Isozyme and morphological characteristics of the anaerobic fungus Piromyces mae isolated from the duodenum, rumen and faeces of sheep.


:Isolates of anaerobic fungi obtained from the rumen, duodenum and faeces of sheep were identified as Piromyces mae based on their morphological characteristics observed using light microscopy. There was no significant morphological variation among the isolates of P. mae from the rumen, duodenum and faeces. Isozymes of 12 isolates of P. mae (one each from the rumen, duodenum and faeces from 4 different sheep) were analysed by PAGE. A total of 12 isozymes were studied and 5 isozyme loci were successfully typed. They were malic enzyme, malate dehydrogenase, shikimate dehydrogenase, alpha-esterase and beta-esterase. All the isolates of P. mae regardless of whether they were from the rumen, duodenum or faeces or from different animals produced very similar isozyme banding patterns for each of the enzyme systems. The similar isozyme profiles of the isolates indicate that they are of the same species although they exist in different regions of the alimentary tract.


FEMS Microbiol Lett


Wong MV,Ho YW,Tan SG,Abdullah N,Jalaludin S




Has Abstract


1995-12-01 00:00:00














  • Molecular host mimicry and manipulation in bacterial symbionts.

    abstract::It is common among intracellular bacterial pathogens to use eukaryotic-like proteins that mimic and manipulate host cellular processes to promote colonization and intracellular survival. Eukaryotic-like proteins are bacterial proteins with domains that are rare in bacteria, and known to function in the context of a eu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Frank AC

    更新日期:2019-02-01 00:00:00

  • Multiple restriction-modification systems are present in rumen treponemes.

    abstract::Type II restriction endonucleases were purified by heparin-sepharose followed by ion chromatography from Treponema strains. The results indicate that in addition to frequently cutting GATC-specific restriction enzymes, the tested strains also possess rarely cutting endonucleases. The purified restriction endonucleases...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Piknova M,Javorsky P,Pristas P

    更新日期:2005-10-01 00:00:00

  • High correlation between Chagas' disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area.

    abstract::The detection of Trypanosoma cruzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas' disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wincker P,Bosseno MF,Britto C,Yaksic N,Cardoso MA,Morel CM,Brenière SF

    更新日期:1994-12-15 00:00:00

  • Properties of the periplasmic nitrate reductases from Paracoccus pantotrophus and Escherichia coli after growth in tungsten-supplemented media.

    abstract::Paracoccus pantotrophus grown anaerobically under denitrifying conditions expressed similar levels of the periplasmic nitrate reductase (NAP) when cultured in molybdate- or tungstate-containing media. A native PAGE gel stained for nitrate reductase activity revealed that only NapA from molybdate-grown cells displayed ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gates AJ,Hughes RO,Sharp SR,Millington PD,Nilavongse A,Cole JA,Leach ER,Jepson B,Richardson DJ,Butler CS

    更新日期:2003-03-28 00:00:00

  • The Cyclic AMP-binding protein CbpB in Brucella melitensis and its role in cell envelope integrity, resistance to detergent and virulence.

    abstract::Brucella melitensis possesses an operon with two components: the response regulator OtpR and a putative cAMP-dependent protein kinase regulatory subunit encoded by the BMEI0067 gene. In the previous study, the function of OtpR has been studied, while little is known about the function of the BMEI0067 gene. Using a bio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Liu WJ,Dong H,Peng XW,Wu QM

    更新日期:2014-07-01 00:00:00

  • Nucleotide sequence of the nfaA gene encoding the antigen 8786 adhesive factor of enterotoxigenic Escherichia coli.

    abstract::The nucleotide sequence of a 714-bp DNA fragment containing the enterotoxic Escherichia coli (ETEC) adhesive factor 8786 structural gene, designated nfaA, revealed an open reading frame of 498 bp encoding a polypeptide of 166 amino acids. Primer-extension experiments showed that the nfaA gene is within a single transc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Aubel D,Darfeuille-Michaud A,Martin C,Joly B

    更新日期:1992-11-01 00:00:00

  • Serine racemase homologue of Saccharomyces cerevisiae has L-threo-3-hydroxyaspartate dehydratase activity.

    abstract::The NH(2)-terminal amino acid sequence of L-threo-3-hydroxyaspartate dehydratase from Pseudomonas sp. T62 showed significant similarity to that of the SRY1 gene product of Saccharomyces cerevisiae (serine racemase in yeast). SRY1 was cloned and expressed in Escherichia coli, and the gene product was purified and parti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wada M,Nakamori S,Takagi H

    更新日期:2003-08-29 00:00:00

  • Virulence regions and virulence factors of the ovine footrot pathogen, Dichelobacter nodosus.

    abstract::Ovine footrot is a debilitating and highly infectious disease that is primarily caused by the Gram-negative, anaerobic bacterium Dichelobacter nodosus. The major antigens implicated in virulence are the type IV fimbriae and extracellular proteases. The fimbriae show sequence and structural similarity to other type IV ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Billington SJ,Johnston JL,Rood JI

