Pyrimidine scavenging by Mycobacterium leprae.

Abstract:

:Mycobacterium leprae incorporated exogenously supplied pyrimidines as bases or nucleosides, but not as a nucleotide, into its nucleic acids. Notably, thymine was incorporated approximately 5 times more rapidly than thymidine by both suspensions of, or intracellular M. leprae. Thymine incorporation was significantly inhibited by clofazamine and dapsone at near-pharmacological levels. Therefore, incorporation of thymine is preferable as an activity for assessing viability of M. leprae. Nucleosides were converted to nucleotides through kinases, bases through phosphoribosyltransferases. Alternatively, thymine and uracil could first be converted to nucleosides. Cytosine and uracil bases were interconvertible, and uracil alone could supply all the pyrimidine requirements of M. leprae, though conversion to the thymine base was extremely slow. Overall, pyrimidine scavenging occurs at a slower rate than, and appears not to be so important as purine scavenging in M. leprae.

journal_name

FEMS Microbiol Lett

authors

Wheeler PR

doi

10.1111/j.1574-6968.1989.tb03295.x

subject

Has Abstract

pub_date

1989-01-15 00:00:00

pages

179-84

issue

2

eissn

0378-1097

issn

1574-6968

journal_volume

48

pub_type

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