Lipid modification of prelipoproteins is dispensable for growth in vitro but essential for virulence in Streptococcus pneumoniae.

Abstract:

:A Deltalgt (Lgt, lipoprotein diacylglyceryl transferase) isogenic mutant was obtained which indicates that lgt is not essential for cell growth in vitro, like in the Gram-positive bacterium Bacillus subtilis, but unlike in the proteobacteria Escherichia coli and Salmonella typhimurium. The mutation was transduced to a virulent strain. A 5 log attenuation was observed in a respiratory tract model of infection. Metabolic labeling by [U-14C]palmitate revealed the presence of eight to ten lipoproteins in the wild-type strain only, with molecular masses between 15 and 80 kDa. Our findings suggest a major difference in the role of lipoproteins in Gram-positive bacteria versus the proteobacteria.

journal_name

FEMS Microbiol Lett

authors

Petit CM,Brown JR,Ingraham K,Bryant AP,Holmes DJ

doi

10.1111/j.1574-6968.2001.tb10720.x

keywords:

subject

Has Abstract

pub_date

2001-06-25 00:00:00

pages

229-33

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109701002336

journal_volume

200

pub_type

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