Oligonucleotide microarray for identification of Bacillus anthracis based on intergenic transcribed spacers in ribosomal DNA.

abstract：:Carboxy (C)-terminal processing proteases (CTP) are a relatively new group of serine proteases. Found in a broad range of organisms - bacteria, archaea, algae, plants and animals - these proteases are involved in the C-terminal processing of proteins. In comparison with amino-terminal processing of bacterial proteins,...

journal_title：FEMS microbiology letters

authors： Hoge R,Laschinski M,Jaeger KE,Wilhelm S,Rosenau F

更新日期：2011-03-01 00:00:00

abstract：:Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump ...

journal_title：FEMS microbiology letters

authors： Yoneda K,Chikumi H,Murata T,Gotoh N,Yamamoto H,Fujiwara H,Nishino T,Shimizu E

更新日期：2005-02-01 00:00:00

abstract：:We have studied the single rRNA gene cluster from the Archaeon, Pyrococcus furiosus. This isolate grows optimally at 100 degrees C and is thus a hyperthermophile. In P. furiosus, transcription of 16S rRNA is subject to regulation over a 7.5-fold range in response to a 20-fold increase in growth rate. The single cluste...

journal_title：FEMS microbiology letters

authors： DiRuggiero J,Achenbach LA,Brown SH,Kelly RM,Robb FT

更新日期：1993-08-01 00:00:00

abstract：:Acinetobacter baumanii, which may be found in water, is an important emerging hospital-acquired pathogen. Free-living amoebae can be recovered from the same water networks, and it has been shown that these protozoa may support the growth of other bacteria. In this paper, we have studied potential relationships between...

journal_title：FEMS microbiology letters

authors： Cateau E,Verdon J,Fernandez B,Hechard Y,Rodier MH

更新日期：2011-06-01 00:00:00

abstract：:Enterobacter cloacae CHE, a clinical strain with overproduced cephalosporinase was found to be highly resistant to the new cephalosporins, cefepime and cefpirome (MICs> or =128 microg ml(-1)). The strain was isolated from a child previously treated with cefepime. The catalytic efficiency of the purified enzyme with th...

journal_title：FEMS microbiology letters

authors： Barnaud G,Labia R,Raskine L,Sanson-Le Pors MJ,Philippon A,Arlet G

更新日期：2001-02-20 00:00:00

abstract：:We have determined the genetic basis of minocycline resistance in a strain of Streptococcus infantis isolated from a healthy human oral cavity. We demonstrate that tet(S), identical to tet(S) found on the enterococcal conjugative transposon Tn6000, is responsible for the observed resistance. The gene is located on a s...

journal_title：FEMS microbiology letters

authors： Ciric L,Brouwer MS,Mullany P,Roberts AP

更新日期：2014-04-01 00:00:00

abstract：:Stability of the membrane-bound and purified H+-translocating NADH:ubiquinone oxidoreductase, Complex I, was studied. The loss of the enzyme activity is strongly increased by alkaline pH and dilution of the sample. Complex I inactivation is prevented specifically by a low concentration of Ca2+ and/or an intracellular ...

journal_title：FEMS microbiology letters

authors： Belevich G,Belevich N,Knuuti J,Verkhovskaya M

更新日期：2018-06-01 00:00:00

abstract：:Three clinical isolates of Salmonella enterica recovered between 2000 and 2003 in France and Senegal were found to produce extended-spectrum beta-lactamase CTX-M-15. The two isolates from Senegal were recovered from stool of a hospitalized patient with gastroenteritis in 2000 and from an urine specimen of an out-patie...

journal_title：FEMS microbiology letters

authors： Weill FX,Perrier-Gros-Claude JD,Demartin M,Coignard S,Grimont PA

更新日期：2004-09-15 00:00:00

abstract：:In order to characterize the metabolites produced in vivo by biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase, the first two enzymes of the (polychloro)biphenyl catabolic pathway encoded by the bph locus of Pseudomonas sp. LB400, recombinant E. coli strains expressing the respective genes were c...

