A leader open reading frame is essential for the expression in Escherichia coli of GC-rich leuB gene of an extreme thermophile, Thermus thermophilus.


:To improve expression efficiency of the leuB gene of an extreme thermophile. Thermus thermophilus, in Escherichia coli, the gene was placed under a potent promoter, tac. However, the expression was hardly improved, despite increased transcription. The expression under tac promoter was significantly improved by introducing a leader open reading frame in front of the gene. Similar improvement under a weak promoter, tet, with a leader open reading frame had been described previously. The present results provide evidence that the major limiting step in the expression of a GC-rich thermophile gene in E. coli is translation, and that the addition of a leader open reading frame is more crucial for high level expression of the gene than the use of a potent promoter.


FEMS Microbiol Lett


Ishida M,Oshima T




Has Abstract


1996-01-01 00:00:00














  • Autocrine response of Schizosaccharomyces pombe haploid cells to mating pheromones.

    abstract::The mating response of the fission yeast Schizosaccharomyces pombe is mediated by mating pheromones, M-factor and P-factor, produced by h- and h+ cells, respectively. When the M-factor receptor (Map3) was ectopically expressed in h- cells lacking the P-factor receptor (Mam2), they acquired mating competence in respons...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kitamura K,Nakamura T,Miki F,Shimoda C

    更新日期:1996-09-15 00:00:00

  • Functional characterisation of the chaperones DnaK, DnaJ, and GrpE from Clostridium acetobutylicum.

    abstract::The DnaK chaperone system is involved in various cellular processes such as the control of the folded and oligomeric state of proteins under stress and non-stress conditions. In this study we functionally characterised the homologues of the DnaK system from Clostridium acetobutylicum DnaK, DnaJ, GrpE and OrfA were het...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rüngeling E,Laufen T,Bahl H

    更新日期:1999-01-01 00:00:00

  • Adherence of Candida albicans to components of the subendothelial extracellular matrix.

    abstract::Candida albicans yeasts adhered avidly to extracellular matrix (ECM) proteins, type IV collagen, laminin, and fibronectin immobilized on plastic. Type IV collagen showed an increase of adherence of 400% above control values; laminin, 300%; and fibronectin, 150%. In addition, all three (in quantities of 0.02-200 microg...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Klotz SA

    更新日期:1990-03-15 00:00:00

  • tmRNA--a novel high-copy-number RNA diagnostic target--its application for Staphylococcus aureus detection using real-time NASBA.

    abstract::A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 other bacterial species. A detection limit of 1 cell equivalent wa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: O'Grady J,Lacey K,Glynn B,Smith TJ,Barry T,Maher M

    更新日期:2009-12-01 00:00:00

  • Biochemical, electrophoretic and immunohistochemical aspects of malate dehydrogenase in truffles (Ascomycotina).

    abstract::The malate dehydrogenase (MDH; EC; L-malate-NAD(+)-oxidoreductase) activities of truffles of the genus Tuber (Tuber melanosporum Vittad., Tuber brumale Vittad., Tuber aestivum Vittad., Tuber magnatum Pico, Tuber rufum Pico) have been characterized with regard to the K(m) and V(max) values in the direct and re...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zarivi O,Cesare P,Aimola P,Ragnelli AM,Scirri C,Cimini A,Bonfigli A,Pacioni G,Miranda M

    更新日期:2000-04-15 00:00:00

  • Comparison of cell-specific activity between free-living and attached bacteria using isolates and natural assemblages.

    abstract::Marine snow aggregates are microbial hotspots that support high bacterial abundance and activities. We conducted laboratory experiments to compare cell-specific bacterial protein production (BPP) and protease activity between free-living and attached bacteria. Natural bacterial assemblages attached to model aggregates...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Grossart HP,Tang KW,Kiørboe T,Ploug H

    更新日期:2007-01-01 00:00:00

  • Direct inhibition of cytochrome c-induced caspase activation in vitro by Toxoplasma gondii reveals novel mechanisms of interference with host cell apoptosis.

