NtrBC-dependent expression from the Rhizobium meliloti dctA promoter in Escherichia coli.

abstract：:Bacillus subtilis strains BS49 and BS34A, both derived from a common ancestor, carry one or more copies of Tn916, an extremely common mobile genetic element capable of transfer to and from a broad range of microorganisms. Here, we report the complete genome sequence of BS49 and the draft genome sequence of BS34A, whic...

journal_title：FEMS microbiology letters

authors： Browne HP,Anvar SY,Frank J,Lawley TD,Roberts AP,Smits WK

更新日期：2015-01-01 00:00:00

abstract：:Vibrio cholerae non-O1 strain N037 produced a hemolysin (NO37-Hly) which was antigenically similar to E1 Tor hemolysin (E1 Tor-Hly) but different in molecular size, hemolytic activity, and glucose binding capacity. In the gene encoding NO37-Hly, a 4-bp insertion into the structural gene for E1 Tor-Hly (hlyA) was found...

journal_title：FEMS microbiology letters

authors： Honma Y,Yamamoto K,Iwanaga M

更新日期：1995-11-01 00:00:00

abstract：:CRISPR-Cas adaptive immune systems protect bacteria from phage predation, and other foreign genetic elements such as plasmids. Significant advances have been made regarding how CRISPR-Cas systems elicit immunity; however, comparatively little is known about their regulation. To study CRISPR-Cas regulation, we describe...

journal_title：FEMS microbiology letters

authors： Hampton HG,Patterson AG,Chang JT,Taylor C,Fineran PC

更新日期：2019-06-01 00:00:00

abstract：:A particular property of Clostridium difficile strain C34 is an insertion of approximately 2 kb in the tcdA-C34 gene that does not hinder expression of a fully active TcdA-C34 molecule. Intoxication with TcdA-C34 induced an arborized appearance in eukaryotic cells (D-type cytopathic effect); intoxication with TcdB-C34...

journal_title：FEMS microbiology letters

authors： Mehlig M,Moos M,Braun V,Kalt B,Mahony DE,von Eichel-Streiber C

更新日期：2001-05-01 00:00:00

abstract：:The major heat shock proteins from Thiobacillus ferrooxidans were identified as DnaK and GroEL equivalents by Western blotting and analysis of the N-terminal amino acid sequence of spots isolated from dried 2-D polyacrylamide electrophoresis gels. The T. ferrooxidans chaperonins showed 70% and 80% identity with the Es...

journal_title：FEMS microbiology letters

authors： Varela P,Jerez CA

更新日期：1992-11-01 00:00:00

abstract：:A smooth colony strain, resistant to phages lambda and P22, was isolated from sewage and identified as Escherichia coli (strain H). Four temperate phages plaquing on strain H were isolated from sewage. The archetype, HK620, does not plaque on strains C and K12 of E. coli nor on the LT2 strain of Salmonella enterica. B...

journal_title：FEMS microbiology letters

authors： Dhillon TS,Poon AP,Chan D,Clark AJ

更新日期：1998-04-01 00:00:00

abstract：:Multidrug resistance plasmids carrying the bla(CMY-2) gene have been identified in Salmonella enterica serovars Typhimurium and Newport from the United States. This gene confers decreased susceptibility to ceftriaxone, and is most often found in strains with concomitant resistance to ampicillin, chloramphenicol, strep...

journal_title：FEMS microbiology letters

authors： Doublet B,Carattoli A,Whichard JM,White DG,Baucheron S,Chaslus-Dancla E,Cloeckaert A

更新日期：2004-04-15 00:00:00

abstract：:A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambda Zap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cl...

journal_title：FEMS microbiology letters

authors： Palmer HM,Powell NB,Ala'Aldeen DA,Wilton J,Borriello SP

更新日期：1993-06-15 00:00:00

abstract：:The enzymic interconversion of Escherichia coli isocitrate dehydrogenase (ICDH) between the catalytically active and inactive forms is mediated through the activities of ICDH-kinase/phosphatase in response to changes in the metabolic environment. In this study, the use of mutant strains devoid of isocitrate lyase (ace...

journal_title：FEMS microbiology letters

authors： el-Mansi EM

更新日期：1998-09-15 00:00:00

abstract：:Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay w...

journal_title：FEMS microbiology letters

authors： Hara-Kudo Y,Yoshino M,Kojima T,Ikedo M

更新日期：2005-12-01 00:00:00

abstract：:Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild...

