Aberrant gene for E1 Tor hemolysin from Vibrio cholerae non-O1, N037.

abstract：:In order to obtain functional expression of PHA synthase gene phaC2 from Pseudomonas aeruginosa in Escherichia coli, the coding region of phaC2 was subcloned, including the ribosomal binding site, into pBluescript SK- collinear to the lac promoter. This plasmid pBHR71-C2 enabled functional expression of phaC2 in E. co...

journal_title：FEMS microbiology letters

authors： Qi Q,Rehm BH,Steinbüchel A

更新日期：1997-12-01 00:00:00

abstract：:Weissella cibaria isolated from human saliva produces a soluble glucan that predominantly has alpha-1,6-glucosidic type linkages. Using degenerated primers that were selected based on the amino acid sequences of conserved regions from known glucansucrases, a single 2.7-kb fragment was isolated. In subsequent steps, a ...

journal_title：FEMS microbiology letters

authors： Kang HK,Oh JS,Kim D

更新日期：2009-03-01 00:00:00

abstract：:This study provides a novel qRT-PCR protocol for specific detection and proof of viability of Phytophthora in environmental samples based on differential accumulation of cox II transcripts. Chemical and physical treatments were tested for their ability to induce in vitro the accumulation of cytochrome oxidase genes en...

journal_title：FEMS microbiology letters

authors： Vannini A,Tomassini A,Bruni N,Vettraino AM

更新日期：2014-04-01 00:00:00

abstract：:In Corynebacterium glutamicum the promoter of the araBAD Escherichia coli gene is positively regulated by both arabinose and the araC gene product, as it is the case in the natural host. If the L-arabinose inducer and an active araC gene are present, significant amounts of araBAD promoter expression take place in the ...

journal_title：FEMS microbiology letters

authors： Ben-Samoun K,Leblon G,Reyes O

更新日期：1999-05-01 00:00:00

abstract：:Wood-rotting fungi possess remarkably diverse extracellular oxidation mechanisms, including enzymes, such as laccase and peroxidases, and Fenton chemistry. The ability to biologically drive Fenton chemistry by the redox cycling of quinones has previously been reported to be present in both ecologically diverging main ...

journal_title：FEMS microbiology letters

authors： Krueger MC,Bergmann M,Schlosser D

更新日期：2016-06-01 00:00:00

abstract：:The enteric microogranisms Salmonella, Escherichia coli and Shigella flexneri prefer to grow in neutral pH environments. They nevertheless experience dramatic pH fluctuations in nature and during pathogenesis. In response to environmental encounters with acid, these organisms have evolved complex, inducible acid survi...

journal_title：FEMS microbiology letters

authors： Bearson S,Bearson B,Foster JW

更新日期：1997-02-15 00:00:00

abstract：:We have investigated the iron-dependent regulation of the psbA gene, encoding the enzyme L-ornithine N(5)-oxygenase in the rhizobacterium Pseudomonas B10. We have cloned and characterized a Pseudomonas B10 gene, designated psbS, required for psbA expression. PsbS is endowed with structural and functional features of e...

journal_title：FEMS microbiology letters

authors： Leoni L,Ambrosi C,Petrucca A,Visca P

更新日期：2002-03-05 00:00:00

abstract：:The sacB gene of Bacillus subtilis was successfully applied in various Arthrobacter, Brevibacterium, Corynebacterium and Rhodococcus strains for the isolation of transposable elements. Three different insertion sequence (IS) elements entrapped in sacB were isolated. The IS elements IS-Bl and IS-Cg isolated from Brevib...

journal_title：FEMS microbiology letters

authors： Jäger W,Schäfer A,Kalinowski J,Pühler A

更新日期：1995-02-01 00:00:00

abstract：:A two-step enzymatic synthesis process of 4-hydroxyisoleucine is suggested. In the first step, the aldol condensation of acetaldehyde and alpha-ketobutyrate catalyzed by specific aldolase results in the formation of 4-hydroxy-3-methyl-2-keto-pentanoate (HMKP). In the second step, amination of HMKP by the branched-chai...

journal_title：FEMS microbiology letters

authors： Smirnov SV,Samsonova NN,Novikova AE,Matrosov NG,Rushkevich NY,Kodera T,Ogawa J,Yamanaka H,Shimizu S

更新日期：2007-08-01 00:00:00

abstract：:Acetobacter spp. are used for industrial vinegar production because of their high ability to oxidize ethanol to acetic acid and high resistance to acetic acid. Two-dimensional gel electrophoretic analysis of a soluble fraction of Acetobacter aceti revealed the presence of several proteins whose production was enhanced...

