A leader open reading frame is essential for the expression in Escherichia coli of GC-rich leuB gene of an extreme thermophile, Thermus thermophilus.

abstract：:The purpose of this study was to generate highly specific serological reagents for the quantitative identification of Actinomyces naeslundii in clinical samples, in particular dental plaque. Balb/c mice were immunized with pasteurized human A. naeslundii strains representing different genospecies and serotypes. Ten hy...

journal_title：FEMS microbiology letters

authors： Thurnheer T,Guggenheim B,Gmür R

更新日期：1997-05-15 00:00:00

abstract：:Biological halogenation of aromatic compounds implies the generation of reducing equivalents in the form of e.g. NADH. Thermodynamic calculations show that coupling the halogenation step to a step in which the reducing equivalents are oxidized with a potent oxidant such as O2 or N2O makes the halogenation reaction the...

journal_title：FEMS microbiology letters

authors： Dolfing J

更新日期：1998-10-15 00:00:00

abstract：:Insertion sequence IS100 was localized on a 9.5-kb plasmid of Yersinia pestis and was shown to be specific for Y. pestis and serotype I strains of Y. pseudotuberculosis. The nucleotide sequence of IS100 isolated from this plasmid was determined. The element, which was flanked by 5-bp direct repeats, contained 1953 bp ...

journal_title：FEMS microbiology letters

authors： Podladchikova ON,Dikhanov GG,Rakin AV,Heesemann J

更新日期：1994-09-01 00:00:00

abstract：:Researchers studying both cellular and viral disease agents in the laboratory have become infected since the early days of microbiology 150 years ago. However, in the early 21st century, new concerns about bioweapons being used to generate terror and also with a series of newly emerging or newly understood disease-cau...

journal_title：FEMS microbiology letters

authors： Silver S

更新日期：2015-01-01 00:00:00

abstract：:Bacillus subtilis possesses two isogenes encoding glutamate racemases, the poly-gamma-glutamate synthesis-linking Glr enzyme and the YrpC isozyme, and produces abundant amounts of the Glr enzyme. The YrpC isozyme, but not the Glr enzyme, was found to influence the activity of DNA gyrase, as did the MurI-type glutamate...

journal_title：FEMS microbiology letters

authors： Ashiuchi M,Kuwana E,Komatsu K,Soda K,Misono H

更新日期：2003-06-27 00:00:00

abstract：:Bacteria are in constant conflict with competing bacterial and eukaryotic cells. To cope with the various challenges, bacteria developed distinct strategies, such as toxins that inhibit the growth or kill rivals of the same ecological niche. In recent years, two toxin systems have been discovered - the type VI secreti...

journal_title：FEMS microbiology letters

authors： Braun V,Patzer SI

更新日期：2013-08-01 00:00:00

abstract：:Piscirickettsia salmonis is the etiological agent of Salmonid Rickettsial Septicemia, a disease affecting salmon aquaculture industry. We analyzed the 16S-23S rDNA spacer region (internal transcribed spacer, ITS) of Chilean P. salmonis isolates LF-89 and EM-90. Two main ITS amplification products were obtained by PCR ...

journal_title：FEMS microbiology letters

authors： Casanova A,Obreque J,Sandino AM,Jashés M

更新日期：2001-04-01 00:00:00

abstract：:A bacterial strain, designated as TSB-6, was isolated from the sediments of a Tantloi (India) hot spring at 65 °C. The strain showed 98% 16S rRNA gene sequence similarity with Anoxybacillus kualawohkensis strain KW12 and was found to grow optimally at 37 °C. However, growing cells, cell suspensions, and cell-free extr...

journal_title：FEMS microbiology letters

authors： Paul SC,Jain P,Mitra J,Dutta S,Bhattacharya P,Bal B,Bhattacharyya D,Das Gupta S,Pal S

更新日期：2012-06-01 00:00:00

abstract：:In our recent screen for soil-induced genes, the expression of andA operon (andAcAdAbAa) for anthranilate catabolism in Burkholderia multivorans ATCC 17616 was found to increase dramatically in a soil sample (Nishiyama et al., Environ Microbiol 12: 2539, 2010). The operon was preceded by andR encoding a putative trans...

journal_title：FEMS microbiology letters

authors： Nishiyama E,Ohtsubo Y,Yamamoto Y,Nagata Y,Tsuda M

更新日期：2012-05-01 00:00:00

abstract：:The chemical and molecular mechanism of toxicity of nitrite towards food-spoilage bacteria such as Clostridium botulinum or Clostridium sporogenes is not well understood. In order to discover the active species and explore its chemistry, a number of compounds related to nitrite were synthesized. Their bacteriocidal ef...

