Abstract:
:mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These strains possessed mlrA homologues, and the identities of the genes of MD-1 and Y2 with the corresponding MJ-PV exceeded 98% and 84%, respectively. On the other hand, the mlrA gene was not detected in laboratory strains of the closely related Sphingomonas spp. strains employing hemi-nested polymerase chain reaction detection using two primer sets. Although the microcystin-degrading bacteria were closely related strains, they did not cluster together as the same species. We can conclude that the mlrA gene is conserved in three different bacterial species, and it is unique to microcystin degraders but not to the genus Sphingomonas.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Saito T,Okano K,Park HD,Itayama T,Inamori Y,Neilan BA,Burns BP,Sugiura Ndoi
10.1016/S0378-1097(03)00847-4keywords:
subject
Has Abstractpub_date
2003-12-12 00:00:00pages
271-6issue
2eissn
0378-1097issn
1574-6968pii
S0378109703008474journal_volume
229pub_type
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