Genetic investigation within Lactococcus garvieae revealed two genomic lineages.

Abstract:

:The diversity of a collection of 49 Lactococcus garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin, was explored using a molecular polyphasic approach comprising PCR-ribotyping, REP and RAPD-PCR analyses and a multilocus restriction typing (MLRT) carried out on six partial genes (atpA, tuf, dltA, als, gapC, and galP). This approach allowed high-resolution cluster analysis in which two major groups were distinguishable: one group included dairy isolates, the other group meat isolates. Unexpectedly, of the 12 strains coming from fish, four grouped with dairy isolates, whereas the others with meat isolates. Likewise, strains isolated from vegetables allocated between the two main groups. These findings revealed high variability within the species at both gene and genome levels. The observed genetic heterogeneity among L. garvieae strains was not entirely coherent with the ecological niche of origin of the strains, but rather supports the idea of an early separation of L. garvieae population into two independent genomic lineages.

journal_name

FEMS Microbiol Lett

authors

Ferrario C,Ricci G,Borgo F,Rollando A,Fortina MG

doi

10.1111/j.1574-6968.2012.02591.x

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

153-61

issue

2

eissn

0378-1097

issn

1574-6968

journal_volume

332

pub_type

信件
  • Supersusceptibility to hydrophobic antimicrobial agents and cell surface hydrophobicity in Branhamella catarrhalis.

    abstract::To clarify the cause of the supersusceptibility of Branhamella catarrhalis to macrolide antibiotics, which are well-known to be inactive to most Gram-negative bacteria, we determined its cell surface hydrophobicity by the partition experiment between water and hydrocarbons. Its cell surface was found to be markedly mo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(89)90488-6

    authors: Gotoh N,Tanaka S,Nishino T

    更新日期:1989-05-01 00:00:00

  • Structure and function of a cold shock domain fold protein, CspD, in Janthinobacterium sp. Ant5-2 from East Antarctica.

    abstract::A cold shock domain (CSD)-containing protein, CspD, of molecular mass ~7.28 kDa in a psychrotolerant Antarctic Janthinobacterium sp. Ant5-2 (ATCC BAA-2154) exhibited constitutive expression at 37, 22, 15, 4 and -1°C. The cspD gene encoding the CspD protein of Ant5-2 was cloned, sequenced and analyzed. The deduced prot...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02269.x

    authors: Mojib N,Andersen DT,Bej AK

    更新日期:2011-06-01 00:00:00

  • Isolation of the fenoxaprop-ethyl (FE)-degrading bacterium Rhodococcus sp. T1, and cloning of FE hydrolase gene feh.

    abstract::An enrichment culture which completely degraded fenoxaprop-ethyl (FE) was acquired by using FE as sole carbon source. An efficient FE-degrading strain T1 was isolated from the enrichment culture and identified as Rhodococcus sp. Strain T1 could degrade 94% of 100 mg L(-1) FE within 24 h and the metabolite fenoxaprop a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02376.x

    authors: Hou Y,Tao J,Shen W,Liu J,Li J,Li Y,Cao H,Cui Z

    更新日期:2011-10-01 00:00:00

  • Solubilization of zinc salts by a bacterium isolated from the air environment of a tannery.

    abstract::Airborne bacteria isolated from a tannery air environment were screened for the property of solubilization of insoluble zinc oxide and zinc phosphate. Out of 10 strains tested, a strain of Pseudomonas aeruginosa (CMG 823) showed the best solubilization and solubilized both zinc oxide and zinc phosphate. Colonies of th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11277.x

    authors: Fasim F,Ahmed N,Parsons R,Gadd GM

    更新日期:2002-07-16 00:00:00

  • Assessing the diversity of the g23 gene of T4-like bacteriophages from Lake Baikal with high-throughput sequencing.

    abstract::Based on second generation sequencing (MiSeq platform, Illumina), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obt...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx264

    authors: Potapov S,Belykh O,Krasnopeev A,Gladkikh A,Kabilov M,Tupikin A,Butina T

    更新日期:2018-02-01 00:00:00

  • Regulation of biosynthesis and transport of aromatic amino acids in low-GC Gram-positive bacteria.

