Activity of the Streptomyces coelicolor stress-response sigma factor sigmaH is regulated by an anti-sigma factor.


:The alternative sigma factor sigmaH has been shown to play an important role in stress response and morphological differentiation in Streptomyces coelicolor. Its gene, sigH, is located in an operon with the gene encoding proposed anti-sigma factor UshX, and one of the promoters directing expression of the operon is dependent upon sigH. To clarify the function of S. coelicolor UshX, both the sigmaH and UshX proteins were overproduced in Escherichia coli and purified. In an in vitro transcription assay, sigmaH, after complementation with S. coelicolor core RNA polymerase, was able to recognize the sigH-dependent promoter, sigH-P2. This transcription was inhibited by UshX, if it was incubated with sigmaH prior to the addition of the core RNA polymerase. When sigmaH and UshX were incubated and electrophoresed through non-denaturing polyacrylamide gels, they formed a specific complex. These results showed that UshX is a specific anti-sigma factor for sigmaH, and the S. coelicolor sigH operon is directly autoregulated.


FEMS Microbiol Lett


Sevcikova B,Kormanec J





Has Abstract


2002-04-09 00:00:00














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    abstract::Chemical and molecular taxonomic studies were performed on a representative strain of some lactic acid bacteria of unknown taxonomic position isolated from salmonid fish. The results demonstrate that the fish bacterium represents a new species of the genus Lactococcus for which the name Lactococcus piscium sp. nov. is...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Williams AM,Fryer JL,Collins MD

    更新日期:1990-03-01 00:00:00

  • NtrBC-dependent expression from the Rhizobium meliloti dctA promoter in Escherichia coli.

    abstract::Effects of the two-component sensor-regulator pairs DctBD and NtrBC upon the expression of a dctA::phoA fusion from Rhizobium meliloti were determined under excess and limiting nitrogen concentrations in Escherichia coli. Results indicated that NtrBC affected transcription from the dctA promoter on a number of regulat...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Allaway D,Boesten B,O'Gara F

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  • Surface Listeria monocytogenes carbohydrate-binding components revealed by agglutination with neoglycoproteins.

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    journal_title:FEMS microbiology letters

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    pub_type: 杂志文章


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  • Bacillus cereus and its food poisoning toxins.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Varela P,Jerez CA

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 评论,杂志文章


    authors: Gams W,Stalpers JA

    更新日期:1994-07-01 00:00:00