Importance of the galE gene on the virulence of Pasteurella multocida.

abstract：:A novel strain of Rhizopus oryzae WPG secretes a noninduced lipase (ROLw) in the culture medium; purified ROLw is a protein of 29 kDa, the 45 N-terminal amino acid residues were sequenced, this sequence is very homologous to Rhizopus delemar lipase (RDL), Rhizopus niveus lipase (RNL) and R. oryzae lipase (ROL29) seque...

journal_title：FEMS microbiology letters

authors： Salah RB,Mosbah H,Fendri A,Gargouri A,Gargouri Y,Mejdoub H

更新日期：2006-07-01 00:00:00

abstract：:Strains of Salmonella enteritidis expressed a novel porin with a subunit size of 35.5 kDa as shown by SDS-PAGE. This protein was expressed as a major outer membrane protein (MOMP) when grown on nutrient agar, McConkey agar or blood agar, or in Tris-succinate medium; but was only produced in trace amounts when strains ...

journal_title：FEMS microbiology letters

authors： Chart H,Frost JA,Rowe B

更新日期：1993-05-15 00:00:00

abstract：:Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. ce...

journal_title：FEMS microbiology letters

authors： Hyeon JE,Yu KO,Suh DJ,Suh YW,Lee SE,Lee J,Han SO

更新日期：2010-09-01 00:00:00

abstract：:We characterized Yersinia enterocolitica and Yersinia pseudotuberculosis insertion sequences related to insertion sequence 1541, recently identified in Yersinia pestis. For each of the two species, two insertion sequence copies were cloned and sequenced. Genetic elements from Y. pseudotuberculosis were almost identica...

journal_title：FEMS microbiology letters

authors： Devalckenaere A,Odaert M,Trieu-Cuot P,Simonet M

更新日期：1999-07-01 00:00:00

abstract：:A different mutation (SMR1B) to SMR1-410 (ilv2), which determines resistance to sulfometuron methyl in Saccharomyces cerevisiae, was cloned by PCR. Sequence analysis indicated a C to T change at position 575 of the ILV2 coding sequence, which results in a proline transition to leucine at position 192. Similarly to SMR...

journal_title：FEMS microbiology letters

authors： Xie Q,Jiménez A

更新日期：1996-04-01 00:00:00

abstract：:The application of series of high intensity electric pulses to a yeast suspension provoked a considerable release of some cytoplasmic proteins, glutathione reductase, 3-phosphoglycerate kinase and alcohol dehydrogenase. A maximal yield was achieved 3-8 h after pulsation. The electro-induced protein efflux was accelera...

journal_title：FEMS microbiology letters

authors： Ganeva V,Galutzov B

更新日期：1999-05-15 00:00:00

abstract：:The est gene encoding an esterase from Acinetobacter lwoffii RAG-1 was cloned into E. coli under the control of the PL promoter of the phage lambda. The N-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence. Antibodies prepared...

journal_title：FEMS microbiology letters

authors： Alon RN,Gutnick DL

更新日期：1993-09-15 00:00:00

abstract：:Both form I and II ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes were detected in Thiobacillus intermedius by heterologous hybridization using specific probes from Anacystis nidulans and Rhodobacter sphaeroides, respectively. However, only the previously reported form I enzyme could be demonstrated i...

journal_title：FEMS microbiology letters

authors： Stoner MT,Shively JM

更新日期：1993-03-01 00:00:00

abstract：:Eikenella corrodens 33EK(L), a clinical isolate, was assayed for its ability to utilise amino acids as substrates in the reduction of nitrate to nitrite. The metabolism of proline, glutamate, serine and glutamine was found to result in relatively high rates of nitrate reduction. The ability of cells to metabolise thes...

journal_title：FEMS microbiology letters

authors： Gully NJ,Rogers AH

更新日期：1996-06-01 00:00:00

abstract：:Corynebacterium diphtheriae, generally considered an extracellular coloniser, was evaluated for its ability to enter and survive within HEp-2 monolayers by gentamicin protection assay. Intracellular viability of HC01 strain, isolated from endocarditis, was more expressive (2.59%) than observed in 241 (0.21%) and CDC-E...

journal_title：FEMS microbiology letters

authors： Hirata R,Napoleão F,Monteiro-Leal LH,Andrade AF,Nagao PE,Formiga LC,Fonseca LS,Mattos-Guaraldi AL

更新日期：2002-09-24 00:00:00

abstract：:Fourteen strains representing 11 Caragana-nodulating Mesorhizobium genomic species were identified as representing Mesorhizobium amorphae, Mesorhizobium huakuii, Mesorhizobium septentrionale and groups related to Mesorhizobium plurifarium, Mesorhizobium temperatum, Mesorhizobium tianshanense and Mesorhizobium mediterr...

