A leader open reading frame is essential for the expression in Escherichia coli of GC-rich leuB gene of an extreme thermophile, Thermus thermophilus.

Abstract:

:To improve expression efficiency of the leuB gene of an extreme thermophile. Thermus thermophilus, in Escherichia coli, the gene was placed under a potent promoter, tac. However, the expression was hardly improved, despite increased transcription. The expression under tac promoter was significantly improved by introducing a leader open reading frame in front of the gene. Similar improvement under a weak promoter, tet, with a leader open reading frame had been described previously. The present results provide evidence that the major limiting step in the expression of a GC-rich thermophile gene in E. coli is translation, and that the addition of a leader open reading frame is more crucial for high level expression of the gene than the use of a potent promoter.

journal_name

FEMS Microbiol Lett

authors

Ishida M,Oshima T

doi

10.1111/j.1574-6968.1996.tb07978.x

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

137-42

issue

1

eissn

0378-1097

issn

1574-6968

pii

0378109795004602

journal_volume

135

pub_type

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