Development of 16S rRNA gene-targeted primers for detection of archaeal anaerobic methanotrophs (ANMEs).

abstract：:The branched-chain amino acid permease Bap2p is a transport system for leucine, isoleucine, and valine in Saccharomyces cerevisiae, and its synthesis is regulated transcriptionally. However, the downregulation mechanisms of Bap2p have not been established. Here we demonstrate that the C-terminal region of Bap2p plays ...

journal_title：FEMS microbiology letters

authors： Omura F,Kodama Y,Ashikari T

更新日期：2001-01-15 00:00:00

abstract：:The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles...

journal_title：FEMS microbiology letters

authors： Pérez MA,Gallego FJ,Hidalgo P

更新日期：2001-12-18 00:00:00

abstract：:The first prokaryotic urea carboxylase has previously been purified and characterized from Oleomonas sagaranensis. As the results indicated the presence of an ATP-dependent urea degradation pathway in Bacteria, the characterization of the second component of this pathway, allophanate hydrolase, was carried out. The ge...

journal_title：FEMS microbiology letters

authors： Kanamori T,Kanou N,Kusakabe S,Atomi H,Imanaka T

更新日期：2005-04-01 00:00:00

abstract：:Viable counts and activities of sulfate-reducing bacteria were determined in the oral cavities of 12 healthy volunteers. Of these, 10 harboured viable sulfate-reducing bacteria populations. Six separate sites were sampled: the posterior tongue, anterior tongue, mid buccal mucosa, vestibular mucosa, supragingival plaqu...

journal_title：FEMS microbiology letters

authors： Willis CL,Gibson GR,Allison C,Macfarlane S,Holt JS

更新日期：1995-06-15 00:00:00

abstract：:Sugar alcohols (polyols) have important roles as nutrients, anti-freezing agents and scavengers of free radicals in cold-adapted bacteria, but the characteristics of polyol dehydrogenases in cold-adapted bacteria remain largely unknown. In this study, based on the observation that a cold-adapted bacterium Pseudomonas ...

journal_title：FEMS microbiology letters

authors： DangThu Q,Nguyen TT,Jang SH,Lee C

更新日期：2021-01-05 00:00:00

abstract：:The distribution of the antiseptic-resistance genes qacE and qacE delta 1 was studied in a large number of Gram-negative bacteria by a method that included the polymerase chain reaction (PCR). A total of 117 strains of Gram-negative bacteria, isolated from clinical or environmental sources, was used in this analysis. ...

journal_title：FEMS microbiology letters

authors： Kazama H,Hamashima H,Sasatsu M,Arai T

更新日期：1998-02-15 00:00:00

abstract：:The study compared images of mature Streptococcus mutans biofilms captured at increasing magnification to determine which microscopy method is most acceptable for imaging the biofilm topography and the extracellular polymeric substance (EPS). In vitro S. mutans biofilms were imaged using (1) scanning electron microsco...

journal_title：FEMS microbiology letters

authors： Weber K,Delben J,Bromage TG,Duarte S

更新日期：2014-01-01 00:00:00

abstract：:The major heat shock proteins from Thiobacillus ferrooxidans were identified as DnaK and GroEL equivalents by Western blotting and analysis of the N-terminal amino acid sequence of spots isolated from dried 2-D polyacrylamide electrophoresis gels. The T. ferrooxidans chaperonins showed 70% and 80% identity with the Es...

journal_title：FEMS microbiology letters

authors： Varela P,Jerez CA

更新日期：1992-11-01 00:00:00

abstract：:The halophilic actinomycete genus Streptomonospora, forming a distinct branch in the 16S rRNA gene phylogenetic tree adjacent to the genera Nocardiopsis and Thermobifida, was first proposed by Cui et al. to accommodate the species type Streptomonospora salina. During a biodiversity and taxonomic study on halophilic fi...

journal_title：FEMS microbiology letters

authors： Zhi XY,Tang SK,Li WJ,Xu LH,Jiang CL

更新日期：2006-10-01 00:00:00

abstract：:Ammonium transporters form a conserved family of transport proteins and are widely distributed among all domains of life. The genome of Nitrosomonas europaea codes for a single gene (rh1) that belongs to the family of the AMT/Rh ammonium transporters. For the first time, this study provides functional and physiologica...

