Lipid modification of prelipoproteins is dispensable for growth in vitro but essential for virulence in Streptococcus pneumoniae.


:A Deltalgt (Lgt, lipoprotein diacylglyceryl transferase) isogenic mutant was obtained which indicates that lgt is not essential for cell growth in vitro, like in the Gram-positive bacterium Bacillus subtilis, but unlike in the proteobacteria Escherichia coli and Salmonella typhimurium. The mutation was transduced to a virulent strain. A 5 log attenuation was observed in a respiratory tract model of infection. Metabolic labeling by [U-14C]palmitate revealed the presence of eight to ten lipoproteins in the wild-type strain only, with molecular masses between 15 and 80 kDa. Our findings suggest a major difference in the role of lipoproteins in Gram-positive bacteria versus the proteobacteria.


FEMS Microbiol Lett


Petit CM,Brown JR,Ingraham K,Bryant AP,Holmes DJ





Has Abstract


2001-06-25 00:00:00














  • Environmental pH affects transcriptional responses to cadmium toxicity in Escherichia coli K-12 (MG1655).

    abstract::It has been widely reported that pH mediates cadmium toxicity to bacteria. We used a tripartite approach to investigate mechanisms by which pH affects cadmium toxicity that included analyses of: (1) growth kinetics, (2) global gene expression, and (3) cadmium speciation. Cadmium extended the lag phase at pH 7, but not...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Worden CR,Kovac WK,Dorn LA,Sandrin TR

    更新日期:2009-04-01 00:00:00

  • Glucose-6-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima: expression of the g6pd gene and characterization of an extremely thermophilic enzyme.

    abstract::The gene (open reading frame Tm1155, g6pd) encoding glucose-6-phosphate dehydrogenase (G6PD, EC of the hyperthermophilic bacterium Thermotoga maritima was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparent molecular mass of 95 kDa composed o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hansen T,Schlichting B,Schönheit P

    更新日期:2002-11-05 00:00:00

  • Intraspecies polymorphisms of Kluyveromyces marxianus strains from Yaghnob valley.

    abstract::In this study, 29 strains of Kluyveromyces marxianus with peculiar genetic and phenotypic traits previously isolated from a fermented goat milk of Yaghnob valley were investigated for chromosome length polymorphism (CLP) by PFGE, adhesion properties and carbon usage by Biolog analysis. Obtained data showed that strain...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Perpetuini G,Tittarelli F,Mattarelli P,Modesto M,Cilli E,Suzzi G,Tofalo R

    更新日期:2018-03-01 00:00:00

  • The activity and stability of cell-associated activity of bovicin HC5, a bacteriocin from Streptococcus bovis HC5.

    abstract::Streptococcus bovis HC5 cultures released a broad spectrum lantibiotic, bovicin HC5, into the cell-fee culture supernatant after they reached stationary phase, but most of the antibacterial activity remained cell-associated. Cell-associated bovicin HC5 was more resistant to degradation by Pronase E than the cell-free ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Xavier BM,Houlihan AJ,Russell JB

    更新日期:2008-06-01 00:00:00

  • Widespread ability of fungi to drive quinone redox cycling for biodegradation.

    abstract::Wood-rotting fungi possess remarkably diverse extracellular oxidation mechanisms, including enzymes, such as laccase and peroxidases, and Fenton chemistry. The ability to biologically drive Fenton chemistry by the redox cycling of quinones has previously been reported to be present in both ecologically diverging main ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Krueger MC,Bergmann M,Schlosser D

    更新日期:2016-06-01 00:00:00

  • Aerobic microbial communities in the sediments of a marine oxygen minimum zone.

    abstract::The ecology of aerobic microorganisms is never explored in marine oxygen minimum zone (OMZ) sediments. Here we reveal aerobic bacterial communities along ∼3 m sediment-horizons of the eastern Arabian Sea OMZ. Sulfide-containing sediment-cores retrieved from 530 mbsl (meters beneath the sea-level) and 580 mbsl were exp...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bhattacharya S,Roy C,Mandal S,Sarkar J,Rameez MJ,Mondal N,Mapder T,Chatterjee S,Pyne P,Alam M,Haldar PK,Roy R,Fernandes S,Peketi A,Chakraborty R,Mazumdar A,Ghosh W

