Ceramide glycosylation and fatty acid hydroxylation influence serological reactivity in Trypanosoma cruzi glycosphingolipids.

Abstract:

:Ceramide mono (CMH) or dihexoside (CDH) fractions from Trypanosoma cruzi (Dm28c clone) were identified as glucosyl and lactosylceramides containing non-hydroxylated fatty acids. The di-glycosylated form was much more efficiently recognized by sera from T. cruzi-immunized rabbits, indicating that glycosylation influences antigenicity. Fatty acid hydroxylation was also a determinant of serological reactivity, since an alpha-hydroxylated CMH, only present at the Y clone, was recognized by the hyperimmune sera. In summary, these data indicate that T. cruzi CMHs with non-hydroxylated fatty acids are unable to induce antibody responses in animal hosts, which is reverted by the addition of a sugar residue or an alpha-hydroxyl group.

journal_name

FEMS Microbiol Lett

authors

Villas-Boas MH,Wait R,Silva RB,Rodrigues ML,Barreto-Bergter E

doi

10.1016/j.femsle.2005.01.024

keywords:

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

47-52

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(05)00038-8

journal_volume

244

pub_type

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