journal_title：FEMS microbiology letters

authors： Weidinger K,Neuhäuser B,Gilch S,Ludewig U,Meyer O,Schmidt I

更新日期：2007-08-01 00:00:00

abstract：:The genes mphA and mphB encode macrolide 2'-phosphotransferases I and II, respectively, and they confer resistance to macrolide antibiotics in Escherichia coli. To study the expression of these genes in Gram-positive bacteria, we constructed recombinant plasmids that consisted of an mph gene and the pUB110 vector in B...

journal_title：FEMS microbiology letters

authors： Noguchi N,Tamura Y,Katayama J,Narui K

更新日期：1998-02-15 00:00:00

abstract：:In a previous study, we isolated the spaA gene encoding the surface protective antigen A, SpaA, of Erysipelothrix rhusiopathiae, and found that the N-terminal region of SpaA was responsible for protective immunity against erysipelas and that the C-terminal region contained eight repeat units consisting of 20 amino aci...

journal_title：FEMS microbiology letters

authors： Makino SI,Yamamoto K,Asakura H,Shirahata T

更新日期：2000-05-15 00:00:00

abstract：:Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xyl...

journal_title：FEMS microbiology letters

authors： Chávez R,Navarro C,Calderón I,Peirano A,Bull P,Eyzaguirre J

更新日期：2002-07-02 00:00:00

abstract：:Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild...

journal_title：FEMS microbiology letters

authors： Ukibe K,Katsuragi T,Tani Y,Takagi H

更新日期：2008-09-01 00:00:00

abstract：:The influence of extracellular polymeric substances (EPS) on bacterial cell adhesion onto solid surfaces was investigated using 27 heterotrophic bacterial strains isolated from a wastewater treatment reactor. Cell adhesion onto glass beads was carried out by the packed-bed method and the results were discussed in term...

journal_title：FEMS microbiology letters

authors： Tsuneda S,Aikawa H,Hayashi H,Yuasa A,Hirata A

更新日期：2003-06-27 00:00:00

abstract：:mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These st...

journal_title：FEMS microbiology letters

authors： Saito T,Okano K,Park HD,Itayama T,Inamori Y,Neilan BA,Burns BP,Sugiura N

更新日期：2003-12-12 00:00:00

abstract：:Occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in Japan and Korea. The tet(M) gene was detected in 34 Japanese and Korean isolates, which included Vibrio sp., Lactococcu...

journal_title：FEMS microbiology letters

authors： Kim SR,Nonaka L,Suzuki S

更新日期：2004-08-01 00:00:00

abstract：:The aim of this study was to screen two cheese starter cultures and cheese-borne microbial communities with the potential to produce biogenic amines in cheese during ripening. Bacteria of the genera Enterococcus and Lactobacillus and coliform bacteria were isolated from Dutch-type semi-hard cheese at the beginning of ...

journal_title：FEMS microbiology letters

authors： Burdychova R,Komprda T

更新日期：2007-11-01 00:00:00

abstract：:The genes for fructosyltransferase (ftf) and glucan-binding protein (gbp) of Streptococcus mutans strain Ingbritt have been cloned in E. coli using bacteriophage and plasmid vectors. A single ftf gene appears to be responsible for the appearance of several FTF of different electrophoretic mobilities. Despite the fact ...

journal_title：FEMS microbiology letters

authors： Aduse-Opoku J,Gilpin ML,Russell RR

更新日期：1989-06-01 00:00:00

abstract：:The porin-associated protein of Rhodospirillum rubrum FR1 was found to contain a peptidoglycan binding motif. A partial fragment of 179 amino acids, obtained by cleavage of PAP with trypsin, Asp-N protease, and CNBr, was sequenced. Substantial sequence homology was found of the C-terminal part (residues 126-179) of po...

journal_title：FEMS microbiology letters

authors： Neumann U,Schiltz E,Stahl B,Hillenkamp F,Weckesser J

更新日期：1996-04-15 00:00:00

abstract：:In our recent screen for soil-induced genes, the expression of andA operon (andAcAdAbAa) for anthranilate catabolism in Burkholderia multivorans ATCC 17616 was found to increase dramatically in a soil sample (Nishiyama et al., Environ Microbiol 12: 2539, 2010). The operon was preceded by andR encoding a putative trans...

