Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis.

abstract：:Ammonium transporters form a conserved family of transport proteins and are widely distributed among all domains of life. The genome of Nitrosomonas europaea codes for a single gene (rh1) that belongs to the family of the AMT/Rh ammonium transporters. For the first time, this study provides functional and physiologica...

journal_title：FEMS microbiology letters

authors： Weidinger K,Neuhäuser B,Gilch S,Ludewig U,Meyer O,Schmidt I

更新日期：2007-08-01 00:00:00

abstract：:High resistance to class IIa bacteriocins in Listeria monocytogenes has been clearly linked to lack of expression of the mptACD operon, encoding the EIIt Man mannose PTS permease. Also, intermediate resistance has been associated with membrane phospholipid modifications in the spontaneous mutants L. monocytogenes B73-...

journal_title：FEMS microbiology letters

authors： Arous S,Dalet K,Héchard Y

更新日期：2004-09-01 00:00:00

abstract：:Trichoderma reesei is a filamentous fungus widely used as an efficient protein producer and known to secrete large quantities of biomass degrading enzymes. Much work has been done aimed at improving the secretion efficiency of this fungus. It is generally accepted that the major bottlenecks in secretion are protein fo...

journal_title：FEMS microbiology letters

authors： Diener SE,Dunn-Coleman N,Foreman P,Houfek TD,Teunissen PJ,van Solingen P,Dankmeyer L,Mitchell TK,Ward M,Dean RA

更新日期：2004-01-30 00:00:00

abstract：:MHYT, a new conserved protein domain with a likely signaling function, is described. This domain consists of six transmembrane segments, three of which contain conserved methionine, histidine, and tyrosine residues that are projected to lie near the outer face of the cytoplasmic membrane. In Synechocystis sp. PCC6803,...

journal_title：FEMS microbiology letters

authors： Galperin MY,Gaidenko TA,Mulkidjanian AY,Nakano M,Price CW

更新日期：2001-11-27 00:00:00

abstract：:The RpoS sigma factor is required for the transition of Escherichia coli into stationary phase, as well as adaptation to environmental stresses and nutrient depletion. In this study, we report that under nutrient poor conditions, RpoS protein accumulation in E. coli was strongly enhanced by a secreted factor. Expressi...

journal_title：FEMS microbiology letters

authors： Holland AM,Rather PN

更新日期：2008-09-01 00:00:00

abstract：:Dimethylphthalate (DMP), one of the phthalate esters, is used in the manufacture of plasticizers, insect repellents, and synthetic fibers, and contributes to environmental pollution. In the present study, we report a novel bacterium belonging to the Bacillus sp., which has the ability to utilize DMP as the sole source...

journal_title：FEMS microbiology letters

authors： Niazi JH,Prasad DT,Karegoudar TB

更新日期：2001-03-15 00:00:00

abstract：:The effectiveness of an educational board game developed to teach the pharmacology of antimicrobial drugs to medical students was compared with the lecture-based seminar as a supplemental tool to improve short- and long-term knowledge retention and the perception of the learning method by students. A group of 124 stud...

journal_title：FEMS microbiology letters

authors： Karbownik MS,Wiktorowska-Owczarek A,Kowalczyk E,Kwarta P,Mokros Ł,Pietras T

更新日期：2016-04-01 00:00:00

abstract：:The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from t...

journal_title：FEMS microbiology letters

authors： Brans A,Loriaux A,Thamm I,Joris B,Dusart J

更新日期：1997-04-15 00:00:00

abstract：:The distribution of the alcohol dehydrogenase gene (adh) among different Archaea was investigated by Southern blot analysis revealing the potentiality of the adh gene as a specific marker for the genus Sulfolobus. Moreover, the in vivo expression of the adh gene from a new isolate of Sulfolobus solfataricus, G theta, ...

journal_title：FEMS microbiology letters

authors： Cannio R,Fiorentino G,Rossi M,Bartolucci S

更新日期：1999-01-01 00:00:00

abstract：:This report describes Vibrio seventh pandemic island II (VSP-II) and three novel variants revealed by comparative genomics of 23 Vibrio cholerae strains and their presence among a large and diverse collection of V. cholerae isolates. Three VSP-II variants were reported previously and our results demonstrate the presen...

