Genetic investigation within Lactococcus garvieae revealed two genomic lineages.


:The diversity of a collection of 49 Lactococcus garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin, was explored using a molecular polyphasic approach comprising PCR-ribotyping, REP and RAPD-PCR analyses and a multilocus restriction typing (MLRT) carried out on six partial genes (atpA, tuf, dltA, als, gapC, and galP). This approach allowed high-resolution cluster analysis in which two major groups were distinguishable: one group included dairy isolates, the other group meat isolates. Unexpectedly, of the 12 strains coming from fish, four grouped with dairy isolates, whereas the others with meat isolates. Likewise, strains isolated from vegetables allocated between the two main groups. These findings revealed high variability within the species at both gene and genome levels. The observed genetic heterogeneity among L. garvieae strains was not entirely coherent with the ecological niche of origin of the strains, but rather supports the idea of an early separation of L. garvieae population into two independent genomic lineages.


FEMS Microbiol Lett


Ferrario C,Ricci G,Borgo F,Rollando A,Fortina MG




Has Abstract


2012-07-01 00:00:00












  • Involvement of O8-antigen in altering beta-lactam antibiotic susceptibilities in Escherichia coli.

    abstract::In spite of being dispensable, O-antigens are believed to facilitate various cellular processes and alter antibiotic sensitivities. Escherichia coli K-12 (CS109) strains are lacking in O-antigens and are reported to be sensitive to antibiotics. To our surprise, E. coli 2443 (expressing O8-antigen) manifested two- to f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sarkar SK,Ghosh AS

    更新日期:2008-05-01 00:00:00

  • Purification and characterization of a membrane-associated ATPase from Natronococcus occultus, a haloalkaliphilic archaeon.

    abstract::Isolated membranes of the extreme haloalkaliphilic archaeon Natronococcus occultus were able to hydrolyze ATP via an ATPase, which required the presence of Mg(2+), high concentrations of NaCl, and a pH value of 9. The native molecular mass of the purified ATPase was 130 kDa and was composed of 74- and 61-kDa subunits....

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Eddy ML,Jablonski PE

    更新日期:2000-08-15 00:00:00

  • STa-induced translocation of protein kinase C from cytosol to membrane in rat enterocytes.

    abstract::Escherichia coli heat stable enterotoxin (STa) binds to isolated rat intestinal epithelial cells and triggers a cascade reaction including increase of intracellular calcium levels ([Ca(2+)](i)) and membrane bound protein kinase C (PKC) activity. In response to STa, the cytosolic PKC activity falls from 110 to 35 nmol ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ganguly U,Chaudhury AG,Basu A,Sen PC

    更新日期:2001-10-16 00:00:00

  • Lipid modification of prelipoproteins is dispensable for growth in vitro but essential for virulence in Streptococcus pneumoniae.

    abstract::A Deltalgt (Lgt, lipoprotein diacylglyceryl transferase) isogenic mutant was obtained which indicates that lgt is not essential for cell growth in vitro, like in the Gram-positive bacterium Bacillus subtilis, but unlike in the proteobacteria Escherichia coli and Salmonella typhimurium. The mutation was transduced to a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Petit CM,Brown JR,Ingraham K,Bryant AP,Holmes DJ

    更新日期:2001-06-25 00:00:00

  • Mechanisms of action of ostrich beta-defensins against Escherichia coli.

    abstract::To understand their mechanism of antimicrobial activity against Gram-negative bacteria, ostrich beta-defensins, ostricacins-1 and 2 (Osp-1 and Osp-2), were compared with those of sheep myeloid antimicrobial peptide (SMAP)-29 and human neutrophil peptide (HNP)-1, well-characterized sheep alpha-helical and human alpha-d...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sugiarto H,Yu PL

    更新日期:2007-05-01 00:00:00

  • Roles of siderophore in manganese-oxide reduction by Shewanella oneidensis MR-1.

    abstract::Dissimilatory metal-reducing bacteria (DMRB), such as Shewanella oneidensis MR-1, are of great interest for their importance in the biogeochemical cycling of metals and utility in biotechnological processes, such as bioremediation and microbial fuel cells. To identify genes necessary for metal reduction, this study co...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kouzuma A,Hashimoto K,Watanabe K

