Abstract:
:Isolates of anaerobic fungi obtained from the rumen, duodenum and faeces of sheep were identified as Piromyces mae based on their morphological characteristics observed using light microscopy. There was no significant morphological variation among the isolates of P. mae from the rumen, duodenum and faeces. Isozymes of 12 isolates of P. mae (one each from the rumen, duodenum and faeces from 4 different sheep) were analysed by PAGE. A total of 12 isozymes were studied and 5 isozyme loci were successfully typed. They were malic enzyme, malate dehydrogenase, shikimate dehydrogenase, alpha-esterase and beta-esterase. All the isolates of P. mae regardless of whether they were from the rumen, duodenum or faeces or from different animals produced very similar isozyme banding patterns for each of the enzyme systems. The similar isozyme profiles of the isolates indicate that they are of the same species although they exist in different regions of the alimentary tract.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Wong MV,Ho YW,Tan SG,Abdullah N,Jalaludin Sdoi
10.1111/j.1574-6968.1995.tb07906.xsubject
Has Abstractpub_date
1995-12-01 00:00:00pages
9-14issue
1eissn
0378-1097issn
1574-6968pii
0378109795003515journal_volume
134pub_type
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