Influence of lipopolysaccharide chemotype on the interaction between Klebsiella pneumoniae and human polymorphonuclear leucocytes.

Abstract:

:A series of isogenic mutants lacking either the O1 (O-:K66) or K66 (O1:K-) antigens or both (O-:K-), some of which had additional defects in their LPS core polysaccharide was used to examine the interaction between polymorphonuclear leucocytes (PMNLs) and K. pneumoniae serotype O1:K66. In the absence of serum complement, only a O-:K- strain with a deep rough LPS chemotype elicited a PMNL-dependent chemiluminescent (CL) response. However, following opsonization of the non-capsulated strains by complement, the largest CL response was to the O1:K- mutant. This mutant also activated and bound more complement C3 than any of the other encapsulated or non-capsulated strains examined. Despite the surface exposure of smooth and rough LPS in the encapsulated parent and mutant strains, the K66 antigen reduced the binding of C3 and prevented PMNL activation. Both anti-LPS and anti-K66 antibodies, however, stimulated a PMNL-dependent CL response to the K66 bearing strains.

journal_name

FEMS Microbiol Lett

authors

Williams P,Ciurana B,Camprubi S,Tomas JM

doi

10.1016/0378-1097(90)90085-5

subject

Has Abstract

pub_date

1990-06-01 00:00:00

pages

305-9

issue

3

eissn

0378-1097

issn

1574-6968

journal_volume

57

pub_type

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