    更新日期:2012-01-01 00:00:00

  • Description of Sulfitobacter sediminilitoris sp. nov., isolated from a tidal flat.

    abstract::A Gram-stain-negative bacterial strain, JBTF-M27T, was isolated from a tidal flat from Yellow Sea, Republic of Korea. Neighbor-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M27T fell within the clade comprising the type strains of Sulfitobacter species. Strain JBTF-M27T exhibited the hig...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Park S,Chen S,Lee JS,Kim W,Yoon JH

    更新日期:2020-05-01 00:00:00

  • Rapid accumulation of intracellular 2-keto-3-deoxy-6-phosphogluconate in an Entner-Doudoroff aldolase mutant results in bacteriostasis.

    abstract::The accumulation of 2-keto-3-deoxy-6-phosphogluconate, the key intermediate of the Entner-Doudoroff pathway, has long been thought to inhibit growth of bacteria, but careful measurements of 2-keto-3-deoxy-6-phosphogluconate accumulation by growing cells and the correlation of intracellular 2-keto-3-deoxy-6-phosphogluc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fuhrman LK,Wanken A,Nickerson KW,Conway T

    更新日期:1998-02-15 00:00:00

  • A surface polysaccharide forms when gonococci are converted to serum resistance by cytidine 5'-monophospho-N-acetyl neuraminic acid.

    abstract::A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fox AJ,Curry A,Rowland PL,Lancaster S,Jones DM,Parsons NJ,Cole JA,Smith H

    更新日期:1990-01-01 00:00:00

  • Repression of the TreR transcriptional regulator in Streptococcus mutans by the global regulator, CcpA.

    abstract::Streptococcus mutans, the etiologic agent of dental caries in humans, is considered a dominating force in the oral microbiome due to its highly-evolved propensity for survival. The oral pathogen encodes an elaborate array of regulatory elements, including the carbon catabolite-responsive regulator, CcpA, a global regu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lindsay EL,Faustoferri RC,Quivey RG

    更新日期:2021-01-16 00:00:00

  • Analysis of the role of the Aspergillus niger aminolevulinic acid synthase (hemA) gene illustrates the difference between regulation of yeast and fungal haem- and sirohaem-dependent pathways.

    abstract::To increase knowledge on haem biosynthesis in filamentous fungi like Aspergillus niger, pathway-specific gene expression in response to haem and haem intermediates was analysed. This analysis showed that iron, 5'-aminolevulinic acid (ALA) and possibly haem control haem biosynthesis mostly via modulating expression of ...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Franken AC,Lokman BC,Ram AF,van den Hondel CA,de Weert S,Punt PJ

    更新日期:2012-10-01 00:00:00

  • An ABC transporter involved in the control of streptomycin production in Streptomyces griseus.

    abstract::We screened for a gene that inhibits streptomycin production in Streptomyces griseus when it is introduced on a high-copy-number plasmid pIJ702, and obtained a plasmid pKM545. The introduction of pKM545 abolished streptomycin production on all media tested including YMP-sugar and Nutrient broth. S1 protection analysis...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Takano H,Toriumi N,Hirata M,Amano T,Ohya T,Shimada R,Kusada H,Amano S,Matsuda K,Beppu T,Ueda K

    更新日期:2016-07-01 00:00:00

  • Infection of human B lymphoma cells by Mycoplasma fermentans induces interaction of its elongation factor with the intracytoplasmic domain of Epstein-Barr virus receptor (gp140, EBV/C3dR, CR2, CD21).

    abstract::Human cell lines are often infected by mycoplama strains. We have demonstrated that when infected by Mycoplasma fermentans, human B lymphoma cell proliferation increased strongly. These infected B cells expressed a p45 kDa protein which interacted with the intracellular domain of CD21, the EBV/C3d receptor. p45 analys...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Balbo M,Barel M,Lottin-Divoux S,Jean D,Frade R

    更新日期:2005-08-15 00:00:00

  • Transcriptional analysis of the sigA and sigB genes of Brevibacterium lactofermentum.

    abstract::Transcription of the sigA and sigB genes of Brevibacterium lactofermentum, encoding the principal sigma factors of RNA polymerase, has been investigated by Northern blot and primer extension analysis. Northern hybridizations revealed that sigA is transcribed as a monocistronic mRNA of 1.7 kb and sigB gives two transcr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Oguiza JA,Marcos AT,Martín JF

