Abstract:
:Twitching motility is a form of bacterial translocation over solid or semi-solid surfaces mediated by the extension, tethering, and subsequent retraction of type IV pili. These pili are also known to be involved in virulence, biofilm formation, formation of fruiting bodies, horizontal gene transfer, and protein secretion. We have characterized the presence of twitching motility on agar plates in Legionella pneumophila, the etiological agent of Legionnaires' disease. By examining twitching motility zones, we have demonstrated that twitching motility was dependent on agar thickness/concentration, the chemical composition of the media, the presence of charcoal and cysteine, proximity to other bacteria, and temperature. A knockout mutant of the pilus subunit, pilE, exhibited a total loss of twitching motility at 37 degrees C, but not at 27 degrees C, suggesting either the existence of a compensating pilus subunit or of another twitching motility system in this organism.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Coil DA,Anné Jdoi
10.1111/j.1574-6968.2009.01532.xsubject
Has Abstractpub_date
2009-04-01 00:00:00pages
271-7issue
2eissn
0378-1097issn
1574-6968pii
FML1532journal_volume
293pub_type
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journal_title:FEMS microbiology letters
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