journal_title：FEMS microbiology letters

authors： Ukibe K,Katsuragi T,Tani Y,Takagi H

更新日期：2008-09-01 00:00:00

abstract：:Autophagy, a eukaryotic cellular activity leading to the degradation of cellular components, serves as a defense mechanism against facultative intracellular bacteria as well as a growth niche for the obligate intracellular bacterium Coxiella burnetii. We here demonstrate that the obligate intracellular bacterial patho...

journal_title：FEMS microbiology letters

authors： Pachikara N,Zhang H,Pan Z,Jin S,Fan H

更新日期：2009-03-01 00:00:00

abstract：:The cryopreservation of an anaerobic rumen fungus, Piromyces communis OTS1, was examined at -84 degrees C using dimethyl sulfoxide, propylene glycol or ethylene glycol as cryoprotectants. Ethylene glycol was the most effective agent, combining high survival and low toxicity, followed by dimethyl sulfoxide and propylen...

journal_title：FEMS microbiology letters

authors： Sakurada M,Tsuzuki Y,Morgavi DP,Tomita Y,Onodera R

更新日期：1995-04-01 00:00:00

abstract：:The gene (open reading frame (ORF) Tm1469, glk) encoding ATP-dependent ROK (repressors, ORFs, sugar kinases) glucokinase (ATP-GLK, EC 2.7.1.2) of the hyperthermophilic bacterium Thermotoga maritima was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparen...

journal_title：FEMS microbiology letters

authors： Hansen T,Schönheit P

更新日期：2003-09-26 00:00:00

abstract：:Rhizobium leguminosarum bv. trifolii rosR gene encodes a transcriptional regulator involved in the positive regulation of exopolysaccharide synthesis. Transcription of rosR is directed by two promoters, distal P1 and proximal P2, of different strengths. We demonstrated that rosR P1 functions as the main promoter and, ...

journal_title：FEMS microbiology letters

authors： Janczarek M,Skorupska A

更新日期：2009-02-01 00:00:00

abstract：:When challenged by stresses such as starvation, the soil bacterium Bacillus subtilis produces an endospore surrounded by a proteinaceous coat composed of >70 proteins that are organized into three main layers: an amorphous undercoat, lightly staining lamellar inner coat and electron-dense outer coat. This coat protect...

journal_title：FEMS microbiology letters

authors： Müllerová D,Krajčíková D,Barák I

更新日期：2009-10-01 00:00:00

abstract：:Outer membrane-associated proteins of 18 kDa were expressed by enteroaggregative Escherichia coli (EAggEC) belonging to serotypes O126:H27 and O44:H18, which hybridized with a probe derived from a plasmid necessary for enteroaggregative adhesion. The 18 kDa proteins expressed by strains of E. coli, belonging to these ...

journal_title：FEMS microbiology letters

authors： Chart H,Smith HR,Rowe B

更新日期：1995-10-01 00:00:00

abstract：:A chemiluminescence immunoassay (CLIA) was developed to detect Cryptosporidium parvum growth in Madin-Darby canine kidney (MDCK) cell cultures. Optimal results were obtained when MDCK cells were plated at a density of 1 x 10(4) cells/well (96-well plate) and maintained as a monolayer for 4 days prior to infection with...

journal_title：FEMS microbiology letters

authors： You X,Arrowood MJ,Lejkowski M,Xie L,Schinazi RF,Mead JR

更新日期：1996-03-01 00:00:00

abstract：:The development of a methodology to identify the origin of fecal pollution is important both for assessing the degree of risk posed to public health and for developing strategies to mitigate the environmental loading of pathogens associated with waterborne disease transmission. Five rep-PCR genomic fingerprinting meth...

journal_title：FEMS microbiology letters

authors： Mohapatra BR,Broersma K,Mazumder A

更新日期：2007-12-01 00:00:00

abstract：:Acinetobacter baumannii strain A148, a clinical isolate resistant to imipenem (MIC = 32 mg l-1), synthesized two beta-lactamases with pIs 6.3 and > 9.2. The pI 6.3 enzyme hydrolyzed the penicillins, including isoxazoylpenicillins, first-, second- and, to a lesser extent, third-generation cephalosporins. It was inhibit...

journal_title：FEMS microbiology letters

authors： Hornstein M,Sautjeau-Rostoker C,Péduzzi J,Vessières A,Hong LT,Barthélémy M,Scavizzi M,Labia R

