Abstract:
:Picrophilus torridus has been shown to degrade glucose via a nonphosphorylative Entner-Doudoroff (ED) pathway. Here we report the characterization of a key enzyme of this pathway, glycerate kinase (2-phosphoglycerate forming). The enzyme was purified 5,100-fold to homogeneity. The 95 kDa homodimeric protein catalyzed the ATP-dependent phosphorylation of glycerate specifically to 2-phosphoglycerate. The enzyme showed highest activity at 60 degrees C and pH 7.3, with ATP as phosphoryl donor and Mg(2+) as divalent cation. By MALDI-TOF analysis, ORF Pto1442 was identified in the genome of P. torridus as the encoding gene, designated gck. Homologs with high sequence identity were identified in the genomes of the archaea Thermoplasma and Sulfolobus spp. and Thermoproteus tenax, for which the operation of nonphosphorylative ED pathways, involving 2-phosphoglycerate forming glycerate kinases, has been proposed.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Reher M,Bott M,Schönheit Pdoi
10.1111/j.1574-6968.2006.00264.xsubject
Has Abstractpub_date
2006-06-01 00:00:00pages
113-9issue
1eissn
0378-1097issn
1574-6968pii
FML264journal_volume
259pub_type
杂志文章abstract::Based on second generation sequencing (MiSeq platform, Illumina), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obt...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnx264
更新日期:2018-02-01 00:00:00
abstract::The cellulase-producing fungal strain Y-94, isolated in Japan and invalidly described as Acremonium cellulolyticus nom. nud. strain Y-94, seldom forms enteroarthric conidia under nutrient starvation conditions. Phylogenetic analysis using ITS1-5.8S-ITS2 and RNA polymerase II large subunit gene sequences revealed that ...
journal_title:FEMS microbiology letters
pub_type: 信件
doi:10.1111/1574-6968.12352
更新日期:2014-02-01 00:00:00
abstract::The purpose of this study was to investigate the feasibility of cultivating the biotechnologically important bacterium Streptomyces griseus in single-species and mixed-species biofilms using a tubular biofilm reactor (TBR). Streptomyces griseus biofilm development was found to be cyclical, starting with the initial ad...
journal_title:FEMS microbiology letters
pub_type: 信件
doi:10.1111/1574-6968.12378
更新日期:2014-03-01 00:00:00
abstract::The influence of nutritional and physical stress on sporulation, conidial germination and vegetative biomass of Ophiocordyceps sinensis, one of the most important medicinal fungi in China and now globally, was evaluated using a two-stage culture method. All the treatments, except nutrient deprivation, enhanced conidia...
journal_title:FEMS microbiology letters
pub_type: 信件
doi:10.1111/1574-6968.12190
更新日期:2013-09-01 00:00:00
abstract::The quorum quenching (QQ) activity of endophytic bacteria associated with medicinal plants was explored. Extracts of the Gram-negative Enterobacter sp. CS66 possessed potent N-acylhomoserine lactone (AHL) hydrolytic activity in vitro. Using degenerate primers, we PCR-amplified an open reading frame (denoted aiiE) from...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fny054
更新日期:2018-05-01 00:00:00
abstract::Bacterial swarming motility is a flagella-dependent translocation on the surface environment. It has received extensive attention as a population behavior involving numerous genes. Here, we report that Citrobacter freundii, an opportunistic pathogen, exhibits swarming movement on a solid medium surface with appropriat...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2011.02225.x
更新日期:2011-04-01 00:00:00
