Isozyme and morphological characteristics of the anaerobic fungus Piromyces mae isolated from the duodenum, rumen and faeces of sheep.


:Isolates of anaerobic fungi obtained from the rumen, duodenum and faeces of sheep were identified as Piromyces mae based on their morphological characteristics observed using light microscopy. There was no significant morphological variation among the isolates of P. mae from the rumen, duodenum and faeces. Isozymes of 12 isolates of P. mae (one each from the rumen, duodenum and faeces from 4 different sheep) were analysed by PAGE. A total of 12 isozymes were studied and 5 isozyme loci were successfully typed. They were malic enzyme, malate dehydrogenase, shikimate dehydrogenase, alpha-esterase and beta-esterase. All the isolates of P. mae regardless of whether they were from the rumen, duodenum or faeces or from different animals produced very similar isozyme banding patterns for each of the enzyme systems. The similar isozyme profiles of the isolates indicate that they are of the same species although they exist in different regions of the alimentary tract.


FEMS Microbiol Lett


Wong MV,Ho YW,Tan SG,Abdullah N,Jalaludin S




Has Abstract


1995-12-01 00:00:00














  • The IS1111 insertion sequence used for detection of Coxiella burnetii is widespread in Coxiella-like endosymbionts of ticks.

    abstract::Coxiella is a genus of obligate intracellular bacteria engaged in a variety of interactions with eukaryotes. The type species, Coxiella burnetii, infects several vertebrate species, including humans, and is the causative agent of Q fever. Multiple copies of a specific transposable element, the insertion sequence IS111...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Duron O

    更新日期:2015-09-01 00:00:00

  • Antigenicity of a viral peptide displayed on beta-galactosidase fusion proteins is influenced by the presence of the homologous partner protein.

    abstract::Several beta-galactosidase fusion proteins have been constructed containing the entire VP1 protein from foot-and-mouth disease virus (FMDV) [Corchero et al. (1996) J. Biotechnol. in press]. The antigenicity of the major immunodominant site A (13 amino acids in length) within the VP1 protein has been studied in competi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Corchero JL,Villaverde A

    更新日期:1996-11-15 00:00:00

  • Cellular transformation by human T-cell leukemia virus type I.

    abstract::Human T-cell leukemia virus type I (HTLV-I) is an oncogenic retrovirus that was isolated in 1980 from a patient with adult T-cell leukemia. From the numerous experiments using infected patient T-cells, transgenic mice and tissue culture transformation assays, the Tax protein has been determined to be the transforming ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Ressler S,Connor LM,Marriott SJ

    更新日期:1996-07-01 00:00:00

  • Concomitant expression of E. coli cytosine deaminase and uracil phosphoribosyltransferase improves the cytotoxicity of 5-fluorocytosine.

    abstract::The prodrug activation system formed by the E. coli codA gene encoding cytosine deaminase (CD) and 5-fluorocytosine (5-FC) developed for selective cancer chemotherapy suffers from a sensitivity limitation in many tumour cells. In an attempt to improve the CD/5-FC suicide association, we combined the E. coli upp gene e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tiraby M,Cazaux C,Baron M,Drocourt D,Reynes JP,Tiraby G

    更新日期:1998-10-01 00:00:00

  • Expression and purification of Clostridium perfringens beta-toxin glutathione S-transferase fusion protein.

    abstract::The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

    更新日期:1995-08-01 00:00:00

  • Sequence of the gene encoding type F neurotoxin of Clostridium botulinum.

    abstract::Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: East AK,Richardson PT,Allaway D,Collins MD,Roberts TA,Thompson DE

    更新日期:1992-09-15 00:00:00

  • Metabolic flux response to phosphoglucose isomerase knock-out in Escherichia coli and impact of overexpression of the soluble transhydrogenase UdhA.

