Abstract:
:We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi strain Ty2. The gene showed a high degree of similarity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rfaH mutant was constructed by site-directed mutagenesis. This mutant produced a rough lipopolysaccharide (LPS), with an incomplete core region. The defect in LPS expression that results from the rfaH mutation was corrected by a plasmid carrying the intact gene. The plasmid-borne rfaH gene also restored normal LPS synthesis in a rfaH mutant of E. coli. Reverse transcription-polymerase chain reaction analyses were performed to determine the effects of various environmental conditions on the expression of rfaH. The transcription of rfaH showed a growth-phase-dependent regulation, with maximal expression at the late exponential phase. Other environmental conditions, such as temperature or medium osmolarity, did not affect transcription of rfaH.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Rojas G,Saldías S,Bittner M,Zaldívar M,Contreras Idoi
10.1111/j.1574-6968.2001.tb10874.xkeywords:
subject
Has Abstractpub_date
2001-10-16 00:00:00pages
123-8issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(01)00390-1journal_volume
204pub_type
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