Deletion of the C-terminus of polynucleotide phosphorylase increases twitching motility, a virulence characteristic of the anaerobic bacterial pathogen Dichelobacter nodosus.

Abstract:

:The Gram-negative anaerobe Dichelobacter nodosus is the causative agent of footrot in sheep. Different strains of D. nodosus cause disease of differing severities, ranging from benign to virulent. Virulent strains have greater twitching motility and secrete proteases that are more thermostable than those secreted by benign strains. We have identified polynucleotide phosphorylase (PNPase) as a putative virulence regulator and have proposed that PNPase expression is modulated by the adjacent integration of genetic elements. In this study, we compared PNPase activity in three virulent and four benign strains of D. nodosus and found that PNPase activity is lower in virulent strains. We disrupted the pnpA gene in three benign D. nodosus strains and two virulent strains and showed that deletion of the S1 domain of PNPase reduced catalytic activity. In all but one case, deletion of the PNPase S1 domain had no effect on the thermostability of extracellular proteases. However, this deletion resulted in an increase in twitching motility in benign, but not in virulent strains. Reconstruction of the pnpA gene in two mutant benign strains reduced twitching motility to the parental level. These results support the hypothesis that PNPase is a virulence repressor in benign strains of D. nodosus.

journal_name

FEMS Microbiol Lett

authors

Palanisamy SK,Fletcher C,Tanjung L,Katz ME,Cheetham BF

doi

10.1111/j.1574-6968.2009.01831.x

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

39-45

issue

1

eissn

0378-1097

issn

1574-6968

pii

FML1831

journal_volume

302

pub_type

杂志文章
  • Diversity and distribution of hemerythrin-like proteins in prokaryotes.

    abstract::Hemerythrins are oxygen-binding proteins found in the body fluids and tissues of certain invertebrates. Oxygen is bound at a nonheme iron centre consisting of two oxo-bridged iron atoms bound to a characteristic set of conserved histidine: aspartate and glutamate residues with the motifs H-HxxxE-HxxxH-HxxxxD. It has r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2007.01011.x

    authors: French CE,Bell JM,Ward FB

    更新日期:2008-02-01 00:00:00

  • Genetic investigation within Lactococcus garvieae revealed two genomic lineages.

    abstract::The diversity of a collection of 49 Lactococcus garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin, was explored using a molecular polyphasic approach comprising PCR-ribotyping, REP and RAPD-PCR analyses and a multilocus restriction typing (MLRT) carried out on six partial genes (at...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/j.1574-6968.2012.02591.x

    authors: Ferrario C,Ricci G,Borgo F,Rollando A,Fortina MG

    更新日期:2012-07-01 00:00:00

  • Identification Leptospira santarosai serovar shermani specific sequences by suppression subtractive hybridization.

    abstract::In Taiwan, leptospirosis is caused mainly by Leptospira santarosai serovar shermani. Suppression subtractive hybridization was employed to isolate DNA fragments present in pathogenic L. santarosai serovar shermani but absent in non-pathogenic L. biflexa serovar patoc. Analysis of 23 subtracted DNA clones revealed 25 g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.04.024

    authors: Hsieh WJ,Pan MJ

    更新日期:2004-06-01 00:00:00

  • Immunological characterization of the Prochlorothrix hollandica and Prochloron sp. chlorophyll a/b antenna proteins.

    abstract::Polyclonal antibodies were prepared against the major antenna chlorophyll (Chl) a/b-binding protein from the prokaryote Prochlorothrix hollandica (Burger-Wiersma et al. (1986) Nature (Lond.) 320, 262-264). Immunoblotting experiments on Triton X-114 phase-partitioned P. hollandica thylakoids revealed that the antibody ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90176-q

    authors: Bullerjahn GS,Jensen TC,Sherman DM,Sherman LA

    更新日期:1990-01-15 00:00:00

  • Typing Tuber melanosporum and Chinese black truffle species by molecular markers.

    abstract::Morphologically similar species such as Chinese black truffles and Tuber melanosporum were typed by restriction length polymorphism of internal transcribed spacers. This analysis together with sequence comparison revealed the presence of high genetic variability among fruit bodies collected in China. Selection of prim...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb12582.x

    authors: Paolocci F,Rubini A,Granetti B,Arcioni S

    更新日期:1997-08-15 00:00:00

  • Molecular analysis of ancient microbial infections.

