Rapid characterization of Mycobacterium fortuitum-chelonei complex by restriction fragment length polymorphism of ribosomal RNA genes.

Abstract:

:Using labelled, gamma-32P rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum-chelonei complex was analysed. Each DNA sample was cleaved with EcoRI restriction endonuclease, the fragments were separated by agarose gel electrophoresis and transferred to nitrocellulose membrane. Fragments of DNA containing rRNA genes were identified by hybridization with gamma-32P-labelled rRNA. Patterns were found to be species specific and both the species were distinguishable from each other. Results indicate that this approach can be used for rapid genomic characterization of the Mycobacterium fortuitum-chelonei complex.

journal_name

FEMS Microbiol Lett

authors

Kanaujia GV,Katoch VM,Shivannavar CT,Sharma VD,Patil MA

doi

10.1016/0378-1097(91)90552-l

subject

Has Abstract

pub_date

1991-01-15 00:00:00

pages

205-8

issue

2-3

eissn

0378-1097

issn

1574-6968

journal_volume

61

pub_type

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