Biological effects of DNA damage in the hyperthermophilic archaeon Sulfolobus acidocaldarius.

abstract：:Sera, from 17 patients with diarrhoea or haemolytic uraemic syndrome, and six healthy adults, were tested for neutralisation of Vero cytotoxins (VT). For all 17 patients there was evidence of infection with Escherichia coli O157. Sera from two controls but from none of the patients neutralised VT1, although two patien...

journal_title：FEMS microbiology letters

authors： Scotland SM,Said B,Thomas A,Rowe B

更新日期：1994-01-15 00:00:00

abstract：:Effects of the two-component sensor-regulator pairs DctBD and NtrBC upon the expression of a dctA::phoA fusion from Rhizobium meliloti were determined under excess and limiting nitrogen concentrations in Escherichia coli. Results indicated that NtrBC affected transcription from the dctA promoter on a number of regulat...

journal_title：FEMS microbiology letters

authors： Allaway D,Boesten B,O'Gara F

更新日期：1995-05-15 00:00:00

abstract：:The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from t...

journal_title：FEMS microbiology letters

authors： Brans A,Loriaux A,Thamm I,Joris B,Dusart J

更新日期：1997-04-15 00:00:00

abstract：:Arbuscular mycorrhizal fungi (AMF) have been extensively studied in natural and agricultural ecosystems, but little is known about their diversity and community structure in highly petroleum-polluted soils. In this study, we described an unexpected diversity of AMF in a sedimentation basin of a former petrochemical pl...

journal_title：FEMS microbiology letters

authors： de la Providencia IE,Stefani FO,Labridy M,St-Arnaud M,Hijri M

更新日期：2015-06-01 00:00:00

abstract：:The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide...

journal_title：FEMS microbiology letters

authors： Kirimura K,Matsui T,Sugano S,Usami S

更新日期：1996-08-01 00:00:00

abstract：:Mycelium of Pleurotus ostreatus var. florida with a decreased growth rate contained seven double-stranded RNA segments and isometrical virus particles with diameters of 24 and 30 nm. Mycelium with a normal growth rate lacked dsRNA. Protoclones from virus-containing mycelium contained one to seven of these dsRNA segmen...

journal_title：FEMS microbiology letters

authors： van der Lende TR,Harmsen MC,Go SJ,Wessels JG,Wessel JG

更新日期：1995-01-01 00:00:00

abstract：:Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans. The large amounts of protoplasts required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density-gradient centrifuga...

journal_title：FEMS microbiology letters

authors： Vágvölgyi C,Ferenczy L

更新日期：1991-08-15 00:00:00

abstract：:Leflunomide (LFM) is a novel anti-inflammatory and immunosuppressive drug, and inhibits the growth of cytokine-stimulated lymphoid cells in vitro. The effect of LFM on haploid and diploid cells of Saccharomyces cerevisiae was investigated to elucidate the molecular mechanism of action of the drug. Using a halo assay, ...

journal_title：FEMS microbiology letters

authors： Fujimura H

更新日期：2000-10-01 00:00:00

abstract：:An obligately anaerobic spirochete designated strain SEBR 4228T (T = type strain) was isolated from an oil field of Congo, Central Africa. The strain grew optimally with a sodium chloride concentration of 5% (sodium chloride concentration) growth range 1.0-10%) at 37 degrees C (growth temperature range 20-40 degrees C...

journal_title：FEMS microbiology letters

authors： Magot M,Fardeau ML,Arnauld O,Lanau C,Ollivier B,Thomas P,Patel BK

更新日期：1997-10-15 00:00:00

abstract：:We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pair...

journal_title：FEMS microbiology letters

authors： Lightbody KL,Renshaw PS,Collins ML,Wright RL,Hunt DM,Gordon SV,Hewinson RG,Buxton RS,Williamson RA,Carr MD

更新日期：2004-09-01 00:00:00

abstract：:A novel transposon mutagenesis system for the phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X. campestris pv. campestris (Xcc) was developed using a Tn5-based transposome. A highly efficient transformation up to 10(6) transformants per microg transposon DNA was obtained. Southern blot and thermal as...