    abstract::The detection of ancient microbial DNA offers a new approach for the study of infectious diseases, their occurrence, frequency and host-pathogen interaction in historic times and populations. Moreover, data obtained from skeletal and mummified tissue may represent an important completion of contemporary phylogenetic a...

    journal_title:FEMS microbiology letters

    pub_type: 历史文章,杂志文章,评审

    doi:10.1111/j.1574-6968.2002.tb11298.x

    authors: Zink AR,Reischl U,Wolf H,Nerlich AG

    更新日期:2002-08-06 00:00:00

  • Glucomannan utilization operon of Bacillus subtilis.

    abstract::This study characterizes the glucomannan utilization operon (gmuBACDREFG, formerly ydhMNOPQRST) of Bacillus subtilis. Transcription of the operon is induced by konjac glucomannan and requires the last mannanase gene (gmuG). Cellobiose and mannobiose, possible degradation products of glucomannan by GmuG, are strong ind...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.01018.x

    authors: Sadaie Y,Nakadate H,Fukui R,Yee LM,Asai K

    更新日期:2008-02-01 00:00:00

  • Protein expression by Streptococcus uberis in co-culture with bovine mammary epithelial cells.

    abstract::Three strains of Streptococcus uberis isolated from dairy cows with mastitis were co-cultured with a bovine mammary epithelial cell line (MAC-T) in Dulbecco's modified Eagle's medium without fetal bovine serum. Protein profiles from culture supernatants and bacterial pellets among different treatments were compared by...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13896.x

    authors: Fang W,Luther DA,Oliver SP

    更新日期:1998-09-15 00:00:00

  • A novel thermostable dextranase from a Thermoanaerobacter species cultured from the geothermal waters of the Great Artesian Basin of Australia.

    abstract::A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was pro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(96)00193-0

    authors: Wynter C,Patel BK,Bain P,de Jersey J,Hamilton S,Inkerman PA

    更新日期:1996-07-01 00:00:00

  • Cloning and nucleotide sequence of a gene upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7.

    abstract::A DNA segment located immediately upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7 strain HA1 was cloned and sequenced. This segment contained an open reading frame encoding a predicted protein of 156 amino acids. A database search identified similar open reading frames upstream of the eaeA gene...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07856.x

    authors: Zhao S,Mitchell SE,Meng J,Doyle MP,Kresovich S

    更新日期:1995-11-01 00:00:00

  • Expression and characterization of Aspergillus thermostable phytases in Pichia pastoris.

    abstract::Two thermostable phytases were identified from Thai isolates of Aspergillus japonicus BCC18313 (TR86) and Aspergillus niger BCC18081 (TR170). Both genes of 1404 bp length, coding for putative phytases of 468 amino acid residues, were cloned and transferred into Pichia pastoris. The recombinant phytases, r-PhyA86 and r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01399.x

    authors: Promdonkoy P,Tang K,Sornlake W,Harnpicharnchai P,Kobayashi RS,Ruanglek V,Upathanpreecha T,Vesaratchavest M,Eurwilaichitr L,Tanapongpipat S

    更新日期:2009-01-01 00:00:00

  • Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites.

    abstract::Occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in Japan and Korea. The tet(M) gene was detected in 34 Japanese and Korean isolates, which included Vibrio sp., Lactococcu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.06.026

    authors: Kim SR,Nonaka L,Suzuki S

    更新日期:2004-08-01 00:00:00

  • Solubilization of zinc salts by a bacterium isolated from the air environment of a tannery.

    abstract::Airborne bacteria isolated from a tannery air environment were screened for the property of solubilization of insoluble zinc oxide and zinc phosphate. Out of 10 strains tested, a strain of Pseudomonas aeruginosa (CMG 823) showed the best solubilization and solubilized both zinc oxide and zinc phosphate. Colonies of th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11277.x

    authors: Fasim F,Ahmed N,Parsons R,Gadd GM

    更新日期:2002-07-16 00:00:00

  • Induction of gram-negative bacterial growth by neurochemical containing banana (Musa x paradisiaca) extracts.