journal_title：FEMS microbiology letters

authors： Seeger M,Timmis KN,Hofer B

更新日期：1995-11-15 00:00:00

abstract：:Integrons are bacterial genetic elements capable of capturing and expressing potentially adaptive genetic material. Class 1 integrons constitute the most intensely studied group of these elements to date, mainly due to their well-established role in the acquisition and dissemination of antibiotic resistance genes in c...

journal_title：FEMS microbiology letters

authors： Hardwick SA,Stokes HW,Findlay S,Taylor M,Gillings MR

更新日期：2008-01-01 00:00:00

abstract：:A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 other bacterial species. A detection limit of 1 cell equivalent wa...

journal_title：FEMS microbiology letters

authors： O'Grady J,Lacey K,Glynn B,Smith TJ,Barry T,Maher M

更新日期：2009-12-01 00:00:00

abstract：:Recent reports have indicated that cholesterol plays a crucial role during the uptake of mycobacteria by macrophages. However, the significance of cholesterol modification enzymes encoded by Mycobacterium tuberculosis for bacterial pathogenicity remains unknown. Here, the authors explored whether the well-known choles...

journal_title：FEMS microbiology letters

authors： Brzostek A,Dziadek B,Rumijowska-Galewicz A,Pawelczyk J,Dziadek J

更新日期：2007-10-01 00:00:00

abstract：:Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allo...

journal_title：FEMS microbiology letters

authors： Moravek M,Wegscheider M,Schulz A,Dietrich R,Bürk C,Märtlbauer E

更新日期：2004-09-01 00:00:00

abstract：:Hopanoid fingerprints allowed to differentiate bacteria formerly connected to the genus Pseudomonas. Whereas all strains related to Pseudomonas and Ralstonia were devoid of any detectable hopanoid, these pentacyclic triterpenoids were found in the Burkholderia species and in related soil isolates, which contained as m...

journal_title：FEMS microbiology letters

authors： Cvejic JH,Putra SR,El-Beltagy A,Hattori R,Hattori T,Rohmer M

更新日期：2000-02-15 00:00:00

abstract：:The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble ...

journal_title：FEMS microbiology letters

authors： Sándor E,Pusztahelyi T,Karaffa L,Karányi Z,Pócsi I,Biró S,Szentirmai A,Pócsi I

更新日期：1998-07-15 00:00:00

abstract：:Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in ...

journal_title：FEMS microbiology letters

authors： Bäumchen C,Krings E,Bringer S,Eggeling L,Sahm H

更新日期：2009-01-01 00:00:00

abstract：:The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthet...

journal_title：FEMS microbiology letters

authors： Pissavin C,Hugouvieux-Cotte-Pattat N

更新日期：1997-12-01 00:00:00

abstract：:Three 16S rRNA-targeted oligonucleotide probes, namely, PSMg437 targeting several members of the genus Pseudomonas, Hlm474 targeting several members of the genus Halomonas, and Clw844 targeting several members of the genus Colwellia, were designed. The microbial community structure and nitrogen removal ability of nitr...

journal_title：FEMS microbiology letters

authors： Yoshie S,Noda N,Tsuneda S,Hirata A,Inamori Y

更新日期：2004-06-01 00:00:00

abstract：:Riemerella anatipestifer is the causative agent of polyserositis of ducks and geese. We have previously reported that a 3.9-kb plasmid, pCFC1, carries protein genes (vapD1 and vapD2) that are similar to virulence-associated genes of other bacteria. In the present study, we report the complete sequence of a second plas...

journal_title：FEMS microbiology letters

authors： Weng S,Lin W,Chang Y,Chang C

更新日期：1999-10-01 00:00:00

abstract：:Clostridium difficile is the causative agent for pseudomembranous colitis in humans. Toxic strains of C. difficile produce two toxins, toxin A and toxin B. A reliable and definitive method of typing the toxic strains of C. difficile is needed since nosocomial cross infection is a primary concern in hospitals and other...

journal_title：FEMS microbiology letters

authors： McMillin DE,Muldrow LL

更新日期：1992-04-01 00:00:00

abstract：:Clostridium thermocellum, a Gram-positive, thermophilic anaerobe produces a highly active cellulase system. This system, termed the cellulosome, is a complex composed of at least 14-18 different types of components organized around a large, cellulose-binding protein. Combining recombinant DNA technology and protein bi...

