Identification and characterization of GroEL and DnaK homologues in Thiobacillus ferrooxidans.

abstract：:DNA uptake in competent Streptococcus pneumoniae was strongly dependent on intracellular pH. Ionophore treatments that either acidified or alkalinized the cytoplasm reduced DNA transport. This indicates that the optimum pH for DNA uptake corresponds to the intracellular pH of competent bacteria which is 8.3 +/- 0.2. I...

journal_title：FEMS microbiology letters

authors： Clavé C,Trombe MC

更新日期：1989-11-01 00:00:00

abstract：:Mixed-substrate conversions are an under-regarded option to fix carbon dioxide in significant amounts. In such a conversion, carbon dioxide together with one other carbon source such as glucose is converted to a single carbon product. With mixed-substrate conversions, it is possible to incorporate carbon dioxide into ...

journal_title：FEMS microbiology letters

authors： Steiger MG,Mattanovich D,Sauer M

更新日期：2017-11-15 00:00:00

abstract：:Paracoccus pantotrophus grown anaerobically under denitrifying conditions expressed similar levels of the periplasmic nitrate reductase (NAP) when cultured in molybdate- or tungstate-containing media. A native PAGE gel stained for nitrate reductase activity revealed that only NapA from molybdate-grown cells displayed ...

journal_title：FEMS microbiology letters

authors： Gates AJ,Hughes RO,Sharp SR,Millington PD,Nilavongse A,Cole JA,Leach ER,Jepson B,Richardson DJ,Butler CS

更新日期：2003-03-28 00:00:00

abstract：:An inducible mycelial beta-glucosidase from Scytalidum thermophilum was characterized. The enzyme exhibited a pI of 6.5, a carbohydrate content of 15%, and an apparent molecular mass of about 40 kDa. Optima of temperature and pH were 60 degrees C and 6.5, respectively. The enzyme was stable up to 1 h at 50 degrees C a...

journal_title：FEMS microbiology letters

authors： Zanoelo FF,Polizeli Mde L,Terenzi HF,Jorge JA

更新日期：2004-11-15 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) is a key enzyme in cellular one-carbon pathway and has been studied in many living organisms from bacteria to higher plants and mammals. However, biochemical and molecular characterization of SHMT from photoautotrophic microorganisms remains a challenge. Here, we isolated the SHM...

journal_title：FEMS microbiology letters

authors： Waditee-Sirisattha R,Sittipol D,Tanaka Y,Takabe T

更新日期：2012-08-01 00:00:00

abstract：:Two type B15 P1.16 strains of Neisseria meningitidis were examined by immunogold electron microscopy for accessibility of two outer membrane protein (OMPs) to monoclonal antibodies. Both strains exhibited cell-to-cell variation of one epitope of the Class 3 OMP (P3.15) and one of the Class 1 OMPs (P1.16) when grown in...

journal_title：FEMS microbiology letters

authors： Wall RA,Davies HA,Borriello SP

更新日期：1989-11-01 00:00:00

abstract：:The high-pathogenicity island (Yen HPI) of Yersinia enterocolitica biogroup (BG) 1B strains is associated with mouse virulence. Three repeated sequences are clustered on the A+T-rich part of the Yen HPI downstream of the fyuA yersiniabactin receptor gene in Y. enterocolitica O:8 strains WA-314 and 8081. In addition to...

journal_title：FEMS microbiology letters

authors： Rakin A,Schubert S,Guilvout I,Carniel E,Heesemann J

更新日期：2000-01-15 00:00:00

abstract：:A red-pigmented, Gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (TDMA-17(T)) was isolated from fresh water collected at Misasa, a radioactive site in Japan. TDMA-17(T) was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g(-1) dry c...

journal_title：FEMS microbiology letters

authors： Asker D,Beppu T,Ueda K

更新日期：2007-08-01 00:00:00

abstract：:Serratia entomophila and Serratia proteamaculans cause amber disease of the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). Three genes required for virulence, sepABC, are located on a large plasmid, pADAP. The translated products of the sep genes are members of the toxin complex (Tc) family of insecticid...