更新日期：1997-08-15 00:00:00

abstract：:Acidithiobacillus ferrivorans is an acidophile implicated in low-temperature biomining for the recovery of metals from sulfide minerals. Acidithiobacillus ferrivorans obtains its energy from the oxidation of inorganic sulfur compounds, and genes encoding several alternative pathways have been identified. Next-generati...

journal_title：FEMS microbiology letters

authors： Christel S,Fridlund J,Buetti-Dinh A,Buck M,Watkin EL,Dopson M

更新日期：2016-04-01 00:00:00

abstract：:The 42- and 51-kDa protein genes of Bacillus sphaericus 1593 have been subcloned independently downstream from the cytA gene promoter of Bacillus thuringiensis serovar israelensis and introduced into a non-mosquitocidal strain of Bacillus thuringiensis. Consequently, each protein was overproduced and accumulated as in...

journal_title：FEMS microbiology letters

authors： Nicolas L,Nielsen-Leroux C,Charles JF,Delécluse A

更新日期：1993-02-01 00:00:00

abstract：:Burkholderia pseudomallei, a Gram-negative bacterium is found in the soil and water, mainly in Southeast Asia and Northern Australia. It is responsible for melioidosis in human and animals. The bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. The is...

journal_title：FEMS microbiology letters

authors： Lee MA,Liu Y

更新日期：2000-11-01 00:00:00

abstract：:Conditional gene expression systems are useful tools for studying the role of essential or toxic gene products in bacterial systems. There is a paucity of such systems available for use in the mycobacteria. The utility of the Escherichia coli arabinose-inducible system was looked into, since it is tightly controlled i...

journal_title：FEMS microbiology letters

authors： Carroll P,Brown AC,Hartridge AR,Parish T

更新日期：2007-09-01 00:00:00

abstract：:A series of isogenic mutants lacking either the O1 (O-:K66) or K66 (O1:K-) antigens or both (O-:K-), some of which had additional defects in their LPS core polysaccharide was used to examine the interaction between polymorphonuclear leucocytes (PMNLs) and K. pneumoniae serotype O1:K66. In the absence of serum compleme...

journal_title：FEMS microbiology letters

authors： Williams P,Ciurana B,Camprubi S,Tomas JM

更新日期：1990-06-01 00:00:00

abstract：:Two L-methionine-enriched mutants, SN-78 and SE-57, were isolated in a sulphur-deficient medium from the methylotrophic yeast Candida boidinii ICCF26. No significant differences were detected between the L-cysteine pools of the mutants and the wild-type. In mutant strain SE-57, S-adenosylmethionine and ergosterol leve...

journal_title：FEMS microbiology letters

authors： Avram D,Stan R

更新日期：1992-11-01 00:00:00

abstract：:Environmental plasmids often expand the metabolic repertoire of bacteria that carry them, but they also interfere with the biochemical and genetic network of the host. The pWW0 plasmid born by Pseudomonas putida mt-2 encodes the TOL pathway for degradation of toluene/m-xylene through production of intermediate compoun...

journal_title：FEMS microbiology letters

authors： Silva-Rocha R,de Lorenzo V

更新日期：2014-07-01 00:00:00

abstract：:Nitrogen is a key nutrient for land plants and phytoplankton in terrestrial and aquatic ecosystems. The model alga Chlamydomonas reinhardtii can grow efficiently on several inorganic nitrogen sources (e.g. ammonium, nitrate, nitrite) as well as many amino acids. In this study, we show that Chlamydomonas is unable to u...

journal_title：FEMS microbiology letters

authors： Calatrava V,Hom EFY,Llamas Á,Fernández E,Galván A

更新日期：2018-04-01 00:00:00

abstract：:Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis The phage forms round plaques with a matt outline on agar plates containing a B. subtilis ...

journal_title：FEMS microbiology letters

authors： Willms IM,Hertel R

更新日期：2016-10-01 00:00:00

abstract：:The Fe(III) reductase activity was studied in the South African Fe(III)-reducing bacterium, Thermus scotoductus (SA-01). Fractionation studies revealed that the membrane as well as the soluble fraction contained NAD(P)H-dependent Fe(III) reductase activity. The membrane-associated enzyme was solubilized by KCl treatme...

journal_title：FEMS microbiology letters

authors： Möller C,van Heerden E

更新日期：2006-12-01 00:00:00