journal_title：FEMS microbiology letters

authors： Béguin P,Millet J,Aubert JP

更新日期：1992-12-15 00:00:00

abstract：:The impact of four electron acceptors on hydrocarbon-induced methanogenesis was studied. Methanogenesis from residual hydrocarbons may enhance the exploitation of oil reservoirs and may improve bioremediation. The conditions to drive the rate-limiting first hydrocarbon-oxidizing steps for the conversion of hydrocarbon...

journal_title：FEMS microbiology letters

authors： Siegert M,Cichocka D,Herrmann S,Gründger F,Feisthauer S,Richnow HH,Springael D,Krüger M

更新日期：2011-02-01 00:00:00

abstract：:Escherichia coli heat stable enterotoxin (STa) binds to isolated rat intestinal epithelial cells and triggers a cascade reaction including increase of intracellular calcium levels ([Ca(2+)](i)) and membrane bound protein kinase C (PKC) activity. In response to STa, the cytosolic PKC activity falls from 110 to 35 nmol ...

journal_title：FEMS microbiology letters

authors： Ganguly U,Chaudhury AG,Basu A,Sen PC

更新日期：2001-10-16 00:00:00

abstract：:Cyanobacterial natural products offer new possibilities for drugs and lead compounds but many factors can inhibit the production of sufficient yields for pharmaceutical processes. While Escherichia coli and Streptomyces sp. have been used as heterologous expression hosts to produce cyanobacterial natural products, the...

journal_title：FEMS microbiology letters

authors： Wells KN,Videau P,Nelson D,Eiting JE,Philmus B

更新日期：2018-08-01 00:00:00

abstract：:The argH gene encoding argininosuccinate lyase (ASL) of Methanococcus maripaludis was cloned on a 4.7-kb HindIII genomic fragment. The gene is preceded by a short open reading frame (ORF149), which encodes a polypeptide with an unknown function. The two genes are co-transcribed. The ASL of M. maripaludis shares a high...

journal_title：FEMS microbiology letters

authors： Cohen-Kupiec R,Kupiec M,Sandbeck K,Leigh JA

更新日期：1999-04-01 00:00:00

abstract：:The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [14C]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [14C]alanine was further actively metabolised to g...

journal_title：FEMS microbiology letters

authors： Chalot M,Brun A,Finlay RD,Söderström B

更新日期：1994-08-01 00:00:00

abstract：:In the present study, the homology relationships among fragments amplified by ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Extragenic Palindromic) primers in Drechslera avenae and Stemphylium solani causing leaf blotch and black spot of oats and leaf blight of cotton, respectively, were i...

journal_title：FEMS microbiology letters

authors： Mehta A,Mehta YR,Rosato YB

更新日期：2002-05-21 00:00:00

abstract：:Testosterone and 4-androstene-3,17-dione (androstenedione) binding proteins were found in the cytosol of Cochliobolus lunatus, a filamentous fungus. The maximal androgen binding occurred by 120 min at 0 degrees C. Androgen binding proteins were degraded by protease treatment and at high temperature. The mean Kd determ...

journal_title：FEMS microbiology letters

authors： Kastelic-Suhadolc T,Lenasi H

更新日期：1993-03-15 00:00:00

abstract：:The expression of nifA-, niH- and nifB-lacZ fusions was examined in different mutants of Azospirillum brasilense. Mutations in nifA, glnA and glnB severely impaired the expression of nifH- and nifB-lacZ fusions. By contrast, a nifA-lacZ fusion was not affected in a nifA or a glnB background and was only partially impa...

journal_title：FEMS microbiology letters

authors： Liang YY,de Zamaroczy M,Arsène F,Paquelin A,Elmerich C

更新日期：1992-12-15 00:00:00

abstract：:Growth of Prevotella ruminicola strains B(1)4 (subsp. brevis) and D31d (subsp. ruminicola), was inhibited by protamine, a polycationic, low molecular mass protein. Results showed that protamine has a bacteriocidal effect when present in concentrations exceeding 30 micrograms ml-1. Protamine exerted its toxic effects b...

journal_title：FEMS microbiology letters

authors： Madeira HM,Morrison M

更新日期：1997-05-01 00:00:00