    abstract::Bananas contain large quantities of neurochemicals. Extracts from the peel and pulp of bananas in increasing stages of ripening were prepared and evaluated for their ability to modulate the growth of non-pathogenic and pathogenic bacteria. Extracts from the peel, and to a much lesser degree the pulp, increased the gro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb12651.x

    authors: Lyte M

    更新日期:1997-09-15 00:00:00

  • Bacillus and Streptomyces were selected as broad-spectrum antagonists against soilborne pathogens from arid areas in Egypt.

    abstract::Plant protection via disease-suppressive bacteria in desert farming requires specific biological control agents (BCAs) adapted to the unique arid conditions. We performed an ecological study of below-ground communities in desert farm soil and untreated desert soil, and based on these findings, selected antagonists wer...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12089

    authors: Köberl M,Ramadan EM,Adam M,Cardinale M,Hallmann J,Heuer H,Smalla K,Berg G

    更新日期:2013-05-01 00:00:00

  • Sesquiterpene farnesol as a competitive inhibitor of lipase activity of Staphylococcus aureus.

    abstract::Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L(-1)) inhibits this lipase activity on a Mueller-Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00772.x

    authors: Kuroda M,Nagasaki S,Ito R,Ohta T

    更新日期:2007-08-01 00:00:00

  • Current climate for digital game-based learning of science in further and higher education.

    abstract::Digital game-based learning (DGBL) is being used increasingly as an alternative learning tool to teach science in further and higher education. A variety of digital game formats currently exist for science learning, alongside diverse methods for their implementation and evaluation. This paper aims to provide a broad s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fny237

    authors: Brown CL,Comunale MA,Wigdahl B,Urdaneta-Hartmann S

    更新日期:2018-11-01 00:00:00

  • Interaction of dietary resveratrol with animal-associated bacteria.

    abstract::Resveratrol (3,5,4'-trihydroxy-trans-stilbene), an antifungal phytoalexin produced by grapes, peanuts, and Japanese knotweeds, is thought to be a beneficial dietary phytochemical in red wine and grape juice. Information on its antibacterial properties and biotransformation, however, is limited. We surveyed the interac...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01691.x

    authors: Jung CM,Heinze TM,Schnackenberg LK,Mullis LB,Elkins SA,Elkins CA,Steele RS,Sutherland JB

    更新日期:2009-08-01 00:00:00

  • Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean.

    abstract::Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodula...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02280.x

    authors: Althabegoiti MJ,Covelli JM,Pérez-Giménez J,Quelas JI,Mongiardini EJ,López MF,López-García SL,Lodeiro AR

    更新日期:2011-06-01 00:00:00

  • Genomic 3' terminal sequence comparison of three isolates of rabbit haemorrhagic disease virus.

    abstract::Comparison of sequence data is necessary in older to investigate virus origins, identify features common to virulent strains, and characterize genomic organization within virus families. A virulent caliciviral disease of rabbits recently emerged in China. We have sequenced 1100 bases from the 3' ends of two independen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90486-8

    authors: Milton ID,Vlasak R,Nowotny N,Rodak L,Carter MJ

    更新日期:1992-05-15 00:00:00

  • Novel helper phage design: intergenic region affects the assembly of bacteriophages and the size of antibody libraries.

    abstract::Phagemid vectors for display of protein/peptides on the surface of filamentous phage utilize a plasmid genome carrying the phage origin of replication, along with the gene fused to a fragment of gene III. Generation of phage particles displaying the fusion protein also requires superinfection of the host bacterium wit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07375.x

    authors: Dueñas M,Borrebaeck CA

    更新日期:1995-01-15 00:00:00

  • Binding and hemagglutinating properties of the B subunit(s) of heat-labile enterotoxin isolated from human enterotoxigenic Escherichia coli.

    abstract::The binding and hemagglutinating activities of the B subunit(s) of the heat-labile enterotoxin (LTh-B) isolated from human enterotoxigenic Escherichia coli were investigated. The binding of 125I-labeled LTh-B to neuraminidase-treated human type B erythrocytes was most effectively inhibited by ganglioside GM1. A number...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90256-p

    authors: Sugii S,Tsuji T

    更新日期:1990-01-01 00:00:00

  • Colony immunoblot assay for the detection of hemolysin BL enterotoxin producing Bacillus cereus.

