ERIC- and REP-PCR amplify non-repetitive fragments from the genome of Drechslera avenae and Stemphylium solani.


:In the present study, the homology relationships among fragments amplified by ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Extragenic Palindromic) primers in Drechslera avenae and Stemphylium solani causing leaf blotch and black spot of oats and leaf blight of cotton, respectively, were investigated by Southern hybridization followed by DNA sequencing. ERIC- and REP-PCR were performed for 24 isolates of D. avenae and 33 isolates of S. solani. The banding patterns obtained were transferred to nylon membranes and a total of 16 fragments, four ERIC and four REP bands from each fungus, used as probes. The hybridization and DNA sequencing revealed a low degree of similarity among the bands indicating that the regions amplified by these primers are not repetitive and that the primers anneal at random regions in the genomes of D. avenae and S. solani.


FEMS Microbiol Lett


Mehta A,Mehta YR,Rosato YB





Has Abstract


2002-05-21 00:00:00














  • Bacterial degradation of natural rubber: a privilege of actinomycetes?

    abstract::Using natural rubber latex as the sole source of carbon and energy 50 rubber-degrading bacteria were isolated. Out of those 50 isolates, 33 were identified as Streptomyces species and 8 as Micromonospora species. Screening of 1220 bacteria obtained from different culture collections revealed 46 additional rubber-degra...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jendrossek D,Tomasi G,Kroppenstedt RM

    更新日期:1997-05-15 00:00:00

  • CIA2 deficiency results in impaired oxidative stress response and enhanced intracellular basal UPR activity in Saccharomyces cerevisiae.

    abstract::Yeast Cia2p is a component of the cytosolic Fe/S protein assembly (CIA) machinery. Initial studies of the CIA machinery were performed in yeast, but the precise role of Cia2p in this eukaryote is still unknown. We report that CIA2 deficiency results in impaired oxidative stress response, as evidenced by increased sens...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zhao W,Zheng HZ,Niu YJ,Yuan Y,Fang BX,Liu YN,Cai LH,Zhou ZJ,Liu XG

    更新日期:2015-03-01 00:00:00

  • Lactobacillus reuteri A9 and Lactobacillus mucosae A13 isolated from Chinese superlongevity people modulate lipid metabolism in a hypercholesterolemia rat model.

    abstract::While there is strong evidence showing that many food-borne probiotics regulate cholesterol metabolism, few studies have examined how probiotics of human origin affect cholesterol metabolism. Because people living in so-called 'longevity villages' are unlikely to have hypercholesterolemia, we hypothesized that probiot...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jiang J,Feng N,Zhang C,Liu F,Zhao J,Zhang H,Zhai Q,Chen W

    更新日期:2019-12-01 00:00:00

  • Structural studies on the Ebola virus matrix protein VP40 indicate that matrix proteins of enveloped RNA viruses are analogues but not homologues.

    abstract::Matrix proteins are the driving force of assembly of enveloped viruses. Their main function is to interact with and polymerize at cellular membranes and link other viral components to the matrix-membrane complex resulting in individual particle shapes and ensuring the integrity of the viral particle. Although matrix p...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Timmins J,Ruigrok RW,Weissenhorn W

    更新日期:2004-04-15 00:00:00

  • Isolation and characterization of bacteriophages infecting Salmonella spp.

    abstract::Bacteriophages infecting Salmonella spp. were isolated from sewage using soft agar overlays containing three Salmonella serovars and assessed with regard to their potential to control food-borne salmonellae. Two distinct phages, as defined by plaque morphology, structure and host range, were obtained from a single sam...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Carey-Smith GV,Billington C,Cornelius AJ,Hudson JA,Heinemann JA

    更新日期:2006-05-01 00:00:00

  • Relationships between phosphatidylcholine content, chitin synthesis, growth, and morphology of Aspergillus nidulans choC.

    abstract::The phosphatidylcholine (PC) content of Aspergillus nidulans choC was varied by growing the auxotroph in medium containing various concentrations of choline chloride. Direct linear correlations were observed between PC content and in vivo chitin synthase activity, between in vivo chitin synthase activity and mean hyph...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Binks PR,Robson GD,Goosey MW,Trinci AP