    abstract::Computational comparative techniques were applied to analysis of the aromatic amino acid regulon in Gram-positive bacteria. A new candidate transcription regulation signal of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and shikimate kinase genes was identified in Streptococcus and Lactococcus species. New T-b...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00303-3

    authors: Panina EM,Vitreschak AG,Mironov AA,Gelfand MS

    更新日期:2003-05-28 00:00:00

  • Comparison of the effect of salinity on the D/H ratio of fatty acids of heterotrophic and photoautotrophic microorganisms.

    abstract::The core metabolism of microorganisms has a major influence on the hydrogen isotopic composition of their fatty acids. Heterotrophic microorganisms produce fatty acids with a deuterium to hydrogen (D/H) ratio either slightly depleted or enriched in D compared to the growth water, while photo- and chemoautotrophic micr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv065

    authors: Heinzelmann SM,Chivall D,M'Boule D,Sinke-Schoen D,Villanueva L,Damsté JS,Schouten S,van der Meer MT

    更新日期:2015-05-01 00:00:00

  • Aberrant gene for E1 Tor hemolysin from Vibrio cholerae non-O1, N037.

    abstract::Vibrio cholerae non-O1 strain N037 produced a hemolysin (NO37-Hly) which was antigenically similar to E1 Tor hemolysin (E1 Tor-Hly) but different in molecular size, hemolytic activity, and glucose binding capacity. In the gene encoding NO37-Hly, a 4-bp insertion into the structural gene for E1 Tor-Hly (hlyA) was found...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07876.x

    authors: Honma Y,Yamamoto K,Iwanaga M

    更新日期:1995-11-01 00:00:00

  • Growth, incidence and activities of dissimilatory sulfate-reducing bacteria in the human oral cavity.

    abstract::Viable counts and activities of sulfate-reducing bacteria were determined in the oral cavities of 12 healthy volunteers. Of these, 10 harboured viable sulfate-reducing bacteria populations. Six separate sites were sampled: the posterior tongue, anterior tongue, mid buccal mucosa, vestibular mucosa, supragingival plaqu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07591.x

    authors: Willis CL,Gibson GR,Allison C,Macfarlane S,Holt JS

    更新日期:1995-06-15 00:00:00

  • Triclosan: a widely used biocide and its link to antibiotics.

    abstract::Triclosan is the active ingredient in a multitude of health care and consumer products with germicidal properties, which have flooded the market in recent years in response to the public's fear of communicable bacteria. Although originally thought to kill bacteria by attacking multiple cellular targets, triclosan was ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2001.tb10772.x

    authors: Schweizer HP

    更新日期:2001-08-07 00:00:00

  • Molecular cloning of a novel allele of SMR1 which determines sulfometuron methyl resistance in Saccharomyces cerevisiae.

    abstract::A different mutation (SMR1B) to SMR1-410 (ilv2), which determines resistance to sulfometuron methyl in Saccharomyces cerevisiae, was cloned by PCR. Sequence analysis indicated a C to T change at position 575 of the ILV2 coding sequence, which results in a proline transition to leucine at position 192. Similarly to SMR...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08100.x

    authors: Xie Q,Jiménez A

    更新日期:1996-04-01 00:00:00

  • The neuA/flmD gene cluster of Helicobacter pylori is involved in flagellar biosynthesis and flagellin glycosylation.

    abstract::Helicobacter pylori possesses a gene (HP0326/JHP309) homologous to neuA of other bacteria, encoding a cytidyl monophosphate-N-acetylneuraminic acid synthetase-homologous enzyme in its N-terminal portion. We analysed the function of this gene, which is controlled by a flagellar class 2 sigma(54) promoter, in flagellar ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11176.x

    authors: Josenhans C,Vossebein L,Friedrich S,Suerbaum S

    更新日期:2002-05-07 00:00:00

  • Persister cells and tolerance to antimicrobials.

    abstract::Bacterial populations produce persister cells that neither grow nor die in the presence of microbicidal antibiotics. Persisters are largely responsible for high levels of biofilm tolerance to antimicrobials, but virtually nothing was known about their biology. Tolerance of Escherichia coli to ampicillin and ofloxacin ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00856-5

    authors: Keren I,Kaldalu N,Spoering A,Wang Y,Lewis K

    更新日期:2004-01-15 00:00:00

  • A novel phenol hydroxylase and catechol 2,3-dioxygenase from the thermophilic Bacillus thermoleovorans strain A2: nucleotide sequence and analysis of the genes.