    abstract::Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.07.024

    authors: Moravek M,Wegscheider M,Schulz A,Dietrich R,Bürk C,Märtlbauer E

    更新日期:2004-09-01 00:00:00

  • Cell surface characteristics of nontypeable isolates of Streptococcus suis.

    abstract::Streptococcus suis is a worldwide cause of various swine infections and is also an important agent of zoonosis. Strains of S. suis are classified according to their serotype, and currently, 35 serotypes are recognized. The aim of this study was to characterize nontypeable isolates of S. suis with regard to their cell ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02086.x

    authors: Bonifait L,Gottschalk M,Grenier D

    更新日期:2010-10-01 00:00:00

  • A novel prophage lysin Ply5218 with extended lytic activity and stability against Streptococcus suis infection.

    abstract::Streptococcus suis (S. suis) is an emerging zoonotic agent that exhibits high level resistance to classic antibiotics and a heavy burden in the swine industry. Therefore alternative antibacterial agents need to be developed. A novel endolysin derived from the S. suis temperate phage phi5218, termed Ply5218, was identi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw186

    authors: Zhang H,Zhang C,Wang H,Yan YX,Sun J

    更新日期:2016-09-01 00:00:00

  • Growth, incidence and activities of dissimilatory sulfate-reducing bacteria in the human oral cavity.

    abstract::Viable counts and activities of sulfate-reducing bacteria were determined in the oral cavities of 12 healthy volunteers. Of these, 10 harboured viable sulfate-reducing bacteria populations. Six separate sites were sampled: the posterior tongue, anterior tongue, mid buccal mucosa, vestibular mucosa, supragingival plaqu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07591.x

    authors: Willis CL,Gibson GR,Allison C,Macfarlane S,Holt JS

    更新日期:1995-06-15 00:00:00

  • Neuraminidase (sialidase) activity of Haemophilus parasuis.

    abstract::Neuraminidase (sialidase), a potential virulence factor in bacteria, was demonstrated in Haemophilus parasuis, an invasive swine pathogen, but not in four other pathogens of the Pasteurellaceae family: H. influenzae, H. somnus, H. paragallinarum, or Actinobacillus pleuropneumoniae. H. parasuis neuraminidase had an aci...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb10438.x

    authors: Lichtensteiger CA,Vimr ER

    更新日期:1997-07-15 00:00:00

  • Laboratory-acquired lethal infections by potential bioweapons pathogens including Ebola in 2014.

    abstract::Researchers studying both cellular and viral disease agents in the laboratory have become infected since the early days of microbiology 150 years ago. However, in the early 21st century, new concerns about bioweapons being used to generate terror and also with a series of newly emerging or newly understood disease-cau...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnu008

    authors: Silver S

    更新日期:2015-01-01 00:00:00

  • Aerotactic responses in bacteria to photoreleased oxygen.

    abstract::Bacterial aerotaxis is a rapid response towards or away from oxygen. Here we report on the use of computer-assisted motion analysis coupled to flash photolysis of caged oxygen to quantify aerotactic responses in bacteria. The caged compound (mu-peroxo)(mu-hydroxo)bis[bis(bipyridyl) cobalt(III)] perchlorate liberates m...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11481.x

    authors: Yu HS,Saw JH,Hou S,Larsen RW,Watts KJ,Johnson MS,Zimmer MA,Ordal GW,Taylor BL,Alam M

    更新日期:2002-12-17 00:00:00

  • Induction of baroresistance by hydrogen peroxide, ethanol and cold-shock in Saccharomyces cerevisiae.

    abstract::The acquisition of tolerance to high hydrostatic pressure of 220 MPa (HHP) in response to a 0.4 mM hydrogen peroxide, 6% ethanol and cold-shock (10 degrees C) pretreatment for different lengths of times was studied in the yeast Saccharomyces cerevisiae. The protection conferred by these different treatments was simila...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.02.002

    authors: Palhano FL,Orlando MT,Fernandes PM

    更新日期:2004-04-01 00:00:00