    更新日期:1997-08-01 00:00:00

  • Incorporation of choline into Streptococcus pneumoniae cell wall antigens: evidence for choline kinase activity.

    abstract::The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Whiting GC,Gillespie SH

    更新日期:1996-05-01 00:00:00

  • Dr fimbriae coding region associated hemolytic activity of Escherichia coli.

    abstract::We investigated the hemolytic activity of Escherichia coli strain EC901 carrying plasmid pBJN406 containing genes draA-E involved in expression of the mannose-resistant Dr hemagglutinin, and in its isogenic insertion mutants devised with Tn5, Tn3, and TnphoA. While E. coli BN406 displayed rapid hemolytic activity agai...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Goluszko P,Nowicki S,Kaul AK,Pham T,Nowicki BJ

    更新日期:1995-07-15 00:00:00

  • Construction of Escherichia coli-Arthrobacter-Rhodococcus shuttle vectors based on a cryptic plasmid from Arthrobacter rhombi and investigation of their application for functional screening.

    abstract::A cryptic plasmid from Arthrobacter rhombi PRH1, designated as pPRH, was sequenced and characterized. It was 5000 bp in length with a G+C content of 66 mol%. The plasmid pPRH was predicted to encode six putative open reading frames (ORFs), in which ORF2 and ORF3 formed the minimal replicon of plasmid pPRH and shared 5...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Stanislauskiene R,Gasparaviciute R,Vaitekunas J,Meskiene R,Rutkiene R,Casaite V,Meskys R

    更新日期:2012-02-01 00:00:00

  • A novel colorimetric method for the detection of Escherichia coli using cytochrome c peroxidase-encoding bacteriophage.

    abstract::A new rapid and simple method was developed for the detection of Escherichia coli by constructing a recombinant T4 phage carrying the cytochrome c peroxidase gene derived from Saccharomyces cerevisiae (T4ccp) using which, the colorimetric detection of E. coli K12 was examined. The oxidation activity toward the chromog...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Hoang HA,Abe M,Nakasaki K

    更新日期:2014-03-01 00:00:00

  • Induced expression of the Serratia entomophila Sep proteins shows activity towards the larvae of the New Zealand grass grub Costelytra zealandica.

    abstract::Serratia entomophila and Serratia proteamaculans cause amber disease of the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). Three genes required for virulence, sepABC, are located on a large plasmid, pADAP. The translated products of the sep genes are members of the toxin complex (Tc) family of insecticid...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hurst MR,Jones SM,Tan B,Jackson TA

    更新日期:2007-10-01 00:00:00

  • Inactivation of the fis gene leads to reduced growth rate.

    abstract::The FIS protein (factor for inversion stimulation) is known to activate the transcription of rRNA and tRNA operons in Escherichia coli. In this report the growth behaviour of a fis bacterium was studied under steady-state conditions and in competition experiments with a wild-type bacterium in a chemostat. The growth r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nilsson L,Verbeek H,Hoffmann U,Haupt M,Bosch L

    更新日期:1992-11-15 00:00:00

  • A unique and common restriction fragment pattern of the nucleotide sequences homologous to the genome of vf33, a filamentous bacteriophage, in pandemic strains of Vibrio parahaemolyticus O3:K6 O4:K68, and O1:K untypeable.

    abstract::Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus. We performed Southern blot hybridization analysis to examine the distribution of Vf33-related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chang B,Yoshida S,Miyamoto H,Ogawa M,Horikawa K,Ogata K,Nishibuchi M,Taniguchi H

    更新日期:2000-11-15 00:00:00

  • Phylogenetic relationships between Tuber pseudoexcavatum, a Chinese truffle, and other Tuber species based on parsimony and distance analysis of four different gene sequences.

    abstract::Phylogenetic relationships between Tuber pseudoexcavatum and other Tuber species were investigated by studying the sequences of four genes: 5.8S-ITS2, beta-tubulin, protein kinase C and elongation factor 1alpha. The four phylogenetic trees allowed to differentiate the black truffle clade, composed of two subclades, on...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wang Y,Tan ZM,Zhang DC,Murat C,Jeandroz S,Le Tacon F

