Interactions between Bacillus subtilis early spore coat morphogenetic proteins.

Abstract:

:When challenged by stresses such as starvation, the soil bacterium Bacillus subtilis produces an endospore surrounded by a proteinaceous coat composed of >70 proteins that are organized into three main layers: an amorphous undercoat, lightly staining lamellar inner coat and electron-dense outer coat. This coat protects the spore against a variety of chemicals or lysozyme. Mutual interactions of the coat's building blocks are responsible for the formation of this structurally complex and extraordinarily resistant shell. However, the assembly process of spore coat proteins is still poorly understood. In the present work, the main focus is on the three spore coat morphogenetic proteins: SpoIVA, SpoVID and SafA. Direct interaction between SpoIVA and SpoVID proteins was observed using a yeast two-hybrid assay and verified by coexpression experiment followed by Western blot analysis. Coexpression experiments also confirmed previous findings that SpoVID and SafA directly interact, and revealed a novel interaction between SpoIVA and SafA. Moreover, gel filtration analysis revealed that both SpoIVA and SpoVID proteins form large oligomers.

journal_name

FEMS Microbiol Lett

authors

Müllerová D,Krajčíková D,Barák I

doi

10.1111/j.1574-6968.2009.01737.x

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

74-85

issue

1

eissn

0378-1097

issn

1574-6968

pii

FML1737

journal_volume

299

pub_type

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