journal_title：FEMS microbiology letters

authors： Hurst MR,Jones SM,Tan B,Jackson TA

更新日期：2007-10-01 00:00:00

abstract：:In the purple sulphur bacterium Allochromatium vinosum, the prosthetic group of dissimilatory sulphite reductase (DsrAB) was identified as siroamide, an amidated form of the classical sirohaem. The genes dsrAB are the first two of a large cluster of genes necessary for the oxidation of sulphur globules stored intracel...

journal_title：FEMS microbiology letters

authors： Lübbe YJ,Youn HS,Timkovich R,Dahl C

更新日期：2006-08-01 00:00:00

abstract：:Insertion sequences (ISs) are mobile genetic elements widely distributed among bacteria. Their impact on the bacterial genome is multifold, including transfer of genetic information, shuttle of adaptive traits and influence on the genomic content. As a result, ISs play an important role in the organization, plasticity...

journal_title：FEMS microbiology letters

authors： Lysnyansky I,Calcutt MJ,Ben-Barak I,Ron Y,Levisohn S,Methé BA,Yogev D

更新日期：2009-05-01 00:00:00

abstract：:The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.I...

journal_title：FEMS microbiology letters

authors： Liu M,Zhao Y,Sun Y,Wu P,Zhou S,Ren L

更新日期：2020-09-01 00:00:00

abstract：:Fifty-five geographically different strains of Fusarium poae were assayed for the presence of extrachromosomal nucleic acid elements. All strains were found to harbour double-stranded RNA (dsRNA) elements and encapsidated virus-like particles (VLP). There were great individual differences in dsRNA patterns of the vari...

journal_title：FEMS microbiology letters

authors： Fekete C,Giczey G,Papp I,Szabó L,Hornok L

更新日期：1995-09-15 00:00:00

abstract：:We have developed a novel type of a positive screen for the discovery of antibacterial compounds that target the Escherichia coli replication initiator protein DnaA. DnaA is an essential replication protein, conserved in (almost) all bacteria--including all human pathogens--and no existing antibiotics target the main ...

journal_title：FEMS microbiology letters

authors： Fossum S,De Pascale G,Weigel C,Messer W,Donadio S,Skarstad K

更新日期：2008-04-01 00:00:00

abstract：:The transport of radioactively labelled uracil into submerged mycelium of T. viride was measured by means of a membrane filtration technique. It was found to be time-dependent (up to 90 min) and concentration-dependent (up to 8 mmol l-1). Its concentration dependence was biphasic and consisted from the saturatable par...

journal_title：FEMS microbiology letters

authors： Lakatos B,Simkovic M,Betina V,Varecka L

更新日期：1999-02-15 00:00:00

abstract：:A physical and functional map of Aspergillus tubingensis mtDNA type 2b was constructed and compared to Aspergillus niger typeI a mtDNA. The gene content and order, as well as the patterns of restriction sites, were similar. Two unidentified ORFs and several repeat elements were found in the region between the ndhl and...

journal_title：FEMS microbiology letters

authors： Juhász A,Láday M,Gácser A,Kucsera J,Pfeiffer I,Kevei F,Hamari Z

更新日期：2004-12-01 00:00:00

abstract：:Bacterial pathogens face constant challenges from DNA-damaging agents generated by host phagocytes. Although Borrelia burgdorferi appears to have much fewer DNA repair enzymes than pathogens with larger genomes, it does contain homologues of uvrA and uvrB (subunits A and B of excinuclease ABC). As a first step to expl...

journal_title：FEMS microbiology letters

authors： Sambir M,Ivanova LB,Bryksin AV,Godfrey HP,Cabello FC

更新日期：2011-04-01 00:00:00

abstract：:Porphyromonas gingivalis produces a trypsin-like enzyme, Protease I, which is thought to be an important virulence determinant of the organism in adult periodontal disease. Protease I is transiently inhibited by physiological inhibitors of human thrombin. The aim of the present work was to establish whether Protease I...

journal_title：FEMS microbiology letters

authors： Curtis MA,Macey M,Slaney JM,Howells GL

更新日期：1993-06-15 00:00:00

abstract：:We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera a...

journal_title：FEMS microbiology letters

authors： Cocolin L,Bisson LF,Mills DA

更新日期：2000-08-01 00:00:00

abstract：:There are two major pathways for methionine biosynthesis: the enterobacterial type transsulfuration pathway and the sulfhydrylation pathway as previously identified in the spirochete Leptospira meyeri. Sequence analysis of the L. meyeri metYX locus allows the identification of a third gene, called metW, which encodes ...

