Abstract:
:A Deltalgt (Lgt, lipoprotein diacylglyceryl transferase) isogenic mutant was obtained which indicates that lgt is not essential for cell growth in vitro, like in the Gram-positive bacterium Bacillus subtilis, but unlike in the proteobacteria Escherichia coli and Salmonella typhimurium. The mutation was transduced to a virulent strain. A 5 log attenuation was observed in a respiratory tract model of infection. Metabolic labeling by [U-14C]palmitate revealed the presence of eight to ten lipoproteins in the wild-type strain only, with molecular masses between 15 and 80 kDa. Our findings suggest a major difference in the role of lipoproteins in Gram-positive bacteria versus the proteobacteria.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Petit CM,Brown JR,Ingraham K,Bryant AP,Holmes DJdoi
10.1111/j.1574-6968.2001.tb10720.xkeywords:
subject
Has Abstractpub_date
2001-06-25 00:00:00pages
229-33issue
2eissn
0378-1097issn
1574-6968pii
S0378109701002336journal_volume
200pub_type
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