    abstract::Blocking glycolytic breakdown of glucose by inactivation of phosphoglucose isomerase (Pgi) in Escherichia coli led to a greatly reduced maximum specific growth rate. Examination of the operational catabolic pathways and their flux ratios using [U-(13)C(6)]glucose-labeling experiments and metabolic flux ratio analysis ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Canonaco F,Hess TA,Heri S,Wang T,Szyperski T,Sauer U

    更新日期:2001-11-13 00:00:00

  • Isolation and characterization of Hena1 - a novel Erwinia amylovora bacteriophage.

    abstract::Fire blight, caused by plant pathogenic bacterium Erwinia amylovora, is one of the most important diseases of Rosaceae plants. Due to the lack of effective control measures, fire blight infections pose a recurrent threat on agricultural production worldwide. Recently, bacterial viruses, or bacteriophages, have been pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Besarab NV,Akhremchuk AE,Zlatohurska MA,Romaniuk LV,Valentovich LN,Tovkach FI,Lagonenko AL,Evtushenkov AN

    更新日期:2020-05-01 00:00:00

  • MJ0400 from Methanocaldococcus jannaschii exhibits fructose-1,6-bisphosphate aldolase activity.

    abstract::The central carbon metabolism is well investigated in bacteria, but this is not the case for archaea. MJ0400-His(6) from Methanocaldococcus jannaschii catalyzes the cleavage of fructose-1,6-bisphosphate (FBP) to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate with a V(max) of 33 mU mg(-1) and a K(m) of 430 m...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Samland AK,Wang M,Sprenger GA

    更新日期:2008-04-01 00:00:00

  • Characterization of human humoral responses to the major outer membrane protein and OMP2 of Chlamydophila pneumoniae.

    abstract::Chlamydophila pneumoniae infection is associated with a range of diseases including pneumonia, asthma and heart disease. Although an obligate intracellular pathogen, high levels of antigen-specific antibody are induced and serology is frequently used to diagnose these infections. Proteins targeted by the humoral respo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cunningham AF,Ward ME

    更新日期:2003-10-10 00:00:00

  • Gel electrophoretic analysis of penicillin-binding proteins of coagulase negative staphylococci.

    abstract::We analyzed gel electrophoretic banding patterns of penicillin-binding proteins (PBPs) of 16 type strains of coagulase-negative staphylococci (CNS). S. epidermidis, S. haemolyticus, S. saprophyticus, S. hominis, S. xylosus, S. simulans, S. warneri, S. capitis, S. saccharolyticus, S. auricularis, S. caseolyticus, S. ga...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kanda K,Hiramatsu K,Yokota T

    更新日期:1990-10-01 00:00:00

  • Biogenic amines in wines: role of lactic acid bacteria.

    abstract::Biogenic amines have undesirable physiological effects when absorbed at too high a concentration. Several kinds of food and beverages contain biogenic amines. Lactic acid bacteria can decarboxylate amino acids. Since winemaking involves the growth of lactic acid bacteria for malolactic fermentation, biogenic amines ma...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Lonvaud-Funel A

    更新日期:2001-05-15 00:00:00

  • Characterization of the gltF gene product of Escherichia coli.

    abstract::The glt operon of Escherichia coli comprises the structural genes for the glutamate synthase subunits (gltB and gltD) and gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between gltD and gltF contains a weak promoter and a translation initiation site for gltF. The GltF pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Grassl G,Bufe B,Müller B,Rösel M,Kleiner D

    更新日期:1999-10-01 00:00:00

  • Evidence that OmcB and OmpB of Geobacter sulfurreducens are outer membrane surface proteins.

    abstract::The c-type cytochrome (OmcB) and the multicopper protein (OmpB) required for Fe(III) oxide reduction by Geobacter sulfurreducens were predicted previously to be outer membrane proteins, but it is not clear whether they are positioned in a manner that permits the interaction with Fe(III). Treatment of whole cells with ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Qian X,Reguera G,Mester T,Lovley DR