    abstract::The new thermophilic Bacillus thermoleovorans strain A2 degrades phenol and cresols via the meta cleavage pathway. The first two enzymes involved in this process, the phenol hydroxylase and catechol 2,3-dioxygenase, encoded by the pheA and pheB genes respectively, were cloned and sequenced. The deduced amino acid sequ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12926.x

    authors: Duffner FM,Müller R

    更新日期:1998-04-01 00:00:00

  • Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome.

    abstract::Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary chicken kidney) cel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10586.x

    authors: Salvadori MR,Yamada AT,Yano T

    更新日期:2001-04-01 00:00:00

  • Isolation and characterization of a cytotoxin produced by Plesiomonas shigelloides P-1 strain.

    abstract::In order to clarify the enteropathogenicity of Plesiomonas shigelloides, we investigated a cytotoxin produced by the P-1 strain isolated from patients suffering from diarrhea. The cytotoxicity of the culture filtrate of the strain reached a maximum in culture at 37 degrees C after 12 h shaken in BHI medium. The cytoto...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.08.026

    authors: Okawa Y,Ohtomo Y,Tsugawa H,Matsuda Y,Kobayashi H,Tsukamoto T

    更新日期:2004-10-01 00:00:00

  • Direct profiling of the yeast dynamics in wine fermentations.

    abstract::We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09210.x

    authors: Cocolin L,Bisson LF,Mills DA

    更新日期:2000-08-01 00:00:00

  • Neisseria meningitidis transferrin-binding protein 1 expressed in Escherichia coli is surface exposed and binds human transferrin.

    abstract::A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambda Zap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06310.x

    authors: Palmer HM,Powell NB,Ala'Aldeen DA,Wilton J,Borriello SP

    更新日期:1993-06-15 00:00:00

  • bkaR is a TetR-type repressor that controls an operon associated with branched-chain keto-acid metabolism in Mycobacteria.

    abstract::This study describes how bkaR, a highly conserved mycobacterial TetR-like transcriptional repressor, regulates a number of nearby genes that have associations with branched-chain keto-acid metabolism. bkaR (MSMEG_4718) was deleted from the nonpathogenic species Mycobacterium smegmatis, and changes in global gene expre...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12196

    authors: Balhana RJ,Swanston SN,Coade S,Withers M,Sikder MH,Stoker NG,Kendall SL

    更新日期:2013-08-01 00:00:00

  • Identification and characterization of GroEL and DnaK homologues in Thiobacillus ferrooxidans.

    abstract::The major heat shock proteins from Thiobacillus ferrooxidans were identified as DnaK and GroEL equivalents by Western blotting and analysis of the N-terminal amino acid sequence of spots isolated from dried 2-D polyacrylamide electrophoresis gels. The T. ferrooxidans chaperonins showed 70% and 80% identity with the Es...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90147-g

    authors: Varela P,Jerez CA

    更新日期:1992-11-01 00:00:00

  • The plant pathogen Erwinia amylovora produces acyl-homoserine lactone signal molecules in vitro and in planta.

    abstract::We report for the first time the production of acyl homoserine lactones (AHLs) by Erwina amylovora, an important quarantine bacterial pathogen that causes fire blight in plants. E. amylovora produces one N-acyl homoserine lactone [a N-(3-oxo-hexanoyl)-homoserine lactone or a N-(3-hydroxy-hexanoyl)-homoserine lactone] ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.10.015

    authors: Venturi V,Venuti C,Devescovi G,Lucchese C,Friscina A,Degrassi G,Aguilar C,Mazzucchi U

    更新日期:2004-12-15 00:00:00

  • The ability of Vibrio vulnificus to use a synthetic hydrophilic heme compound, Fe-TPPS, as a single iron source.