    abstract::The intracellular parasite Toxoplasma gondii is known to inhibit apoptosis of its host cell. The molecular mechanisms of this interference are, however, not yet completely understood. We show here that viable parasites prominently inhibited the activation of caspase 3/7 induced by cytochrome c, dATP and dithiothreitol...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Keller P,Schaumburg F,Fischer SF,Häcker G,Gross U,Lüder CG

    更新日期:2006-05-01 00:00:00

  • Identification of Pantoea ananatis gene encoding membrane pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase and pqqABCDEF operon essential for PQQ biosynthesis.

    abstract::Pantoea ananatis accumulates gluconate during aerobic growth in the presence of glucose. Computer analysis of the P. ananatis SC17(0) sequenced genome revealed an ORF encoding a homologue (named gcd) of the mGDH (EC apoenzyme from Escherichia coli and a putative pyrroloquinoline quinone (PQQ) biosynthetic o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Andreeva IG,Golubeva LI,Kuvaeva TM,Gak ER,Katashkina JI,Mashko SV

    更新日期:2011-05-01 00:00:00

  • Chimeric Ply187 endolysin kills Staphylococcus aureus more effectively than the parental enzyme.

    abstract::Peptidoglycan hydrolases are an effective new source of antimicrobials. A chimeric fusion protein of the Ply187 endopeptidase domain and LysK SH3b cell wall-binding domain is a potent agent against Staphylococcus aureus in four functional assays. ...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Mao J,Schmelcher M,Harty WJ,Foster-Frey J,Donovan DM

    更新日期:2013-05-01 00:00:00

  • Comparison of Mycoplasma agalactiae isolates by pulsed field gel electrophoresis, SDS-PAGE and immunoblotting.

    abstract::We have analyzed 81 isolates of Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS-PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in M. agalactiae isolates wa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tola S,Idini G,Manunta D,Casciano I,Rocchigiani AM,Angioi A,Leori G

    更新日期:1996-10-01 00:00:00

  • Cloning and partial sequence of transferrin-binding protein 2 of Neisseria meningitidis using a novel method: twin N-terminal PCR.

    abstract::The genes encoding transferrin-binding proteins (TBPs) 1 and 2 of Neisseria meningitidis and N. gonorrhoeae were used as model loci in a novel method of cloning (twin N-terminal polymerase chain reaction; TNT-PCR) involving amplification between the 5' ends of two genes. Primers were based on N-terminal amino-acid seq...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wilton J,Ala'Aldeen D,Palmer HM,Borriello SP

    更新日期:1993-02-15 00:00:00

  • Rapid characterization of Mycobacterium fortuitum-chelonei complex by restriction fragment length polymorphism of ribosomal RNA genes.

    abstract::Using labelled, gamma-32P rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum-chelonei complex was analysed. Each DNA sample was cleaved with EcoRI restriction endonuclease, the fragments were separated by agarose gel electro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kanaujia GV,Katoch VM,Shivannavar CT,Sharma VD,Patil MA

    更新日期:1991-01-15 00:00:00

  • Acid stress response in Helicobacter pylori.

    abstract::To determine the existence of an acid stress response in Helicobacter pylori the global changes in the proteins synthesized by the bacterium when subjected to an acid stress were studied. H. pylori ATCC43504 previously adapted to pH 7 did not show an acid stress response as detected by the two-dimensional electrophore...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Toledo H,Valenzuela M,Rivas A,Jerez CA

    更新日期:2002-07-16 00:00:00

  • The arbuscular mycorrhizal fungus, Glomus irregulare, controls the mycotoxin production of Fusarium sambucinum in the pathogenesis of potato.

    abstract::Trichothecenes are an important family of mycotoxins produced by several species of the genus Fusarium. These fungi cause serious disease on infected plants and postharvest storage of crops, and the toxins can cause health problems for humans and animals. Unfortunately, there are few methods for controlling mycotoxin ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ismail Y,McCormick S,Hijri M

    更新日期:2013-11-01 00:00:00

  • Tetratricopeptide-like repeats in type-III-secretion chaperones and regulators.

    abstract::Efficient type-III secretion depends on cytosolic molecular chaperones, which bind specifically to the translocators and effectors. In the past there has been a tendency to shoe-horn all type-III-secretion chaperones into a single structural and functional class. However, we have shown that the LcrH/SycD-like chaperon...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pallen MJ,Francis MS,Fütterer K