journal_title：FEMS microbiology letters

authors： Taviani E,Grim CJ,Choi J,Chun J,Haley B,Hasan NA,Huq A,Colwell RR

更新日期：2010-07-01 00:00:00

abstract：:To determine the existence of an acid stress response in Helicobacter pylori the global changes in the proteins synthesized by the bacterium when subjected to an acid stress were studied. H. pylori ATCC43504 previously adapted to pH 7 did not show an acid stress response as detected by the two-dimensional electrophore...

journal_title：FEMS microbiology letters

authors： Toledo H,Valenzuela M,Rivas A,Jerez CA

更新日期：2002-07-16 00:00:00

abstract：:Methanotrophic populations have been studied in Newport Bay estuary, Southern California. Environmental clone banks were generated for 16S rRNA genes specific to methanotrophs and for a diagnostic functional gene, pmoA, encoding a conserved subunit of the particulate methane monooxygenase. These clone banks contained ...

journal_title：FEMS microbiology letters

authors： McDonald IR,Smith K,Lidstrom ME

更新日期：2005-09-15 00:00:00

abstract：:Atypical psychrophilic Aeromonas salmonicida isolates were obtained from farmed and wild fish in Northeastern North America. These bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (Tc(r)) plasmids of approximately 58 kb. The nine isolates had plasmids which could be divided into four gr...

journal_title：FEMS microbiology letters

authors： Casas C,Anderson EC,Ojo KK,Keith I,Whelan D,Rainnie D,Roberts MC

更新日期：2005-01-01 00:00:00

abstract：:Expression of the dimethylsulfoxide respiratory (dor) operon of Rhodobacter is regulated by oxygen, light intensity and availability of substrate. Since dimethylsulfoxide reductase contains a pterin molybdenum cofactor, the role of molybdate in the regulation of dor operon expression was investigated. In this report w...

journal_title：FEMS microbiology letters

authors： Solomon PS,Shaw AL,Young MD,Leimkuhler S,Hanson GR,Klipp W,McEwan AG

更新日期：2000-09-15 00:00:00

abstract：:During bacterial infection, professional phagocytes are attracted to the site of infection, where they constitute a first line of host cell defense. Their function is to engulf and destroy the pathogens. Thus, bacteria must withstand the bactericidal activity of professional phagocytes, including macrophages to counte...

journal_title：FEMS microbiology letters

authors： Tran SL,Ramarao N

更新日期：2013-10-01 00:00:00

abstract：:Mycoplasma fermentans has been reported to be pathogenic for man. All fourteen strains tested contain an insertion sequence-like element (ISLE) which may be present in multiple copies. To determine whether ISLE copies are similarly distributed in different strains of M. fermentans, restriction enzyme digest fragments ...

journal_title：FEMS microbiology letters

authors： Pitcher D,Hilbocus J

更新日期：1998-03-01 00:00:00

abstract：:A family of stable lactococcal vectors have been constructed based on the pFX1 replicon using either the alpha fragment or the complete Escherichia coli lacZ gene as a selective marker. These vectors also incorporate multiple cloning sites and examples are given of their use for gene cloning and translational fusion s...

journal_title：FEMS microbiology letters

authors： Xu FF,Pearce LE,Yu PL

更新日期：1991-01-01 00:00:00

abstract：:In the current studies, we used Lineweaver-Burke analysis to examine the role of 1-hydroxybenzotriazole (HBT) in the oxidation of various compounds by laccase from Trametes versicolor. At low concentrations, HBT was a competitive inhibitor of the oxidation, but at high concentrations, it was a noncompetitive inhibitor...

journal_title：FEMS microbiology letters

authors： Hirai H,Shibata H,Kawai S,Nishida T

更新日期：2006-12-01 00:00:00

abstract：:Five transposon mutants of Francisella novicida were isolated that are compromised in their ability to grow in mouse macrophages in vitro. Sequence analysis of the DNA flanking the transposon insertions identified the genes that were interrupted in these mutants. One of the inactivated loci corresponds to the Francise...

journal_title：FEMS microbiology letters

authors： Gray CG,Cowley SC,Cheung KK,Nano FE

更新日期：2002-09-24 00:00:00

abstract：:The capsular polysaccharide released from the bacterial surface by cell wall turnover during growth exhibited less size heterogeneity and a higher average molecular mass than the polysaccharide extracted from the cell by treatment with lysostaphin or low pH. Treatment of turnover polysaccharide, radiolabelled by growt...