    更新日期:2006-06-01 00:00:00

  • Microorganisms form exocellular structures, trophosomes, to facilitate biodegradation of oil in aqueous media.

    abstract::Cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. Among the microorganisms studied here, the yeasts (Schwanniomyces occidentalis, Torulopsis candida, Candida tro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dmitriev VV,Crowley D,Rogachevsky VV,Negri CM,Rusakova TG,Kolesnikova SA,Akhmetov LI

    更新日期:2011-02-01 00:00:00

  • Respiration of [14C]alanine by the ectomycorrhizal fungus Paxillus involutus.

    abstract::The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [14C]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [14C]alanine was further actively metabolised to g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chalot M,Brun A,Finlay RD,Söderström B

    更新日期:1994-08-01 00:00:00

  • Cell wall constituents of Microcystis sp. PCC 7806.

    abstract::Cell walls of Microcystis sp. PCC 7806 were purified from cell homogenates by sucrose density centrifugation and Triton X-100 extraction. The outer membrane contained carotenoids, two major peptidoglycan-associated proteins (Mr 49,000 and 52,000), and lipopolysaccharide (LPS) as indicated by the presence of 3-hydroxy ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jürgens UJ,Martin C,Weckesser J

    更新日期:1989-11-01 00:00:00

  • Bacteria associated with arbuscular mycorrhizal fungi within roots of plants growing in a soil highly contaminated with aliphatic and aromatic petroleum hydrocarbons.

    abstract::Arbuscular mycorrhizal fungi (AMF) belong to phylum Glomeromycota, an early divergent fungal lineage forming symbiosis with plant roots. Many reports have documented that bacteria are intimately associated with AMF mycelia in the soil. However, the role of these bacteria remains unclear and their diversity within intr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Iffis B,St-Arnaud M,Hijri M

    更新日期:2014-09-01 00:00:00

  • Membrane proteins with molecular masses of 88, 90 and 150 kDa are responsible for binding of human immunoglobulin G Fc fragment to the native cells of Mycoplasma salivarium.

    abstract::Mycoplasma salivarium cells bound the Fc fragment of human immunoglobulin G. The activity was remarkably enhanced by Mn2+, but not by Mg2+ and Ca2+; significantly inhibited by D-mannose; and reduced by pronase treatment of the cells. About 90% of the cells treated with pronase were not disrupted. Sodium dodecyl sulfat...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shibata K,Sawa Y,Inoue S,Noda M,Watanabe T

    更新日期:1994-11-01 00:00:00

  • Cross-utilization and expression of outer membrane receptor proteins for siderophore uptake by Diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) gut bacteria.

    abstract::Siderophore production by entomo- and phytopathogens, plus the cross-utilization of these siderophores and expression of outer membrane receptor proteins (OMRPs) by Diamondback moth (DBM) gut bacterial strains, were all examined. All the tested strains grew in the presence of 2, 2'-dipyridyl, and the Brachybacterium s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Indiragandhi P,Anandham R,Madhaiyan M,Kim GH,Sa T

    更新日期:2008-12-01 00:00:00

  • Respective role of the 42- and 51-kDa components of the Bacillus sphaericus toxin overexpressed in Bacillus thuringiensis.

    abstract::The 42- and 51-kDa protein genes of Bacillus sphaericus 1593 have been subcloned independently downstream from the cytA gene promoter of Bacillus thuringiensis serovar israelensis and introduced into a non-mosquitocidal strain of Bacillus thuringiensis. Consequently, each protein was overproduced and accumulated as in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nicolas L,Nielsen-Leroux C,Charles JF,Delécluse A

    更新日期:1993-02-01 00:00:00

  • Construction and transduction of a shuttle vector bearing the cos site of Streptomyces phage phi C31 and determination of its cohesive ends.

    abstract::A plasmid has been constructed which is a bifunctional vector for Escherichia coli and for streptomycetes, containing the cos site of Streptomyces phage phi C31. It can be efficiently transduced into S. lividans 66 and into several other Streptomyces species. The nucleotide sequence of a 311-bp DNA fragment containing...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kobler L,Schwertfirm G,Schmieger H,Bolotin A,Sladkova I

    更新日期:1991-03-01 00:00:00