    更新日期:2020-10-16 00:00:00

  • Environmental adaptation factors of two yeasts isolated from the leachate of a uranium mineral heap.

    abstract::Two yeasts from the effluent of a uranium mineral heap were identified as Candida sorbophila and Rhodotorula mucilaginosa. They are well adapted to such an environment as shown by their tolerance to metals and acid pH. However, different mechanisms could be involved in the adaptation: C. sorbophila could be considered...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: de Silóniz MI,Payo EM,Callejo MA,Marquina D,Peinado JM

    更新日期:2002-05-07 00:00:00

  • Chemical and electroporated transformation of Edwardsiella ictaluri using three different plasmids.

    abstract::Transfer of DNA by conjugation has been the method generally used for genetic manipulation of Edwardsiella ictaluri because, previously, attempts to transform E. ictaluri by the uptake of naked DNA have apparently failed. We report here the successful transformation of seven strains of E. ictaluri using electroporatio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Russo R,Panangala VS,Wood RR,Klesius PH

    更新日期:2009-09-01 00:00:00

  • Characterization of the membrane sensor PenJ for beta-lactam antibiotics from Bacillus licheniformis by amino acid substitution.

    abstract::The PenJ protein of the penicillinase gene (penP) expression system from Bacillus licheniformis is an antirepressor and membrane receptor for beta-lactam antibiotics. A putative beta-lactam antibiotic binding site including Ser402 and Lys405, which are homologous to the conserved sequence for the beta-lactam binding s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Takagi M,Ohta T,Johki S,Imanaka T

    更新日期:1993-06-01 00:00:00

  • Spirochaeta smaragdinae sp. nov., a new mesophilic strictly anaerobic spirochete from an oil field.

    abstract::An obligately anaerobic spirochete designated strain SEBR 4228T (T = type strain) was isolated from an oil field of Congo, Central Africa. The strain grew optimally with a sodium chloride concentration of 5% (sodium chloride concentration) growth range 1.0-10%) at 37 degrees C (growth temperature range 20-40 degrees C...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Magot M,Fardeau ML,Arnauld O,Lanau C,Ollivier B,Thomas P,Patel BK

    更新日期:1997-10-15 00:00:00

  • Functional investigation of residue G791 of Escherichia coli 16S rRNA: implication of initiation factor 1 in the restoration of P-site function.

    abstract::Using a specialized ribosome system, previous studies have identified G791 in Escherichia coli 16S rRNA as an invariant and essential residue for ribosome function. To investigate the functional role of G791, we searched for multicopy suppressors that partially restored the protein synthesis ability of mutant ribosome...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Song WS,Ryou SM,Kim HM,Jeon CO,Kim JM,Han SH,Kim SW,Szatkiewicz JP,Cunningham PR,Lee K

    更新日期:2010-12-01 00:00:00

  • Characterization of antimicrobial resistance in Salmonella enterica food and animal isolates from Colombia: identification of a qnrB19-mediated quinolone resistance marker in two novel serovars.

    abstract::Ninety-three Salmonella isolates recovered from commercial foods and exotic animals in Colombia were studied. The serotypes, resistance profiles and where applicable the quinolone resistance genes were determined. Salmonella Anatum (n=14), Uganda (19), Braenderup (10) and Newport (10) were the most prevalent serovars,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Karczmarczyk M,Martins M,McCusker M,Mattar S,Amaral L,Leonard N,Aarestrup FM,Fanning S

    更新日期:2010-12-01 00:00:00

  • Direct profiling of the yeast dynamics in wine fermentations.

    abstract::We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cocolin L,Bisson LF,Mills DA

    更新日期:2000-08-01 00:00:00

  • Regulation of nitrogen fixation in Azospirillum brasilense Sp7: involvement of nifA, glnA and glnB gene products.

    abstract::The expression of nifA-, niH- and nifB-lacZ fusions was examined in different mutants of Azospirillum brasilense. Mutations in nifA, glnA and glnB severely impaired the expression of nifH- and nifB-lacZ fusions. By contrast, a nifA-lacZ fusion was not affected in a nifA or a glnB background and was only partially impa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Liang YY,de Zamaroczy M,Arsène F,Paquelin A,Elmerich C