journal_title：FEMS microbiology letters

authors： Havaei SA,Hancock IC

更新日期：1994-05-01 00:00:00

abstract：:The anr gene of Pseudomonas aeruginosa encodes a transcriptional regulator of anaerobic gene expression, homologous to the Fnr protein of Escherichia coli. We report here that Anr has a role in regulating the activity of the aerobic respiratory chain of P. aeruginosa. Strains with internal deletions in their anr gene ...

journal_title：FEMS microbiology letters

authors： Ray A,Williams HD

更新日期：1997-11-15 00:00:00

abstract：:Two cDNAs encoding feedback inhibition-insensitive serine acetyltransferases of Arabidopsis thaliana were expressed in the chromosomal serine acetyltransferase-deficient and L-cysteine non-utilizing Escherichia coli strain JM39-8. The transformants produced 1600 to 1700 mg l(-1) of L-cysteine and L-cystine from glucos...

journal_title：FEMS microbiology letters

authors： Takagi H,Awano N,Kobayashi S,Noji M,Saito K,Nakamori S

更新日期：1999-10-15 00:00:00

abstract：:Intracellular proteases of Yarrowia lipolytica have been scarcely studied. These enzymes may play an important role in nitrogen metabolism, posttranslational processing, nutritional stress, dimorphism, etc.; biochemical and genetic control of these enzymes can help in obtaining high-level expression of recombinant pro...

journal_title：FEMS microbiology letters

authors： Hernández-Montañez Z,Araujo-Osorio J,Noriega-Reyes Y,Chávez-Camarillo G,Villa-Tanaca L

更新日期：2007-03-01 00:00:00

abstract：:A continuous culture technique utilising a variant of Staphylococcus aureus 8325-4 containing transcriptional gene fusions was used to investigate the relationships between cell density (OD(600)), steady-state specific growth rate (mu) and expression of both agr (accessory gene regulator) and tst (toxic shock syndrome...

journal_title：FEMS microbiology letters

authors： Wright JD,Holland KT

更新日期：2003-01-28 00:00:00

abstract：:Mycoplasma salivarium cells bound the Fc fragment of human immunoglobulin G. The activity was remarkably enhanced by Mn2+, but not by Mg2+ and Ca2+; significantly inhibited by D-mannose; and reduced by pronase treatment of the cells. About 90% of the cells treated with pronase were not disrupted. Sodium dodecyl sulfat...

journal_title：FEMS microbiology letters

authors： Shibata K,Sawa Y,Inoue S,Noda M,Watanabe T

更新日期：1994-11-01 00:00:00

abstract：:Conditional gene expression systems are useful tools for studying the role of essential or toxic gene products in bacterial systems. There is a paucity of such systems available for use in the mycobacteria. The utility of the Escherichia coli arabinose-inducible system was looked into, since it is tightly controlled i...

journal_title：FEMS microbiology letters

authors： Carroll P,Brown AC,Hartridge AR,Parish T

更新日期：2007-09-01 00:00:00

abstract：:A plasmid-based food-grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MG5267 is a plasmid-free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross-over homologous recombina...

journal_title：FEMS microbiology letters

authors： MacCormick CA,Griffin HG,Gasson MJ

更新日期：1995-03-15 00:00:00

abstract：:Twelve strains of Candida albicans were grown in defined medium which had been deferrated by ion-exchange chromatography and then supplemented with FeCl3 to give iron concentrations ranging from 0.026 microM (growth-limiting) to 0.8 microM (excess). All of the strains secreted hydroxamate-type siderophores; phenolate ...

journal_title：FEMS microbiology letters

authors： Sweet SP,Douglas LJ

更新日期：1991-05-01 00:00:00

abstract：:DNA hybridization using the Synechocystis PCC6803 glucose transporter gene, glcP, revealed a single homologous region in two facultative photoautotrophic strains out of three tested, and none in three obligate autotrophs. In one of the latter, Synechococcus PCC7942, integration of glcP into the chromosome resulted in ...

journal_title：FEMS microbiology letters

authors： Zhang CC,Jeanjean R,Joset F

更新日期：1998-04-15 00:00:00

abstract：:Recent molecular approaches for the study of microbial communities such as PCR-cloning have enabled the detection and identification of as-yet-unculturable taxa. Cloning and sequencing of multiple samples is extremely laborious and expensive to perform thoroughly due to the large diversity involved. For this purpose, ...

journal_title：FEMS microbiology letters

authors： Gafan GP,Spratt DA

更新日期：2005-12-15 00:00:00