journal_title：FEMS microbiology letters

authors： Nakano S,Fukaya M,Horinouchi S

更新日期：2004-06-15 00:00:00

abstract：:One hundred and ninety-eight clinical isolates of Mycobacterium kansasii collected between 2003 and 2004 in Japan were genotyped by PCR and restriction enzyme analysis (PRA) and 16S-23S internal transcribed spacer (ITS) sequencing. The results demonstrated that clinical isolates of M. kansasii in Japan are almost excl...

journal_title：FEMS microbiology letters

authors： Iwamoto T,Saito H

更新日期：2006-01-01 00:00:00

abstract：:Rifampicin is a principal drug used to combat infections by mycobacteria and related organisms. Most strains of Mycobacterium are able to inactivate this antibiotic by ribosylation via an ADP-ribosylated intermediate. We found that this inactivation was inhibited by light at levels similar to those prevailing in labor...

journal_title：FEMS microbiology letters

authors： Dabbs ER,Quan S

更新日期：2000-01-01 00:00:00

abstract：:A unique genomic DNA fragment was isolated from Nitrosomonas europaea ATCC 19718. Based on the sequence of this fragment, oligonucleotide primers for polymerase chain reaction amplification were prepared which amplify sequences of 775 and 658 bp. The predicted DNA fragments were both amplified from the genome of N. eu...

journal_title：FEMS microbiology letters

authors： Nejidat A,Abeliovich A

更新日期：1994-07-01 00:00:00

abstract：:The assessment of scientific research is essentially based on several metric parameters, the so-termed Impact Factor perhaps being the predominant one. Despite well-founded criticisms and the wide opposition of reputed scientists, this procedure has become a tool of scientific policy, and is applied in editorial proce...

journal_title：FEMS microbiology letters

authors： Argüelles JC,Argüelles-Prieto R

更新日期：2019-06-01 00:00:00

abstract：:The main hypothesis of this work was to evaluate the presence of lactic acid bacteria (LAB) intrinsically resistant to plant essential oils in sage (Salvia officinalis L.) and laurel (Laurus nobilis), for future applications in functional cheese production by addition of aromatic herbs. The effect of the drying proces...

journal_title：FEMS microbiology letters

authors： Gaglio R,Catania P,Orlando S,Vallone M,Moschetti G,Settanni L

更新日期：2020-05-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC) is an important diarrhoeal pathogen causing diseases in multiple epidemiological and clinical settings. In developing countries like India, diarrhoeal diseases are one of the major killers among paediatric population and oddly, few studies are available from Indian paediatric ...

journal_title：FEMS microbiology letters

authors： Gupta D,Sharma M,Sarkar S,Thapa BR,Chakraborti A

更新日期：2016-09-01 00:00:00

abstract：:Biofilms present complex assemblies of micro-organisms attached to surfaces. they are dynamic structures in which various metabolic activities and interactions between the component cells occur. When phage come in contact with biofilms, further interactions occur dependent on the susceptibility of the biofilm bacteria...

journal_title：FEMS microbiology letters

authors： Sutherland IW,Hughes KA,Skillman LC,Tait K

更新日期：2004-03-12 00:00:00

abstract：:We determined the complete nucleotide sequence of the 16341 bp plasmid pHH205 of the extremely halophilic archaeon Halobacterium salinarum J7. The plasmid has a G+C content of 61.1%. A number of direct and inverted repeat sequences were found in pHH205, while no insertion sequences were found. Thirty-eight large open ...

journal_title：FEMS microbiology letters

authors： Ye X,Ou J,Ni L,Shi W,Shen P

更新日期：2003-04-11 00:00:00

abstract：:The molecular genetics of indole-3-acetic (IAA) synthesis and regulation in Azospirillum brasilense was investigated in this study. Tn5 mutagenesis was performed and five mutants with decreased IAA production were isolated. Five Tn5-inserted genes from these mutants were cloned and sequenced. Four genes were reported ...

journal_title：FEMS microbiology letters

authors： Xie B,Xu K,Zhao HX,Chen SF

更新日期：2005-07-01 00:00:00

abstract：:The glycoside hydrolase (GH) 16 family of Coprinopsis cinerea includes 15 members distributed in four subgroups (A1, A2, B and D) by phylogenetic analysis. The expression patterns match well with the requirement of wall-softening in the germination of basidiospores, hyphal growth and branching, primordium formation, s...

journal_title：FEMS microbiology letters

authors： Kang L,Zhu Y,Bai Y,Yuan S

更新日期：2019-04-01 00:00:00

abstract：:The genes mphA and mphB encode macrolide 2'-phosphotransferases I and II, respectively, and they confer resistance to macrolide antibiotics in Escherichia coli. To study the expression of these genes in Gram-positive bacteria, we constructed recombinant plasmids that consisted of an mph gene and the pUB110 vector in B...

