A leader open reading frame is essential for the expression in Escherichia coli of GC-rich leuB gene of an extreme thermophile, Thermus thermophilus.


:To improve expression efficiency of the leuB gene of an extreme thermophile. Thermus thermophilus, in Escherichia coli, the gene was placed under a potent promoter, tac. However, the expression was hardly improved, despite increased transcription. The expression under tac promoter was significantly improved by introducing a leader open reading frame in front of the gene. Similar improvement under a weak promoter, tet, with a leader open reading frame had been described previously. The present results provide evidence that the major limiting step in the expression of a GC-rich thermophile gene in E. coli is translation, and that the addition of a leader open reading frame is more crucial for high level expression of the gene than the use of a potent promoter.


FEMS Microbiol Lett


Ishida M,Oshima T




Has Abstract


1996-01-01 00:00:00














  • Conserved motifs in the CRISPR leader sequence control spacer acquisition levels in Type I-D CRISPR-Cas systems.

    abstract::Integrating short DNA fragments at the correct leader-repeat junction is key to successful CRISPR-Cas memory formation. The Cas1-2 proteins are responsible to carry out this process. However, the CRISPR adaptation process additionally requires a DNA element adjacent to the CRISPR array, called leader, to facilitate ef...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kieper SN,Almendros C,Brouns SJJ

    更新日期:2019-06-01 00:00:00

  • Different substrate specificities of two triazine hydrolases (TrzNs) from Nocardioides species.

    abstract::Nocardioides sp. strain MTD22 degraded atrazine, ametryn and atraton, as did Arthrobacter aurescens strain TC1 and Nocardioides sp. strain C190. These strains contain trzN, a gene coding for TrzN, triazine hydrolase showing a broad substrate range. However, Nocardioides sp. strain AN3 degraded only atrazine despite co...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yamazaki K,Fujii K,Iwasaki A,Takagi K,Satsuma K,Harada N,Uchimura T

    更新日期:2008-09-01 00:00:00

  • Expression of the landomycin biosynthetic gene cluster in a PKS mutant of Streptomyces fradiae is dependent on the coexpression of a putative transcriptional activator gene.

    abstract::The formation of landomycin A or one of its derivatives (5,6-anhydrolandomycin A) in a heterologous strain has never been achieved. It has now been made possible by the coexpression of a cosmid containing all biosynthetic genes necessary to produce landomycin A together with a pathway-specific regulatory gene. As host...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: von Mulert U,Luzhetskyy A,Hofmann C,Mayer A,Bechthold A

    更新日期:2004-01-15 00:00:00

  • The structural gene for aspartokinase II in Bacillus subtilis is closely linked to the sdh operon.

    abstract::The aecA and aecB loci map at 250 and 290 degrees, respectively, on the Bacillus subtilis chromosomal genetic map. The aecB locus has been proposed as the structural gene for aspartokinase II. From DNA sequence analyses and comparisons to the sequence of the aspartokinase II gene, it can be concluded that the structur...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Petricek M,Rutberg L,Hederstedt L

    更新日期:1989-10-01 00:00:00

  • Evaluating efficacy of bacteriophage therapy against Staphylococcus aureus infections using a silkworm larval infection model.

    abstract::Silkworm larva has recently been recognized as an alternative model animal for higher mammals to evaluate the effects of antibiotics. In this study, we examined the efficacy of the bacteriophage (phage) therapy, which harnesses phages as antibacterial agents, against Staphylococcus aureus infections, using the silkwor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Takemura-Uchiyama I,Uchiyama J,Kato S,Inoue T,Ujihara T,Ohara N,Daibata M,Matsuzaki S

    更新日期:2013-10-01 00:00:00

  • DNA from Aspergillus flavus contains 5-methylcytosine.

    abstract::DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by us...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gowher H,Ehrlich KC,Jeltsch A