    更新日期:1996-12-01 00:00:00

  • Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.

    abstract::In Bacillus subtilis, the yoxA and dacC genes were proposed to form an operon. The yoxA gene was overexpressed in Escherichia coli and its product fused to a polyhistidine tag was purified. An aldose-1-epimerase or mutarotase activity was measured with the YoxA protein that we propose to rename as GalM by analogy with...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Duez C,Zervosen A,Teller N,Melkonian R,Banzubazé E,Bouillenne F,Luxen A,Frère JM

    更新日期:2009-11-01 00:00:00

  • Characterization of a Mycobacterium bovis BCG insertion sequence related to the IS21 family.

    abstract::The structure and distribution of a Mycobacterium bovis BCG insertion element of the IS21 family were investigated. Several IS21-like elements found in mycobacterial genomes were separated in four types, following their nucleic acid similarities. The M. bovis BCG IS21 element is highly similar to IS1533 (class I), 70%...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lefèvre P,Braibant M,Content J,Gilot P

    更新日期:1999-09-15 00:00:00

  • Extracellular proteolytic activation of bacteriolytic peptidoglycan hydrolases of Staphylococcus simulans biovar staphylolyticus.

    abstract::Staphylococcus simulans biovar staphylolyticus secreted two bacteriolytic peptidoglycan hydrolases as proproteins that were activated as they were processed by an extracellular sulphydryl protease. This processing resulted in the production of multiple molecular-mass forms of each enzyme. Cells from early exponential ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Neumann VC,Heath HE,LeBlanc PA,Sloan GL

    更新日期:1993-06-15 00:00:00

  • Production in vitro, on different solid culture media, of two distinct exopolysaccharides by a mucoid clinical strain of Burkholderia cepacia.

    abstract::The production of exopolysaccharides (EPSs) by a mucoid clinical isolate of Burkholderia cepacia involved in infections in cystic fibrosis patients, was studied. Depending on the growth conditions, this strain was able to produce two different EPS, namely PS-I and PS-II, either alone or together. PS-I is composed of e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cérantola S,Montrozier H

    更新日期:2001-08-07 00:00:00

  • Hydrogenase genes are uncommon and highly conserved in Rhizobium leguminosarum bv. viciae.

    abstract::A screening for hydrogen uptake (hup) genes in Rhizobium leguminosarum bv. viciae isolates from different locations within Spain identified no Hup+ strains, confirming the scarcity of the Hup trait in R. leguminosarum. However, five new Hup+ strains were isolated from Ni-rich soils from Italy and Germany. The hup gene...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fernández D,Toffanin A,Palacios JM,Ruiz-Argüeso T,Imperial J

    更新日期:2005-12-01 00:00:00

  • Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp.

    abstract::Transposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we showed the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jittawuttipoka T,Buranajitpakorn S,Fuangthong M,Schweizer HP,Vattanaviboon P,Mongkolsuk S

    更新日期:2009-09-01 00:00:00

  • The triazine hydrolase gene trzN from Nocardioides sp. strain C190: cloning and construction of gene-specific primers.

    abstract::Using oligonucleotides derived from the N-terminal sequence of a triazine hydrolase from Nocardioides sp. strain C190, two DNA fragments containing trzN were cloned into Escherichia coli and their nucleotide sequences were determined. The 456-amino acid polypeptide predicted from the 1356-bp trzN ORF displayed signifi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mulbry WW,Zhu H,Nour SM,Topp E

    更新日期:2002-01-02 00:00:00

  • DNA sequencing reveals bacterial communities in midgut and other parts of the larvae of Spodoptera exigua Hubner (Lepidoptera: Noctuidae).

    abstract::Gut microbiota has been suggested as an important component of host defense. However, microbiota in other parts of the body have rarely been characterized. In our present work, we give a detailed view of the body of Spodoptera exigua larvae, the midgut with intestinal contents (MG), midgut excluding intestinal content...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gao X,Li W,Luo J,Zhang L,Ji J,Zhu X,Wang L,Zhang K,Zhang S,Cui J

    更新日期:2020-02-01 00:00:00

  • Plasmid-mediated virulence genes in non-typhoid Salmonella serovars.

    abstract::Specific non-typhoid Salmonella serovars carry large virulence plasmids that promote sustained extra-intestinal infections. These plasmids all share a highly conserved 8-kb region containing the spv operon, consisting of the regulatory spvR locus and the four structural spvABCD genes. The SpvR protein belongs to the L...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Guiney DG,Fang FC,Krause M,Libby S

    更新日期:1994-11-15 00:00:00

  • Relationships between phosphatidylcholine content, chitin synthesis, growth, and morphology of Aspergillus nidulans choC.

    abstract::The phosphatidylcholine (PC) content of Aspergillus nidulans choC was varied by growing the auxotroph in medium containing various concentrations of choline chloride. Direct linear correlations were observed between PC content and in vivo chitin synthase activity, between in vivo chitin synthase activity and mean hyph...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Binks PR,Robson GD,Goosey MW,Trinci AP