journal_title：FEMS microbiology letters

authors： Sun Q,Wu W,Qian W,Hu J,Fang R,He C

更新日期：2003-09-12 00:00:00

abstract：:Staphylococcus carnosus 833, inoculated into sausage meat, increased the level of methyl ketones, which contributed to the cured aroma. These ketones can arise from incomplete beta-oxidation followed by two enzymatic activities: a thioesterase and a decarboxylase. In this study we identified the beta-oxidative pathway...

journal_title：FEMS microbiology letters

authors： Engelvin G,Feron G,Perrin C,Mollé D,Talon R

更新日期：2000-09-01 00:00:00

abstract：:Himalaya hosts a unique environment for microbial ecology. The present study aimed to explore the bioplastic producing bacterial communities along altitude gradient of Pangi-Chamba trans-Himalayan region. A total of 411 bacteria were isolated and 70 characterized at the molecular level for potential polyhydroxyalkanoa...

journal_title：FEMS microbiology letters

authors： Kumar V,Thakur V,Ambika,Kumar S,Singh D

更新日期：2018-07-01 00:00:00

abstract：:The Bacteroides fragilis enterotoxin is an extracellular zinc metalloprotease that has been implicated in diarrheal disease of humans and animals. This toxin causes fluid accumulation in intestinal loops and is cytotoxic for HT-29 cells, an intestinal carcinoma cell line. Here we report the cloning and sequencing of t...

journal_title：FEMS microbiology letters

authors： Kling JJ,Wright RL,Moncrief JS,Wilkins TD

更新日期：1997-01-15 00:00:00

abstract：:Mutants in Aspergillus niger unable to grow on acetate as a sole carbon source were previously isolated by resistance to 1.2% propionate medium containing 0.1% glucose. AcuA mutants lacked acetyl-CoA synthetase (ACS) activity and acuB mutants lacked both ACS and isocitrate lyase activity. An acuA mutant was transforme...

journal_title：FEMS microbiology letters

authors： Papadopoulou S,Sealy-Lewis HM

更新日期：1999-09-01 00:00:00

abstract：:We have investigated the mechanisms used by Trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. Haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mM EGTA. Osmotic protection experiments using carbohydrates with different molecular diameters a...

journal_title：FEMS microbiology letters

authors： Fiori PL,Rappelli P,Rocchigiani AM,Cappuccinelli P

更新日期：1993-05-01 00:00:00

abstract：:Dissimilatory metal-reducing bacteria (DMRB), such as Shewanella oneidensis MR-1, are of great interest for their importance in the biogeochemical cycling of metals and utility in biotechnological processes, such as bioremediation and microbial fuel cells. To identify genes necessary for metal reduction, this study co...

journal_title：FEMS microbiology letters

authors： Kouzuma A,Hashimoto K,Watanabe K

更新日期：2012-01-01 00:00:00

abstract：:The glnAntrBC operon of Proteus vulgaris was cloned and heterologously expressed in Escherichia coli. The nucleotide sequence was determined. An open reading frame of 1407 bp was identified as the glnA gene and the deduced amino acid sequence showed 82% identity with the E. coli glutamine synthetase protein. Heterolog...

journal_title：FEMS microbiology letters

authors： Steglitz-Mörsdorf U,Mörsdorf G,Kaltwasser H

更新日期：1993-01-15 00:00:00

abstract：:It is common among intracellular bacterial pathogens to use eukaryotic-like proteins that mimic and manipulate host cellular processes to promote colonization and intracellular survival. Eukaryotic-like proteins are bacterial proteins with domains that are rare in bacteria, and known to function in the context of a eu...

journal_title：FEMS microbiology letters

authors： Frank AC

更新日期：2019-02-01 00:00:00

abstract：:A 3.5-kb native plasmid (pND103) was identified in Streptococcus thermophilus ST2-1. Preliminary sequence analysis indicated that pND103 belongs to group I S. thermophilus plasmids. A region of approximately 2 kb appears to contain three components: a plus origin of replication (ori) typical of plasmids that replicate...

journal_title：FEMS microbiology letters

authors： Su P,Jury K,Allison GE,Wong WY,Kim WS,Liu CQ,Vancov T,Dunn NW

更新日期：2002-10-29 00:00:00

abstract：:Hopanoid fingerprints allowed to differentiate bacteria formerly connected to the genus Pseudomonas. Whereas all strains related to Pseudomonas and Ralstonia were devoid of any detectable hopanoid, these pentacyclic triterpenoids were found in the Burkholderia species and in related soil isolates, which contained as m...