journal_title：FEMS microbiology letters

authors： Chen WF,Guan SH,Zhao CT,Yan XR,Man CX,Wang ET,Chen WX

更新日期：2008-06-01 00:00:00

abstract：:Chlamydophila pneumoniae infection is associated with a range of diseases including pneumonia, asthma and heart disease. Although an obligate intracellular pathogen, high levels of antigen-specific antibody are induced and serology is frequently used to diagnose these infections. Proteins targeted by the humoral respo...

journal_title：FEMS microbiology letters

authors： Cunningham AF,Ward ME

更新日期：2003-10-10 00:00:00

abstract：:The filamentous ascomycete Botrytis cinerea is one of the most studied models for understanding the necrotrophic behaviour of phytopathogenic fungi. The genomes of two strains of B. cinerea have been sequenced (B05.10 and T4), which may contribute to elucidating the virulence polymorphism in this fungus. In this study...

journal_title：FEMS microbiology letters

authors： Choquer M,Robin G,Le Pêcheur P,Giraud C,Levis C,Viaud M

更新日期：2008-12-01 00:00:00

abstract：:Alternaria brassicicola is a pathogen that penetrates directly through the host cuticle thanks to several serine esterases, according to our findings. Among these, an 80-kDa lipase (E.C 3.1.1. 3) was detected by SDS-PAGE and immunoblotting in the water washings of ungerminated spores. The purified lipase cross-reacted...

journal_title：FEMS microbiology letters

authors： Berto P,Comménil P,Belingheri L,Dehorter B

更新日期：1999-11-15 00:00:00

abstract：:A Gram-negative, nonmotile and rod-shaped bacterial strain was isolated from the rhizosphere of Platycodon grandiflorum in a study of bacterial diversity, and its taxonomic position was investigated by a genotypic and phenotypic analysis. This isolate, designated as DR-f4, grew at 4-30 degrees C (optimally at 20-25 de...

journal_title：FEMS microbiology letters

authors： Kim BC,Lee KH,Kim MN,Lee J,Shin KS

更新日期：2010-08-01 00:00:00

abstract：:A novel alphaproteobacterium, designated KMU-156T, was isolated from seawater collected on the coast of Jeju Island, Republic of Korea, and its phylogenetic position was determined using a polyphasic taxonomic approach. Strain KMU-156T was Gram-stain-negative, strictly aerobic, apricot-colored, rod-shaped, non-motile ...

journal_title：FEMS microbiology letters

authors： Yoon J

更新日期：2019-08-01 00:00:00

abstract：:Two plasmids, pEM4 (IncFI) determining virulence and pEM6 (IncI) determining colicine I production and resistance to tetracycline, have been found in a pathogenic strain of Citrobacter freundii. Even though pEM4 and pEM6 plasmids are conjugative and transfer themselves very efficiently during conjugation, a high cotra...

journal_title：FEMS microbiology letters

authors： Morzejko E,Panek E,Cebrat S

更新日期：1990-03-01 00:00:00

abstract：:A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. Sequence analysis revealed that this cluster contains the genes for the scaffolding protein CipA, the processive endocellulase Cel48A, several endoglucanases of families 5 and 9, the mannanase Man5...

journal_title：FEMS microbiology letters

authors： Sabathé F,Bélaïch A,Soucaille P

更新日期：2002-11-19 00:00:00

abstract：:Lipoic acid is an essential cofactor for a variety of mitochondrial enzymes. We have characterised a gene from Saccharomyces cerevisiae which appears to encode a protein involved in the attachment of lipoic acid groups to the pyruvate dehydrogenase and glycine decarboxylase complexes. The predicted protein product of ...

journal_title：FEMS microbiology letters

authors： Marvin ME,Williams PH,Cashmore AM

更新日期：2001-05-15 00:00:00

abstract：:The precise molecular mechanism of Staphylococcus aureus beta-toxin inactivation by the serotype F triple-converting phage phi 42, phi A1 and phi A3 was investigated. Sequence analysis of the phi 42 (attP) and Staphylococcus aureus (attB) attachment sites and the left (attL) and right (attR) chromosomal/bacteriophage ...

journal_title：FEMS microbiology letters

authors： Carroll JD,Cafferkey MT,Coleman DC

更新日期：1993-01-15 00:00:00

abstract：:E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium L...

