Abstract:
:To study the pathogenicity of mouse cytomegalovirus (MCMV) and to identify virulence determinants, we have isolated and phenotypically characterised 25 temperature-sensitive (ts) mutants. Six of these (tsm9, tsm13, tsm20, tsm22, tsm28 and tsm30) failed to replicate in mice and were avirulent. Five mutants (tsm14, tsm18, tsm19, tsm25 and tsm27) were of similar virulence to the parental wild-type (wt) virus, five (tsm7, tsm15, tsm24, tsm26 and tsm31) were 12-100 fold less virulent, five (tsm8, tsm12, tsm16, tsm23 and tsm29) were 150-1500 fold less virulent and four (tsm10, tsm11, tsm17 and tsm21) were between 2,000 and 85,000 fold less virulent than wt. One mutant (tsm28) did not plaque or replicate at 39 degrees C while 5 other mutants (tsm7, tsm9, tsm23, tsm24 and tsm27) also failed to plaque at 39 degrees C but only failed to replicate or replicated poorly at 40 degrees C. A further two mutants (tsm10 and tsm13) were able to plaque and replicate at 39 degrees C but not 40 degrees C. Six other mutants (tsm14, tsm15, tsm16, tsm21, tsm22 and tsm30) failed to form plaques at 40 degrees C and were severely restricted in their replication at 40 degrees C. The remaining 11 mutants exhibited varying degrees of restriction in ability to plaque and/or replicate at non-permissive temperatures. These 25 mutants, together with 6 isolated previously, comprise at least 24 complementation groups.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Akel HM,Sweet Cdoi
10.1111/j.1574-6968.1993.tb06523.xsubject
Has Abstractpub_date
1993-11-01 00:00:00pages
253-60issue
3eissn
0378-1097issn
1574-6968journal_volume
113pub_type
杂志文章abstract::In Bacillus subtilis, the yoxA and dacC genes were proposed to form an operon. The yoxA gene was overexpressed in Escherichia coli and its product fused to a polyhistidine tag was purified. An aldose-1-epimerase or mutarotase activity was measured with the YoxA protein that we propose to rename as GalM by analogy with...
journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1999.tb13592.x
更新日期:1999-05-15 00:00:00
abstract::The fungal inhibitory effects of strain Lactobacillus plantarum MiLAB 393, producing broad-spectrum antifungal compounds, were evaluated. A co-cultivation method was set up to monitor effects on fungal growth and protein expression of growing Aspergillus nidulans with L. plantarum MiLAB 393. The effects of inhibitory ...
journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.2002.tb11257.x
更新日期:2002-06-18 00:00:00
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2000.tb09054.x
更新日期:2000-04-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1994.tb06683.x
更新日期:1994-02-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/1574-6968.12319
更新日期:2014-01-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1999.tb13518.x
更新日期:1999-04-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnx151
更新日期:2017-09-01 00:00:00