    更新日期:1991-10-01 00:00:00

  • Infection of human B lymphoma cells by Mycoplasma fermentans induces interaction of its elongation factor with the intracytoplasmic domain of Epstein-Barr virus receptor (gp140, EBV/C3dR, CR2, CD21).

    abstract::Human cell lines are often infected by mycoplama strains. We have demonstrated that when infected by Mycoplasma fermentans, human B lymphoma cell proliferation increased strongly. These infected B cells expressed a p45 kDa protein which interacted with the intracellular domain of CD21, the EBV/C3d receptor. p45 analys...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Balbo M,Barel M,Lottin-Divoux S,Jean D,Frade R

    更新日期:2005-08-15 00:00:00

  • Conserved motifs in the CRISPR leader sequence control spacer acquisition levels in Type I-D CRISPR-Cas systems.

    abstract::Integrating short DNA fragments at the correct leader-repeat junction is key to successful CRISPR-Cas memory formation. The Cas1-2 proteins are responsible to carry out this process. However, the CRISPR adaptation process additionally requires a DNA element adjacent to the CRISPR array, called leader, to facilitate ef...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kieper SN,Almendros C,Brouns SJJ

    更新日期:2019-06-01 00:00:00

  • Characterization of a Mycobacterium bovis BCG insertion sequence related to the IS21 family.

    abstract::The structure and distribution of a Mycobacterium bovis BCG insertion element of the IS21 family were investigated. Several IS21-like elements found in mycobacterial genomes were separated in four types, following their nucleic acid similarities. The M. bovis BCG IS21 element is highly similar to IS1533 (class I), 70%...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lefèvre P,Braibant M,Content J,Gilot P

    更新日期:1999-09-15 00:00:00

  • Identification of genes coding for hydrolytic dehalogenation in the metagenome derived from a denitrifying 4-chlorobenzoate degrading consortium.

    abstract::A metagenomic approach was taken to investigate the genetic basis for the ability of an anaerobic consortium to grow on either 4-chlorobenzoate or 4-bromobenzoate under denitrifying conditions. Degenerate PCR primers were designed for the family of 4-chlorobenzoyl-CoA dehalogenase genes. The primers were utilized to s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chae JC,Song B,Zylstra GJ

    更新日期:2008-04-01 00:00:00

  • The LIV-I/LS system as a determinant of azaserine sensitivity of Escherichia coli K-12.

    abstract::The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidat...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Koyanagi T,Katayama T,Suzuki H,Kumagai H

    更新日期:2004-08-01 00:00:00

  • NtrBC-dependent expression from the Rhizobium meliloti dctA promoter in Escherichia coli.

    abstract::Effects of the two-component sensor-regulator pairs DctBD and NtrBC upon the expression of a dctA::phoA fusion from Rhizobium meliloti were determined under excess and limiting nitrogen concentrations in Escherichia coli. Results indicated that NtrBC affected transcription from the dctA promoter on a number of regulat...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Allaway D,Boesten B,O'Gara F

    更新日期:1995-05-15 00:00:00

  • Expression of the landomycin biosynthetic gene cluster in a PKS mutant of Streptomyces fradiae is dependent on the coexpression of a putative transcriptional activator gene.

    abstract::The formation of landomycin A or one of its derivatives (5,6-anhydrolandomycin A) in a heterologous strain has never been achieved. It has now been made possible by the coexpression of a cosmid containing all biosynthetic genes necessary to produce landomycin A together with a pathway-specific regulatory gene. As host...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: von Mulert U,Luzhetskyy A,Hofmann C,Mayer A,Bechthold A

    更新日期:2004-01-15 00:00:00

  • OK, thanks! A new mutualism between Chlamydomonas and methylobacteria facilitates growth on amino acids and peptides.

    abstract::Nitrogen is a key nutrient for land plants and phytoplankton in terrestrial and aquatic ecosystems. The model alga Chlamydomonas reinhardtii can grow efficiently on several inorganic nitrogen sources (e.g. ammonium, nitrate, nitrite) as well as many amino acids. In this study, we show that Chlamydomonas is unable to u...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Calatrava V,Hom EFY,Llamas Á,Fernández E,Galván A

    更新日期:2018-04-01 00:00:00

  • Cellulase and hemicellulase genes of Clostridium thermocellum from five independent collections contain few overlaps and are widely scattered across the chromosome.

    abstract::Five independent collections, comprising a total of 34 clones encoding cellulases, hemicellulases and cell surface proteins of Clostridium thermocellum, were searched for overlapping or contiguous DNA fragments. The clones were hybridized to large genomic restriction fragments separated by pulse-field electrophoresis....