    更新日期:2007-12-01 00:00:00

  • A peptidoglycan binding domain in the porin-associated protein (PAP) of Rhodospirillum rubrum FR1.

    abstract::The porin-associated protein of Rhodospirillum rubrum FR1 was found to contain a peptidoglycan binding motif. A partial fragment of 179 amino acids, obtained by cleavage of PAP with trypsin, Asp-N protease, and CNBr, was sequenced. Substantial sequence homology was found of the C-terminal part (residues 126-179) of po...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Neumann U,Schiltz E,Stahl B,Hillenkamp F,Weckesser J

    更新日期:1996-04-15 00:00:00

  • Separation of Pneumocystis carinii from the lung of the steroid-suppressed rat.

    abstract::Pneumocystis carinii pneumonia was induced in rats by chronic corticosteroid immunosuppression. The parasite was separated from virtually all contaminating host cells using the technique of unit gravity sedimentation. Cellular contamination was routinely below 0.02%. The same technique allowed partial separation of th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Taylor MB,Easmon CS

    更新日期:1990-06-15 00:00:00

  • Antifungal activity of a novel endochitinase gene (chit36) from Trichoderma harzianum Rifai TM.

    abstract::A novel 36-kDa endochitinase named chit36 has been isolated and characterized from Trichoderma harzianum Rifai TM. Partial amino acid sequences from the purified protein were used to clone the fungal cDNA, based on polymerase chain reaction with degenerate primers. The complete open reading frame encodes a 344-amino a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Viterbo A,Haran S,Friesem D,Ramot O,Chet I

    更新日期:2001-06-25 00:00:00

  • On surrogate methods for detecting lateral gene transfer.

    abstract::Surrogate methods for detecting lateral gene transfer are those that do not require inference of phylogenetic trees. Herein I apply four such methods to identify open reading frames (ORFs) in the genome of Escherichia coli K12 that may have arisen by lateral gene transfer. Only two of these methods detect the same ORF...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ragan MA

    更新日期:2001-07-24 00:00:00

  • Identification and characterization of a cross-reactive and a unique B-cell epitope on the hsp60 homologue from Neisseria gonorrhoeae.

    abstract::Two monoclonal antibodies (G6 and 7B), generated against a 63-kDa stress protein (GSP63) from Neisseria gonorrhoeae strain VP1, were used to investigate the antigenic heterogeneity of GSP63 among the Neisseriaceae and its antigenic relationship with the Hsp60 heat-shock protein family. Immunoblotting experiments demon...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pannekoek Y,Dankert J,van Putten JP

    更新日期:1992-11-15 00:00:00

  • Taxonomic status of putative Verticillum alboatrum isolates.

    abstract::Two fungal isolates, formerly classified as Verticillium alboatrum and proposed as forming the basis of a new sub-group ('Group 2') within the species, have been shown to be non-pathogenic to known hosts of V. alboatrum and, on the basis of molecular evidence, to be closely related to Verticillium psalliotae and Verti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Carder JH,Barbara DJ

    更新日期:1999-01-01 00:00:00

  • Quantification of class 1 integron abundance in natural environments using real-time quantitative PCR.

    abstract::Integrons are bacterial genetic elements capable of capturing and expressing potentially adaptive genetic material. Class 1 integrons constitute the most intensely studied group of these elements to date, mainly due to their well-established role in the acquisition and dissemination of antibiotic resistance genes in c...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Hardwick SA,Stokes HW,Findlay S,Taylor M,Gillings MR

    更新日期:2008-01-01 00:00:00

  • Production in vitro, on different solid culture media, of two distinct exopolysaccharides by a mucoid clinical strain of Burkholderia cepacia.

    abstract::The production of exopolysaccharides (EPSs) by a mucoid clinical isolate of Burkholderia cepacia involved in infections in cystic fibrosis patients, was studied. Depending on the growth conditions, this strain was able to produce two different EPS, namely PS-I and PS-II, either alone or together. PS-I is composed of e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cérantola S,Montrozier H

