：FEMS microbiology letters

authors： Zaferanloo B,Pepper SA,Coulthard SA,Redfern CPF,Palombo EA

更新日期：2018-05-01 00:00:00

abstract：:This study describes how bkaR, a highly conserved mycobacterial TetR-like transcriptional repressor, regulates a number of nearby genes that have associations with branched-chain keto-acid metabolism. bkaR (MSMEG_4718) was deleted from the nonpathogenic species Mycobacterium smegmatis, and changes in global gene expre...

journal_title：FEMS microbiology letters

authors： Balhana RJ,Swanston SN,Coade S,Withers M,Sikder MH,Stoker NG,Kendall SL

更新日期：2013-08-01 00:00:00

abstract：:The enzymic interconversion of Escherichia coli isocitrate dehydrogenase (ICDH) between the catalytically active and inactive forms is mediated through the activities of ICDH-kinase/phosphatase in response to changes in the metabolic environment. In this study, the use of mutant strains devoid of isocitrate lyase (ace...

journal_title：FEMS microbiology letters

authors： el-Mansi EM

更新日期：1998-09-15 00:00:00

abstract：:The response regulator proteins of two-component systems mediate many adaptations of bacteria to their ever-changing environment. Most response regulators are transcription factors that alter the level of transcription of specific sets of genes. Activation of response regulators requires their phosphorylation on a con...

journal_title：FEMS microbiology letters

authors： Ladds JC,Muchová K,Blaskovic D,Lewis RJ,Brannigan JA,Wilkinson AJ,Barák I

更新日期：2003-06-27 00:00:00

abstract：:Ceramide mono (CMH) or dihexoside (CDH) fractions from Trypanosoma cruzi (Dm28c clone) were identified as glucosyl and lactosylceramides containing non-hydroxylated fatty acids. The di-glycosylated form was much more efficiently recognized by sera from T. cruzi-immunized rabbits, indicating that glycosylation influenc...

journal_title：FEMS microbiology letters

authors： Villas-Boas MH,Wait R,Silva RB,Rodrigues ML,Barreto-Bergter E

更新日期：2005-03-01 00:00:00

abstract：:Bacteriophage M102 is a virulent siphophage that propagates in some serotype c Streptococcus mutans strains, but not in S. mutans of serotype e, f or k. The serotype of S. mutans is determined by the glucose side chain of rhamnose-glucose polysaccharide (RGP). Because the first step in the bacteriophage infection proc...

journal_title：FEMS microbiology letters

authors： Shibata Y,Yamashita Y,van der Ploeg JR

更新日期：2009-05-01 00:00:00

abstract：:The sequences of the epidermolytic toxins and V8 serine proteinase share about 25% identity, including the catalytic triad at the proteinase active centre. Here we have altered the putative ETA active-site serine-195 to glycine by site-directed mutagenesis. No epidermolytic activity was detected when up to 100-fold gr...

journal_title：FEMS microbiology letters

authors： Redpath MB,Foster TJ,Bailey CJ

更新日期：1991-06-15 00:00:00

abstract：:Strains of the filamentous gliding bacterium Vitreoscilla, LB13 and C1, are shown to be highly sensitive to UV-A (320-400 nm), with an LD50 of less than 20 kJ m(-2). Vitreoscilla LB13 can be protected from UV-A by including superoxide dismutase and catalase, separately or in combination, during the exposure, indicatin...

journal_title：FEMS microbiology letters

authors： Biggs LF,Moody AJ

更新日期：2003-07-15 00:00:00