journal_title：FEMS microbiology letters

authors： Noguchi N,Tamura Y,Katayama J,Narui K

更新日期：1998-02-15 00:00:00

abstract：:The binding and hemagglutinating activities of the B subunit(s) of the heat-labile enterotoxin (LTh-B) isolated from human enterotoxigenic Escherichia coli were investigated. The binding of 125I-labeled LTh-B to neuraminidase-treated human type B erythrocytes was most effectively inhibited by ganglioside GM1. A number...

journal_title：FEMS microbiology letters

authors： Sugii S,Tsuji T

更新日期：1990-01-01 00:00:00

abstract：:It was shown that virulent Mycobacterium tuberculosis H37Rv induces necrosis of infected RAW264 cells at 24 h post infection while avirulent H37Ra and an attenuated H37Rv mutant that is deficient for RD1 region (H37RvDeltaRD1) cause less necrosis of the infected cells. While H37Rv caused damage of the mitochondrial in...

journal_title：FEMS microbiology letters

authors： Kaku T,Kawamura I,Uchiyama R,Kurenuma T,Mitsuyama M

更新日期：2007-09-01 00:00:00

abstract：:A cordycepin-resistant mutant strain of Saccharomyces cerevisiae (CD-R2) was found to be deficient in adenosine kinase. This mutant accumulated S-adenosylhomocysteine during growth in the presence of exogenous adenosine and it grew in a pseudohyphal manner in the presence of this nucleotide. ...

journal_title：FEMS microbiology letters

authors： Iwashima A,Ogata M,Nosaka K,Nishimura H,Hasegawa T

更新日期：1995-03-15 00:00:00

abstract：:An accurate monitoring of disease progression is important to evaluate disease susceptibility phenotypes. Over the years, Arabidopsis thaliana has become the model species to serve as a host in plant-pathogen interactions. Despite the efforts to study genetic mechanisms of host defense, little efforts are made for a t...

journal_title：FEMS microbiology letters

authors： Brouwer M,Lievens B,Van Hemelrijck W,Van den Ackerveken G,Cammue BP,Thomma BP

更新日期：2003-11-21 00:00:00

abstract：:We present draft genome sequences of three Holospora species, hosted by the ciliate Paramecium caudatum; that is, the macronucleus-specific H. obtusa and the micronucleus-specific H. undulata and H. elegans. We investigate functions of orthologous core genes conserved across the three Holospora species, which may be e...

journal_title：FEMS microbiology letters

authors： Dohra H,Tanaka K,Suzuki T,Fujishima M,Suzuki H

更新日期：2014-10-01 00:00:00

abstract：:Streptococcus intermedius is well known to produce severe infections in various areas of the body. In this study, we evaluated the ability of S. intermedius to utilise human transferrin as a source of iron and investigated the mechanism by which iron can be obtained from this plasma protein. Adding either ferrous sulf...

journal_title：FEMS microbiology letters

authors： Brochu V,Greinier D,Mayrand D

更新日期：1998-09-01 00:00:00

abstract：:An Escherichia coli mutant defective in high-affinity D-ribose transport is able to utilize the sugar as a sole carbon source, suggesting that other transport systems for D-ribose exist. In order to search for such transporters, transposon mutagenesis was carried out in an rbsB-negative strain containing ribokinase (r...

journal_title：FEMS microbiology letters

authors： Song S,Park C

更新日期：1998-06-15 00:00:00

abstract：:Several beta-galactosidase fusion proteins have been constructed containing the entire VP1 protein from foot-and-mouth disease virus (FMDV) [Corchero et al. (1996) J. Biotechnol. in press]. The antigenicity of the major immunodominant site A (13 amino acids in length) within the VP1 protein has been studied in competi...

journal_title：FEMS microbiology letters

authors： Corchero JL,Villaverde A

更新日期：1996-11-15 00:00:00

abstract：:The ecology of aerobic microorganisms is never explored in marine oxygen minimum zone (OMZ) sediments. Here we reveal aerobic bacterial communities along ∼3 m sediment-horizons of the eastern Arabian Sea OMZ. Sulfide-containing sediment-cores retrieved from 530 mbsl (meters beneath the sea-level) and 580 mbsl were exp...

journal_title：FEMS microbiology letters

authors： Bhattacharya S,Roy C,Mandal S,Sarkar J,Rameez MJ,Mondal N,Mapder T,Chatterjee S,Pyne P,Alam M,Haldar PK,Roy R,Fernandes S,Peketi A,Chakraborty R,Mazumdar A,Ghosh W

更新日期：2020-10-16 00:00:00