journal_title：FEMS microbiology letters

authors： Cui X,Joannou CL,Hughes MN,Cammack R

更新日期：1992-11-01 00:00:00

abstract：:Saccharomyces cerevisiae genes encoding functions necessary for inhibition by the Pseudomonas syringae pv. syringae cyclic lipodepsipeptide, syringomycin-E, were identified by mutant analyses. Syringomycin-E-resistant mutants were isolated, shown to contain single recessive mutations, and divided into eight gene compl...

journal_title：FEMS microbiology letters

authors： Takemoto JY,Yu Y,Stock SD,Miyakawa T

更新日期：1993-12-15 00:00:00

abstract：:The effect feeding antibiotics has on the bacterial population of the rabbit caecum was investigated. No changes in total volatile fatty acid production or total bacterial counts were observed compared with nonantibiotic treated controls. However, treatment with chlortetracycline resulted in an increase of propionate ...

journal_title：FEMS microbiology letters

authors： Abecia L,Fondevila M,Balcells J,Edwards JE,Newbold CJ,McEwan NR

更新日期：2007-07-01 00:00:00

abstract：:Autophagy, a eukaryotic cellular activity leading to the degradation of cellular components, serves as a defense mechanism against facultative intracellular bacteria as well as a growth niche for the obligate intracellular bacterium Coxiella burnetii. We here demonstrate that the obligate intracellular bacterial patho...

journal_title：FEMS microbiology letters

authors： Pachikara N,Zhang H,Pan Z,Jin S,Fan H

更新日期：2009-03-01 00:00:00

abstract：:Organic acids such as monocarboxylic acids, dicarboxylic acids or even more complex molecules such as sugar acids, have displayed great applicability in the industry as these compounds are used as platform chemicals for polymer, food, agricultural and pharmaceutical sectors. Chemical synthesis of these compounds from ...

journal_title：FEMS microbiology letters

authors： Soares-Silva I,Ribas D,Sousa-Silva M,Azevedo-Silva J,Rendulić T,Casal M

更新日期：2020-08-01 00:00:00

abstract：:The outer membrane proteins of several prominent bacterial pathogens demonstrate substantial variation in their surface antigenic epitopes. To determine if this was also true for Pseudomonas aeruginosa outer membrane protein OprF, gene sequencing of a serotype 5 isolate was performed to permit comparison with the publ...

journal_title：FEMS microbiology letters

authors： Martin NL,Rawling EG,Wong RS,Rosok M,Hancock RE

更新日期：1993-11-01 00:00:00

abstract：:We cloned and characterized the Neisseria meningitidis rfaC gene which encodes an enzyme, alpha-1,5 heptosyltransferase I, involved in the synthesis of the deep-core of the lipooligosaccharide. The N. meningitidis rfaC mutant, obtained by an allelic exchange, produced lipooligosaccharide which migrated faster in sodiu...

journal_title：FEMS microbiology letters

authors： Stojiljkovic I,Hwa V,Larson J,Lin L,So M,Nassif X

更新日期：1997-06-01 00:00:00

abstract：:The mating response of the fission yeast Schizosaccharomyces pombe is mediated by mating pheromones, M-factor and P-factor, produced by h- and h+ cells, respectively. When the M-factor receptor (Map3) was ectopically expressed in h- cells lacking the P-factor receptor (Mam2), they acquired mating competence in respons...

journal_title：FEMS microbiology letters

authors： Kitamura K,Nakamura T,Miki F,Shimoda C

更新日期：1996-09-15 00:00:00

abstract：:A microorganism which reduces Fe(III) during the fermentation of glucose was isolated from freshwater sediment. The Fe(III) was supplied to enrichment cultures as a soluble complex with the bidentate ligand maltol (3-hydroxy-2-methyl-4-pyrone). Advantages that were afforded by the use of Fe(III)(maltol)3 over previous...

journal_title：FEMS microbiology letters

authors： Dobbin PS,Carter JP,Garcia-Salamanca San Juan C,von Hobe M,Powell AK,Richardson DJ

更新日期：1999-07-01 00:00:00

abstract：:A Gram-negative, facultatively anaerobic, motile and slightly curved rod-shaped bacterium (BFLP-4(T)) was isolated from the faeces of wild seahorses (Hippocampus guttulatus) captured in northwest Spain (Toralla, Galicia). Strain BFLP-4(T) grew at 10-35 degrees C and pH 5-9 (optimally at 20 degrees C and pH 7.2) and at...

journal_title：FEMS microbiology letters

authors： Balcázar JL,Pintado J,Planas M

更新日期：2010-06-01 00:00:00

abstract：:Interspecies bacterial communication is mediated by autoinducer-2, whose synthesis depends on luxS. Due to the apparent universality of luxS (present in more than 40 bacterial species), it may have an ancient origin; however, no direct evidence is currently available. We amplified luxS in bacteria isolated from 25- to...