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Guglielmi G,Béguin P

    更新日期:1998-04-01 00:00:00

  • Effect of coumarin on glucose uptake by anaerobic rumen fungi in the presence and absence of Methanobrevibacter smithii.

    abstract::The effect of coumarin (1,2 benzopyrone) on glucose utilisation by the anaerobic rumen fungi Neocallimastix frontalis and N. patriciarum has been compared with the effect of p-coumaric acid. Both compounds largely inhibited glucose utilisation by N. patriciarum strain Cx when present in the medium at a concentration o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cansunar E,Richardson AJ,Wallace G,Stewart CS

    更新日期:1990-07-01 00:00:00

  • A peptidoglycan binding domain in the porin-associated protein (PAP) of Rhodospirillum rubrum FR1.

    abstract::The porin-associated protein of Rhodospirillum rubrum FR1 was found to contain a peptidoglycan binding motif. A partial fragment of 179 amino acids, obtained by cleavage of PAP with trypsin, Asp-N protease, and CNBr, was sequenced. Substantial sequence homology was found of the C-terminal part (residues 126-179) of po...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Neumann U,Schiltz E,Stahl B,Hillenkamp F,Weckesser J

    更新日期:1996-04-15 00:00:00

  • Cloning and analysis of the polyhydroxyalkanoic acid synthase gene from an Acinetobacter sp.: evidence that the gene is both plasmid and chromosomally located.

    abstract::The polyhydroxyalkanoic acid (PHA) synthase gene (phaCAc) of a species of Acinetobacter isolated from an activated sludge treatment plant was cloned by heterologous complementation in a poly-beta-hydroxybutyrate (PHB) negative mutant of Alcaligenes eutrophus. Nucleotide sequence analysis of phaCAc revealed an open rea...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Schembri MA,Bayly RC,Davies JK

    更新日期:1994-05-01 00:00:00

  • Hydrogenotrophic culture enrichment reveals rumen Lachnospiraceae and Ruminococcaceae acetogens and hydrogen-responsive Bacteroidetes from pasture-fed cattle.

    abstract::Molecular information suggests that there is a broad diversity of acetogens in the rumen, distinct from any currently isolated acetogens. We combined molecular analysis with enrichment culture techniques to investigate this diversity further. Methane-inhibited, hydrogenotrophic enrichment cultures produced acetate as ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gagen EJ,Padmanabha J,Denman SE,McSweeney CS

    更新日期:2015-07-01 00:00:00

  • Isolation and characterization of Hena1 - a novel Erwinia amylovora bacteriophage.

    abstract::Fire blight, caused by plant pathogenic bacterium Erwinia amylovora, is one of the most important diseases of Rosaceae plants. Due to the lack of effective control measures, fire blight infections pose a recurrent threat on agricultural production worldwide. Recently, bacterial viruses, or bacteriophages, have been pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Besarab NV,Akhremchuk AE,Zlatohurska MA,Romaniuk LV,Valentovich LN,Tovkach FI,Lagonenko AL,Evtushenkov AN

    更新日期:2020-05-01 00:00:00

  • Temperature-sensitive recombinant subtilisin protease variants that efficiently degrade molecular biology enzymes.

    abstract::We used error-prone PCR to generate mutations in a subtilisin protease-encoding gene, and screened for recombinants that expressed temperature-sensitive (TS) variants. From the dozens of mutations that we detected in the recombinant genes we found that those mutations that affected aspartate-75 had the most profound e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Thompson VC,McGuire BE,Frier MS,Legg MSG,Dyer TW,Gudavicius G,Potter S,Nano FE