journal_title：FEMS microbiology letters

authors： Cvejic JH,Putra SR,El-Beltagy A,Hattori R,Hattori T,Rohmer M

更新日期：2000-02-15 00:00:00

abstract：:The effects of specific inhibitors of glycoprotein trimming reactions on Junin virus (JV) replication were investigated. Bromoconduritol, an inhibitor of glucosidase II, significantly reduced infective virus production (DE50: 1.1 mM) and viral protein expression. Neither 1-deoxynojirimycin, an inhibitor of both glucos...

journal_title：FEMS microbiology letters

authors： Silber AM,Candurra NA,Damonte EB

更新日期：1993-05-01 00:00:00

abstract：:Endpolygalacturonase I from Stereum purpureum has been identified as a causative substance for the silver-leaf disease in apples. It possesses a unique pro-sequence in the C-terminal region that lacks endpolygalacturonases from any other origin. In this study, we analyzed and compared enzymatic characteristics between...

journal_title：FEMS microbiology letters

authors： Hamada S,Toda K,Ogawa S,Kubota K,Miyairi K

更新日期：2015-09-01 00:00:00

abstract：:Levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032 was expressed with N-terminal fusion of a LacZ-derived secretion motif (TMITNSSSVP) using the lac promoter system in recombinant Escherichia coli JM109 [pUDF-A81]. In flask cultures, recombinant enzyme activity was detected in culture media, and sequ...

journal_title：FEMS microbiology letters

authors： Lee J,Saraswat V,Koh I,Song KB,Park YH,Rhee SK

更新日期：2001-02-20 00:00:00

abstract：:The ability to identify pathogenic organisms rapidly provides significant benefits to clinicians; in particular, with respect to best prescription practices and tracking of recurrent infections. Conventional bioassays require 3-5 days before identification of an organism can be made, thus compromising the effectivenes...

journal_title：FEMS microbiology letters

authors： Jarvis RM,Goodacre R

更新日期：2004-03-19 00:00:00

abstract：:Sequences from the ribosomal DNA ITS regions have shown that there is limited variation among the species in the Trichoderma viride/atroviride/koningii species complex also known as Hypocrea rufa complex, and that infraspecies variation sometimes is larger than interspecies variation. Strains belonging to T. viride, T...

journal_title：FEMS microbiology letters

authors： Lübeck M,Bulat S,Alekhina I,Lieckfeldt E

更新日期：2004-08-15 00:00:00

abstract：:Probiotics are known for their health-promoting abilities through the production of various metabolites, and also benefits are membrane associated. Probiotic Enterococcus faecalis AG5 has been previously assessed for its probiotic properties and this study further strengthens that claim. Strain AG5 was inoculated with...

journal_title：FEMS microbiology letters

authors： Mishra AK,Kumar SS,Ghosh AR

更新日期：2019-02-01 00:00:00

abstract：:Bordetella pertussis is the causative agent of whooping cough. Traditional vaccines against this disease are inherently reactogenic, thus research is currently focussed on the production of less reactive, acellular vaccines. Expression of candidate antigens for these vaccines in Escherichia coli would be preferable, h...

journal_title：FEMS microbiology letters

authors： Smith AM,Yan H,Groves N,Dalla Pozza T,Walker MJ

更新日期：2000-10-15 00:00:00

abstract：:To study the pathogenicity of mouse cytomegalovirus (MCMV) and to identify virulence determinants, we have isolated and phenotypically characterised 25 temperature-sensitive (ts) mutants. Six of these (tsm9, tsm13, tsm20, tsm22, tsm28 and tsm30) failed to replicate in mice and were avirulent. Five mutants (tsm14, tsm1...

journal_title：FEMS microbiology letters

authors： Akel HM,Sweet C

更新日期：1993-11-01 00:00:00

abstract：:A DNA segment located immediately upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7 strain HA1 was cloned and sequenced. This segment contained an open reading frame encoding a predicted protein of 156 amino acids. A database search identified similar open reading frames upstream of the eaeA gene...

journal_title：FEMS microbiology letters

authors： Zhao S,Mitchell SE,Meng J,Doyle MP,Kresovich S

更新日期：1995-11-01 00:00:00