Abstract:
:In the present study, the homology relationships among fragments amplified by ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Extragenic Palindromic) primers in Drechslera avenae and Stemphylium solani causing leaf blotch and black spot of oats and leaf blight of cotton, respectively, were investigated by Southern hybridization followed by DNA sequencing. ERIC- and REP-PCR were performed for 24 isolates of D. avenae and 33 isolates of S. solani. The banding patterns obtained were transferred to nylon membranes and a total of 16 fragments, four ERIC and four REP bands from each fungus, used as probes. The hybridization and DNA sequencing revealed a low degree of similarity among the bands indicating that the regions amplified by these primers are not repetitive and that the primers anneal at random regions in the genomes of D. avenae and S. solani.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Mehta A,Mehta YR,Rosato YBdoi
10.1111/j.1574-6968.2002.tb11202.xkeywords:
subject
Has Abstractpub_date
2002-05-21 00:00:00pages
51-5issue
1eissn
0378-1097issn
1574-6968pii
S0378109702006432journal_volume
211pub_type
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