    更新日期:2020-10-16 00:00:00

  • Regulatory systems controlling motility and gene transfer agent production and release in Rhodobacter capsulatus.

    abstract::Production of the gene transfer agent of Rhodobacter capsulatus, RcGTA, is dependent upon several cellular regulatory systems, including a putative phosphorelay involving the CtrA and CckA proteins. These proteins are also involved in flagellar motility in R. capsulatus. The interactions of proteins in this system are...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mercer RG,Quinlan M,Rose AR,Noll S,Beatty JT,Lang AS

    更新日期:2012-06-01 00:00:00

  • Measurement of Pseudomonas aeruginosa multidrug efflux pumps by quantitative real-time polymerase chain reaction.

    abstract::Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yoneda K,Chikumi H,Murata T,Gotoh N,Yamamoto H,Fujiwara H,Nishino T,Shimizu E

    更新日期:2005-02-01 00:00:00

  • Bacillus thuringiensis serovar israelensis is highly toxic to the coffee berry borer, Hypothenemus hampei Ferr. (Coleoptera: Scolytidae).

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Méndez-López I,Basurto-Ríos R,Ibarra JE

    更新日期:2003-09-12 00:00:00

  • Identification of immunogenic antigens of Helicobacter pylori via the Escherichia coli hemolysin secretion system(1).

    abstract::We describe a new procedure allowing the generation and detection of immunogenic antigens from Helicobacter pylori via the hemolysin secretion apparatus of Escherichia coli. The gene (or gene fragment) encoding the H. pylori protein (or protein domain) is inserted in-frame into a residual portion of the hemolysin gene...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Spreng S,Gentschev I,Goebel W,Mollenkopf H,Eck M,Müller-Hermelink HK,Schmausser B

    更新日期:2000-05-15 00:00:00

  • Non-radioactive restriction fragment length polymorphism (RFLP) typing of Clostridium difficile.

    abstract::A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemilumine...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bowman RA,O'Neill GL,Riley TV

    更新日期:1991-04-15 00:00:00

  • Adenylate kinase isoenzymes in Aspergillus nidulans.

    abstract::Adenylate kinase isoenzymes localised in the mitochondria and in the cytosol have been detected in extracts of glucose-grown Aspergillus nidulans using specific staining after electrophoresis on cellulose acetate. The isoenzymes have similar Km values for AMP, ADP and MgATP2- but may differ in the mechanism used for i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hoang VQ,Jaklitsch W,Scrutton MC

    更新日期:1991-03-01 00:00:00

  • Transcriptional regulation of pentose utilisation systems in the Bacillus/Clostridium group of bacteria.

    abstract::In Bacillus subtilis, utilisation of xylose, arabinose and ribose is controlled by the transcriptional factors XylR, AraR and RbsR, respectively. Here we apply the comparative approach to the analysis of these regulons in the Bacillus/Clostridium group. Evolutionary variability of operon structures is demonstrated and...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rodionov DA,Mironov AA,Gelfand MS

    更新日期:2001-12-18 00:00:00

  • Inactivation of the petE gene encoding plastocyanin causes different photosynthetic responses in cyanobacterium Synechocystis PCC 6803 under light-dark photoperiod and continuous light conditions.

    abstract::Plastocyanin, encoded by the petE gene, can transfer electrons to photosystem I (PSI) and cytochrome c oxidase during photosynthetic and respiratory metabolism in cyanobacteria. We constructed a petE mutant of Synechocystis sp. strain PCC 6803 and investigated its phenotypic properties under different light conditions...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Wang XQ,Jiang HB,Zhang R,Qiu BS

    更新日期:2013-04-01 00:00:00

  • Microorganisms form exocellular structures, trophosomes, to facilitate biodegradation of oil in aqueous media.

    abstract::Cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. Among the microorganisms studied here, the yeasts (Schwanniomyces occidentalis, Torulopsis candida, Candida tro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dmitriev VV,Crowley D,Rogachevsky VV,Negri CM,Rusakova TG,Kolesnikova SA,Akhmetov LI

    更新日期:2011-02-01 00:00:00