    更新日期:2003-06-06 00:00:00

  • Adenosine kinase-deficient mutant of Saccharomyces cerevisiae.

    abstract::A cordycepin-resistant mutant strain of Saccharomyces cerevisiae (CD-R2) was found to be deficient in adenosine kinase. This mutant accumulated S-adenosylhomocysteine during growth in the presence of exogenous adenosine and it grew in a pseudohyphal manner in the presence of this nucleotide. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Iwashima A,Ogata M,Nosaka K,Nishimura H,Hasegawa T

    更新日期:1995-03-15 00:00:00

  • High abundance and diversity of nitrite-dependent anaerobic methane-oxidizing bacteria in a paddy field profile.

    abstract::The discovery of nitrite-dependent anaerobic methane oxidation (n-damo) mediated by 'Candidatus Methylomirabilis oxyfera' with nitrite and methane as substrates has connected biogeochemical carbon and nitrogen cycles in a new way. The paddy fields often carry substantial methane and nitrate, thus may be a favorable ha...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Zhou L,Wang Y,Long XE,Guo J,Zhu G

    更新日期:2014-11-01 00:00:00

  • Expression of the short fimbriae of Porphyromonas gingivalis is regulated in oral bacterial consortia.

    abstract::The Mfa1 protein of Porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between P. gingivalis and Streptococcus gordonii. We utilized a promoter-lacZ reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. Promoter acti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Park Y,James CE,Yoshimura F,Lamont RJ

    更新日期:2006-09-01 00:00:00

  • Immunogenicity of IMS 1113 plus soluble subunit and chimeric proteins containing Mycoplasma hyopneumoniae P97 C-terminal repeat regions.

    abstract::The surface adhesin P97 mediates the adherence of Mycoplasma hyopneumoniae to swine cilia. Two reiterated repeats R1 and R2 are located at the C-terminus of P97. The purpose of this study was to evaluate the immunogenicity of Montanide adjuvant IMS 1113 plus soluble subunit proteins rR1, rR1R2 and their chimeric forms...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Barate AK,Cho Y,Truong QL,Hahn TW

    更新日期:2014-03-01 00:00:00

  • Overexpression of serine hydroxymethyltransferase from halotolerant cyanobacterium in Escherichia coli results in increased accumulation of choline precursors and enhanced salinity tolerance.

    abstract::Serine hydroxymethyltransferase (SHMT) is a key enzyme in cellular one-carbon pathway and has been studied in many living organisms from bacteria to higher plants and mammals. However, biochemical and molecular characterization of SHMT from photoautotrophic microorganisms remains a challenge. Here, we isolated the SHM...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Waditee-Sirisattha R,Sittipol D,Tanaka Y,Takabe T

    更新日期:2012-08-01 00:00:00

  • Delineation of molecular species of a family of diacyltrehaloses from Mycobacterium fortuitum by mass spectrometry.

    abstract::A family of 2,3-di-O-acyl trehaloses (DAT), previously identified in Mycobacterium fortuitum, was studied by fast-atom bombardment mass spectrometry to establish the combinations of fatty acyl substituents and, hence, to delineate the molecular species there comprised. The mass spectra indicated the possible existence...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ariza MA,Valero-Guillén PL

    更新日期:1994-06-15 00:00:00

  • Mutation of rpoS enhances Pseudomonas sp. KL28 growth at higher concentrations of m-cresol and changes its surface-related phenotypes.

    abstract::A Tn5 transposon mutant was isolated of the alkylphenol degrader Pseudomonas sp. KL28 that showed increased growth at higher levels of m-cresol on solid and in liquid cultures. The transposon was inserted at the 5'-terminus of rpoS, which encodes a stationary-phase sigma factor. When grown on agar plates, the rpoS mut...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yun JI,Cho KM,Kim JK,Lee SO,Cho K,Lee K

    更新日期:2007-04-01 00:00:00

  • Comparative analysis of Methicillin-Resistant Staphylococcus aureus (MRSA) and Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) in community and food of animal origin.