    更新日期:2001-08-07 00:00:00

  • Current status of defensins and their role in innate and adaptive immunity.

    abstract::Naturally occurring antimicrobial cationic polypeptides play a major role in innate and adaptive immunity. These polypeptides are found to be either linear and unstructured or structured through disulfide bonds. Among the structured antimicrobial polypeptides, defensins comprise a family of cysteine-rich cationic poly...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Raj PA,Dentino AR

    更新日期:2002-01-02 00:00:00

  • Existence and characterization of allelic variants of Sao, a newly identified surface protein from Streptococcus suis.

    abstract::Surface antigen one (Sao) is a newly identified protein from the major zoonotic pathogen, Streptococcus suis. In search of functional proteins related to the pathogenesis of Chinese S. suis 2 (SS2), unexpectedly, a variant of Sao protein was obtained. To test its prevalence in S. suis, PCR assay was adopted to address...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Feng Y,Zheng F,Pan X,Sun W,Wang C,Dong Y,Ju AP,Ge J,Liu D,Liu C,Yan J,Tang J,Gao GF

    更新日期:2007-10-01 00:00:00

  • Characterization of the membrane sensor PenJ for beta-lactam antibiotics from Bacillus licheniformis by amino acid substitution.

    abstract::The PenJ protein of the penicillinase gene (penP) expression system from Bacillus licheniformis is an antirepressor and membrane receptor for beta-lactam antibiotics. A putative beta-lactam antibiotic binding site including Ser402 and Lys405, which are homologous to the conserved sequence for the beta-lactam binding s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Takagi M,Ohta T,Johki S,Imanaka T

    更新日期:1993-06-01 00:00:00

  • Hydrogenase genes are uncommon and highly conserved in Rhizobium leguminosarum bv. viciae.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fernández D,Toffanin A,Palacios JM,Ruiz-Argüeso T,Imperial J

    更新日期:2005-12-01 00:00:00

  • Epitopes of serogroup B Neisseria meningitidis analysed in vitro and directly from cerebrospinal fluid.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wall RA,Davies HA,Borriello SP

    更新日期:1989-11-01 00:00:00

  • Laboratory-acquired lethal infections by potential bioweapons pathogens including Ebola in 2014.

    abstract::Researchers studying both cellular and viral disease agents in the laboratory have become infected since the early days of microbiology 150 years ago. However, in the early 21st century, new concerns about bioweapons being used to generate terror and also with a series of newly emerging or newly understood disease-cau...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Silver S

    更新日期:2015-01-01 00:00:00

  • Genotyping of clinical Serratia marcescens isolates: a comparison of PCR-based methods.

    abstract::The polymerase chain reaction (PCR)-based procedures of randomly amplified polymorphic DNA (RAPD) and repetitive element (RE)-based PCR were used to amplify total DNA prepared from each of 62 clinical Serratia marcescens isolates. Three different random primers, designated 1060, 1254 and 1283, were used individually i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Patton TG,Katz S,Sobieski RJ,Crupper SS

    更新日期:2001-01-01 00:00:00

  • Variability of Leishmania (Leishmania) infantum among stocks from immunocompromised, immunocompetent patients and dogs in Spain.

    abstract::Leishmania (Leishmania) infantum is the causative agent of both the cutaneous and visceral forms of leishmaniasis in southwest Europe; the dog is the main reservoir. In order to identify the L. (L.) infantum zymodemes present in Spain, a total number of 85 Leishmania stocks isolated from dogs (31), HIV-positive patien...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jiménez M,Ferrer-Dufol M,Cañavate C,Gutiérrez-Solar B,Molina R,Laguna F,López-Vélez R,Cercenado E,Daudén E,Blázquez J

    更新日期:1995-09-01 00:00:00