Variability in the distribution and composition of insertion sequence-like elements in strains of Mycoplasma fermentans.

Abstract:

:Mycoplasma fermentans has been reported to be pathogenic for man. All fourteen strains tested contain an insertion sequence-like element (ISLE) which may be present in multiple copies. To determine whether ISLE copies are similarly distributed in different strains of M. fermentans, restriction enzyme digest fragments of genomic DNA from 14 isolates, from a variety of sources, were separated by electrophoresis, blotted and hybridized to a biotin labelled polymerase chain reaction (PCR) amplified fragment of ISLE. A range of patterns was observed suggesting that the element has a tendency to undergo rearrangement within the genome. Analysis of ISLE sequences revealed inter- and intra-strain polymorphisms.

journal_name

FEMS Microbiol Lett

authors

Pitcher D,Hilbocus J

doi

10.1111/j.1574-6968.1998.tb12898.x

subject

Has Abstract

pub_date

1998-03-01 00:00:00

pages

101-9

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(98)00018-4

journal_volume

160

pub_type

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