    更新日期:1992-12-15 00:00:00

  • SEF17 fimbriae are essential for the convoluted colonial morphology of Salmonella enteritidis.

    abstract::Salmonella enteritidis isolated from poultry infections generated a convoluted colonial morphology after 48 h growth on colonisation factor antigen (CFA) agar at 25 degrees C. A mutant S. enteritidis defective for the elaboration of the SEF17 fimbrial antigen, in which the agf gene cluster was inactivated by insertion...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Allen-Vercoe E,Dibb-Fuller M,Thorns CJ,Woodward MJ

    更新日期:1997-08-01 00:00:00

  • Thiabendazole resistance and mutations in the beta-tubulin gene of Penicillium expansum strains isolated from apples and pears with blue mold decay.

    abstract::Penicillium expansum is the causal agent of blue mold rot, a postharvest decay of stored fruits. This fungus also produces the mycotoxins patulin and citrinin. Control of P. expansum still relies mainly on the use of fungicides such as thiabendazole. Since its introduction, resistant strains have been reported. The ai...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cabañas R,Castellá G,Abarca ML,Bragulat MR,Cabañes FJ

    更新日期:2009-08-01 00:00:00

  • Molecular characterization of newly identified IS3, IS4 and IS30 insertion sequence-like elements in Mycoplasma bovis and their possible roles in genome plasticity.

    abstract::Insertion sequences (ISs) are mobile genetic elements widely distributed among bacteria. Their impact on the bacterial genome is multifold, including transfer of genetic information, shuttle of adaptive traits and influence on the genomic content. As a result, ISs play an important role in the organization, plasticity...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Lysnyansky I,Calcutt MJ,Ben-Barak I,Ron Y,Levisohn S,Methé BA,Yogev D

    更新日期:2009-05-01 00:00:00

  • Denitrifying haloarchaea: sources and sinks of nitrogenous gases.

    abstract::Haloarchaea thrive under saline and hypersaline conditions and often dominate microbial communities in saltmarshes, salted lakes/soils and some oceanic areas. Some of the predominant species show denitrifying capabilities, although it remains unclear whether they are complete or partial denitrifiers. As complete denit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Torregrosa-Crespo J,Bergaust L,Pire C,Martínez-Espinosa RM

    更新日期:2018-02-01 00:00:00

  • Development of TaqMan assays for the quantitative detection of Fusarium avenaceum/Fusarium tricinctum and Fusarium poae esyn1 genotypes from cereal grain.

    abstract::Fungi of the genus Fusarium are important plant pathogens and contaminants of cereal grains producing different types of mycotoxins. Enniatins are a group of mycotoxins with ionophoric properties frequently detected in North European grains. Within the Fusarium complex responsible for grain infection, Fusarium avenace...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kulik T,Jestoi M,Okorski A

    更新日期:2011-01-01 00:00:00

  • Hydroxyurea inhibits intracellular Toxoplasma gondii multiplication.

    abstract::Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: de Melo EJ,Mayerhoffer RO,de Souza W

    更新日期:2000-04-01 00:00:00

  • Neuraminidase (sialidase) activity of Haemophilus parasuis.

    abstract::Neuraminidase (sialidase), a potential virulence factor in bacteria, was demonstrated in Haemophilus parasuis, an invasive swine pathogen, but not in four other pathogens of the Pasteurellaceae family: H. influenzae, H. somnus, H. paragallinarum, or Actinobacillus pleuropneumoniae. H. parasuis neuraminidase had an aci...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lichtensteiger CA,Vimr ER

    更新日期:1997-07-15 00:00:00

  • Mapping of nutrient-induced biochemical changes in living algal cells using synchrotron infrared microspectroscopy.

    abstract::High quality Fourier transform infrared (FTIR) spectra were acquired from living Micrasterias hardyi cells maintained in an IR transparent flow-through cell using a FTIR microscope coupled to a synchrotron light source. Spectral maps of living, nutrient-replete cells showed band intensities consistent with the known l...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Heraud P,Wood BR,Tobin MJ,Beardall J,McNaughton D

