Abstract:
:Ceramide mono (CMH) or dihexoside (CDH) fractions from Trypanosoma cruzi (Dm28c clone) were identified as glucosyl and lactosylceramides containing non-hydroxylated fatty acids. The di-glycosylated form was much more efficiently recognized by sera from T. cruzi-immunized rabbits, indicating that glycosylation influences antigenicity. Fatty acid hydroxylation was also a determinant of serological reactivity, since an alpha-hydroxylated CMH, only present at the Y clone, was recognized by the hyperimmune sera. In summary, these data indicate that T. cruzi CMHs with non-hydroxylated fatty acids are unable to induce antibody responses in animal hosts, which is reverted by the addition of a sugar residue or an alpha-hydroxyl group.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Villas-Boas MH,Wait R,Silva RB,Rodrigues ML,Barreto-Bergter Edoi
10.1016/j.femsle.2005.01.024keywords:
subject
Has Abstractpub_date
2005-03-01 00:00:00pages
47-52issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(05)00038-8journal_volume
244pub_type
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