journal_title：FEMS microbiology letters

authors： Nishiyama E,Ohtsubo Y,Yamamoto Y,Nagata Y,Tsuda M

更新日期：2012-05-01 00:00:00

abstract：:We isolated a fungal strain HS-1 utilizing either ethylene glycol dibenzoate or ethyl benzoate as sole source of carbon and energy, and identified it as Aspergillus nomius, based on morphological and rDNA analyses. An enzyme hydrolyzing the esters was purified from the culture supernatant of the strain to an electroph...

journal_title：FEMS microbiology letters

authors： Uchida H,Kurakata Y,Sawamura H,Inamura N,Kotani T,Uwajima T

更新日期：2003-06-06 00:00:00

abstract：:The signal that triggers the expression of the ace operon and, in turn, the transition of central metabolism's architecture from acetogenic to gluconeogenic in Escherichia coli remains elusive despite extensive research both in vivo and in vitro. Here, with the aid of flux analysis together with measurements of the en...

journal_title：FEMS microbiology letters

authors： El-Mansi M,Phue JN,Shiloach J

更新日期：2021-01-08 00:00:00

abstract：:The multiallelic mating type locus B alpha 1 of Schizophyllum commune encodes a pheromone receptor and putative pheromone genes. A comparison of two alleles encoding receptors that share the same specificity B alpha 1 was performed using strains of different geographic origin. The amino acid sequence alignment reveale...

journal_title：FEMS microbiology letters

authors： Wendland J,Kothe E

更新日期：1996-12-15 00:00:00

abstract：:To identify antigens that would improve the accuracy of serological diagnosis of active tuberculosis, we cloned the genes encoding nine potentially immunogenic secreted or surface-associated proteins of Mycobacterium tuberculosis. Recombinant proteins were reacted with sera from HIV-negative individuals with extrapulm...

journal_title：FEMS microbiology letters

authors： Moran AJ,Treit JD,Whitney JL,Abomoelak B,Houghton R,Skeiky YA,Sampaio DP,Badaró R,Nano FE

更新日期：2001-04-20 00:00:00

abstract：:Pyruvate, the key regulator in connection of a variety of metabolic pathways, influences transcription of the Escherichia coli genome through controlling the activity of two pyruvate-sensing two-component systems (TCSs), BtsSR and PyrSR. Previously, we identified the whole set of regulatory targets of PyrSR with low-a...

journal_title：FEMS microbiology letters

authors： Ogasawara H,Ishizuka T,Yamaji K,Kato Y,Shimada T,Ishihama A

更新日期：2019-12-01 00:00:00

abstract：:Brief exposure of Pseudomonas aeruginosa to a temperature of 10 degrees C or lower caused a significant leakage of the periplasmic beta-lactamase into the medium. The extent of leakage increased as the incubation temperature was lowered to 4 degrees C and reached a maximum at 0 degrees C. Cells grown in the presence o...

journal_title：FEMS microbiology letters

authors： Lei Y,Satake S,Ishii J,Nakae T

更新日期：1991-05-15 00:00:00

abstract：:The Tat machinery catalyzes the transport of folded proteins across the cytoplasmic membrane in bacteria and the thylakoid membrane in plants. Transport occurs only in the presence of an electric field (Δψ) and/or a pH (ΔpH) gradient, and thus, Tat transport is considered to be dependent on the proton motive force (pm...

journal_title：FEMS microbiology letters

authors： Hamsanathan S,Musser SM

更新日期：2018-06-01 00:00:00

abstract：:Matrix proteins are the driving force of assembly of enveloped viruses. Their main function is to interact with and polymerize at cellular membranes and link other viral components to the matrix-membrane complex resulting in individual particle shapes and ensuring the integrity of the viral particle. Although matrix p...

journal_title：FEMS microbiology letters

authors： Timmins J,Ruigrok RW,Weissenhorn W

更新日期：2004-04-15 00:00:00

abstract：:Cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. Among the microorganisms studied here, the yeasts (Schwanniomyces occidentalis, Torulopsis candida, Candida tro...

journal_title：FEMS microbiology letters

authors： Dmitriev VV,Crowley D,Rogachevsky VV,Negri CM,Rusakova TG,Kolesnikova SA,Akhmetov LI

更新日期：2011-02-01 00:00:00