journal_title：FEMS microbiology letters

authors： Santiago-Rodriguez TM,Patrício AR,Rivera JI,Coradin M,Gonzalez A,Tirado G,Cano RJ,Toranzos GA

更新日期：2014-01-01 00:00:00

abstract：:Bacteriophages co-exist and co-evolve with their hosts in natural environments. Virulent phages lyse infected cells through lytic cycles, whereas temperate phages often remain dormant and can undergo lysogenic or lytic cycles. In their lysogenic state, prophages are actually part of the host genome and replicate passi...

journal_title：FEMS microbiology letters

authors： Menouni R,Hutinet G,Petit MA,Ansaldi M

更新日期：2015-01-01 00:00:00

abstract：:Two monoclonal antibodies (G6 and 7B), generated against a 63-kDa stress protein (GSP63) from Neisseria gonorrhoeae strain VP1, were used to investigate the antigenic heterogeneity of GSP63 among the Neisseriaceae and its antigenic relationship with the Hsp60 heat-shock protein family. Immunoblotting experiments demon...

journal_title：FEMS microbiology letters

authors： Pannekoek Y,Dankert J,van Putten JP

更新日期：1992-11-15 00:00:00

abstract：:Serratia entomophila and Serratia proteamaculans cause amber disease of the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). Three genes required for virulence, sepABC, are located on a large plasmid, pADAP. The translated products of the sep genes are members of the toxin complex (Tc) family of insecticid...

journal_title：FEMS microbiology letters

authors： Hurst MR,Jones SM,Tan B,Jackson TA

更新日期：2007-10-01 00:00:00

abstract：:Stenotrophomonas maltophilia is a multidrug-resistant opportunistic pathogen. S. maltophilia quorum-sensing system is mediated by the diffusible signal factor (DSF), which synthesis depends on rpfF. It has been reported that rpfF disruption in S. maltophilia K279a leads to a loss of DSF synthesis, reduced levels of ex...

journal_title：FEMS microbiology letters

authors： Alcaraz E,García C,Friedman L,de Rossi BP

更新日期：2019-03-01 00:00:00

abstract：:Peroxidase-conjugated transferrin was used to detect transferrin receptors both in intact outer membrane vesicles (OMVs) from Neisseria species in a dot blot assay, and in SDS-PAGE-separated OMV proteins after transferring to nitrocellulose membranes. All N. meningitidis strains produced transferrin receptors after cu...

journal_title：FEMS microbiology letters

authors： Ferreirós CM,Criado MT,Pintor M,Ferrón L

更新日期：1991-10-15 00:00:00

abstract：:Using primers corresponding to conserved regions of the bacterial regulatory gene fur, a homologue of this gene from the genome of Rhizobium leguminosarum biovar viciae, the nitrogen-fixing symbiont of peas, was isolated and sequenced. The fur gene is normally expressed constitutively, independent of the presence of F...

journal_title：FEMS microbiology letters

authors： de Luca NG,Wexler M,Pereira MJ,Yeoman KH,Johnston AW

更新日期：1998-11-15 00:00:00

abstract：:We have determined the genetic basis of minocycline resistance in a strain of Streptococcus infantis isolated from a healthy human oral cavity. We demonstrate that tet(S), identical to tet(S) found on the enterococcal conjugative transposon Tn6000, is responsible for the observed resistance. The gene is located on a s...

journal_title：FEMS microbiology letters

authors： Ciric L,Brouwer MS,Mullany P,Roberts AP

更新日期：2014-04-01 00:00:00

abstract：:The mosquitocidal crystal protein, Cry19Aa, from Bacillus thuringiensis ssp. jegathesan, has high toxicity to Anopheles stephensi and Culex pipiens but is less toxic to Aedes aegypti. To study the functional role of putative domain II and surface residues in mosquito toxicity, 16 alanine substitution mutations were in...

journal_title：FEMS microbiology letters

authors： Roh JY,Nair MS,Liu XS,Dean DH

更新日期：2009-06-01 00:00:00

abstract：:Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems and leaves of P. lactiflora were grouped into 16 fungal taxa based on morphological traits a...

journal_title：FEMS microbiology letters

authors： Cheng X,Wei Z,Pu S,Xiang M,Yan A,Zhang Y,Wang X

更新日期：2018-12-01 00:00:00

abstract：:A multiplex-polymerase chain reaction (PCR) assay based on one-step amplification and detection of two different mycobacterial genomic fragments was designed for differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from a 500-bp genomic fragment which is well c...

journal_title：FEMS microbiology letters

authors： Shah DH,Verma R,Bakshi CS,Singh RK

更新日期：2002-08-27 00:00:00