    更新日期:1991-10-01 00:00:00

  • An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda Red.

    abstract::We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, lambda Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by lambda Red and large deletions of both 117- and 165-kbp regions could be gener...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fukiya S,Mizoguchi H,Mori H

    更新日期:2004-05-15 00:00:00

  • Intercellular communication by related bacterial protein toxins: colicins, contact-dependent inhibitors, and proteins exported by the type VI secretion system.

    abstract::Bacteria are in constant conflict with competing bacterial and eukaryotic cells. To cope with the various challenges, bacteria developed distinct strategies, such as toxins that inhibit the growth or kill rivals of the same ecological niche. In recent years, two toxin systems have been discovered - the type VI secreti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Braun V,Patzer SI

    更新日期:2013-08-01 00:00:00

  • Light inhibits rifampicin inactivation and reduces rifampicin resistance due to a cloned mycobacterial ADP-ribosylation gene.

    abstract::Rifampicin is a principal drug used to combat infections by mycobacteria and related organisms. Most strains of Mycobacterium are able to inactivate this antibiotic by ribosylation via an ADP-ribosylated intermediate. We found that this inactivation was inhibited by light at levels similar to those prevailing in labor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dabbs ER,Quan S

    更新日期:2000-01-01 00:00:00

  • Trichomonas vaginalis haemolysis: evidence of functional pores formation on red cell membranes.

    abstract::We have investigated the mechanisms used by Trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. Haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mM EGTA. Osmotic protection experiments using carbohydrates with different molecular diameters a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fiori PL,Rappelli P,Rocchigiani AM,Cappuccinelli P

    更新日期:1993-05-01 00:00:00

  • Characterization of dextran-producing Weissella strains isolated from sourdoughs and evidence of constitutive dextransucrase expression.

    abstract::The study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that Weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. In this study, several dextran-producing strains, Weissella cibari...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bounaix MS,Robert H,Gabriel V,Morel S,Remaud-Siméon M,Gabriel B,Fontagné-Faucher C

    更新日期:2010-10-01 00:00:00

  • Conserved and divergent functions of RcrRPQ in Streptococcus gordonii and S. mutans.

    abstract::In the dental caries pathogen Streptococcus mutans, an MarR-like transcriptional regulator (RcrR), two ABC efflux pumps (RcrPQ) and two effector peptides encoded in the rcrRPQ operon provide molecular connections between stress tolerance, (p)ppGpp metabolism and genetic competence. Here, we examined the role of RcrRPQ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shields RC,Burne RA

    更新日期:2015-08-01 00:00:00

  • Identification of a human cDNA clone that mediates adherence of pathogenic Neisseria to non-binding cells.

    abstract::Neisseria gonorrhoeae and Neisseria meningitidis are exclusively human pathogens. A crucial property of the pathogenicity of neisserial infection is the ability to adhere to human epithelial cells. Pili mediate adherence of these bacteria to target cells and thereby promote colonization and infection of mucosal surfac...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jonsson AB

    更新日期:1998-05-01 00:00:00

  • Role of PatS and cell type on the heterocyst spacing pattern in a filamentous branching cyanobacterium.

    abstract::Cell differentiation is one of the marks of multicellular organisms. Terminally specialised nitrogen-fixing cells, termed heterocysts, evolved in filamentous cyanobacteria more than 2 Gya. The development of their spacing pattern has been thoroughly investigated in model organisms such as Anabaena sp. PCC 7120. This p...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Antonaru LA,Nürnberg DJ

    更新日期:2017-08-15 00:00:00

  • Intracellular viability of toxigenic Corynebacterium diphtheriae strains in HEp-2 cells.

    abstract::Corynebacterium diphtheriae, generally considered an extracellular coloniser, was evaluated for its ability to enter and survive within HEp-2 monolayers by gentamicin protection assay. Intracellular viability of HC01 strain, isolated from endocarditis, was more expressive (2.59%) than observed in 241 (0.21%) and CDC-E...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hirata R,Napoleão F,Monteiro-Leal LH,Andrade AF,Nagao PE,Formiga LC,Fonseca LS,Mattos-Guaraldi AL

    更新日期:2002-09-24 00:00:00

  • Direct profiling of the yeast dynamics in wine fermentations.

    abstract::We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cocolin L,Bisson LF,Mills DA

    更新日期:2000-08-01 00:00:00

  • System-level modeling of acetone-butanol-ethanol fermentation.

    abstract::Acetone-butanol-ethanol (ABE) fermentation is a metabolic process of clostridia that produces bio-based solvents including butanol. It is enabled by an underlying metabolic reaction network and modulated by cellular gene regulation and environmental cues. Mathematical modeling has served as a valuable strategy to faci...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Liao C,Seo SO,Lu T

    更新日期:2016-05-01 00:00:00

  • Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects.

    abstract::A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain B...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Xue J,Liang G,Crickmore N,Li H,He K,Song F,Feng X,Huang D,Zhang J

    更新日期:2008-03-01 00:00:00