    更新日期:2001-11-27 00:00:00

  • Direct inhibition of cytochrome c-induced caspase activation in vitro by Toxoplasma gondii reveals novel mechanisms of interference with host cell apoptosis.

    abstract::The intracellular parasite Toxoplasma gondii is known to inhibit apoptosis of its host cell. The molecular mechanisms of this interference are, however, not yet completely understood. We show here that viable parasites prominently inhibited the activation of caspase 3/7 induced by cytochrome c, dATP and dithiothreitol...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Keller P,Schaumburg F,Fischer SF,Häcker G,Gross U,Lüder CG

    更新日期:2006-05-01 00:00:00

  • An Escherichia coli-Enterococcus faecalis shuttle vector as a tool for the construction of a group B Streptococcus heterologous mutant expressing the beta antigen (Bac) of the C protein complex.

    abstract::Group B streptococci (GBS) represent a very important group of human pathogens. So far little is known about the mechanisms by which these bacteria can cause disease and the bacterial factors involved. One putative virulence factor is the beta antigen of the C protein complex (Bac), which can bind to the Fc region of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kreikemeyer B,Jerlström PG

    更新日期:1999-11-15 00:00:00

  • The oxyR gene from Erwinia carotovora: cloning, sequence analysis and expression in Escherichia coli.

    abstract::Homologs of the Escherichia coli oxyR gene were identified in several Erwinia species, using a combination of PCR and Southern hybridization analysis. The oxyR gene from Erwinia carotovora was isolated on a cosmid clone and characterized. The gene and deduced gene product shared high level sequence identity with their...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Calcutt MJ,Lewis MS,Eisenstark A

    更新日期:1998-10-15 00:00:00

  • Transcriptional analysis of the sigA and sigB genes of Brevibacterium lactofermentum.

    abstract::Transcription of the sigA and sigB genes of Brevibacterium lactofermentum, encoding the principal sigma factors of RNA polymerase, has been investigated by Northern blot and primer extension analysis. Northern hybridizations revealed that sigA is transcribed as a monocistronic mRNA of 1.7 kb and sigB gives two transcr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Oguiza JA,Marcos AT,Martín JF

    更新日期:1997-08-01 00:00:00

  • Biosynthesis of glyantrypine by Aspergillus clavatus.

    abstract::The biosynthesis of glyantrypine from radiolabelled amino acid precursors has been shown experimentally to involve anthranilic acid, tryptophan and glycine. Low values for percentage incorporation of radiolabel into glyantrypine were partly influenced by a complex array of other novel alkaloids shown by the radiolabel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Penn J,Purcell M,Mantle PG

    更新日期:1992-05-01 00:00:00

  • Spiroethers of German chamomile inhibit production of aflatoxin G and trichothecene mycotoxin by inhibiting cytochrome P450 monooxygenases involved in their biosynthesis.

    abstract::The essential oil of German chamomile showed specific inhibition toward aflatoxin G(1) (AFG(1)) production, and (E)- and (Z)-spiroethers were isolated as the active compounds from the oil. The (E)- and (Z)-spiroethers inhibited AFG(1) production of Aspergillus parasiticus with inhibitory concentration 50% (IC(50)) val...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yoshinari T,Yaguchi A,Takahashi-Ando N,Kimura M,Takahashi H,Nakajima T,Sugita-Konishi Y,Nagasawa H,Sakuda S

    更新日期:2008-07-01 00:00:00

  • Sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family Sphingomonadaceae isolated from Misasa, Tottori, Japan.

    abstract::A red-pigmented, Gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (TDMA-17(T)) was isolated from fresh water collected at Misasa, a radioactive site in Japan. TDMA-17(T) was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g(-1) dry c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Asker D,Beppu T,Ueda K

    更新日期:2007-08-01 00:00:00

  • Environmental adaptation factors of two yeasts isolated from the leachate of a uranium mineral heap.