journal_title：FEMS microbiology letters

authors： Cortes-Perez NG,Bermúdez-Humarán LG,Le Loir Y,Rodriguez-Padilla C,Gruss A,Saucedo-Cárdenas O,Langella P,Montes-de-Oca-Luna R

更新日期：2003-12-05 00:00:00

abstract：:The prodrug activation system formed by the E. coli codA gene encoding cytosine deaminase (CD) and 5-fluorocytosine (5-FC) developed for selective cancer chemotherapy suffers from a sensitivity limitation in many tumour cells. In an attempt to improve the CD/5-FC suicide association, we combined the E. coli upp gene e...

journal_title：FEMS microbiology letters

authors： Tiraby M,Cazaux C,Baron M,Drocourt D,Reynes JP,Tiraby G

更新日期：1998-10-01 00:00:00

abstract：:Incubation of Mycobacterium tuberculosis and M smegmatis cells with the sugar components of their surface-exposed glycans demonstrated that D-arabinose but not alpha-D-glucose or D-mannose, led to the dispersion of the large clumps formed by the bacilli in stationary liquid cultures. These results confirm the presence...

journal_title：FEMS microbiology letters

authors： Anton V,Rougé P,Daffé M

更新日期：1996-11-01 00:00:00

abstract：:Three different whole cell biosensor constructs were made by fusing the mercury inducible promoter, P(mer), and its regulatory gene, merR, from transposon Tn21 with reporter genes luxCDABE, lacZYA, or gfp. In Escherichia coli these biosensor constructs responded to low levels of mercury by producing light, beta-galact...

journal_title：FEMS microbiology letters

authors： Hansen LH,Sørensen SJ

更新日期：2000-12-01 00:00:00

abstract：:Paracoccus pantotrophus grown anaerobically under denitrifying conditions expressed similar levels of the periplasmic nitrate reductase (NAP) when cultured in molybdate- or tungstate-containing media. A native PAGE gel stained for nitrate reductase activity revealed that only NapA from molybdate-grown cells displayed ...

journal_title：FEMS microbiology letters

authors： Gates AJ,Hughes RO,Sharp SR,Millington PD,Nilavongse A,Cole JA,Leach ER,Jepson B,Richardson DJ,Butler CS

更新日期：2003-03-28 00:00:00

abstract：:The frequency of spontaneous as well as induced reversions of auxotrophic mutations in Escherichia coli AB1157 and its DeltadnaK and DeltadnaKdnaJ derivatives was estimated. The obtained results demonstrate that both mutants tested are characterized by elevated frequency of spontaneous reversions compared to their AB1...

journal_title：FEMS microbiology letters

authors： Grudniak AM,Kuć M,Wolska KI

更新日期：2005-01-15 00:00:00

abstract：:The technique based on fluorescein-linked lectins used to determine the cell wall structure of anaerobic rumen fungi belonging to genera: Neocallimastix, Piromonas and Sphaeromonas, appears to be an interesting tool for distinguishing between strains. Furthermore this technique shows differences of cell wall compositi...

journal_title：FEMS microbiology letters

authors： Guillot J,Breton A,Damez M,Dusser M,Gaillard-Martinie B,Millet L

更新日期：1990-01-15 00:00:00

abstract：:CRISPR-Cas systems provide adaptive immunity against mobile genetic elements, but employment of this resistance mechanism is often reported with a fitness cost for the host. Whether or not CRISPR-Cas systems are important barriers for the horizontal spread of conjugative plasmids, which play a crucial role in the spre...

journal_title：FEMS microbiology letters

authors： van Sluijs L,van Houte S,van der Oost J,Brouns SJ,Buckling A,Westra ER

更新日期：2019-03-01 00:00:00

abstract：:It was speculated that the increase of the UDP-GlcNAc pool observed with chloramphenicol can modulate the residual PEP:UDP-GlcNAc-enolpyruvate activity of fosfomycin-treated cells. This provided an explanation on how chloramphenicol can insure the formation of enough UDP-MurNAc-pentapeptide to sustain peptidoglycan sy...

journal_title：FEMS microbiology letters

authors： Mengin-Lecreulx D,van Heijenoort J

更新日期：1990-01-01 00:00:00

abstract：:Two variants have been isolated from the wild-type Azospirillum lipoferum strain 4B. The first variant, 4V(I), spontaneously emerged from the wild-type at frequencies in the order of 10(-4) to 10(-3) per cell generation. Compared to the wild-type, the 4V(I) variant gained (production of a carotenoid-like pigment, assi...

journal_title：FEMS microbiology letters

authors： Alexandre G,Bally R

更新日期：1999-05-15 00:00:00