    abstract::Vibrio vulnificus, an opportunistic human pathogen, can obtain iron from a variety of heme proteins. This process involves the digestion of heme proteins by an exoprotease to liberate protoheme (iron-protoporphyrin IX). In the present study, we tested whether this pathogen also uses a synthetic heme compound, Fe-alpha...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13452.x

    authors: Miyoshi S,Kamei T,Inami Y,Ota Y,Yamamoto S,Tomochika K,Shinoda S

    更新日期:1999-03-01 00:00:00

  • Identification of novel genes to assign enhanced tolerance to osmotic stress in Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae strains tolerant to osmotic stress are important for ethanol production during very high gravity (VHG) fermentation. We aimed to identify novel genes that confer enhanced tolerance to osmotic stress in S. cerevisiae. Two strains tolerant to up to 30% (w/v) glucose were isolated by screening a ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny149

    authors: Kim B,Kim HS

    更新日期:2018-07-01 00:00:00

  • A fungus-type beta-galactofuranan in the cultivated Trebouxia photobiont of the lichen Ramalina gracilis.

    abstract::A structural characterization of polysaccharides extracted from the aposymbiotically cultured photobiont of the lichen Ramalina gracilis was carried out in order to compare them with those previously found in the symbiotic thallus. The photobiont was isolated from thallus fragments, following the method of Yamamoto, a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.01.040

    authors: Cordeiro LM,Carbonero ER,Sassaki GL,Reis RA,Stocker-Wörgötter E,Gorin PA,Iacomini M

    更新日期:2005-03-01 00:00:00

  • Yeast genes involved in growth inhibition by Pseudomonas syringae pv. syringae syringomycin family lipodepsipeptides.

    abstract::Saccharomyces cerevisiae genes encoding functions necessary for inhibition by the Pseudomonas syringae pv. syringae cyclic lipodepsipeptide, syringomycin-E, were identified by mutant analyses. Syringomycin-E-resistant mutants were isolated, shown to contain single recessive mutations, and divided into eight gene compl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06595.x

    authors: Takemoto JY,Yu Y,Stock SD,Miyakawa T

    更新日期:1993-12-15 00:00:00

  • Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis.

    abstract::The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 2...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.10.004

    authors: Moran AP,Shiberu B,Ferris JA,Knirel YA,Senchenkova SN,Perepelov AV,Jansson PE,Goldberg JB

    更新日期:2004-12-01 00:00:00

  • Polyhydroxyalkanoate biosynthesis in Pseudomonas pseudoalcaligenes YS1.

    abstract::Pseudomonas pseudoalcaligenes strain YS1 isolated from oil contaminated soil was able to produce polyhydroxybutyrate blended with medium-chain-length polyhydroxyalkanoates (mcl PHA). PHA synthesis genes were cloned from this strain. A fadB (gene for fatty acid degradation) deleted mutant Escherichia coli KM32B (FADB::...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11247.x

    authors: Hang X,Lin Z,Chen J,Wang G,Hong K,Chen GQ

    更新日期:2002-06-18 00:00:00

  • Characterization of the desulforubidin operons from Desulfobacter vibrioformis and Desulfobulbus rhabdoformis.

    abstract::The genes encoding the desulforubidin type of dissimilatory sulfite reductase (Dsr) from the sulfate-reducing bacteria Desulfobacter vibrioformis and Desulfobulbus rhabdoformis were cloned and sequenced. Similar to the genes for dissimilatory sulfite reductase from the genera Archaeoglobus, Desulfovibrio and Desulfoto...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09079.x

    authors: Larsen,Lien T,Birkeland NK

    更新日期:2000-05-01 00:00:00

  • Taxonomic revision of the cellulose-degrading fungus Acremonium cellulolyticus nomen nudum to Talaromyces based on phylogenetic analysis.

    abstract::The cellulase-producing fungal strain Y-94, isolated in Japan and invalidly described as Acremonium cellulolyticus nom. nud. strain Y-94, seldom forms enteroarthric conidia under nutrient starvation conditions. Phylogenetic analysis using ITS1-5.8S-ITS2 and RNA polymerase II large subunit gene sequences revealed that ...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12352

    authors: Fujii T,Hoshino T,Inoue H,Yano S

    更新日期:2014-02-01 00:00:00

  • In vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea.

    abstract::The genus Xanthomonas is composed of several species that cause severe crop losses around the world. In Latin America, one of the most relevant species is Xanthomonas campestris pv. campestris, which is responsible for black rot in cruciferous plants. This pathogen causes yield losses in several cultures, including ca...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01090.x

    authors: Andrade AE,Silva LP,Pereira JL,Noronha EF,Reis FB Jr,Bloch C Jr,dos Santos MF,Domont GB,Franco OL,Mehta A

    更新日期:2008-04-01 00:00:00