    abstract::Two yeasts from the effluent of a uranium mineral heap were identified as Candida sorbophila and Rhodotorula mucilaginosa. They are well adapted to such an environment as shown by their tolerance to metals and acid pH. However, different mechanisms could be involved in the adaptation: C. sorbophila could be considered...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: de Silóniz MI,Payo EM,Callejo MA,Marquina D,Peinado JM

    更新日期:2002-05-07 00:00:00

  • A chemiluminescence immunoassay for evaluation of Cryptosporidium parvum growth in vitro.

    abstract::A chemiluminescence immunoassay (CLIA) was developed to detect Cryptosporidium parvum growth in Madin-Darby canine kidney (MDCK) cell cultures. Optimal results were obtained when MDCK cells were plated at a density of 1 x 10(4) cells/well (96-well plate) and maintained as a monolayer for 4 days prior to infection with...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: You X,Arrowood MJ,Lejkowski M,Xie L,Schinazi RF,Mead JR

    更新日期:1996-03-01 00:00:00

  • STa-induced translocation of protein kinase C from cytosol to membrane in rat enterocytes.

    abstract::Escherichia coli heat stable enterotoxin (STa) binds to isolated rat intestinal epithelial cells and triggers a cascade reaction including increase of intracellular calcium levels ([Ca(2+)](i)) and membrane bound protein kinase C (PKC) activity. In response to STa, the cytosolic PKC activity falls from 110 to 35 nmol ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ganguly U,Chaudhury AG,Basu A,Sen PC

    更新日期:2001-10-16 00:00:00

  • Bacterial alpha-glucan phosphorylases.

    abstract::Although glycogen and other alpha-1,4-D-glucan storage polysaccharides are present in many bacteria, only few glucan phosphorylases from bacteria have been identified and characterised on the protein or gene level. All bacterial phosphorylases follow the same catalytic mechanisms as their plant and vertebrate counterp...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Schinzel R,Nidetzky B

    更新日期:1999-02-15 00:00:00

  • The phylogenetic position of Hydrogenobacter acidophilus based on 16S rRNA sequence analysis.

    abstract::Hydrogenobacter acidophilus strain 3H-1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen-oxidizer isolated from a Japanese solfataric field. Strain 3H-1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H-1, and sequenced the amplification product directly. Phy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shima S,Yanagi M,Saiki H

    更新日期:1994-06-01 00:00:00

  • Attachment of bacteria to the roots of higher plants.

    abstract::Attachment of soil bacteria to plant cells is supposedly the very early step required in plant-microbe interactions. Attachment also is an initial step for the formation of microbial biofilms on plant roots. For the rhizobia-legume symbiosis, various mechanisms and diverse surface molecules of both partners have been ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Rodríguez-Navarro DN,Dardanelli MS,Ruíz-Saínz JE

    更新日期:2007-07-01 00:00:00

  • Conformational plasticity of molecular chaperones involved in periplasmic and outer membrane protein folding.

    abstract::How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Mas G,Hiller S

    更新日期:2018-07-01 00:00:00

  • Ammonia-induced injury in pure cultures and natural populations of coliform bacteria.

    abstract::Ammonia-induced injury was investigated in pure cultures of Escherichia coli and Enterobacter aerogenes, and in natural coliform populations obtained from the oligotrophic Luxapallila and the eutrophic Sunflower Rivers in northern Mississippi. Pure cultures were affected by ammonia exposure as indicated by changes in ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Naundorf G,Aumen NG

    更新日期:1989-11-01 00:00:00

  • Truepera radiovictrix gen. nov., sp. nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov.

    abstract::Two isolates, belonging to a new species of a novel genus of the Phylum "Deinococcus/Thermus ", were recovered from hot spring runoffs on the Island of São Miguel in the Azores. Strains RQ-24(T) and TU-8 are the first cultured representatives of a distinct phylogenetic lineage within this phylum. These strains form or...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Albuquerque L,Simões C,Nobre MF,Pino NM,Battista JR,Silva MT,Rainey FA,da Costa MS

