A leader open reading frame is essential for the expression in Escherichia coli of GC-rich leuB gene of an extreme thermophile, Thermus thermophilus.

abstract：:The mating response of the fission yeast Schizosaccharomyces pombe is mediated by mating pheromones, M-factor and P-factor, produced by h- and h+ cells, respectively. When the M-factor receptor (Map3) was ectopically expressed in h- cells lacking the P-factor receptor (Mam2), they acquired mating competence in respons...

journal_title：FEMS microbiology letters

authors： Kitamura K,Nakamura T,Miki F,Shimoda C

更新日期：1996-09-15 00:00:00

abstract：:The DnaK chaperone system is involved in various cellular processes such as the control of the folded and oligomeric state of proteins under stress and non-stress conditions. In this study we functionally characterised the homologues of the DnaK system from Clostridium acetobutylicum DnaK, DnaJ, GrpE and OrfA were het...

journal_title：FEMS microbiology letters

authors： Rüngeling E,Laufen T,Bahl H

更新日期：1999-01-01 00:00:00

abstract：:Candida albicans yeasts adhered avidly to extracellular matrix (ECM) proteins, type IV collagen, laminin, and fibronectin immobilized on plastic. Type IV collagen showed an increase of adherence of 400% above control values; laminin, 300%; and fibronectin, 150%. In addition, all three (in quantities of 0.02-200 microg...

journal_title：FEMS microbiology letters

authors： Klotz SA

更新日期：1990-03-15 00:00:00

abstract：:A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 other bacterial species. A detection limit of 1 cell equivalent wa...

journal_title：FEMS microbiology letters

authors： O'Grady J,Lacey K,Glynn B,Smith TJ,Barry T,Maher M

更新日期：2009-12-01 00:00:00

abstract：:The malate dehydrogenase (MDH; EC 1.1.1.37; L-malate-NAD(+)-oxidoreductase) activities of truffles of the genus Tuber (Tuber melanosporum Vittad., Tuber brumale Vittad., Tuber aestivum Vittad., Tuber magnatum Pico, Tuber rufum Pico) have been characterized with regard to the K(m) and V(max) values in the direct and re...

journal_title：FEMS microbiology letters

authors： Zarivi O,Cesare P,Aimola P,Ragnelli AM,Scirri C,Cimini A,Bonfigli A,Pacioni G,Miranda M

更新日期：2000-04-15 00:00:00

abstract：:Marine snow aggregates are microbial hotspots that support high bacterial abundance and activities. We conducted laboratory experiments to compare cell-specific bacterial protein production (BPP) and protease activity between free-living and attached bacteria. Natural bacterial assemblages attached to model aggregates...

journal_title：FEMS microbiology letters

authors： Grossart HP,Tang KW,Kiørboe T,Ploug H

更新日期：2007-01-01 00:00:00

abstract：:The intracellular parasite Toxoplasma gondii is known to inhibit apoptosis of its host cell. The molecular mechanisms of this interference are, however, not yet completely understood. We show here that viable parasites prominently inhibited the activation of caspase 3/7 induced by cytochrome c, dATP and dithiothreitol...

journal_title：FEMS microbiology letters

authors： Keller P,Schaumburg F,Fischer SF,Häcker G,Gross U,Lüder CG

更新日期：2006-05-01 00:00:00

abstract：:Pantoea ananatis accumulates gluconate during aerobic growth in the presence of glucose. Computer analysis of the P. ananatis SC17(0) sequenced genome revealed an ORF encoding a homologue (named gcd) of the mGDH (EC 1.1.99.17) apoenzyme from Escherichia coli and a putative pyrroloquinoline quinone (PQQ) biosynthetic o...

journal_title：FEMS microbiology letters

authors： Andreeva IG,Golubeva LI,Kuvaeva TM,Gak ER,Katashkina JI,Mashko SV

更新日期：2011-05-01 00:00:00

abstract：:Peptidoglycan hydrolases are an effective new source of antimicrobials. A chimeric fusion protein of the Ply187 endopeptidase domain and LysK SH3b cell wall-binding domain is a potent agent against Staphylococcus aureus in four functional assays. ...

journal_title：FEMS microbiology letters

authors： Mao J,Schmelcher M,Harty WJ,Foster-Frey J,Donovan DM

更新日期：2013-05-01 00:00:00

abstract：:We have analyzed 81 isolates of Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS-PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in M. agalactiae isolates wa...