journal_title：FEMS microbiology letters

authors： Picardeau M,Bauby H,Saint Girons I

更新日期：2003-08-29 00:00:00

abstract：:A characteristic feature of fungal hypha is the presence of large number of nuclei in a common cytoplasmic environment. Where it has been examined, the coenocytic mycelium is commonly heterokaryotic. The nuclei cooperate, compete or combat. It is proposed that in addition to their classical role in heredity, supernume...

journal_title：FEMS microbiology letters

authors： Maheshwari R

更新日期：2005-08-01 00:00:00

abstract：:We have investigated the mechanisms used by Trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. Haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mM EGTA. Osmotic protection experiments using carbohydrates with different molecular diameters a...

journal_title：FEMS microbiology letters

authors： Fiori PL,Rappelli P,Rocchigiani AM,Cappuccinelli P

更新日期：1993-05-01 00:00:00

abstract：:Spores of Bacillus species have novel properties, which allow them to lie dormant for years and then germinate under favourable conditions. In the current work, the role of a key metabolic integrator, coenzyme A (CoA), in redox regulation of growing cells and during spore formation in Bacillus megaterium and Bacillus ...

journal_title：FEMS microbiology letters

authors： Zhyvoloup A,Yu BYK,Baković J,Davis-Lunn M,Tossounian MA,Thomas N,Tsuchiya Y,Peak-Chew SY,Wigneshweraraj S,Filonenko V,Skehel M,Setlow P,Gout I

更新日期：2020-12-22 00:00:00

abstract：:The presence and genetic content of integrons was investigated in eight Salmonella enteritica Typhimurium DT104 isolates from different pig herds in Denmark. Two different integrons were identified using PCR and sequencing. Each of the integrons carried a single resistance cassette in addition to the sull and qacE del...

journal_title：FEMS microbiology letters

authors： Sandvang D,Aarestrup FM,Jensen LB

更新日期：1998-03-01 00:00:00

abstract：:When challenged by stresses such as starvation, the soil bacterium Bacillus subtilis produces an endospore surrounded by a proteinaceous coat composed of >70 proteins that are organized into three main layers: an amorphous undercoat, lightly staining lamellar inner coat and electron-dense outer coat. This coat protect...

journal_title：FEMS microbiology letters

authors： Müllerová D,Krajčíková D,Barák I

更新日期：2009-10-01 00:00:00

abstract：:A chitinase was purified from the cytosolic fraction of the anaerobic rumen fungus Piromyces communis OTS1 by affinity chromatography using regenerated chitin, gel filtration and chromatofocusing. The chitinase was most active at pH 6.2 and at 60 degrees C in a 20-min assay. The molecular mass of the purified protein ...

journal_title：FEMS microbiology letters

authors： Sakurada M,Morgavi DP,Komatani K,Tomita Y,Onodera R

更新日期：1996-03-15 00:00:00

abstract：:Recent molecular approaches for the study of microbial communities such as PCR-cloning have enabled the detection and identification of as-yet-unculturable taxa. Cloning and sequencing of multiple samples is extremely laborious and expensive to perform thoroughly due to the large diversity involved. For this purpose, ...

journal_title：FEMS microbiology letters

authors： Gafan GP,Spratt DA

更新日期：2005-12-15 00:00:00

abstract：:The Staphylococcus aureus bacteriophage phi11 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and an SH3b cell wall-binding domain. In turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis pathogens, Staphylococcus aureus and coagulase-negative staphy...

journal_title：FEMS microbiology letters

authors： Donovan DM,Lardeo M,Foster-Frey J

更新日期：2006-12-01 00:00:00

abstract：:In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely unt...

journal_title：FEMS microbiology letters

authors： Berini F,Casciello C,Marcone GL,Marinelli F

更新日期：2017-11-15 00:00:00

abstract：:Comparison of sequence data is necessary in older to investigate virus origins, identify features common to virulent strains, and characterize genomic organization within virus families. A virulent caliciviral disease of rabbits recently emerged in China. We have sequenced 1100 bases from the 3' ends of two independen...

journal_title：FEMS microbiology letters

authors： Milton ID,Vlasak R,Nowotny N,Rodak L,Carter MJ

更新日期：1992-05-15 00:00:00