    abstract::The aim of this study was to illustrate the relative pervasiveness of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) in community and food of animal origin and their relationship with other genetic determinants. Staphylococcus aureus isolates were su...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zehra A,Gulzar M,Singh R,Kaur S,Gill JPS

    更新日期:2020-12-22 00:00:00

  • Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays.

    abstract::A successful Staphylococcus aureus vaccine should elicit a long-term antibody response that prevents establishment of the infection. The aim of the present study was to evaluate the functional role of antibodies raised against different S. aureus CP5 vaccines in invasion to bovine mammary epithelial cells (MAC-T) and ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Renna MS,Pereyra EA,Baravalle C,Camussone CM,Dallard BE,Marcipar IS,Calvinho LF

    更新日期:2014-11-01 00:00:00

  • The identification of five genetic loci of Francisella novicida associated with intracellular growth.

    abstract::Five transposon mutants of Francisella novicida were isolated that are compromised in their ability to grow in mouse macrophages in vitro. Sequence analysis of the DNA flanking the transposon insertions identified the genes that were interrupted in these mutants. One of the inactivated loci corresponds to the Francise...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gray CG,Cowley SC,Cheung KK,Nano FE

    更新日期:2002-09-24 00:00:00

  • The F plasmid transfer activator TraJ is a dimeric helix-turn-helix DNA-binding protein.

    abstract::TraJ is an activator of the transfer (tra) operon in the F plasmid that counteracts H-NS silencing at the main transfer promoter (P(Y)). TraJ contains 226 aa (26 670 kDa), not 229 aa as reported previously, and forms homodimers. TraJ binds DNA containing P(Y)in vivo as demonstrated using a chromatin-immunoprecipitatio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rodriguez-Maillard JM,Arutyunov D,Frost LS

    更新日期:2010-09-01 00:00:00

  • Occurrence of aminoglycoside-modifying enzymes among isolates of Escherichia coli exhibiting high levels of aminoglycoside resistance isolated from Korean cattle farms.

    abstract::This study investigated 247 Escherichia coli isolates collected from four cattle farms to characterize aminoglycoside-modifying enzyme (AME) genes, their plasmid replicons and transferability. Out of 247 isolates a high number of isolates (total 202; 81.78%) were found to be resistant to various antibiotics by disc di...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Belaynehe KM,Shin SW,Hong-Tae P,Yoo HS

    更新日期:2017-08-01 00:00:00

  • Purification and properties of a 1,3-beta-glucanase from Penicillium oxalicum autolysates.

    abstract::High 1,3-beta-glucanase activity was detected during autolysis in a culture medium containing Penicillium oxalicum. It was due to the combined action of four enzymes. The purification process for the major enzyme produced a homogeneous band in the SDS polyacrylamide gel that corresponded to a molecular weight of 79,40...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Copa-Patiño JL,Reyes F,Pérez-Leblic MI

    更新日期:1989-12-01 00:00:00

  • Physicochemical modification of the excretion product of Saccharomyces cerevisiae killer strains results in fungicidal activity against Candida albicans and Tricophyton mentagrophytes.

    abstract::It is known that certain yeast strains, so called 'killers', can produce and excrete proteinaceous toxins that can induce death of other sensitive strains. We obtained a stable fungicidal factor (SKF) through concentration and stabilization of the excretion product of certain killer strains of Saccharomyces cerevisiae...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bracesco N,Salvo VA,Carrau FM,Nunes E

    更新日期:2006-03-01 00:00:00

  • Evaluation of T3B fingerprinting for identification of clinical and environmental Sporothrix species.

    abstract::In this study, PCR fingerprinting using the universal primer T3B was applied to distinguish among clinical and environmental species of the Sporothrix complex, Sporothrix brasiliensis, S. globosa, S. mexicana, S. pallida, S. luriei and S. schenckii sensu stricto. The T3B fingerprinting generated clearly distinct bandi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Oliveira MM,Franco-Duarte R,Romeo O,Pais C,Criseo G,Sampaio P,Zancope-Oliveira RM

    更新日期:2015-03-01 00:00:00