    更新日期:2005-06-15 00:00:00

  • Extracellular proteolytic activation of bacteriolytic peptidoglycan hydrolases of Staphylococcus simulans biovar staphylolyticus.

    abstract::Staphylococcus simulans biovar staphylolyticus secreted two bacteriolytic peptidoglycan hydrolases as proproteins that were activated as they were processed by an extracellular sulphydryl protease. This processing resulted in the production of multiple molecular-mass forms of each enzyme. Cells from early exponential ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Neumann VC,Heath HE,LeBlanc PA,Sloan GL

    更新日期:1993-06-15 00:00:00

  • Mycobacterium marinum biofilm formation reveals cording morphology.

    abstract::Abstract The emergence of the nontuberculosis mycobacteria (NTM) as clinically relevant pathogens has warranted the study of these ubiquitous organisms in the context of their likely environmental niche, the biofilm. We assayed the NTM bacterium Mycobacterium marinum strain 1218R, a fish outbreak isolate, for biofilm ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hall-Stoodley L,Brun OS,Polshyna G,Barker LP

    更新日期:2006-04-01 00:00:00

  • Autocrine response of Schizosaccharomyces pombe haploid cells to mating pheromones.

    abstract::The mating response of the fission yeast Schizosaccharomyces pombe is mediated by mating pheromones, M-factor and P-factor, produced by h- and h+ cells, respectively. When the M-factor receptor (Map3) was ectopically expressed in h- cells lacking the P-factor receptor (Mam2), they acquired mating competence in respons...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kitamura K,Nakamura T,Miki F,Shimoda C

    更新日期:1996-09-15 00:00:00

  • PCR performance of the highly thermostable proof-reading B-type DNA polymerase from Pyrococcus abyssi.

    abstract::DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indicated that Pab PCR perf...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dietrich J,Schmitt P,Zieger M,Preve B,Rolland JL,Chaabihi H,Gueguen Y

    更新日期:2002-11-19 00:00:00

  • Expression of a new porin 'OmpE' by strains of Salmonella enteritidis.

    abstract::Strains of Salmonella enteritidis expressed a novel porin with a subunit size of 35.5 kDa as shown by SDS-PAGE. This protein was expressed as a major outer membrane protein (MOMP) when grown on nutrient agar, McConkey agar or blood agar, or in Tris-succinate medium; but was only produced in trace amounts when strains ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chart H,Frost JA,Rowe B

    更新日期:1993-05-15 00:00:00

  • Genome diversity at the serA-linked capsule locus in Escherichia coli.

    abstract::Individual isolates of Escherichia coli synthesize one of more than 70 chemically distinct polysaccharides which form the capsule. In this article we review the genetics of capsule production in E. coli and highlight what this is beginning to reveal in terms of the genetic basis of the structural diversity of polysacc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Boulnois G,Drake R,Pearce R,Roberts I

    更新日期:1992-12-15 00:00:00

  • Activity of the Streptomyces coelicolor stress-response sigma factor sigmaH is regulated by an anti-sigma factor.

    abstract::The alternative sigma factor sigmaH has been shown to play an important role in stress response and morphological differentiation in Streptomyces coelicolor. Its gene, sigH, is located in an operon with the gene encoding proposed anti-sigma factor UshX, and one of the promoters directing expression of the operon is de...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sevcikova B,Kormanec J

    更新日期:2002-04-09 00:00:00

  • Virulence determinants in enteroaggregative Escherichia coli from North India and their interaction in in vitro organ culture system.

    abstract::Enteroaggregative Escherichia coli (EAEC) is an important diarrhoeal pathogen causing diseases in multiple epidemiological and clinical settings. In developing countries like India, diarrhoeal diseases are one of the major killers among paediatric population and oddly, few studies are available from Indian paediatric ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gupta D,Sharma M,Sarkar S,Thapa BR,Chakraborti A

    更新日期:2016-09-01 00:00:00