journal_title：FEMS microbiology letters

authors： Tola S,Idini G,Manunta D,Casciano I,Rocchigiani AM,Angioi A,Leori G

更新日期：1996-10-01 00:00:00

abstract：:The genes encoding transferrin-binding proteins (TBPs) 1 and 2 of Neisseria meningitidis and N. gonorrhoeae were used as model loci in a novel method of cloning (twin N-terminal polymerase chain reaction; TNT-PCR) involving amplification between the 5' ends of two genes. Primers were based on N-terminal amino-acid seq...

journal_title：FEMS microbiology letters

authors： Wilton J,Ala'Aldeen D,Palmer HM,Borriello SP

更新日期：1993-02-15 00:00:00

abstract：:Using labelled, gamma-32P rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum-chelonei complex was analysed. Each DNA sample was cleaved with EcoRI restriction endonuclease, the fragments were separated by agarose gel electro...

journal_title：FEMS microbiology letters

authors： Kanaujia GV,Katoch VM,Shivannavar CT,Sharma VD,Patil MA

更新日期：1991-01-15 00:00:00

abstract：:To determine the existence of an acid stress response in Helicobacter pylori the global changes in the proteins synthesized by the bacterium when subjected to an acid stress were studied. H. pylori ATCC43504 previously adapted to pH 7 did not show an acid stress response as detected by the two-dimensional electrophore...

journal_title：FEMS microbiology letters

authors： Toledo H,Valenzuela M,Rivas A,Jerez CA

更新日期：2002-07-16 00:00:00

abstract：:Trichothecenes are an important family of mycotoxins produced by several species of the genus Fusarium. These fungi cause serious disease on infected plants and postharvest storage of crops, and the toxins can cause health problems for humans and animals. Unfortunately, there are few methods for controlling mycotoxin ...

journal_title：FEMS microbiology letters

authors： Ismail Y,McCormick S,Hijri M

更新日期：2013-11-01 00:00:00

abstract：:Efficient type-III secretion depends on cytosolic molecular chaperones, which bind specifically to the translocators and effectors. In the past there has been a tendency to shoe-horn all type-III-secretion chaperones into a single structural and functional class. However, we have shown that the LcrH/SycD-like chaperon...

journal_title：FEMS microbiology letters

authors： Pallen MJ,Francis MS,Fütterer K

更新日期：2003-06-06 00:00:00

abstract：:A cordycepin-resistant mutant strain of Saccharomyces cerevisiae (CD-R2) was found to be deficient in adenosine kinase. This mutant accumulated S-adenosylhomocysteine during growth in the presence of exogenous adenosine and it grew in a pseudohyphal manner in the presence of this nucleotide. ...

journal_title：FEMS microbiology letters

authors： Iwashima A,Ogata M,Nosaka K,Nishimura H,Hasegawa T

更新日期：1995-03-15 00:00:00

abstract：:The discovery of nitrite-dependent anaerobic methane oxidation (n-damo) mediated by 'Candidatus Methylomirabilis oxyfera' with nitrite and methane as substrates has connected biogeochemical carbon and nitrogen cycles in a new way. The paddy fields often carry substantial methane and nitrate, thus may be a favorable ha...

journal_title：FEMS microbiology letters

authors： Zhou L,Wang Y,Long XE,Guo J,Zhu G

更新日期：2014-11-01 00:00:00

abstract：:The Mfa1 protein of Porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between P. gingivalis and Streptococcus gordonii. We utilized a promoter-lacZ reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. Promoter acti...

journal_title：FEMS microbiology letters

authors： Park Y,James CE,Yoshimura F,Lamont RJ

更新日期：2006-09-01 00:00:00

abstract：:The surface adhesin P97 mediates the adherence of Mycoplasma hyopneumoniae to swine cilia. Two reiterated repeats R1 and R2 are located at the C-terminus of P97. The purpose of this study was to evaluate the immunogenicity of Montanide adjuvant IMS 1113 plus soluble subunit proteins rR1, rR1R2 and their chimeric forms...

journal_title：FEMS microbiology letters

authors： Barate AK,Cho Y,Truong QL,Hahn TW

更新日期：2014-03-01 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) is a key enzyme in cellular one-carbon pathway and has been studied in many living organisms from bacteria to higher plants and mammals. However, biochemical and molecular characterization of SHMT from photoautotrophic microorganisms remains a challenge. Here, we isolated the SHM...

journal_title：FEMS microbiology letters

authors： Waditee-Sirisattha R,Sittipol D,Tanaka Y,Takabe T

更新日期：2012-08-01 00:00:00

abstract：:A family of 2,3-di-O-acyl trehaloses (DAT), previously identified in Mycobacterium fortuitum, was studied by fast-atom bombardment mass spectrometry to establish the combinations of fatty acyl substituents and, hence, to delineate the molecular species there comprised. The mass spectra indicated the possible existence...

