Abstract:
:To determine the existence of an acid stress response in Helicobacter pylori the global changes in the proteins synthesized by the bacterium when subjected to an acid stress were studied. H. pylori ATCC43504 previously adapted to pH 7 did not show an acid stress response as detected by the two-dimensional electrophoretic pattern of 35S-labeled proteins when incubated at pH 3. This was probably due to the neutralization of the external medium by the action of urease. However, H. pylori DW504UreI-negative, a mutant strain unable to transport urea into the cell, showed a large number of proteins changed, as is typical in an acid stress response. Some of these proteins were identified by N-terminal sequencing.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Toledo H,Valenzuela M,Rivas A,Jerez CAdoi
10.1111/j.1574-6968.2002.tb11287.xkeywords:
subject
Has Abstractpub_date
2002-07-16 00:00:00pages
67-72issue
1eissn
0378-1097issn
1574-6968pii
S0378109702007899journal_volume
213pub_type
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