    更新日期:2005-08-15 00:00:00

  • The Aspergillus niger acuA and acuB genes correspond to the facA and facB genes in Aspergillus nidulans.

    abstract::Mutants in Aspergillus niger unable to grow on acetate as a sole carbon source were previously isolated by resistance to 1.2% propionate medium containing 0.1% glucose. AcuA mutants lacked acetyl-CoA synthetase (ACS) activity and acuB mutants lacked both ACS and isocitrate lyase activity. An acuA mutant was transforme...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Papadopoulou S,Sealy-Lewis HM

    更新日期:1999-09-01 00:00:00

  • Influence of clindamycin on the stability of coa and fnbB transcripts and adherence properties of Staphylococcus aureus Newman.

    abstract::We investigated whether a subinhibitory concentration of clindamycin (Cli), corresponding to 1/2 the strain-specific minimum inhibitory concentration (MIC), could affect expression and stability of transcripts from genes coding for specific adhesins such as fibronectin binding proteins A (fnbA) and B (fnbB) as well as...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Blickwede M,Wolz C,Valentin-Weigand P,Schwarz S

    更新日期:2005-11-01 00:00:00

  • The pWW0 plasmid imposes a stochastic expression regime to the chromosomal ortho pathway for benzoate metabolism in Pseudomonas putida.

    abstract::Environmental plasmids often expand the metabolic repertoire of bacteria that carry them, but they also interfere with the biochemical and genetic network of the host. The pWW0 plasmid born by Pseudomonas putida mt-2 encodes the TOL pathway for degradation of toluene/m-xylene through production of intermediate compoun...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Silva-Rocha R,de Lorenzo V

    更新日期:2014-07-01 00:00:00

  • Cellular transformation by human T-cell leukemia virus type I.

    abstract::Human T-cell leukemia virus type I (HTLV-I) is an oncogenic retrovirus that was isolated in 1980 from a patient with adult T-cell leukemia. From the numerous experiments using infected patient T-cells, transgenic mice and tissue culture transformation assays, the Tax protein has been determined to be the transforming ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Ressler S,Connor LM,Marriott SJ

    更新日期:1996-07-01 00:00:00

  • PCR detection of Salmonella spp. using primers targeting the quorum sensing gene sdiA.

    abstract::Bacteria communicate with one another and with their host using chemical signalling molecules. This phenomenon is generally described as quorum sensing. A set of primers for PCR detection of Salmonella spp. has been designed using as target the sdiA gene which encodes a signal receptor of the LuxR family. The PCR prod...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Halatsi K,Oikonomou I,Lambiri M,Mandilara G,Vatopoulos A,Kyriacou A

    更新日期:2006-06-01 00:00:00

  • Response regulator, VemR, positively regulates the virulence and adaptation of Xanthomonas campestris pv. campestris.

    abstract::Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. The synthesis of known virulence factors in this organism, such as extracellular enzymes and biofilm, is strictly regulated in response to environmental stimuli. Two-component signal transduction systems sense e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tao J,He C

    更新日期:2010-03-01 00:00:00

  • Fluorescence in situ hybridisation (FISH)--the next generation.

    abstract::Fluorescence in situ hybridisation (FISH) has become one of the major techniques in environmental microbiology. The original version of this technique often suffered from limited sensitivity due to low target copy number or target inaccessibility. In recent years there have been several developments to amend this prob...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Zwirglmaier K

    更新日期:2005-05-15 00:00:00

  • Plasmid-mediated florfenicol and ceftriaxone resistance encoded by the floR and bla(CMY-2) genes in Salmonella enterica serovars Typhimurium and Newport isolated in the United States.

    abstract::Multidrug resistance plasmids carrying the bla(CMY-2) gene have been identified in Salmonella enterica serovars Typhimurium and Newport from the United States. This gene confers decreased susceptibility to ceftriaxone, and is most often found in strains with concomitant resistance to ampicillin, chloramphenicol, strep...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Doublet B,Carattoli A,Whichard JM,White DG,Baucheron S,Chaslus-Dancla E,Cloeckaert A

    更新日期:2004-04-15 00:00:00