Abstract:
:We cloned and characterized the Neisseria meningitidis rfaC gene which encodes an enzyme, alpha-1,5 heptosyltransferase I, involved in the synthesis of the deep-core of the lipooligosaccharide. The N. meningitidis rfaC mutant, obtained by an allelic exchange, produced lipooligosaccharide which migrated faster in sodium dodecyl sulfate-polyacrylamide gel electrophoresis than the lipooligosaccharide isolated from the wild-type N. meningitidis. The N. meningitidis rfaC mutant was not affected by growth in a rich microbiological medium and did not show any defect in adhesion to epithelial cell lines. Conversely, the rfaC mutant was attenuated in the infant rat model of meningococcemia, thus confirming the importance of intact lipooligosaccharide in the virulence of N. meningitidis.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Stojiljkovic I,Hwa V,Larson J,Lin L,So M,Nassif Xdoi
10.1111/j.1574-6968.1997.tb10392.xsubject
Has Abstractpub_date
1997-06-01 00:00:00pages
41-9issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(97)00135-3journal_volume
151pub_type
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