Abstract:
:Pseudomonas aeruginosa strains exhibiting a heterogeneous mode of growth against carbapenems have been described recently. This study investigated the underlying molecular mechanisms in four genetically unrelated P. aeruginosa clinical isolates that were previously characterized by population analyses as heterogeneously resistant against carbapenems. Mutant subpopulations of all four isolates had at least fourfold higher minimum inhibitory concentrations than those of native cells for imipenem and meropenem. The heterogeneous subpopulations, when compared with the respective native ones, had significantly increased transcription levels of the mexB and mexY genes (P<0.05), whereas transcription levels of the mexE gene remained unchanged. They also exhibited significantly decreased expression of the oprD gene (P<0.05) and decreased intensity of the protein band of the porin OprD. Upregulation of efflux systems, in part, and the decrease of OprD contribute to the heterogeneous growth against carbapenems in our P. aeruginosa clinical isolates.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Ikonomidis A,Tsakris A,Kantzanou M,Spanakis N,Maniatis AN,Pournaras Sdoi
10.1111/j.1574-6968.2007.00997.xsubject
Has Abstractpub_date
2008-02-01 00:00:00pages
36-9issue
1eissn
0378-1097issn
1574-6968pii
FML997journal_volume
279pub_type
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