journal_title：FEMS microbiology letters

authors： Ariza MA,Valero-Guillén PL

更新日期：1994-06-15 00:00:00

abstract：:A Tn5 transposon mutant was isolated of the alkylphenol degrader Pseudomonas sp. KL28 that showed increased growth at higher levels of m-cresol on solid and in liquid cultures. The transposon was inserted at the 5'-terminus of rpoS, which encodes a stationary-phase sigma factor. When grown on agar plates, the rpoS mut...

journal_title：FEMS microbiology letters

authors： Yun JI,Cho KM,Kim JK,Lee SO,Cho K,Lee K

更新日期：2007-04-01 00:00:00

abstract：:The aim of this study was to illustrate the relative pervasiveness of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) in community and food of animal origin and their relationship with other genetic determinants. Staphylococcus aureus isolates were su...

journal_title：FEMS microbiology letters

authors： Zehra A,Gulzar M,Singh R,Kaur S,Gill JPS

更新日期：2020-12-22 00:00:00

abstract：:A successful Staphylococcus aureus vaccine should elicit a long-term antibody response that prevents establishment of the infection. The aim of the present study was to evaluate the functional role of antibodies raised against different S. aureus CP5 vaccines in invasion to bovine mammary epithelial cells (MAC-T) and ...

journal_title：FEMS microbiology letters

authors： Renna MS,Pereyra EA,Baravalle C,Camussone CM,Dallard BE,Marcipar IS,Calvinho LF

更新日期：2014-11-01 00:00:00

abstract：:Five transposon mutants of Francisella novicida were isolated that are compromised in their ability to grow in mouse macrophages in vitro. Sequence analysis of the DNA flanking the transposon insertions identified the genes that were interrupted in these mutants. One of the inactivated loci corresponds to the Francise...

journal_title：FEMS microbiology letters

authors： Gray CG,Cowley SC,Cheung KK,Nano FE

更新日期：2002-09-24 00:00:00

abstract：:TraJ is an activator of the transfer (tra) operon in the F plasmid that counteracts H-NS silencing at the main transfer promoter (P(Y)). TraJ contains 226 aa (26 670 kDa), not 229 aa as reported previously, and forms homodimers. TraJ binds DNA containing P(Y)in vivo as demonstrated using a chromatin-immunoprecipitatio...

journal_title：FEMS microbiology letters

authors： Rodriguez-Maillard JM,Arutyunov D,Frost LS

更新日期：2010-09-01 00:00:00

abstract：:This study investigated 247 Escherichia coli isolates collected from four cattle farms to characterize aminoglycoside-modifying enzyme (AME) genes, their plasmid replicons and transferability. Out of 247 isolates a high number of isolates (total 202; 81.78%) were found to be resistant to various antibiotics by disc di...

journal_title：FEMS microbiology letters

authors： Belaynehe KM,Shin SW,Hong-Tae P,Yoo HS

更新日期：2017-08-01 00:00:00

abstract：:High 1,3-beta-glucanase activity was detected during autolysis in a culture medium containing Penicillium oxalicum. It was due to the combined action of four enzymes. The purification process for the major enzyme produced a homogeneous band in the SDS polyacrylamide gel that corresponded to a molecular weight of 79,40...

journal_title：FEMS microbiology letters

authors： Copa-Patiño JL,Reyes F,Pérez-Leblic MI

更新日期：1989-12-01 00:00:00

abstract：:It is known that certain yeast strains, so called 'killers', can produce and excrete proteinaceous toxins that can induce death of other sensitive strains. We obtained a stable fungicidal factor (SKF) through concentration and stabilization of the excretion product of certain killer strains of Saccharomyces cerevisiae...

journal_title：FEMS microbiology letters

authors： Bracesco N,Salvo VA,Carrau FM,Nunes E

更新日期：2006-03-01 00:00:00

abstract：:In this study, PCR fingerprinting using the universal primer T3B was applied to distinguish among clinical and environmental species of the Sporothrix complex, Sporothrix brasiliensis, S. globosa, S. mexicana, S. pallida, S. luriei and S. schenckii sensu stricto. The T3B fingerprinting generated clearly distinct bandi...

journal_title：FEMS microbiology letters

authors： Oliveira MM,Franco-Duarte R,Romeo O,Pais C,Criseo G,Sampaio P,Zancope-Oliveira RM

更新日期：2015-03-01 00:00:00