ERIC- and REP-PCR amplify non-repetitive fragments from the genome of Drechslera avenae and Stemphylium solani.

Abstract:

:In the present study, the homology relationships among fragments amplified by ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Extragenic Palindromic) primers in Drechslera avenae and Stemphylium solani causing leaf blotch and black spot of oats and leaf blight of cotton, respectively, were investigated by Southern hybridization followed by DNA sequencing. ERIC- and REP-PCR were performed for 24 isolates of D. avenae and 33 isolates of S. solani. The banding patterns obtained were transferred to nylon membranes and a total of 16 fragments, four ERIC and four REP bands from each fungus, used as probes. The hybridization and DNA sequencing revealed a low degree of similarity among the bands indicating that the regions amplified by these primers are not repetitive and that the primers anneal at random regions in the genomes of D. avenae and S. solani.

journal_name

FEMS Microbiol Lett

authors

Mehta A,Mehta YR,Rosato YB

doi

10.1111/j.1574-6968.2002.tb11202.x

keywords:

subject

Has Abstract

pub_date

2002-05-21 00:00:00

pages

51-5

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378109702006432

journal_volume

211

pub_type

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