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Conjugational cotransfer of IncFI and IncI conjugative plasmids forming aggregate in a pathogenic Citrobacter freundii strain.

Abstract：

:Two plasmids, pEM4 (IncFI) determining virulence and pEM6 (IncI) determining colicine I production and resistance to tetracycline, have been found in a pathogenic strain of Citrobacter freundii. Even though pEM4 and pEM6 plasmids are conjugative and transfer themselves very efficiently during conjugation, a high cotransfer of both plasmids is observed--an unusually high fraction of transconjugants acquiring pEM6 acquires pEM4 and vice versa. The observed cotransfer of these plasmids is connected with their ability to complement their conjugational functions. An insertion mutant of pEM4 with decreased frequency of conjugational transfer has been isolated. This mutant (pEM44) lost its ability to mobilize nonconjugative plasmids. Its transfer is stimulated by pEM6 and it is transferred from (pEM44, pEM6) donors almost exclusively with pEM6 plasmid. The role of cotransfer and stimulation phenomena in spreading of plasmid aggregates in bacterial population is discussed.

journal_name

FEMS Microbiol Lett

journal_title

FEMS microbiology letters

authors

Morzejko E,Panek E,Cebrat S

doi

10.1016/0378-1097(90)90115-7

subject

Has Abstract

pub_date

1990-03-01 00:00:00

pages

7-11

issue

1-2

eissn

0378-1097

issn

1574-6968

journal_volume

56

pub_type

杂志文章

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