abstract::The Mycobacterium tuberculosis murG gene, Rv2153, was expressed in Escherichia coli murG(Ts) strain OV58 on a plasmid under the control of the arabinose-inducible araBAD promoter. Mycobacterium tuberculosis murG rescued the growth of E. coli murG(Ts) at the nonpermissive temperature: transformants were only obtained i...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2011.02446.x
更新日期:2012-01-01 00:00:00
abstract::Sulfamate is an analogue of thiosulfate, and the sodium and potassium salts of sulfamic acid inhibited the chemolithoautotrophic growth on thiosulfate of Acidithiobacillus ferrooxidans and Halothiobacillus neapolitanus. The chemo-organotrophic growth of Paracoccus versutus on sucrose was similarly inhibited by sulfama...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2006.00437.x
更新日期:2006-11-01 00:00:00
abstract::In this report we propose a model of apical growth for streptomycetes. The apical tip is considered as a multilayered wall that expands by an inside-to-outside mechanism of growth. It is also assumed that each layer is made up of peptidoglycan blocks, each of them being the result of the biosynthetic activity of a wal...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1992.tb14063.x
更新日期:1992-12-15 00:00:00
abstract::The branched-chain amino acid permease Bap2p is a transport system for leucine, isoleucine, and valine in Saccharomyces cerevisiae, and its synthesis is regulated transcriptionally. However, the downregulation mechanisms of Bap2p have not been established. Here we demonstrate that the C-terminal region of Bap2p plays ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2001.tb09471.x
更新日期:2001-01-15 00:00:00
abstract::A Caulobacter crescentus rho::Tn5 mutant strain presenting a partially functional transcription termination factor Rho is highly sensitive to hydrogen peroxide in both exponential and stationary phases. The mutant was shown to be permanently under oxidative stress, based on fluorophore oxidation, and also to be sensit...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2009.01860.x
更新日期:2010-02-01 00:00:00
abstract::We have studied the single rRNA gene cluster from the Archaeon, Pyrococcus furiosus. This isolate grows optimally at 100 degrees C and is thus a hyperthermophile. In P. furiosus, transcription of 16S rRNA is subject to regulation over a 7.5-fold range in response to a 20-fold increase in growth rate. The single cluste...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1993.tb06379.x
更新日期:1993-08-01 00:00:00
abstract::Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2001.tb10809.x
更新日期:2001-08-21 00:00:00
abstract::Pseudomonas aeruginosa strains exhibiting a heterogeneous mode of growth against carbapenems have been described recently. This study investigated the underlying molecular mechanisms in four genetically unrelated P. aeruginosa clinical isolates that were previously characterized by population analyses as heterogeneous...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2007.00997.x
更新日期:2008-02-01 00:00:00
abstract::The ADP-ribosylating enzymes are encoded in many pathogenic bacteria in order to affect essential functions of the host. In this study, we show that Neisseria gonorrhoeae possess a locus that corresponds to the ADP-ribosyltransferase NarE, a previously characterized enzyme in N. meningitidis The 291 bp coding sequence...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnw181
更新日期:2016-09-01 00:00:00
abstract::Biogenic amines have undesirable physiological effects when absorbed at too high a concentration. Several kinds of food and beverages contain biogenic amines. Lactic acid bacteria can decarboxylate amino acids. Since winemaking involves the growth of lactic acid bacteria for malolactic fermentation, biogenic amines ma...
journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6968.2001.tb10643.x
更新日期:2001-05-15 00:00:00
abstract::To determine the existence of an acid stress response in Helicobacter pylori the global changes in the proteins synthesized by the bacterium when subjected to an acid stress were studied. H. pylori ATCC43504 previously adapted to pH 7 did not show an acid stress response as detected by the two-dimensional electrophore...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2002.tb11287.x
更新日期:2002-07-16 00:00:00
abstract::The sepsis caused by Vibrio vulnificus is characterized by an average incubation period of 26 h and a high mortality rate exceeding 50%. The fast growth and dissemination of V. vulnificus in vivo lead to poor clinical outcomes in patients. Therefore, elucidation of the proliferation mechanisms of this organism in vivo...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnw274
更新日期:2017-01-01 00:00:00
abstract::Helicobacter pylori genomes contain about 30 hop genes that encode outer membrane proteins. Helicobacter pylori hopQ alleles exhibit a high level of genetic diversity, and two families of hopQ alleles have been described. Type I hopQ alleles are found more commonly in cag-positive H. pylori strains from patients with ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2008.01368.x
更新日期:2008-12-01 00:00:00
abstract::Ovine footrot is a debilitating and highly infectious disease that is primarily caused by the Gram-negative, anaerobic bacterium Dichelobacter nodosus. The major antigens implicated in virulence are the type IV fimbriae and extracellular proteases. The fimbriae show sequence and structural similarity to other type IV ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6968.1996.tb08570.x
更新日期:1996-12-01 00:00:00
abstract::Methods for in vivo monitoring of redox changes in different cellular compartments have been developed in recent years, and are mostly based on redox-sensitive variants of the green fluorescent protein (GFP). However, due to the thermodynamic stability of the introduced reactive disulfide bond, these sensors are limit...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2010.01935.x
更新日期:2010-05-01 00:00:00
abstract::Occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in Japan and Korea. The tet(M) gene was detected in 34 Japanese and Korean isolates, which included Vibrio sp., Lactococcu...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/j.femsle.2004.06.026
更新日期:2004-08-01 00:00:00
abstract::The genus Phytomonas is responsible for many diseases in different crop plant species. The finding that chitin is an exposed cell surface polysaccharide in Phytomonas françai and the observation that chitinases can inhibit fungal growth raises expectations about the potential effect of plant chitinases on the P. franç...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/S0378-1097(03)00526-3
更新日期:2003-09-12 00:00:00
abstract::Legionella pneumophila, an intracellular parasite of macrophages and protozoa, requires iron for extra- and intracellular growth. In a new screen of a mutant library of L. pneumophila for strains defective for growth on agar media lacking supplemental iron, seven mutants were obtained. All of the mutants had a disrupt...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/j.femsle.2004.10.028
更新日期:2004-12-15 00:00:00
abstract::PhoP was previously shown to be important for Yersinia pestis survival in macrophage and under macrophage-induced stresses. In this work, a phoP disruptant of Y. pestis 201 was generated using the Red cloning procedure. The transcription profile of the wild-type Y. pestis was then compared with that of the phoP mutant...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/j.femsle.2005.06.053
更新日期:2005-09-01 00:00:00
abstract::Synthesis of nitrate reductase in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942 took place at a slow rate when the cells were incubated without a supply of inorganic carbon, but addition to these cells of CO(2)/bicarbonate or, in a Synechococcus strain transformed with a gene encoding a 2-oxoglutara...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/S0378-1097(03)00208-8
更新日期:2003-04-25 00:00:00
abstract::Two thermophilic bacteria, which are capable of growing on starch at 60-70 degrees C under anaerobic conditions, were isolated from a sugar refinery in Uelzen and from Solar lake in Israel. On the basis of their physiological characteristics they were identified as Clostridium thermohydrosulfuricum Uel 1 and C. thermo...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(90)90429-t
更新日期:1990-05-01 00:00:00
abstract::In order to obtain functional expression of PHA synthase gene phaC2 from Pseudomonas aeruginosa in Escherichia coli, the coding region of phaC2 was subcloned, including the ribosomal binding site, into pBluescript SK- collinear to the lac promoter. This plasmid pBHR71-C2 enabled functional expression of phaC2 in E. co...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1997.tb12767.x
更新日期:1997-12-01 00:00:00
abstract::Two bifunctional plasmid vectors pZG5 (7.45 kb) and pZG6 (6.95 kb), for gene transfer between Streptomyces spp. and Escherichia coli have been constructed by fusion of the multicopy broad-host-range Streptomyces plasmid pIJ350 with E. coli plasmids Bluescribe M13- (pZG5) or pUC18 (pZG6). Both plasmids possess several ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(91)90495-v
更新日期:1991-10-15 00:00:00
abstract::Phylogenetically diverse rhizobial strains endemic to Tunisia were isolated from symbiotic nodules of Lotus creticus, growing in different arid extremophile geographical regions of Tunisia, and speciated using multiloci-phylogenetic analysis as Neorhizobium huautlense (LCK33, LCK35, LCO42 and LCO49), Ensifer numidicus...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnaa084
更新日期:2020-06-01 00:00:00